멜론 덩굴쪼김병균(Fusarium oxysporum f. sp. melonis) race 2에 대한 멜론 유전자원 저항성 평가

황애진(Aejin Hwang),노형준(Hyungjun Noh),허온숙(Onsook Hur),노나영(Nayoung Ro),이정윤(Jung-Yoon Yi),이재은(Jae-eun Lee),김빛샘(Bichsaem Kim),김지현(Ji Hyeon Kim) 한국육종학회 2021 한국육종학회지 Vol.53 No.4

Fusarium wilt is a serious disease that causes damping-off, serious wilt symptoms, or death in melon. The aim of this study wasto evaluate the resistance of melon germplasm to Fusarium wilt and select promising disease-resistant accessions for further applications. Resistanceto Fusarium wilt was examined in 216 melon germplasms by the root-dipping inoculation method with Fusarium oxysporum f. sp. melonis fungalisolate KACC43206 (race 2). Inoculated seedlings were incubated for four weeks and assessed for disease symptoms using indices ranging fromindex 1 (no symptoms) to 5 (plant wither). A total of 34 accessions showed a disease index < 2.0. Disease indices of 10 accessions were 1.0four weeks after inoculation. The resistant accessions of melon are expected to help in breeding commercial varieties with Fusarium wilt resistance.

Maxent를 이용한 노린재 5종의 분포에 영향을 미치는 요인 분석

황애진,이준호 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.10

기후변화는 해충들의 분포 변화를 유발할 것으로 예상되며, 이를 예측하기 위해 서는 해충들의 분포에 영향을 주는 환경요인을 조사 할 필요가 있다. 본 연구에서는 Maxent(Maximum entropy)모형을 이용하여 벼를 가해하는 해충인 가시점둥글노 린재(Eysarcoris aeneus)와 호리허리노린재(Leptocorisa chinensis), 콩을 가해하 는 가로줄노린재(Piezodorus hybneri), 콩 및 과수류를 가해하는 썩덩나무노린재 (Halyomorpha halys)와 톱다리개미허리노린재(Riptortus clavatus)의 분포에 영 향을 미치는 요인을 분석하였다. 이들 노린재의 분포자료는 국내외 논문을 비롯하 여 온라인으로 공개되고 있는 농촌진흥청 국립농업과학원 곤충표본관과 국립수목 원 국가생물종지식정보시스템의 표본자료를 이용하였다. 노린재의 분포에 영향 을 줄 수 있는 변인으로는 기후 및 고도 자료를 사용하였으며, 기후 자료는 Worldclim에서 제공하는 1950~2000년 동안의 평균 기후 자료를 이용하였다. 노 린재 5종에 대한 Maxent 모형 분석 결과 노린재의 분포에 가장 큰 영향을 미치는 요 인으로 가로줄노린재는 고도(중요도 29.1%), 가시점둥글노린재는 겨울철 최저기 온(중요도 30.3%), 호리허리노린재는 기온의 연교차(중요도 37.5%)이었다. 한편, 가장 건조한 달의 강수량은 썩덩나무노린재(중요도 19.9%)와 톱다리개미허리노 린재(중요도 16.3%)에 영향을 미치는 것으로 분석되었다. 모형의 정확도를 나타내 는 AUC(Area Under the Curve)값은 0.7이상으로, 향후 기후변화에 따른 분포 변화 예측에 정밀 기상 자료를 이용한 Maxent모형을 활용할 수 있을 것으로 생각된다.

β-Carotene, Cucumisin Content and Fruit Morphology of Melon (Cucumis melo L.) Germplasm Collections

김영지(Yeong-Jee Kim),황애진(Ae-Jin Hwang),노재종(Jae-Jong Noh),왕샤오한(Xiaohan Wang),이재은(Jae-Eun Lee),유은애(Eunae Yoo),이수경(Sookyeong Lee),황소정(Sojeong Hwang),김명곤(Myung-Kon Kim),노형준(Hyungjun Noh) 한국자원식물학회 2021 한국자원식물학회지 Vol.34 No.6

Melon fruits are a year-old plant in the family Cucurbitae and are the most cultivated fruit in tropical countries. Melon flesh is an important source of anti-cancer, antioxidant effects, attracting attention as a functional food. We investigated the morphological properties, β-carotene content, and cucumisin activity of seeds of 58 melon genetic resources. Melon resources have shown various morphological properties. Melons grouped by morphological properties were the five groups. β-carotene content varied between 82.34 ㎎/㎏, 86.75 ㎎/㎏, 25.56 ㎎/㎏, 86.25 ㎎/㎏, and 54.65 ㎎/ ㎏. Between β-carotene, cucumisin activity and other quantitative fruit morphological properties, the color of the pulp and the firmness of the pulp had a significant amount of correlation between the β-carotene content. However, cucumisin activity and β-carotene content had significant negative correlation, and the color of the fruit and shape of the fruit had significant negative correlation with β-carotene content and activity of cucumisin. Our study vindicated that high diversity in melon morphological characters of genetic sources that provide beneficial baseline data for the future and in the activity of β-carotene and cucumisin, and these results could predict indicators of β-carotene content by the length of leaf, the width of fruit, the length of joint, and the length of seed, and collectively the morphological properties of melons could help predict indicators of β-carotene content and help develop functional sarcoma and farmhouse cultivation.

고추 유전자원의 매운맛 함량 변이

고호철,이수경,노나영,황애진,허온숙,김빛샘,한범수,우희종,이윤정,김성훈 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

고추약한모틀바이러스 병원형 P1,2 및 P1,2,3 생물검정을 통한 저항성 고추유전자원 선발

허온숙,곽해련,노나영,최유미,이수경,황애진,김빛샘,김성훈,한범수 한국육종학회 2022 한국육종학회지 Vol.54 No.1

Pepper mild mottle virus (PMMoV), a member of the genus Tobamovirus, is one of the most threatening pathogens affecting commercial pepper production, and it is classified into pathotypes P1,2 and P1,2,3. As chemical and physical treatments show limited success in controlling PMMoV, resistant pepper varieties are considered the most effective means of disease control. Two hundred pepper germplasms, including 167 accessions of Capsicum chinense and 5 reference accessions known as resistant L alleles, were assessed using a bioassay to select germplasms resistant to PMMoV. Six accessions, including IT261210, were resistant to both PMMoV pathotypes P1,2 and P1,2,3 as they developed necrotic local lesions only on inoculated leaves, whereas no symptoms were observed on the upper leaves. Moreover, RT-PCR results of the upper leaves of these accessions were also negative. Thus, the identified accessions may be a novel source of genetic resistance against present or emerging new pathotypes and may be useful for differentiating L alleles.

Evaluation of Different Inoculation Methods for Screening of Sclerotinia rot and Phytophthora blight in Perilla Germplasm

Tania Afroz,이호선,전영아,성정숙,이주희,Awraris Derbie Assefa,노재정,황애진,허온숙,노나영,이재은,이명철 한국작물학회 2019 Journal of crop science and biotechnology Vol.22 No.2

Sclerotinia rot and Phytophthora blight are devastating diseases of perilla that can cause huge loss of perilla production. Screening disease resistant genetic resources is necessary to develop disease-resistant cultivars and conduct related research. The objective of this study was to find suitable inoculation methods for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm. Three different inoculation methods [i.e. detached leaf (agar plug placed on start point of vein, 1/3 point of leaf vein and front side, back side), stem tip, and soil drenching] with three-growth stages (two, four & six-leaf stage) at two different temperatures (20 & 25oC) were assayed for their effectiveness for screening using IT 229019 and Sang-yeop perilla germplasm. Based on disease lesion, 20oC in two-leaf stage with detached leaf method (start point of vein) was found to be an efficient method for screening of Sclerotinia rot whereas 25oC in two-leaf stage with stem tip was an effective method for screening of Phytophthora blight in perilla germplasm. However, stem tip method was laborious as it required more time. Therefore, detached leaf method (start point of vein and back side) was a simple and rapid method that could be preferably used for screening of Sclerotinia rot and Phytophthora blight in perilla germplasm.

Screening of Sclerotinia Rot Resistant Korean Origin Perilla (Perilla frutescens) Germplasm Using a Detached Leaf Method

이호선,Tania Afroz,전영아,성정숙,이주희,Awraris Derbie Aseefa,노재정,황애진,허온숙,노나영,이재은 한국자원식물학회 2019 한국자원식물학회지 Vol.32 No.6

Sclerotinia rot, caused by Sclerotinia sclerotiorum, is a devastating disease that poses a serious threat to perilla production in Korea. Identifying effective sources of resistance offers long term prospects for improving management of this disease. Screening disease resistant genetic resources is important for development of disease-resistant, new cultivars and conduct related research. In the present study, perilla germplasm were screened in vitro against S. sclerotiorum using detached leaf method. Among 544 perilla accessions, two were highly resistant (IT226504, IT226533), five were resistant (IT226561, IT226532, IT226526, IT226441, and IT226589), five were moderately resistant (IT226525, IT226640, IT226568, IT220624, and IT178655), 16 were moderately susceptible, 31 were susceptible, and 485 were highly susceptible. The resistant accessions in this study could serve as resistance donor in the breeding of Sclerotinia rot resistance or subjected to selection procedure of varietal development for direct use by breeders, farmers, researchers, and end consumers.

Using Phenolic Compounds and Some Morphological Characters as Distinguishing Factors to Evaluate the Diversity of Perilla Genetic Resources

Awraris Derbie Assefa,전이진,이주희,이호선,허온숙,노재정,노나영,황애진,성정숙,이재은 한국자원식물학회 2020 한국자원식물학회지 Vol.33 No.1

The objectives of this study were to evaluate total phenolic content (TPC) and individual phenolic compounds in leaves of perilla genetic resources, assess whether they could be used as distinguishing factor among germplasms, and evaluate their relationship with some quantitative and qualitative morphological characters. TPC and individual phenolic compounds were determined using Folin-Ciocalteu method and UPLC-PDA system, respectively. Wide variations in TPC (7.99 to 133.70 ㎎GAE/g DE), rosmarinic acid (ND to 21.05 ㎎/g DE), caffeic acid (ND to 1.17 ㎎/g DE), apigenin- 7-O-diglucuronide (ND to 2.21 ㎎ luteolin equivalent (㎎LUE)/g DE), scutellarein-7-O-glucuronide (ND to 5.25 ㎎ LUE/g DE), and apigenin-7-O-glucuronide (ND to 2.81 ㎎ LUE/g DE) were observed. Intensities of green pigment at abaxial and adaxial leaf surfaces were positively correlated with phenolic compounds whereas leaf length and width had negative correlation. Purple pigmented accessions were shorter in leaf length and width but exhibited higher amount of phenolic compounds compared to green pigmented accessions in most cases. Leaf shape was not related with content of phenolic compounds, color of leaves, and length/width of leaves. TPC and individual phenolic compounds along with morphological characters could be useful distinguishing factors for perilla genetic resources.

Evaluation of Bioassay Methods to Assess Bacterial Soft Rot Resistance in Radish Cultivars

Tania Afroz(타니아 아프로즈),Onsook Hur(허온숙),Nayoung Ro(노나영),Jae-eun Lee(이재은),Aejin Hwang(황애진),Bichsaem Kim(김빛샘),Awraris Derbie Assefa(아와리스 데비 아세파),Ju Hee Rhee(이주희),Jung Sook Sung(성정숙),Ho-sun Lee(이호선),Bum-So 한국생명과학회 2021 생명과학회지 Vol.31 No.7

세균성 무름병 균[Pectobacterium carotovorum subsp. carotovorum (Pcc)]에 의해서 일어나는 무름병은 아시아 국가에서 재배되는 무에 있어서 심각한 질병 중의 하나로 알려져 있다. 이번 연구의 목적은 상업적으로 시판되는 무 품종의 세균성 무름병의 저항성에 대한 효율적인 생물검정법을 확립하고자 하였다. 첫째, 무 품종에 대해 세균성 무름병의 효율적인 생물검정 방법을 조사하였다. 6개의 무 품종을 다양한 조건[두 가지 온도(25℃와 30℃), 3가지 접종방법(관주, 분무, 침지), 두 발생단계(2와 4잎 단계)]으로 조사하였다. 연구 결과는 무름병균 1×106 cfu/ml 농도를 분무한 4잎 단계와 30℃에서 배양한 생물검정 방법이 무 품종에 대해 가장 효율적인 방법임을 알 수 있었다. 둘째, 41개의 무 품종에 5가지 세균(KACC 10225, KACC 10343, KACC 10421, KACC 10458, KACC 13953)을 접종하여 저항성을 조사하였다. KACC 10421가 세균성 무름병의 감수성 및 저항성 질병 정도를 가장 잘 나타냈다. 41개의 무 품종 중 13개는 무름병 균에 대해 중도 저항성을 나타냈고 28개는 감수성을 나타냈다. 이 연구에서 중도저항성 무 품종은 세균성 무름병 저항성 육종을 위한 저항성 자원으로 활용 가능하고 육종가, 농민, 연구자, 최종 소비자에 의해서 다양한 목적을 위해 사용 가능할 것으로 사료된다. Bacterial soft rot, caused by Pectobacterium carotovorum subsp. carotovorum (Pcc), is one of the destructive diseases of radish (Raphanus sativus) in Asian countries. The objective of this study was to establish an efficient bioassay method for the evaluation of bacterial soft rot resistance in commercial radish cultivars. First, an efficient bioassay method for examining resistance to bacterial soft rot in commercial radish cultivars was investigated. Six commercial radish cultivars were tested under various conditions: two temperatures (25℃ and 30℃), three inoculations methods (drenching, spraying, and root dipping), and two growth stages (two- and four-leaf stages). The results suggested that spraying with 1×106 cfu/ml of bacterial inoculums during the four-leaf stage and incubating at 30℃ could be the most efficient screening method for bacterial soft rot resistance in commercial radish cultivars. Second, we investigated the degree of resistance of 41 commercial radish cultivars to five Pcc isolates, namely KACC 10225, KACC 10343, KACC 10421, KACC 10458, and KACC 13953. KACC 10421 had the strongest susceptibility in terms of moderately resistant disease response to bacterial soft rot. Out of the 41 radish cultivars, 13 were moderately resistant to this pathogen, whereas 28 were susceptible. The moderately resistant radish cultivars in this investigation could serve as resistance donors in the breeding of soft rot resistance or could be used to determine varietal improvement for direct use by breeders, scientists, farmers, researchers, and end customers.

고추약한모틀바이러스 병원형 P1,2 및 P1,2,3 생물검정을 통한 저항성 고추유전자원 선발

허온숙(On-Sook Hur),곽해련(Hae-Ryun Kwak),노나영(Na-Young Ro),Choi(Yumi Choi),이수경(Sukyeong Lee),황애진(Aejin Hwang),김빛샘(Bichsaem Kim),김성훈(Seong-Hoon Kim),한범수(Bum-Soo Hahn) 한국육종학회 2022 한국육종학회지 Vol.54 No.2

Pepper mild mottle virus (PMMoV), a member of the genus Tobamovirus, is one of the most threatening pathogens affecting commercial pepper production, and it is classified into pathotypes P1,2 and P1,2,3. As chemical and physical treatments show limited success in controlling PMMoV, resistant pepper varieties are considered the most effective means of disease control. Two hundred pepper germplasms, including 167 accessions of Capsicum chinense and 5 reference accessions known as resistant L alleles, were assessed using a bioassay to select germplasms resistant to PMMoV. Six accessions, including IT261210, were resistant to both PMMoV pathotypes P1,2 and P1,2,3 as they developed necrotic local lesions only on inoculated leaves, whereas no symptoms were observed on the upper leaves. Moreover, RT-PCR results of the upper leaves of these accessions were also negative. Thus, the identified accessions may be a novel source of genetic resistance against present or emerging new pathotypes and may be useful for differentiating L alleles.