High-performance Liquid Chromatographic Analysis for Quantitation of Marker Compounds of Artemisia capillaris Thunb

( Kyung Min Park ),( Ying Li ),( Bora Kim ),( Haiyan Zhang ),( Kyong Hwangbo ),( Dong Gen Piao ),( Mei Juan Chi ),( Mi Hee Woo ),( Jae Sue Choi ),( Je Hyun Lee ),( Dong Cheul Moon ),( Hyeun Wook Chang 영남대학교 약품개발연구소 2013 영남대학교 약품개발연구소 연구업적집 Vol.23 No.0

Two stable high-performance liquid chromatography (HPLC) methods were developed that could quantitatively analyze 10 major marker compounds of Artemisia capillaris Thunb and could also distinguish among `Injinho` and `Myeon-injin` and `Haninjin`--A. capillaris collected in autumn, A. capillaris collected in spring and A. iwayomogi, which can be misused as `Injinho` in Korean herbal drug markets. The first HPLC method was a reversed-phase chromatography using a C18 column with an isocratic solvent system of phosphoric acid (0.05%) and acetonitrile at the flow rate of 1.0 mL/min, ultraviolet (UV) detection wavelength at 254 nm and column temperature at 40°C. Calibration andquantitation were made by using acetaminophen as an internal standard (I.S-A) and chlorogenic acid (1) was determined within 20 min. The second HPLC method was a reversed-phase chromatography using a C18 column with a gradient solvent system of phosphate buffer (0.015 M, pH 6) and acetonitrile at the flow rate of 1.0 mL/min, UV detection wavelength at 254 nm and column temperature at 40°C. Calibration and quantitation were made by using ethylparaben as an internal standard (I.S-B) and 3,5-di-O-caffeoylquinic acid (2), 3,4-di-O-caffeoylquinic acid (3), 4,5-di-O-caffeoylquinic acid (4), hyperoside (5), isoquercitrin (6), isorhamnetin 3-O-robinobioside (7), isorhamnetin-3-O-galactoside (8), isorhamnetin-3-O-glucoside (9) and scoparone (10) were determined within 60 min. Pattern recognitionanalysis of data from the 60 samples classified them clearly into three groups. These assay methods could be applied for QA/QC of A. capillaris and Artemisia iwayomogi.

경북 천생산, 유학산, 숲데미산지역의 현존식생 및 식물군락

김종현,박강민,강치욱,이종운 嶺南大學校基礎科學硏究所 2001 基礎科學硏究 Vol.21 No.-

From April to October 2001, we studied on vegetation of 60 plots through the methods of Braun-Blanquet at Cheonsaengsan, Yuhaksan and Supdemisan in Gyeongbuk. In whole area of Cheonsaengsan, Yuhaksan, Supdemisan we gave the grades based on the criteria of ministry of environment, and described an actual vegetation map based on physiognomy. Through the methods of Braun-Blanquet it showed 6 communities at Cheonsaengsan, 5 communities at Yuhaksan, 6 communities at Supdemisan and total appearance of 128 species and covered with 50∼95% of tree layer, 20∼90% of shrub layer, 20∼90% of herb layer. The vegetation in actual vegetation map divided by physiognomy were 8 communities at Cheonsaengsan, 8 communities at Yuhaksan, 10 communities at Supdemisan. The vegetation with more than vegetation grade Ⅲ was not appeared and it means that this area needs continuous conservation plan.

고강도 콘크리트 디스크를 이용한 혼합모드 파괴에 관한 연구

진치섭,김희성,박현재,김민철 부산대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.59 No.-

RILEM 위원회가 제시한 3점 휨 시험은 하중-변위곡선 형상이 불규칙하고 안정된 균열 발생 후 최종균열이 발생하기 때문에 정확한 파괴인성을 구하는 것은 어렵다. 그러나, 디스크 시험은 균열 개시 하중만 알면 쉽게 파괴인성을 구할 수 있다. 중앙에 노치가 있는 고강도 콘크리트 디스크를 실험하여 실험결과와 유한요소에 의한 결과를 비교하였다. 또한, 실험에 의한 파괴 포락선과 이론에 의한 파괴 포락선도 비교하였다. It is difficult to obtain accurate fracture toughness values by RILEM committees proposed three point bend test(TPB) because the shape of load-deflection curve is irregular and final crack propagation of occurs after some slow stable cracking. However, for disk test, fracture toughness is easily obtained from crack initiation load. We examined the center notched high strength concrete disk and the experimental results were compared with the results by finite element analysis(FEA). Also, we compared experimental

다형성교아세포종을 동반한 Von Recklinghausen氏 병 1예

이현개,이종수,백민우,이승재,박효일,지제근 인제대학교 1981 仁濟醫學 Vol.2 No.4

가족성으로 신경섬유종증에 동반되는 중추신경계의 종양으로 수막종, 신경교종, 신경초종 등이 있으며 이들은 드물지 않게 보고되었다. 그러나 신경교세포에 생기는 종양 중 신경섬유종증에 동반되어 대뇌에 생긴 다형성교아세포종은 매우 드물다. 본 교실에서 가족성으로 발생한 신경섬유종증의 환자에 동반된 다형성교아세포종을 경험하였으며 가족중 부친은 von Recklinghausen씨 병을, 또 두 자녀는 다발성 cafe´ au lait spots를 가지고 있었다. The tumors of the central nervous system associated with familial neurofibromatosis are meningioma, glioma, and neurilemmoma, which are not uncommon. Of the glial tumors of the central nervous system, the association of cerebral glioblastoma multiforme with neurofibro matosis is very rare. We have experienced the unusual coincidence of neurofibromatosis and glioblastoma multiforme in a 33 year old man, whose father was afflicted with von Recklinghausen's disease and whose son and daughter had also multiple cafe´ au lait spots on their bodies.

경북 유학산, 천생산과 성암산 일대의 식물군락유형 분석

박강민,김종현,정현아,강치욱,이종운 嶺南大學校基礎科學硏究所 2002 基礎科學硏究 Vol.22 No.-

At the area of Yuhak, Cheonsaeng and Seongam mountains in Kyeongbuk, we studied the vegetation patterns with 75 plots on the base of Braun-Blanquet methods from March to October 2002. For the analysis of community patterns with 75 plots we used interspecies Affinity analysis method, DECORANA and TWINSPAN. The investigated area was divided into 17 communities with 177 species, and 20∼100% of treelayer, 10∼95% of shrublayer and 20∼100% of herblayer. By toe method of affinity analysis, it showed a big community with 86 species, but divided into 6 small groups by ordination and classification methods. Although it appeared as one group by the affinity test, the area divided into two groups by classification method at second level with eigenvalue of 0.320 and 0.430. It shows that the area of Yuhak, Cheonsaeng and Seongam mountains have own properties and in the process of succession.

경북 천생산, 유학산, 숲데미산지역의 식물군락유형

박강민,김종현,강치욱,이종운 嶺南大學校基礎科學硏究所 2001 基礎科學硏究 Vol.21 No.-

From April to October 2001, we studied on vegetation of 60 plots through the methods of Braun-Blanquet at Cheonsaengsan, Yuhaksan and Supdemisan in Gyeongbuk. Through the methods of interspecific affinity analysis, DECORANA and TWINSPAN the patterns of plant communities of 60 were analysed. It showed 6 communities at Cheonsaengsan, 5 communities at Yuhaksan, 6 communities at Supdemisan and total appearance of 128 species and covered with 50∼95% of tree layer, 20∼90% of shrub layer, 20∼90% of herb layer. As the result of interspecific affinity analysis it showed one big specific complex composed with 52 species and the 60 plots were divided into 4 groups by classification and ordination. One big species complex as a result of affinity analysis and eigenvalue of 0.303 at first step by classification indicate that Cheonsaengsan, Yuhaksan and Supdemisan are in developing stage of succession.

쇄석 골재의 광물학적 및 화학적 특성과 알칼리-실리카 반응성

전쌍순,박현재,이효민,진치섭 釜山大學校生産技術硏究所 2002 生産技術硏究所論文集 Vol.61 No.-

본 연구에서는 쇄석 골재의 광물학적 및 화학적 특징과 알칼리-실리카 반응에 대한 골재의 유해 가능성을 연구하였다. 이를 위하여 알칼리-실리카 반응을 유발하는 광물들의 포함여부 및 이에 따라 잠재적으로 조기 열화현상을 유발할 수 있는 암석을 유형별(화성암, 변성암, 퇴적암 및 탄산염암)로 골재를 수집하였다. 암종 별로 선별하여 수집한 골재 내의 알칼리 반응성 유해 물질 존재 여부 확인 X선 회절분석(XRD)과 편광현미경에 의한 광물학적 방법을 사용하였다. 알칼리-실리카 반응성 유무해 판단을 ASTM C 227의 Mortar-Bar 시험방법에 의해 평가 분석하였고, 주 화학성분은 X선 형광분석기(XRF)로 실시하였다. In Korea, due to the shortcomings of natural aggregates and increasing needs of crushed stones, it is necessary to examine the alkali-silica reaction of the crushed stones. The purpose of this study is to analyze petrographic, chemical properties and alkali-silica reaction of crushed stones. This study was performed to investigate the alkali-silica reaction of crushed stones using chemical analysis, physical properties, XRD, XRF and mortar-bar method. As a result, most of test aggregates didn't show any reaction, but many test aggregates have common deleterious mineral. Therefore, there exists the possibility of chemical reaction in petrographic point of view.

관상동맥내 스텐트 삽입후 발생한 관상동맥류 2례

제인수,허민영,문치숙,이재호,정수룡,조길현,이영민,김대경,김두일,김동수 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.3

Coronary artery aneurysms are detected with increasing frequency because of the routine use of coronary angiography in the diagnosis and management of coronary artery disease. Although some coronary artery aneurysms are congenital or inflammatory, most are atherosclerotic. The prognosis of atherosclerotic coronary artery aneurysm may depend on the morphology of the aneurysm or the presence of coexisting obstructive disease. Coronary artery aneurysms have been also reported to occur after ballon angioplasty, directional athrectomy, laser angioplasty, and stent implantation with a frequency of 2% to 4%. The long-term outcome of post-intervention coronary artery aneurysm is unknown. We report two cases of the development of a coronary aneurysm 6 months after stent implantation as a treatment of severe obsructive coronary artery disease.

In situ 고상 에피택시 방법에 의한 CoSi₂/Si(111)구조의 형성

이정주,강민성,김현수,최치규,이운환,이종덕,이영백,김건호,이정용 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

초고진공에서 공상에피택시(solid-phase epiyaxy, SPE) 방법으로 CoSi₂를 Si(111)기판 위에 에피택시 성장시켰다. 2MeV⁴He++ ion후방산란 분광기와 사중결정 x-선 회절기 및 고분해 투과전자 현미경을 이용하여 성장된 CoSi₂의 상과 조성, 결정성, 그리고 계면의 미시구조를 조사하였다. 초고진공상태의 실온에서 Si(111)-7X7기판에 Co를 약 20 ~50 ?? 중착한 후 SPE에 의하여 실리사이드를 형성시키는 경우 600℃에서는 B-형의 CoSi₂가, 그리고 700℃에서는 A형의 CoSi₂가 선택적으로 에피택시 성장하였으며, 정합성은 B-CoSi₂[110]//Si[110] and CoSi₂(111)//Si(111)이, 그리고 700℃에서는 A-CoSi₂[110]//Si[110], CoSi₂(002)//Si(002)을 나타내었다. Epitaxial films of CoSi₂were grown on Si(111) substrates by in situ solid-phase epitaxy in a ultrahigh vacuum. The phase, the chemical composition, the crystallinity, and the microstructure of the Silicide/Si interface were investigated by 2-MeV⁴He++ ion-backscattering spectrometry, quadruple crystal X-ray diffractometry, and high-resolution transmission eletron micorscopy. High-quality films of either B-type or A-type CoSi₂ could be grown selectively on Si(111) substrates by depositing ~ 20 ~ 50 ?? of Co on a Si(111)-7X7 substrate followed by in situ annealing at 600℃ or 700℃ for 10 min. The matching face relationships are CoSi₂[110]//Si[110] and CoSi₂(111)//Si(111) by B-type CoSi₂ and CoSi₂[110]//Si[110]//Si[110] and CoSi₂(002)//Si(002) for A-type CoSi₂.

비장경색이 동반된 쯔쯔가무시병 1예

오종찬,이범주,장재현,원경준,박경희,김동민,박치영,정춘해 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.1

Tsutsugamushi disease is an acute febrile illness caused by Orientie. tsutsugsmushi. It is characterized by fever, myalgia, lymphadenopathy, and rash. And it can be easily diagnosed by characteristic eschar and serologic testing. Nearly all of the patients with tsutsugamushi disease improve with antibiotics such as doxycycline. However, the fatality rate of untreated cases is seven to ten percent. Splenic infarction is not common disease which is caused by occlusion of the main splenic artery or any of its branches. It is mainly caused by emboli that arise from cardiovascular disease, but sometimes by regional thrombosis associated with leukemia, myeloproliferative disorder, sickle cell anemia, pancreatitis, splenic torsion and septicemia. We experienced one case of tsutsugamushi disease with splenic infarction, which was improved after doxycycline treatment finished.