가족성 암 증후군이 의심되는 환자에서 유전 상담의 영향: TP53 유전자 돌연변이가 확인된 Li-Fraumeni 유사 증후군 환자를 통한 증례 보고

황상미,이은숙,신상훈,공선영 대한진단검사의학회 2008 Annals of Laboratory Medicine Vol.28 No.6

We report a 26-yr-old female patient with bilateral breast cancer who was clinically diagnosed with Li-Fraumeni like syndrome (LFL) and subsequently found to have a germline mutation of the TP53 gene. The patient was initially diagnosed with right breast cancer at age 24 yr and then with left breast cancer at age 25 yr. Surgery and radiotherapy were performed accordingly. The patient had a family history of various types of early onset cancers and was referred to a genetic counseling clinic. She was clinically diagnosed with LFL. Genetic analysis of the TP53 tumor suppressor gene was performed with the patient’s consent. Direct sequencing of TP53 gene exons 5, 6, 8, 9, and 11 revealed a germline missense mutation, resulting in an amino acid change from an arginine to a histidine (g.13203G>A, p.R175H). Considering the family history, individualized cancer surveillance was performed including a gastroscopy and a brain MRI. Even though the patient had not shown any neurological symptoms, a huge mass on the temporal lobe was incidentally found and the patient received surgery and radiotherapy. Although the residual mass required further treatment, the patient decided on supportive care alone and was discharged. We report a case of LFL, with a germline TP53 mutation, which was confirmed by gene sequencing in Korea. This case shows how genetic predisposition screening and counseling in patients, suspected of having a familial cancer syndrome, can influence the course of the patient. (Korean J Lab Med 2008;28:493-7)

부모의 성취압력과 고등학생의 시험불안의 관계에서 학업적 자기효능감과 자아존중감의 조절효과

황상미,정현희 한국청소년학회 2012 청소년학연구 Vol.19 No.5

본 연구는 부모의 성취압력과 고등학생의 시험불안 간의 관계에서 학업적 자기효능감과 자아존중감의 조절효과를 검증하고자 하였다. 본 연구를 위해 D광역시에 소재하는 S고등학교 1, 2학년 남녀 학생 410명을 대상으로 부모의 성취압력, 시험불안, 학업적 자기효능감, 자아존중감을 측정하였다. 부모의 성취압력과 고등학생의 시험불안 간의 관계에서 학업적 자기효능감과 자아존중감의 조절효과를 알아보기 위하여 위계적 중다회귀분석을 실시하였다. 연구의 결과는 다음과 같았다. 첫째, 학업적 자기효능감은 부모의 성취압력과 고등학생의 시험불안 간의 관계에서 조절효과가 있는 것으로 나타났다. 학업적 자기효능감 하위요인의 경우, 과제난이도선호, 자기조절효능감, 자신감 모두 부모의 성취압력이 고등학생의 시험불안에 미치는 부정적인 영향력을 조절하는 것으로 나타났다. 둘째, 자아존중감은 부모의 성취압력과 고등학생의 시험불안 간의 관계에서 조절효과가 있는 것으로 나타났다. 이와 같은 연구결과는 학교 현장에서 부모의 성취압력으로 인한 고등학생의 시험불안을 예방하고 조절하기 위하여 학업적 자기효능감과 자아존중감을 향상시키는데 관심과 노력이 필요함을 시사해준다. The purpose of this study was to examine the moderating effects of academic self-efficacy and self-esteem on the relation between parental achievement pressure and high school students' test anxiety. Participants of the study were 410 first and second year high school students in D city. Multiple regression analysis was conducted to examine the moderating effects of academic self-efficacy and self-esteem on the relation between parental achievement pressure and high school students' test anxiety. The major findings of the study were as follows: First, academic self-efficacy had a significant moderating effect on the relation between parental achievement pressure and high school students' test anxiety. All sub-factors moderated the negative effects of parental achievement pressure on high school students' test anxiety. Second, self-esteem also showed a significant moderating effect on the relation between parental achievement pressure and high school students' test anxiety. These results suggest that efforts are needed to improve students' academic self-efficacy and self-esteem order to prevent their test anxiety caused by parental achievement pressure.

‘한 학기 한 권 읽기’를 위한 도서 선정 사례 - 북매치를 중심으로

황상미 광주교육대학교 초등국어연구소 2020 초등국어과교육 Vol.27 No.-

이 연구의 목적은 ‘한 학기 한 권 읽기’에서 도서선정의 중요성을 인식하고 북매치 전략을 활용하여 초등학교 3,4학년 도서를 선정하는 것이다. 이와 같은 연구의 목적을 달성하기 위해 권장도서 목록을 발행하는 공신력 있는 기관 두 곳을 선정하여 54권을 분석 대상 도서로 선정하였다. 54권의 도서를 분석하기 위해 북매치 전략을 기준으로 평정 하였으며, 결과의 신뢰성을 위해 9명의 교사와 함께 분석·검토하였다. 권장도서들을 살펴 본 결과 첫째, 이야기가 있는 서사 문학 분야의 책이 높은 평가를 받았다. 둘째, 과학·예술 등 전문 지식과 정보를 전달하는 책은 비교적 낮은 평가를 받았다. 셋째, 책 선택 전략을 활용한 결과 도서선정의 어려움이 낮아졌다. ‘한 학기 한 권 읽기’에서 도서선정의 중요성을 인식하면서도 이에 대한 연구는 부족하다. 이를 바탕으로 교사의 도서선정 연구가 활발해지길 기대한다.

The application of an in situ karyotyping technique for mesenchymal stromal cells: a validation and comparison study with classical G-banding

황상미,Cha-ja See,최정은,김선영,김정아,최규태,권지석,박시내,임경옥,오일환,이동순 생화학분자생물학회 2013 Experimental and molecular medicine Vol.45 No.12

The cytogenetic analysis of mesenchymal stromal cells (MSCs) is essential for verifying the safety and stability of MSCs. An in situ technique, which uses cells grown on coverslips for karyotyping and minimizes cell manipulation, is the standard protocol for the chromosome analysis of amniotic fluids. Therefore, we applied the in situ karyotyping technique in MSCs and compared the quality of metaphases and karyotyping results with classical G-banding and chromosomal abnormalities with fluorescence in situ hybridization (FISH). Human adipose- and umbilical cord-derived MSC cell lines (American Type Culture Collection PCS-500-011, PCS-500-010) were used for evaluation. The quality of metaphases was assessed by analyzing the chromosome numbers in each metaphase, the overlaps of chromosomes and the mean length of chromosome 1. FISH was performed in the interphase nuclei of MSCs for 6q, 7q and 17q abnormalities and for the enumeration of chromosomes via oligo-FISH in adipose-derived MSCs. The number of chromosomes in each metaphase was more variable in classical G-banding. The overlap of chromosomes and the mean length of chromosome 1 as observed via in situ karyotyping were comparable to those of classical G-banding (P¼0.218 and 0.674, respectively). Classical G-banding and in situ karyotyping by two personnel showed normal karyotypes for both cell lines in five passages. No numerical or structural chromosomal abnormalities were found by the interphase-FISH. In situ karyotyping showed equivalent karyotype results, and the quality of the metaphases was not inferior to classical G-banding. Thus, in situ karyotyping with minimized cell manipulation and the use of less cells would be useful for karyotyping MSCs.

실외 전래놀이 활동이 유아의 신체적 자아개념과 또래유능성에 미치는 영향

황상미,김미애 한국어린이미디어학회 2019 한국어린이미디어학회 학술대회 자료집 Vol.2019 No.12

본 연구는 실외 전래놀이 활동을 통하여 유아의 신체적 자아개념과 또래유능성에 미치는 영향을 분석하는데 목적을 둔 양적연구이다. 연구대상은 S시에 소재한 공립 단설 A유치원 O반과 △반 만5세 원아 40명(실험집단 20명, 비교집단 20명)을 대상으로 하였다. 연구기간은 2019년 4월부터 2019년 7월의 기간 동안 실시하였으며, 사전검사-실험처치-사후검사 순으로 이루어졌다. 실험집단은 한 주에 2회 실외 전래놀이 활동을 처치하였으며, 비교집단은 한 주 1회만 실내 전래놀이를 접해 본 후 일반적인 바깥놀이 활동을 실시하였다. 실험처치의 영향을 분석하기 위해서 SPSS/for window 21.0프로그램을 사용하여 사전·사후 검사에 대한 실험집단과 비교집단의 평균과 표준편차를 산출하였으며, 공변량 분석(ANCOVA)를 실시하였다. 분석 결과 실외 전래놀이 활동이 유아의 신체적 자아개념과각 하위영역 및 또래유능성과 각 하위영역에서 긍정적인 영향을 미쳤다는 결과를 얻을 수 있었다.

Classification of acute myeloid leukemia

황상미 대한혈액학회 2020 Blood Research Vol.55 No.-

The World Health Organization (WHO) Classification of Tumors of Hematopoietic and Lymphoid Tissues was revised in 2017 on the basis of recent high-throughput sequencing and gene expression data on hematologic malignancies. This review explores the current WHO classification of acute myeloid leukemia (AML) and related precursor neoplasms, highlighting the changes made in the current edition and focusing on the diagnosis of AML.

Comparison of Ion Personal Genome Machine Platforms for the Detection of Variants in BRCA1 and BRCA2

황상미,이기찬,이민섭,박경운 대한암학회 2018 Cancer Research and Treatment Vol.50 No.1

Purpose Transition to next generation sequencing (NGS) for BRCA1/BRCA2 analysis in clinical laboratories is ongoing but different platforms and/or data analysis pipelines give different results resulting in difficulties in implementation. We have evaluated the Ion Personal Genome Machine (PGM) Platforms (Ion PGM, Ion PGM Dx, Thermo Fisher Scientific) for the analysis of BRCA1/2. Materials and Methods The results of Ion PGM with OTG-snpcaller, a pipeline based on Torrent mapping alignment program and Genome Analysis Toolkit, from 75 clinical samples and 14 reference DNA samples were compared with Sanger sequencing for BRCA1/BRCA2. Ten clinical samples and 14 reference DNA samples were additionally sequenced by Ion PGM Dx with Torrent Suite. Results Fifty types of variants including 18 pathogenic or variants of unknown significance were identified from 75 clinical samples and known variants of the reference samples were confirmed by Sanger sequencing and/or NGS. One false-negative results were present for Ion PGM/OTG-snpcaller for an indel variant misidentified as a single nucleotide variant. However, eight discordant results were present for Ion PGM Dx/Torrent Suite with both falsepositive and -negative results. A 40-bp deletion, a 4-bp deletion and a 1-bp deletion variant was not called and a false-positive deletion was identified. Four other variants were misidentified as another variant. Conclusion Ion PGM/OTG-snpcaller showed acceptable performance with good concordance with Sanger sequencing. However, Ion PGM Dx/Torrent Suite showed many discrepant results not suitable for use in a clinical laboratory, requiring further optimization of the data analysis for calling variants.

실버에이지를 위한 인쇄물과 웹사이트에 나타난 정보디자인 방법에 관한 연구

황상미(Hwang Sang-Mi),백진경(Paik Jin-Kyung),최인규(Choi In-Kyu) 한국디자인학회 2002 디자인학연구 Vol.- No.46

moire

황상미 ( Hwang Sang Mi ) 한국기초조형학회 2018 한국기초조형학회 작품집 Vol.2018 No.2

실험적 잡지(스트리트매거진, 또는 인쇄매체의 실험적 잡지) 디자인의 인터넷에서의 표현방법에 관한 연구

황상미(Hwang Sang Mi),최인규(Choi In Kyu) 한국디자인학회 2002 디자인학연구 Vol.- No.50