Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

백반증의 PUVA치료반응에 관한 연구(제1보)

박장규,성열훈,성범진,김영호,이증훈 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.2

The authors evaluated response to PUVA therapy in 44 vitiligo petients who did'nt show the spontaneous re-pigmentation. In the responded group to PUVA therapy after systemic administration of 8-methoxy psoralen, average MPD of female patients was 3.4 times higher than males, interestingly. Number of irradiation of UVA was similar between male and female, but total doses of female patients group were about 2 times than males till the first re-pigmented spots were noted. The response to PUVA therapy was quite different according to duration of disease and site of lesion. Therefore, we suppose that it is necessary to consider sex of patient, duration of disease, location of vitiligo before making a plan of PUVA therapy.

Effect of two different calcium hydroxide paste removal techniques on apical leakage : an electrochemical study

Park, Chan-Je,Jeon, Kyung-A,Kwon, Ho-Beom 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.3

본 연구의 목적은 근관내 약제로 사용되는 수산화칼슘의 제거방법이 근관충전 후 치근단 미세누출에 미치는 영향을 알아보기 위함이다. 70개의 발거된 단근치를 엔진구동형 ProFile로 근관형성한 후 수용성 수산화칼슘 제제를 1주일간 적용하고 두 군으로 나누어 각기 다른 방법으로 제거하였다. A군은 NaOCI로 근관세정만을 시행하였으며, B군은 근관형성 시 사용된 file보다 한 단계 큰 크기의 ProFile을 사용하여 근관을 재형성한 후 NaOCI로 세척하였다. 근관충전 후 전기화학적 방법으로 24일 동안 치근단 누출을 측정, 비교하였다. 본 연구의 결과 수산화 칼슘 제거를 위한 두가지 방법은 근관충전후 치근단 미세누출에 있어서 차이가 없음을 알 수 있었다. This study evaluated the effect of two different calcium hydroxide (Ca(OH)_(2)) paste removal techniques on the apical leakage of canals obturated with gutta percha cones and sealer after removing a Ca(OH)_(2) dressing using an electrochemical method. Seventy extracted single-rooted teeth were instrumented on with Profile rotary files under NaOCI irrigation. Fifty-eight canals were filled with calcium hydroxide paste, which was then removed using one of the following two techniques. In group A, calcium hydroxide was removed using only NaOCI irrigation, and in group B, the canals were re-prepared with a Profile rotary files-one size larger than the previous instrument and were irrigated with NaOCI. In both groups, the root surfaces were coated twice with nail varnish from CEJ to an area 4㎜ away from the apex after canal obturation. Apical leakage was measured using an electrochemical method for 24 days. All the specimens showed leakddffqage that increased markedly in the first three days. There was no significant difference between the two groups (p > 0.05). The effect of two calcium hydroxide paste removal techniques on the apical leakage was not different during a short period.

불량행위 청소년의 가족 폭행에 관한 사회 정신의학적 고찰(Ⅳ)

박범룡,류영호,조동환,김정기 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.3

연구목적: 이 연구는 청소년의 가족 폭행에 대하여 조사하고 가족폭행의 관점에서 불량행위 청소년의 특성을 규명하고자 하였다. 방 법: 저자들은 불량행위 청소년 238명, 범죄 청소년 209명, 그리고 부산시대 고등학교 학생 230명을 대상으로 설문지, 다면적 인성 검사(Minnesota Multiphasic Personality Inventory), 그리고 한국판 부모-자녀 결합도 검사(Parental Bonding Instrument0 등의 도구를 사용하여 조사하였고, 그것으로 세집단을 Chi-square test, T-test, 그리고 ANOVA를 사용하여 비교 분석하였다. 결 과: 대개의 경우 불량행위 청소년의 사회경제적인 상태가 학생군보다 더 불량하였고, 범죄군보다는 더 양호하였다.(p<.05) 일반적으로 폭행가해자들은 손위형제였고, 신체적으로도 피해자보다 더 강했고, 형제간의 우애는 좋지 않았다. 학생군과 비교할 때 존속에 대한 폭행을 포함한 폭행의 심각성은 불량행위 청소년과 범죄 청소년에서 존재하고 있었다. 다면적 인성검사와 부모-자녀 결합도 검사의 결과, 가족폭행군과 비폭행군을 비교할 때, 불량행위 청소년군의 경우에는 Sc척도와 Passive aggresive index에서 학생군의 경우에는 아버지 과보호에서 의미있는 차이를 보였지만 그외의 척도에서는 의미있는 차이를 찾을 수가 없었다. 결 론: 일반적으로 가족폭행의 특성으로도 기존의 사회 정신의학적 고찰의 결과와 같이 불량행위 청소년군이 학생군과 범죄청소년군의 중간특성을 가지고 있는 것으로 나타났으며 가족 폭행 문제의 심화를 막기 위하여 이 집단에 대한 적절한 개입이 필요할 것으로 생각되어진다. Objectives : This research was performed to identify family violence by male adolescents, and to examine the characteristics of male adolescent delinquents in terms of domestic violence. Methods : We surveyed 677 male adolescents including 238 delinquents from BBS(Big brothers and sisters) in Pusan, 209 criminal adolescents from adolescent correctional institution in Pusan, 230 high school students through questionnaire, MMPI(Minnesota Multiphasic Personality Inventory), and PBI(Parental Bonding Instrument), and then compared these three groups by Chi-square test, T-test, and Anova. Results : In most cases, male delinquent adolescents were placed in poorer socio-economic conditions than those of students, but in better socio-economic conditions than those of criminal adolescents. In general, the assaulters were senior family members, physically stronger than sufferers, and have bad brotherliness. Compared with normal students, the serious problem of violence including violence to the ancestors in delinquent and criminal adolescents. On results of MMPI and PBI test, there were significant difference between violent group and non-violent group on the Sc score and Passive aggressive index in adolescent delinquents and parental overprotection in normal students. Conclusion : Generally the characteristics of family violence by male delinquent adolescents were intermediate between those of criminal adolescent and students, these results were same as those of previous socio-psychiatric study, and so the intervention in criminal adolescents is needed for the prevention of family violence.

암면재배 수박의 품종별 당함량 비교

박순기,이범선,최진호,김월수,정순주 전남대학교 농업과학기술연구소 2000 農業科學技術硏究 Vol.35 No.-

The fruit qualities and sugar contents of hydroponically grown watermelon cultivars were investigated in order to obtain informations for nutriculture of watermelon in glass house. The fruit circumference of Mudeungsan watermelon was increased from 30 to 38 days after pollination, and periods of fruit development was longer than that of other cultivars. Total soluble solid content in fruit flesh of watermelon was high in Samboggool, Gwigongja, Dalgona, Gamro, Mudeungsan, Olimpia, Hwangto and Geumro in order. Maltose content was higher in Gwigongja and Mudeungsan watermelon cultivar Sucrose content was higher in Samboggool, Gwigongja and Dalgona watermelon cultivar than that of other watermelon cultivars. Glucose content was much higher in Mudeungsan watermelon cultivar while very lower in Samboggool, Gwigongja and Dalgona watermelon cultivar than that of other watermelon cultivars. Fructose content was higher in Hwangto, Gamro, and Mudeungsan watermelon cultivar while was lower in Gwigongja watermelon cultivar than that of other watermelon cultivars. Maltose, glucose, and fructose content in fruit fresh of watermelon was higher in Mudeungsan watermelon than that of other watermelon cultivars. Contents of maltose, fructose and glucose in fruit flesh of watermelon were higher in Mudeungsan watermelon than those of other cultivars.

접착제 미 사용시 치면열구전색제로서의 유동성 복합레진 평가

권호범,김명진,신철환,김지연,박기태 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.2

본 연구의 목적은 유동성 복합레진을 치면열구전색제로 사용할 수 있는지 여부를 평가하는 것이다. 세 종류의 유동성 복합레진(Filtek Flow, Tetric Flow, Charmfil Flow)과 한 종류의 filled sealant(Ultraseal XT plus)가 사용되었다. 주사전자 현미경을 이용하여 세 종류의 유동성 복합레진 및 filled sealant의 resin tag 형성 양상을 비교하였다. 미세누출 평가를 위해 발거된 사람의 소구치 54개를 각각 18개씩 무작위로 세 군으로 배분하였다. 각 군마다 통상의 filled sealant 와 한 가지씩의 유동성 복합레진을 교합면 열구에 도포하였다. 치아들은 thermocycling(5˚ ± 2℃와 55˚ ± 2℃ 사이에서 30초씩 1200회 시행) 후 48시간 동안 1% methylene blue 용액에 보관하였다. 각각의 치아를 절단하여 미세누출 정도를 관찰하였다. 세 종류의 유동성 복합레진 및 filled sealant는 유사한 resin tag 형성양상을 보였다. 세 종류의 유동성 복합레진은 각각 filled sealant보다 현저히 더 많은 미세누출을 보였다. 세 종류의 유동성 복합레진 간의 미세누출 정도는 유사하였다. 유동성 복합레진은 통상의 filled sealant와 비교해볼 때 보다 많은 미세누출을 야기할 수 있으므로 치면열구전색제로 적절하지 않다. The aims of this study were to determine if flowable composites can be used as pit and fissure sealants without bonding agents. Three flowable composites(Filtek Flow, Tetric Flow, Charmfil Flow) and a filled sealant (Ultraseal XT plus) were used. The pattern of resin tag formation in the four sealant meterials were compared using SEM. For the microleakage assessment, 54 extracted human premolar teeth were randomly divided into 3 groups containing 18 premolars each. In each group, a conventional filled sealant and one of the three flowable composites were applied to occlusal fissures. The teeth were thermocycled(1200 cycles between 5˚± 2℃ and 55˚± 2℃ with a dwell time of 30 seconds) and immersed in a 1% methylene blue solution for 48 hours. Each tooth was sectioned and examined to determine the extent of dye penetration. Three flowable composites and a filled sealant showed a similar resin tag formation pattern. The three flowable composites showed significantly more microleakage in each group than the filled sealant. The level of microleakage was similar in the three flowable composites. Flowable composites are not recommended as pit and fissure sealants because more microleakage can occur even when occlural fissures are mechanically widened.

벼 기계이앙 재배시 생장조절제 처리가 생육 및 도복에 미치는 영향

金正鎬,吳成煥,李哲遠,宋凡憲,朴相一 忠北大學校 農業科學硏究所 2002 農業科學硏究 Vol.19 No.-

The study was carried out to examine the effects of plant growth regulators, 2,4-D, IBP, and Inabenfide, on growth responses and lodging resistance of rice in cultivation of the machine transplanting The Chucheong cultivar of Japonica variety was used Growth responses including culm length and lodging index etc and rice yields were measured at major growth stages and harvest time Culm length was the shortest in the treatment of IBP + Inabenfide, shortening it to 106 cm compared to that of the control, showing the best application effect of the plant growth regulator The length of internode between third and fourth internodes of lower culm was shortened to 55cm with treatment of IBP + Inabenfide compared to that of the control and it between fourth and fifth internodes was shortened to 2.9 cm with treatment of Inabenfide, showing the best effectiveness 50% of lodging was reduced by treating the plant growth regulator, 45 lodging index with treatment of IBP + Inabenfide compared to 90 of the control Resulting to reducing the lodging, rice yield was increased more with treatment of IBP + Inabenfide, to about 87%, than that of the control.

다양한 함불소가스들의 고비점 용매에 관한 용해도 측정 및 엑막에 의한 분리

최평호,이상학,김철웅,김범식,김광주,이정민,박인준 한국공업화학회 2002 응용화학 Vol.6 No.2

The solubility of various fluoro-gases in high boiling-point solvents, n-Cnh_2n+_1OH(6<n<10)and ionic liquids(+ ions: 1-Buty 1-3-methy1-imidazolium, 1-Ethy1-3-methy1-imidazolium, - ions:BF_4, PF_6, S_6F_6, F_6O_4S_6) were measured at temperatures from 0 to 30℃, at total pressures up to 4 bat Solubility increased significantly with the decrease of n in n-Cn_2N+OH and showed no such a difference in the types of ionic liquids, Especially, the solubility of R22 gas in these solvents increases rapidly with increases in pressure and decreases in temperature, whereas other fluoro-gases were showed a little solubility Liquid-supported membranes were prepared with these solvents and polymer matrix and were applied to the separation of fluoro-gases including R22 gas. A high permeability and selectivity were exhibited in R22 gas against other fluoro-gases.

유기질비료 시비량에 따른 오미자 묘목의 초기 생육특성에 관한 연구

이경아,박태호,박성용,김도현,오문국,김민수,안태진,이상원,김영국,안영섭,박충범,차선우,송범헌 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.1

This study was carried out to have basic and applied informations to find the optimum nitrogen fertilization level for organic cultivation and to investigate early growth characteristics of Schizandra chinensis Baillon. Different application levels of the organic fertilizer were based on the recommended rate of N fertilizer. Five different levels of the organic fertilizer were applied to S. chinensis and early growth responses such as the length of vine, the diameter of vine, the number of leaves, and the number of branches were investigated. Lengths of main and total vines were shown longer at the level of 1.0 time organic fertilizer than those at others. The vine length at early growth was the highest at the level of 1.0 time organic fertilizer among the treatments. Stem diameter was shown thicker level of 1.0 time organic fertilizer than others on August and September, whereas on October it was the thickest in the conventional cultivation. Number of leaf was increased higher at the level of 1.0 and 2.0 times organic fertilizer than that in the compared to conventional. The number of branches was appeared the lowest at the level of 1.0 time organic fertilizer at early growth, however they were increased through progressing their growth stages. They were not significantly difference among the treatments except for the level of 2.0 times organic fertilizer. Based on the research results, the growth of S. chinensis was commonly higher at the level of 1.0 time organic fertilizer compared to other treatments.

비축대칭 평금형 압출에서 유동제어에 관한 실험적 연구-Ⅱ

김영호(Young-Ho Kim),배원병(Won-Byong Bae),강범수(Beom一Soo Kang),박재우(Jae-Woo Park) Korean Society for Precision Engineering 1997 한국정밀공학회지 Vol.14 No.3