마이크로 공장을 위한 디지털 생산 시뮬레이션

박상호,정영상,송준엽,이승우,김동훈,이수훈,박종권 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

Existing manufacturing system has consumed too much energy, space and resource in micro parts manufacturing. To improve this, micro factory system is suggested. But it is difficult to get the high reliability in the assembly, production and inspection of the minute parts because the construction of the micro factory has been started just before. In this study, we will build the digital manufacturing simulation on the micro factory's process and verify the production and assembly process using this simulation.

培養鷄胚筋細胞分化에 미치는 細胞融合促進物質의 영향

朴映淳,朴鍾君,金賢玉,金鍾鎬,李東燁,金完柱 圓光大學校 1988 論文集 Vol.22 No.2

培養鷄胚筋細胸 分化에 미치는 促進物質의 영향을 알아보고자 2가양이온 (Ca2+, Mg2+) 胸胚抽出物 (EE), polyethylene glycol (PEG), 再生中인 도마뱀 (Lacertilia)꼬리 추출물 (BE)을 처리하여 細胸融合指數를 비교 조사하였다. 정상배양시의 細胸融合指數는 배양시작 70 시간에 최대에 달했으며 더이상 증가하지 않았고, Ca2+와 Mg2+ 처리할 경우에는 농도가 증가할수록 細胸融合指數는 저하되고, 근세포의 굵기도 정상세포보다 가늘고 핵이 모여있었다. Polyethylene Glycol (PEG)와 Blastemata Extract (BE) 처리군에서는 대조구와 큰 차이가 없는 것으로 나타났다. 鷄胚抽出物 (EE) 을 筋細胞分化에 필수적이나 과량으로 添加시에는 細胞融合은 억제되고 세포수는 증가되는것으로 나타났다. To study the effects of promoting factors on the differentiation of muscle cells, chick embryonic myoblasts were cultured with divalent cations (Ca2+, Mg2+) Chick embryo extract (EE), Polyethylene Glycol (PEG) and Blastema extract of Lacertilia (BE). Fusion index and Growth ratio were measured at various culture time. The fusion index of normal myoblast culture was reached maximum at 70 hours and the divalent cations (Ca2+, Mg2+) treated groups were decreased at higher concentration. The cellular formation of myoblasts treated with divalent cations was thiner and more aggregated between nuclei than that of normal myoblasts calture was. When polyethylene glycol (PEG) and Blastema extract of Lacertilia (BE) were treated to the myoblasts, tha fusion indices were similar to the control. Embryo Extract (EE) was seemed to be necessary for the fusion of chick embryonic myoblasts in culture, however, when excess of EE was added the fusion index was decreased and the number of cells was increased.

우리나라 유방아마에서 CYPIA1, EPHX 유전자 다형성의 역할

최지엽,유정희,이은옥,유근영,박수경,안세현,노동영,최국진,강대희 대한암예방학회 2002 Journal of cancer prevention Vol.7 No.3

시비방법별 벼 재배에 따른 전과정평가 방법을 적용한 환경영향 평가

신중두,임동규,김건엽,박문희,고문환,엄기철 한국환경농학회 2003 한국환경농학회지 Vol.22 No.1

Consoli가 설명한 것처럼 전과정평가의 개념 및 일반적인 정의에 따르면, 전과정평가는농업생산에 관련된 환경영향을 평가하는데 부합하는 방법이라고 생각되며, 특별히 전과정 평가방법인 Eco-indicator 95는 농업 체재에 대한 환경영향을 분석하는데 적절한 기법인 것으로 입정되었다. Eco-indicator 95 method를 이용하여 지구온난화 및 수계 부영양화와 관련된 시비체계에 따른 벼 재배에 대해 비교할만한 분석체계를 이루었다. 그렇지만 본 연구에서 전화정평가 항목에 기록된 모든 관련 정보가 환경 영향에 고려되지 않았기 때문에 Eco-indicator 95 method를 농업생산 체계에 적용할 때 몇 가지 난제에 부닥친다. Eco-indicator 95 method에 토양 및 자원의 이용과 같은 몇 몇 중요한 환경적인 문제가 포함되지 않았으며, 이 방법의 다른 문제는 환경 평가를 위하여 현장 정밀연구가 수행되지 않았다는 것이다. 그렇지만 본 연구에서 얻어진 생태지표 지수를 이용하여 벼 재배에 따른 시비방법 간의 차이점을 나타내기에 충분하다고 여겨지며, 가장 높은 생태지표 지수를 나타낸 화학비료를 시용한 구에서 가장 큰 환경영향이 관측되었고, 이러한 차이점은 주로 화학비료 투입에 의한 토양중의 높은 인산 함량의 축적 때문인 것으로 여겨진다. 분석한 시비체재는 특별히 지구온난화 보다는 수계의 부영양화의 환경적인 문제에 기여되는 것으로 나타났으므로 질소 시용비율 및 시용기술 이외에 액비 시용에 따른 양분 용탈이 수계 환경에 명확히 영향을 미치는 것으로 나타났다. The suitability of the Life Cycle Assessment (LCA) methodology to analyze'the environmental impact of rice cultivation with different fertilizing systems is investigated. The first part of an LCA is an inventory of parameters used and emissions released due to the system under investigation. In the following step, the Life Cycle Impact Assessment, the inventory data were analyzed and aggregated in order to finally get one index representing the total environmental burden. For the Life Cycle Impact Assessment (LCIA) the Eco-indicator 95 method has been chosen because this is well documented and regularly applied impact assessment method. The resulting index is called Ec -Indicator value. The higher the Eco-indicator value the stronger is the total environmental impact of an analyzed few system. The rice field experiment conducted in middle parts of korea was chosen as an example for the life cycle impact analysis. In this experiment the treatments were consisted of none fertilizer plot (NF), standard fertilizer plot (SF) applied chemical fertilizers based on soil chemical analysis before rice transplan ing, and efflux fertilized plot (EF) applied with pig wastes fermented as the same rates of SF plot as basis on total nitrogen content. The obtained Eco-indicator values were clearly different among the treatments in the rice trial. The total Eco-Indicator values for SF and EF have been observed 58 and 38% relative to the NF, respectively. For all the treatments the environmental effects of eutrophication contributed most to the total Eco-indicator value. The results appeared that the LCA methodology is basically suitable to assess the environmental impact associated with different fertilizer applications for rice cultivation. A comparative analysis of the fertilizing system's contribution to global warming and eutrophication is possible.

Contribution of Zinc-Dependent Delayed Calcium Influx via TRPC5 in Oxidative Neuronal Death and its Prevention by Novel TRPC Antagonist

Park, Sang Eun,Song, Ji Hoon,Hong, Chansik,Kim, Dong Eun,Sul, Jee-Won,Kim, Tae-Youn,Seo, Bo-Ra,So, Insuk,Kim, Sang-Yeob,Bae, Dong-Jun,Park, Mi-Ha,Lim, Hye Min,Baek, In-Jeoung,Riccio, Antonio,Lee, Joo- Springer US 2019 Molecular Neurobiology Vol.56 No.4

<P>Oxidative stress is a key mediator of neuronal death in acute brain injuries, such as epilepsy, trauma, and stroke. Although it is accompanied by diverse cellular changes, increases in levels of intracellular zinc ion (Zn<SUP>2+</SUP>) and calcium ion (Ca<SUP>2+</SUP>) may play a critical causative role in oxidative neuronal death. However, the mechanistic link between Zn<SUP>2+</SUP> and Ca<SUP>2+</SUP> dyshomeostasis in neurons during oxidative stress is not well-understood. Here, we show that the exposure of cortical neurons to H<SUB>2</SUB>O<SUB>2</SUB> led to a zinc-triggered calcium influx, which resulted in neuronal death. The cyclin-dependent kinase inhibitor, NU6027, inhibited H<SUB>2</SUB>O<SUB>2</SUB>-induced Ca<SUP>2+</SUP> increases and subsequent cell death in cortical neurons, without affecting the early increase in Zn<SUP>2+</SUP>. Therefore, we attempted to identify the zinc-regulated Ca<SUP>2+</SUP> pathway that was inhibited by NU6027. The expression profile in cortical neurons identified transient receptor potential cation channel 5 (TRPC5) as a candidate that is known to involve in the generation of epileptiform burst firing and epileptic neuronal death (Phelan KD et al. 2012a; Phelan KD et al. 2013b). NU6027 inhibited basal and zinc-augmented TRPC5 currents in TRPC5-overexpressing HEK293 cells. Consistently, cortical neurons from TRPC5 knockout mice were highly resistant to H<SUB>2</SUB>O<SUB>2</SUB>-induced death. Moreover, NU6027 is neuroprotective in kainate-treated epileptic rats. Our results demonstrate that TRPC5 is a novel therapeutic target against oxidative neuronal injury in prolonged seizures and that NU6027 is a potent inhibitor of TRPC5.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (10.1007/s12035-018-1258-7) contains supplementary material, which is available to authorized users.</P>

Attribution to Heterogeneous Risk Factors for Breast Cancer Subtypes Based on Hormone Receptor and Human Epidermal Growth Factor 2 Receptor Expression in Korea

Park, Boyoung,Choi, Ji-Yeob,Sung, Ho Kyung,Ahn, Choonghyun,Hwang, Yunji,Jang, Jieun,Lee, Juyeon,Kim, Heewon,Shin, Hai-Rim,Park, Sohee,Han, Wonshik,Noh, Dong-Young,Yoo, Keun-Young,Kang, Daehee,Park, Su Wolters Kluwer Health 2016 Medicine Vol.95 No.14

<▼1><P>Supplemental Digital Content is available in the text</P></▼1><▼2><P><B>Abstract</B></P><P>We conducted a heterogeneous risk assessment of breast cancer based on the hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) calculating the risks and population-based attributable fractions (PAFs) for modifiable and nonmodifiable factors.</P><P>Using matched case–control study design from the Seoul Breast Cancer Study and the national prevalence of exposure, the risks and PAFs for modifiable and nonmodifiable factors were estimated for total breast cancers and subtypes.</P><P>The attribution to modifiable factors was different for each subtype (luminal A, PAF = 61.4% [95% confidence interval, CI = 54.3%–69.8%]; luminal B, 21.4% [95% CI = 18.6–24.9%]; HER2-overexpression, 59.4% [95% CI = 47.8%–74.3%], and triple negative tumors [TNs], 27.1% [95% CI = 22.9%–32.4%)], and the attribution to the modifiable factors for the luminal A and HER2-overexpression subtypes was higher than that of the luminal B and TN subtypes (<I>P</I> heterogeneity ≤ 0.001). The contribution of modifiable reproductive factors to luminal A type in premenopausal women was higher than that of the other subtypes (18.2% for luminal A; 3.1%, 8.1%, and −3.1% for luminal B, HER2-overexpression, and TN subtypes, respectively; <I>P</I> heterogeneity ≤ 0.001). Physical activity had the highest impact preventing 32.6% of luminal A, 14.5% of luminal B, 38.0% of HER2-overexpression, and 26.9% of TN subtypes (<I>P</I> heterogeneity = 0.014). Total reproductive factors were also heterogeneously attributed to each breast cancer subtype (luminal A, 65.4%; luminal B, 24.1%; HER2-overexpression, 57.9%, and TN subtypes, −3.1%; <I>P</I> heterogeneity ≤ 0.001).</P><P>Each pathological subtype of breast cancer by HRs and HER2 status may be associated with heterogeneous risk factors and their attributable risk, suggesting a different etiology. The luminal B and TN subtypes seemed to be less preventable despite intervention for alleged risk factors, even though physical activity had a high preventable potential against breast cancer.</P></▼2>

Progressive altered expression of activator protein-1(AP-1) transcription factor proteins, Fra-2 and JunB, in hamster cholangiocarcinogenesis

Dong Yeob Park,Byung Il Yoon 대한인수공통전염병학회 2008 창립총회 및 학술대회 초록집 Vol.2008 No.1

Development of Fatigue Cracking Prediction Model for Flexible Pavements

Dong-Yeob Park,Neeraj Buch,Young-Chan Suh 대한토목학회 2001 KSCE journal of civil engineering Vol.5 No.4

Fra-2 and JunB are overexpressed in the dysplastic bile ducts and cholangiocarcinomas with a high correlation in their expression

Dong-Yeob Park,Jung-yeon Yi,Byung-IL Yoon 한국실험동물학회 2008 한국실험동물학회 학술발표대회 논문집 Vol.2008 No.-

경남지역에서 도축우 및 돼지의 근육내 잔류항균물질 검색

박동엽 ( Dong Yeob Park ),양평섭 ( Pyeong Seob Yang ),남창우 ( Chang Woo Nam ),황보원 ( Bo Won Hwang ),김원규 ( Won Gyu Kim ),조상래 ( Sang Rae Jo ),김도경 ( Toh Kyung Kim ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.3

The present studies were carried out to determine antibiotics residues in pork and beef muscles by EEC-4-plate and HPLC. A total of 2,534 samples of pork muscles and 1,070 samples of beef muscles from slaughter houses were collected in Gyeongnam area from January to December, 2001. The results were summarized as follows; 1. Recovery rates of TCs, Sulfa drug, Penicillin G from fortified pork and beef muscles ranged as 68.79~98.24%, 78.21~94.58% and penicillin G 63.35~67.24% respectively, by HPLC. 2. Antibiotics residues were detected in 36 sample(1.42%) of pork muscles, 29 sample (2.71%) of beef muscles by EEC-4-plate. 3. Detection rate of antibiotic residues 14 samples(0.55%) and 26 samples(2.43%), in pork and beef muscles, respectively by HPLC. Concentration of residues in 22 sample(2.06%) of beef muscle were higher than tolerance level in korea. 4. Antibiotics detected were sulfamethazine(47.37%), tetracycline(15.79%), oxytetracycline(15.79%), penicillin G(15.79%), sulfamerazine(5.26%) in pork muscle samples and oxyteracycline(37.21%), penicillin G(30.23%), sulfamethazine(20.93%), tetracycline(4.65%), sulfamerazine(2.33%), sulfadimethoxine(2.33%), sulfaquinoxine(2.33%) in beef muscle samples.