적콜라비 새싹채소 종자에서 분리한 Escherichia coli strain RC-4-D의 생장예측모델

최수연,류상돈,박병용,김세리,김현주,이승돈,김원일,Choi, Soo Yeon,Ryu, Sang Don,Park, Byeong-Yong,Kim, Se-Ri,Kim, Hyun-Ju,Lee, Seungdon,Kim, Won-Il 한국식품저장유통학회 2017 한국식품저장유통학회지 Vol.24 No.6

본 연구는 시중 유통되고 있는 새싹채소 재배용 적콜라비 종자에서 분리한 E. coli strain RC-4-D의 생장예측모델을 개발하기 위해 수행되었다. 각 온도조건(10, 15, 20, 25, $30^{\circ}C$) 별로 적콜라비 중 E. coli strain RC-4-D 밀도 변화를 조사하였고 Baranyi model을 1차 생장예측모델로 이용하였고 각 온도별로 최대생장률(${\mu}max$)과 $10^{\circ}C$를 제외한 유도기(LPD) 값을 도출하였다. E. coli strain RC-4-D의 최대생장률에 대한 2차 생장예측모델로써 suboptimal Ratkowsky square-root, suboptimal Huang square-root, suboptimal Arrhenius-type 세 종류의 모델을 비교하였다. 모델 적합성 검정 결과, suboptimal Huang square-root 모델이 정확도가 가장 높고 suboptimal Ratkowsky square-root 모델이 편차가 가장 적은 것으로 나타났다. 종합적으로, RMSE가 0.100, $A_f$가 1.255, $B_f$가 0.999인 suboptimal Ratkowsky square-root 모델이 온도의 영향을 설명하는 가장 적합한 2차 생장예측 모델인 것으로 나타났다. 본 연구에서 개발한 모델은 적콜라비 새싹채소 생산에 있어서 E. coli의 생장을 예측하고 미생물 위해성평가를 수행하는데 활용될 것으로 기대된다. This study was conducted to develop a predictive model for the growth of Escherichia coli strain RC-4-D isolated from red kohlrabi sprout seeds. We collected E. coli kinetic growth data during red kohlrabi seed sprouting under isothermal conditions (10, 15, 20, 25, and $30^{\circ}C$). Baranyi model was used as a primary order model for growth data. The maximum growth rate (${\mu}max$) and lag-phase duration (LPD) for each temperature (except for $10^{\circ}C$ LPD) were determined. Three kinds of secondary models (suboptimal Ratkowsky square-root, Huang model, and Arrhenius-type model) were compared to elucidate the influence of temperature on E. coli growth rate. The model performance measures for three secondary models showed that the suboptimal Huang square-root model was more suitable in the accuracy (1.223) and the suboptimal Ratkowsky square-root model was less in the bias (0.999), respectively. Among three secondary order model used in this study, the suboptimal Ratkowsky square-root model showed best fit for the secondary model for describing the effect of temperature. This model can be utilized to predict E. coli behavior in red kohlrabi sprout production and to conduct microbial risk assessments.

아주대학교병원 소아치과에서 시행된 전신마취 하 치과치료에 관한 실태조사

최수연,공은경,백광우,Choi, Soo-Yeon,Kong, Eun-Kyung,Baek, Kwang-Woo 대한치과마취과학회 2014 Journal of Dental Anesthesia and Pain Medicine Vol.14 No.4

Background: General anesthesia is a necessary method for successful dental treatment for children, compromised patients and the disabled who have difficulty in cooperation. The aim of this study was to assess dental treatment under general anesthesia at the department of pediatric dentistry, Ajou university hospital on children and the disabled. Methods: 217 general anesthesia from June 2010 to June 2014 were assessed for this study. Patient's distribution, treatment pattern, reasons for general anesthesia, distribution of combined operation, duration of anesthesia, treatment, frequency of general anesthesia and agents for general anesthesia were examined. Results: The proportion of male, the disabled were higher and above 19 years age group was the highest. Combined operation with otolaryngology was highest. Main reasons for general anesthesia were mental retardation for the disabled and uncooperative behavior for the non-disabled. Percentage of restorative treatment was the highest. Average anesthesia duration was 186 minutes and average treatment time was 143 minutes. Most of the airway was maintained by nasotracheal intubation and induction was done by sevoflurane. Conclusions: General anesthesia for dental treatment has been increasing for children, the disabled who have a difficulty of cooperation. The demand for dental treatment under general anesthesia is expected to continuously increase. Therefore, continuous research and studies should be done to establish efficiency and safety of general anesthesia and provide an enhanced environment for treatment.

국내 토양으로 분리된 Bacillus thuriniensis subsp. kurstaki CAB133균주의 생물학적 특성

최수연,조민수,김태환,김진수,백승경,윤영남,홍순성,유용만,Choi, Su-Yeon,Cho, Min-Su,Kim, Tae-Hwan,Kim, Jin-Su,Pack, Seung-Kyung,Youn, Young-Nam,Hong, Soon-Sung,Yu, Yong-Man 한국응용곤충학회 2008 한국응용곤충학회지 Vol.47 No.2

국내의 난방제 해충에 선택적으로 생물활성을 나타내는 균주를 선발하기 위하여 작물재배 지역의 토양으로부터 채취한 115개의 토양샘플 중 46개의 Bacillus thuringiensis 균주를 분리하였다. 이러한 B. thuringiensis균주를 사용하여 난방제 농업해충에 생물활성을 검정한 결과 배추좀나방(Plutella xylostella)에 CAB119을 포함한 35균주, 파밤나방(Spodoptera litura)에는 CAB128, CAB141균주가, 담배거세미나방(Spodoptera exigua)은 CAB133과 CAB159균주가 효과를 나타냈으며 CAB162균주는 3종류의 모든 해충에 높은 활성을 보였다. 분리된 B. thuringiensis CAB133 균주는 H serotype에 의한 혈청학적 동정과 SDS-PAGE를 통한 독소 단백질 패턴에서 B. thuringiensis subsp. kurstaki (3abc)로 동정되었다. 담배거세미나방 2령 유충에 대한 활성검정의 결과 B. thuringiensis subsp. kurstaki (3abc) CAB133, CAB162의 두 균주에 대한 $LD_{50}$ 값은 각각 0.089, $3.144{\mu}g/ml$로 높은 활성을 나타났다. 담배거세미나방의 령기에 따른 생물실험에서 CAB133 균주 $1.5{\times}10^6(cfu/ml)$에서 1령의 경우 3일, 2령은 5일에 100%의 사충율을 보였다. 담배거세미나방의 경우 B. thuringiensis를 섭식 후 먹기를 중단하여 성장이 멈추면서 $5{\times}7$일 후에 사망하였다. 그러므로 B. thuringiensis의 결정성독소단백질$(1.267{\mu}g/ml)$를 섭식하고 성장하지 못하는 담배거세미나방 유충의 무게는 대조군보다 5일후 약 30배정도 차이로 나타나서 사망하였다. To screen highly active Bacillus thuringiensis isolates against Spodoptera litura (Lepidoptera, Noctuidae), 46 B. thuringiensis was isolated from 115 samples obtained from several crop soils. Especially, B. thuringiensis subsp. kurstaki CAB133 and CAB162 isolates showed 100% mortality against S. litura. $LD_{50}$ values of CAB 133, CAB162 and HD-1 strains of B. thuringiensis subsp. kurstaki were 0.089, 3.144 and $0.513{\mu}g/ml$ against 2nd larva of S. litura, respectively. The weight of 3rd larva of S. litura which were fed crystal inclusion protein $(1.267{\mu}g/ml)$ with B. thuringiensis subsp. kurstaki CAB133 was about 30 times lass than control group. CAB133 and CAB 162 strains of B. thuringiensis subsp. kurstaki which were taken a highly toxity against S. litura were analyzed by SDS-PAGE, and estimated the molecular weight of the Cry proteins. Their serological identification by H serotypes were showed B. thuringiensis subsp. kurstaki (3abc) type.

AI 반응 AR명함의 Platform 설계 및 Source Coding

최수연,한수연,박대우,Choi, Su-Youn,Han, Su-Yeon,Park, Dea-Woo 한국정보통신학회 2020 한국정보통신학회논문지 Vol.24 No.4

4차 산업혁명의 기술 발달은 세계와 사회의 발전을 가속화 시키고 있다. 4차 산업기술은 Cyber World와 연결되어 국가와 사회 인프라를 발전시키고 4차 산업기술의 응용은 Cyber-city와 Cyber-life system을 창조할 수 있다. Cyber 세계와 현실 세계를 연결시키는 ICT 비즈니스 업계에서는 Cyber 업무와 현실의 업무를 연결시켜야 한다. Cyber 업무와 현실의 업무의 연결 방법 중 한 가지로 AR명함을 사용하는 것이다. AR명함을 스마트폰으로 인식하면 3D 캐릭터가 등장하여 명함 주인공을 소개하도록 한다. 또한, AR 명함은 사업자의 상품·제품·서비스가 AR로 반영되어 제품, 서비스 등에 대한 홍보가 가능하도록 한다. 연결된 유무선 5G 통신을 이용하면, 실시간 전자상거래도 가능하다. 본 논문에서는 Source Coding으로 AR명함 제작하고 AR 명함 platform을 설계할 것이다. The technological development of the Fourth Industrial Revolution is accelerating the development of the world and society. Fourth industrial technology is connected to Cyber World to develop national and social infrastructure. The application of the fourth industrial technology can create Cyber-city and Cyber-life systems. In the ICT business industry that connects Cyber with the real world, we need to connect Cyber with real-life work. It is to use AR business cards as one of the ways to connect Cyber business with real business. When an AR business card is recognized as a smartphone, a 3D character appears to introduce the main character of the business card. In addition, AR business cards are reflected in AR for product and service of business operators, enabling promotion of products, services, etc. Using connected wired and wireless 5G communication, real-time e-commerce is also possible. In this paper, AR business card will be produced with Source Coding and AR business card platform will be designed.

하이브리드 차량용 견인전동기의 절연성능 비교분석

최수연,박찬용,김성욱,박대원,길경석,이강원,Choi, Su-Yeon,Park, Chan-Yong,Kim, Sung-Wook,Park, Dae-Won,Kil, Gyung-Suk,Lee, Kang-Won 한국전기전자재료학회 2008 전기전자재료 Vol.21 No.12

The object of this paper is to acquire the data related to insulation evaluation of hybrid vehicle traction motors. We made a comparative analysis on Insulation Resistance (IR), Dielectric Absorption Ratio (DAR), and Polarization Index (PI) of the motor stators. The experiment was carried out according to IEEE Std. 43 and IEC 60085-1 for insulation resistance test standard of rotating machinery. Test voltage of 500 V was applied between a phase and the enclosure. The IR and the DAR of used motors were lower than those of new ones. The DAR and the PI were $0.92{\sim}1.02$ and $0.74{\sim}1.1$, respectively and the result did not meet the recommendation basis 2 for insulation level H. From the experimental results, we could prepare parameters and basis for insulation evaluation of the traction motor stator by the comparative analysis of short-time insulation resistance changes, DAR and PI.

하이브리드 차량용 견인전동기의 절연성능 비교분석

최수연,박찬용,김성욱,박대원,길경석,이강원,Choi, Su-Yeon,Park, Chan-Yong,Kim, Sung-Wook,Park, Dae-Won,Kil, Gyung-Suk,Lee, Kang-Won 한국전기전자재료학회 2008 전기전자재료학회논문지 Vol.21 No.12

The object of this paper is to acquire the data related to insulation evaluation of hybrid vehicle traction motors. We made a comparative analysis on Insulation Resistance (IR), Dielectric Absorption Ratio (DAR), and Polarization Index (PI) of the motor stators. The experiment was carried out according to IEEE Std. 43 and IEC 60085-1 for insulation resistance test standard of rotating machinery. Test voltage of 500 V was applied between a phase and the enclosure. The IR and the DAR of used motors were lower than those of new ones. The DAR and the PI were $0.92{\sim}1.02$ and $0.74{\sim}1.1$, respectively and the result did not meet the recommendation basis 2 for insulation level H. From the experimental results, we could prepare parameters and basis for insulation evaluation of the traction motor stator by the comparative analysis of short-time insulation resistance changes, DAR and PI.

지하집모기 (Culex pipiens molestus)의 방제를 위한 환경친화적 살충제의 생물검정

최수연,오세찬,조민수,백승경,김진수,김다아,길미라,윤영남,유용만,Choi, Su-Yeon,Oh, Se-Chan,Cho, Min-Su,Paek, Seung-Kyoung,Kim, Jin-Su,Kim, Da-A,Gil, Mi-Ra,Youn, Young-Nam,Yu, Yong-Man 한국응용곤충학회 2007 한국응용곤충학회지 Vol.46 No.2

몇 가지 환경 친화적 살충제를 사용하여 지하집모기 유충에 대한 생물활성을 조사하였다. 본 실험에 사용한 약제로는 키틴합성저해제인 Novaluron, 탈피촉진제인 Methoxyfenozide, 탈피억제제인 Pyriproxyfen 그리고 지질생합성저해제인 Spiromesifen을 사용하였다. 지하집모기의 유충에 대한 약제별 반수치사농도는 각각 0.00039, 0.07193, 0.65006 그리고 0.04839 ppm으로 키틴합성저해제인 Novaluron 가 가장 낮은 농도를 보였다. 모기 유충방제에 필요한 효과적인 약제 처리시기를 결정하기 위하여 모기 유충의 령기에 따른 약제 감수성 실험을 실시한 결과, Novaluron 은 부화 후 2 일차, 4 일차, 7 일차 및 10 일차에서 각각 100%, 84.5%, 71% 그리고 48.5% 의 방제가로 IGR 약제의 특정이 나타났다. 또한, Methoxyfenozide, Pyriproxyfen 그리고 Spiromesifen은 부화 후 2 일차에서 80% 이하의 살충효과로 Novaluron 과 비교하여 낮은 방제가를 나타냈었다. 한편 모기유충에 대한 효과적인 약제 노출시간을 알아본 결과 Novaluron은 100% 살충효과를 나타내는데 3시간이 필요하였고, 나머지 약제들은 12 시간 이상의 노출시간에서도 100% 방제효과를 나타내지 못하였다. Bioassay of mosquito, Culex pipiens molestus, larva was investigated by several environment-friendly insecticides. These insecticides were Novaluron as chitin synthesis inhibitor, Metho-xyfenozide as ecdysone agonist, Pyriproxyfen as juvenile hormone mimic, and Spiromesifen as lipid biosynthesis inhibitor. The 50% lethal concentration $(LC_{50})$ of these insecticides were 0.00039, 0.07193, 0.65006 and 0.04839 ppm, respectively. Novaluron has lower concentration than any other insecticide. To determine the treatment time against larval stages, insecticides were applied to different larval stages of C. pipiens molestus. Mortality ratios of mosquito larva treated with Novaluron were 100.0, 84.5, 71.0 and 48.5% on 2, 4, 7 and 10 days after hatching from eggs, respectively. Otherwise, with the other insecticides, mortality ratios were under 80% with 2 days old larva. When exposure periods were tested to 3 or 4 days old larva against 4 insecticides, at least 3 hours were needed to 100% control effect against Novaluron, and over 12 hours with other insecticides.

심혈관계 위험인자로서의 비알코올지방간질환 : 경동맥 동맥경화와의 상관관계 Relation of non-alcoholic fatty Liver disease to carotid atherosclerosis

최수연 ( Su Yeon Choi ),김동희 ( Dong Hee Kim ),강진화 ( Jin Hwa Kang ),박민정 ( Min Jung Park ),김영선 ( Young Sun Kim ),임선희 ( Seon Hee Lim ),김충현 ( Chung Hyeon Kim ),이효석 ( Hyo Suk Lee ) 대한간학회 2008 Clinical and Molecular Hepatology(대한간학회지) Vol.14 No.1

성장소설로서의 「헛간방화」: 애브너 스노프스의 역할 다시 생각하기

최수연(Soo Yeon Choi) 경성대학교 인문과학연구소 2001 인문학논총 Vol.3 No.-

우산고로쇠의 항산화 및 신경세포에서의 산화적 스트레스 개선 효과

최수연 ( Soo Yeon Choi ),김지현 ( Ji Hyun Kim ),이재민 ( Jaemin Lee ),이상현 ( Sanghyun Lee ),조은주 ( Eun Ju Cho ) 한국응용생명화학회 2017 Journal of Applied Biological Chemistry (J. Appl. Vol.60 No.2

본 연구에서는 우산고로쇠(Acer okamotoaum) methanol (MeOH)추출물과 n-BuOH, ethyl acetate (EtOAc), methylene chloride및 n-hexane의 4종 분획물을 이용하여 free radical 소거능과 총 페놀, 플라보노이드 함량을 통한 항산화 효과를 측정하였으며, 신경교세포인 C6 glial cell을 이용하여 amyloid beta_25-35 (Aβ_25-35)에 의해 유도된 산화적 스트레스에서 신경세포 보호 효과에 대해 알아보았다. 그 결과 우산고로쇠 MeOH 추출물과 4가지 유기용매 추출 분획물은 우수한 1,1-diphenyl-2-picrylhydrazylradical 소거능을 나타내었으며, 특히 EtOAc 분획물은 4.47 μg/mL의 EC₅₀ 값을 나타내 가장 우수한 소거능을 가지고 있음을 확인할 수 있었다. Superoxide radical 소거능에서도 MeOH 추출물과 분획물은 높은 소거능을 보였으며, EtOAc 분획물은 100μg/mL 농도에서 84.60%로 가장 높은 소거능을 나타내었다. 총 페놀과 플라보노이드 함량에서도 EtOAc 분획물은 다른 추출물과 분획물에 비해 월등히 높은 함량을 가지는 것으로 확인 되었다. 또한 Aβ_25-35에 의해 유발된 산화적 스트레스에서 우산고로쇠 MeOH 추출물과 4가지 유기용매 추출 분획물은 세포 생존율을 증가시켰으며, reactive oxygen species 생성을 감소 시키는 것으로 확인되었고 EtOAc 분획물이 가장 뛰어난 효과를 나타내었다. 본 연구 결과를 통해 우산고로쇠 MeOH 추출물과 4가지 유기용매 추출 분획물, 특히 EtOAc 분획물은 우수한 항산화 효과와 산화적 스트레스의 개선 효과를 가져 신경세포 보호에 효과가 있는 것으로 확인되었다. Radical scavenging effect and protective activity against oxidative stress of Acer okamotoanum were investigated. A. okamotoanum was extracted with methanol (MeOH) and then fractionated with n-BuOH, ethyl acetate (EtOAc), methylene chloride and n-hexane fractions. The MeOH extract and fractions showed strong 1,1-diphenyl-2-picrylhydrazyl and superoxide radical scavenging activity. Among the MeOH extract and fractions, the EtOAc fraction showed the strongest radical scavenging activity. In addition, total phenolic and flavonoid contents of EtOAc fraction was higher than other extract and fractions. Furthermore, we investigated the neuroprotective effect of the MeOH extract and fractions from A. okamotoanum against oxidative stress under cellular system using C6 glial cell. The C6 glial cells showed a decrease in cell viability and high production of reactive oxygen species (ROS) by the treatment of amyloid beta_25-35 (Aβ_25-35). However, with the treatment of the MeOH extract and fractions, it significantly increased the cell viability and inhibited the overproduction of ROS by Aβ_25-35. In particular, the EtOAc fraction led to significantly increase the cell viability and decrease the generation of ROS against oxidative stress by Aβ_25-35. The current study indicated that A. okamotoanum demonstrated antioxidative and neuroprotective effects. In particular, the EtOAc fraction which attributed a strong protective activity against oxidative stress.