구기자나무(Lycium chinense Mill.) 유식물체의 자엽하배축으로부터 고효율의 부정아 형성과 식물체 재생

조만현,함인기,이봉춘,김재훈,이원석,권석윤,이행순,곽상수,Jo, Man-Hyun,Ham, In-Ki,Lee, Bong-Chun,Kim, Jae-Whune,Lee, Won-Seok,Kwon, Suk-Yoon,Lee, Haeng-Soon,Kwak, Sang-Soo 한국식물생명공학회 2004 식물생명공학회지 Vol.31 No.3

두 품종 (청양재래, 명안)의 구기자나무 (Lycium chinense Mill.) 유식물체를 자엽하배축의 자엽 일부분만 남기고 절단 후, 절단면이 1.0 mg/L zeatin이 첨가된 MS배지에 접하도록 하여 암소에서 3주 배양한 후 명소로 옮겨 배양하였을 때 자엽하배축 절단면으로부터 부정아가 고빈도 (80% 이상)로 유도되었다. 그러나 자엽하배축절편을 암소에서 전 배양하지 않고, 명소에서만 배양 하였을 경우 부정아는 유도되지 않았다. 자엽하배축 절단면에서 유도된 부정아를 분리하여 1.0 mg/L IAA가 함유된 발근배지에서 4주간 배양하여 뿌리를 유도하였다. 잎과 뿌리가 잘 발달한 소식물체는 화분에 옮겨 키웠을 때 정상적인 형태의 재분화 식물체로 성장하였다. To establish high frequency shoot formation from two cultivars (Cheongyangjaerae and Myungan) of boxthorn (Lycium chinense Mill.), hypocotyl segments with cotyledons from seedlings were used as explants. High frequency adventitious shoot formation (more than 80%) were obtained from hypocotyl segments with cotyledon on MS medium supplemented with 1.0 mg/L zeatin, when precultured for 3 weeks under dark conditions followed by transfer to light conditions. But there was no shoot induction in the explants cultured without preculture under dark conditions. Roots were induced from the shoots when transferred to rooting medium supplemented with 1.0 mg/L IAA for 4 weeks. Regenerated plantlets were grown to normal mature plants in soil.

구기자나무 (Lycium chinense Mill.) 유식물체의 자엽하배축으로부터 고효율의 부정아 형성과 식물체 재생

조만현,이봉춘,권석윤,이행순,김재훈,곽상수,이원석,함인기 한국식물생명공학회 2004 JOURNAL OF PLANT BIOTECHNOLOGY Vol.31 No.3

To establish high frequency shoot formation from two cultivars (Cheongyangjaerae and Myungan) of boxthorn (Lycium chinense Mill.), hypocotyl segments with cotyledons from seedlings were used as explants. High frequency adventitious shoot formation (more than 80%) were obtained from hypocotyl segments with cotyledon on MS medium supplemented with 1.0 mg/L zeatin, when precultured for 3 weeks under dark conditions followed by transfer to light conditions. But there was no shoot induction in the explants cultured without preculture under dark conditions. Roots were induced from the shoots when transferred to rooting medium supplemented with 1.0 mg/L IAA for 4 weeks. Regenerated plantlets were grown to normal mature plants in soil. 두 품종 (청양재래, 명안)의 구기자나무 (Lycium chinense Mill.) 유식물체를 자엽하배축의 자엽 일부분만 남기고 절단 후, 절단면이 1.0 mg/L zeatin이 첨가된 MS배지에 접하도록 하여 암소에서 3주 배양한 후 명소로 옮겨 배양하였을 때 자엽하배축 절단면으로부터 부정아가 고빈도 (80% 이상)로 유도되었다. 그러나 자엽하배축절편을 암소에서 전 배양하지 않고, 명소에서만 배양 하였을 경우 부정아는 유도되지 않았다. 자엽하배축 절단면에서 유도된 부정아를 분리하여 1.0 mg/L IAA가 함유된 발근배지에서 4주간 배양하여 뿌리를 유도하였다. 잎과 뿌리가 잘 발달한 소식물체는 화분에 옮겨 키웠을 때 정상적인 형태의 재분화 식물체로 성장하였다.

Components in Chinese Cabbage (Brassica rapa ssp. campestris) as Affected by Soil pH: 6.9 vs. 7.6

조만현,함인기,이은모,이조은,강남,임용표,안길환 충남대학교 농업과학연구소 2010 농업과학연구 Vol.37 No.1

Functional and nutritional components of 7 cultivars of Chinese cabbage (CC; Brassica rapa subspecies campestris) from China were analyzed to compare the effects of soil pH (6.9 vs. 7.6). The CC grew on the soil of pH6.9 (CC-6.9) contained significantly increased amounts (2.3~4-fold) of pectin, crude protein, vitamin C and vitamin E compared to the control. The contents of ash and minerals (Ca, Fe, Na, Zn, K and Mn) were also significantly increased in CC-6.9. However, CC-6.9 contained 16-75% lower contents of reducing sugars, cellulose and crude fat than CC-7.6. CC-7.6 contained more glucosinolates than CC-6.9; gluconasturtiin (20.68 vs. 0.84 nmol·g-1 wet wt) and gluconapin (202.55 vs. 0.15 nmol·g-1 wet wt). In conclusion, CC-6.9 had an improved texture (high pectin and low cellulose) and nutritional value (high in protein, Ca, Fe, Zn, vitamin C, and E), whereas CC-7.6 had better taste (high in reducing sugars) and anticancer functionality (high in glucosinolates).

조직배양생강과 한국재래종 생강의 유효성분 비교

조만현,함인기,이규희,이종국,이가순,박상규,김태일,이은모 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.4

This study was carried out to compare and analyze the active ingredients of Korean native ginger and rhizome derived from in vitro shoot-tip culture of Korean native ginger. Proximate compositions, mineral nutrients, free sugars, fatty acids, volatile components, 6-gingerol, and 6-shogaol were analysed and evaluated. Korean native ginger was proved to have a little more contents than in vitro rhizome in proximate compositions (crude ash, crude lipid, crude protein, carbohydrate). Mineral nutrient contents (Cu, Fe, Mn, Zn) of in vitro rhizome were higher than those of Korean native ginger. Among the mineral nutrients, the quantity of K was the highest, followed by P, Mg, Na, and Ca. Free sugar contents (fructose, glucose, sucrose) of in vitro rhizome were higher than those of Korean native ginger. Fatty acids containing less than C14 was the major among the fatty acids in ginger. Citral ingredient of the unique aromatic compound of Korean native ginger was stronger than that of the rhizome derived from in vitro shoot-tip culture. Gingerol concentration was increased by shoot-tip culture. 본 연구에서는 생강의 유효성분을 비교분석하기 위하여 한국재래종생강과 조직배양생강의 일반성분, 무기성분, 유리당, 지방산, 휘발성 성분, 그리고 6-gingerol과 6-shogaol 등을 분석하여 평가하였다. 일반성분(조회분, 조지방, 조단백질, 탄수화물)함량은 한국재래종 생강이 조직배양생강보다 다소 높게 나타났다. 무기성분(Cu, Fe, Mn, Zn) 함량은 조직배양생강이 한국재래종 생강보다 높게 나타났다. 무기성분 중에서 가장 많은 것이 K이었으며, P와 Mg, Na, Ca 순으로 많았다. 유리당(fructose, glucose, sucrose)은 조직배양생강이 한국재래종 생강보다 높게 나타났다. 생강은 C14 미만의 지방산이 주요 지방산이었다. Citral 성분은 한국재래종 생강이 조직배양생강 보다 생강의 고유향이 더 강하게 나타났으며, gingerol 함량은 경정배양에 의하여 증가하였다.

토양의 pH (6.9 vs. 7.6)에 의한 배추 (Brassica rapa ssp. campestris) 의 성분에 대한 영향

조만현,함인기,이은모,이조은,강남,임용표,안길환,Jo, Man-Hyun,Ham, In-Ki,Lee, Eun-Mo,Lee, Jo-Eun,Jiang, Nan,Lim, Yong-Pyo,An, Gil-Hwan Institute of Agricultural Science 2010 Korean Journal of Agricultural Science Vol.37 No.1

Functional and nutritional components of 7 cultivars of Chinese cabbage (CC; Brassica rapa subspecies campestris) from China were analyzed to compare the effects of soil pH (6.9 vs. 7.6). The CC grew on the soil of pH6.9 (CC-6.9) contained significantly increased amounts (2.3~4-fold) of pectin, crude protein, vitamin C and vitamin E compared to the control. The contents of ash and minerals (Ca, Fe, Na, Zn, K and Mn) were also significantly increased in CC-6.9. However, CC-6.9 contained 16-75% lower contents of reducing sugars, cellulose and crude fat than CC-7.6. CC-7.6 contained more glucosinolates than CC-6.9; gluconasturtiin (20.68 vs. 0.84 $nmol{\cdot}g^{-1}$ wet wt) and gluconapin (202.55 vs. 0.15 $nmol{\cdot}g^{-1}$ wet wt). In conclusion, CC-6.9 had an improved texture (high pectin and low cellulose) and nutritional value (high in protein, Ca, Fe, Zn, vitamin C, and E), whereas CC-7.6 had better taste (high in reducing sugars) and anticancer functionality (high in glucosinolates).

Plant Regeneration through Leaf Explant Culture of Boxthorn (Lycium chinense Mill.)

조만현,함인기,박상규,이봉춘,이미애,권경학,이은모 한국자원식물학회 2007 한국자원식물학회지 Vol.20 No.3

- This study was conducted to investigate the optimal plant growth regulator level for the shoot formation ofLycium chinenseMill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants werecultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regeneratedthrough in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shootformation had developed from the leaf explants. The shoot formation rate of ‘Jangmyeong’was highest followed by‘Myeongan’, ‘Cheongdae’, and ‘Bullo’. The use of 0.2mg/L BA was critical for enhanced production of shootformation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots weredeveloped and successfully acclimatized to greenhouse.Key words - Leaf, shoot formation, Lycium chinenseMill.Korean J. Plant Res. 20(3):251∼254 (2007)

Efficient Production of Ginger (Zingiber officinale Roscoe) Rhizome by Shoot-Tip Culture

조만현,함인기,이미애,박상규,이은모,권경학 한국자원식물학회 2009 한국자원식물학회지 Vol.22 No.6

High productivity of ginger (Zingiber officinale Roscoe) was obtained from the rhizome produced by shoot-tip culture with Korean native variety, Seosanjong. Seed rhizomes induced by shoot-tip culture were successfully established in the field. The rhizomes induced by both plant or rhizome were higher in emergence rate and faster in days to emergence than those of home seed production. The seed rhizome production induced by shoot-tip culture was two times heavier than that of home seed production. These results suggest that shoot-tip culture might be one of mass propagation methods in seed rhizome of ginger plant.

폐쇄형식물생산시스템을 이용한 감자 경삽묘 육묘시 묘소질과 괴경 생산에 대한 LED 광도의 영향

조만현,함인기,박권서,조지홍,Jo, Man Hyun,Ham, In Ki,Park, Kwon Seo,Cho, Ji Hong 한국작물학회 2020 한국작물학회지 Vol.65 No.4

This study was performed to establish light intensity conditions for producing stem cuttings for aeroponic systems suitable for seed potato production using a closed-type plant production system. Shoot tip cultured plantlets of 'Sumi' and 'Chubaek' potato (Solanum tuberosum L.) were acclimatized, cuttings were collected, and stem cuttings were planted. The seedlings were raised for 40 days at different LED light intensities (60, 120, 180, and 240 μmol·m^-2·s^-1), and were cultivated in an aeroponic system for 80 days. When stem cuttings were raised at 60 μmol·m^-2·s^-1 LED light intensity, the plant height was the longest, at 17.3 cm for 'Sumi' and 16.1 cm for 'Chubaek', and the number of nodes was the highest in both cultivars. The higher light intensities, produced smaller plants with fewer nodes. The leaf areas, SPAD values, and Fv/Fm values differed slightly between cultivars. The fresh weight of stem cuttings, and the production rate of healthy stem cuttings were the highest at 60 μmol·m^-2·s^-1. In the aeroponic system, seedlings raised at 60 μmol·m^-2·s^-1 with LED light intensity showed a difference between the cultivars, but the fresh weight of stems and leaves above the planting plate was the heaviest. In addition, below the planting plate the stem cuttings were longest and the root weight was heaviest at 60 μmol·m^-2·s^-1 LED light intensity. The number of stolons also differed between cultivars, but was greatest for seedlings raised at 60 μmol·m^-2·s^-1 LED light intensity, at 4.2/plant for 'Sumi' and 7.7/plant for 'Chubaek'. At 60 μmol·m^-2·s^-1 LED light intensity, the tuber number and total tuber weight were the best, but the higher the light intensity, the smaller the total tuber number and total tuber weight for both cultivars. In conclusion, when producing potato stem cuttings for aeroponic systems using a closed-type plant production system, the most suitable LED light intensity for raising seedlings was found to be 60 μmol·m^-2·s^-1.

감마선 조사에 의한 국화 화색변이체 선발과 식물체 재생

조만현,함인기,박하승,강시용,최현구,원미경,김태일,박상규,최병준,이은모 한국육종학회 2013 한국육종학회지 Vol.45 No.4

국화 6 품종을 대상으로 감마선(35 Gy) 조사에 의해 유기 된 화색변이체를 선발하고, 식물체 재생을 위한 배지내 식물생 장조절제 적정농도를 구명하여 신품종 육성 가능성을 검토하고 자 수행하였다. 고유품종과 다른 꽃잎의 화색변이율은 ‘SP-410’ 이 15.7%로 가장 높았으며, ‘예스스타’가 10.6%, ‘무지개’가 10.3%순 이었다. ‘무지개’는 이중색(황색/적색, Y7D/R40A) 에서 적색(R42B)으로, ‘예스라인’은 자주색(RP74C)에서 진자 주색(RP63B)으로, ‘예스스타’는 황색/적색(Y4A/RP59A) 이 중색에서 적자주색(RP64A)으로, ‘SP-410’은 백색(W155C)에서 황색(Y2C)으로, ‘예스투게더’의 화심색은 백색(W155C)에서 연보라색(P76B)로, ‘예스우리’의 화심색은 연녹색(YG149A)에 서 짙은 녹색(GY1C)으로 화색이 변한 변이체를 선발하였다. 화색변이체 꽃잎의 신초형성을 위한 배지내 식물생장조정제 의 최적조합은 BA 1.0 mg/L와 NAA 0.5 mg/L, BA 2.0 mg/L 와 NAA 0.5 mg/L의 2조합이였으며, 신초형성율은 품종간에 차이가 있어 ‘예스라인’이 91.6%로 가장 높았다. 감마선 조 사에 의한 화색 변이체의 꽃잎배양 유래 재생식물체는 영양 번식으로 유지되었으며, 3년동안 재배한 결과 화색과 꽃잎의 형태적 특성 차이가 없었다. This study was carried out to investigate the new cultivar production ability of flower color mutants and the optimal plant growth regulator concentration of culture condition for the plant regeneration of 6 Chrysanthemum cultivars using petal culture with flower color mutants induced by 35 Gy of gamma-ray irradiation. The best one was appeared 15.7% of ‘SP-410’, followed by 10.6% of ‘Yes Star’, and 10.3% of ‘Mujigae’ in order at the color mutation rate of petal different from unique cultivar. The flower colors were created such as from bicolor (gold+ red) to red in ‘Mujigae’, purple to red-purple in ‘Yes Line’, bicolor (gold+ red) to red purple in ‘Yes Star’, white to gold in ‘SP-410’. The flower colors on the center of flower were also created such as from white to light purple in ‘Yes Together’, light green to green yellow in ‘Yes Uri’. The two combinations of MS basal medium supplemented with 1.0 mg/L benzylaminopurine (BA) plus 0.5 mg/L naphthalene acetic acid (NAA) and 2.0 mg/L BA plus 0.5 mg/L NAA were proved the best in forming shoot from petal explants of petal culture with flower color mutants. The shoot formation frequency was different by cultivars, and the highest was 91.6% of ‘Yes Line’. The flower color mutants regenerated from petal culture by gamma-ray irradiation were maintained vegetatively and proved no difference on the flower color and the morphological characteristics of petal for 3 years.

배수체 들깨의 주요형질 및 지방산 조성에 관한 연구

김홍수,조만현 東亞大學校生命資源科學大學附設 農業資原硏究所 1996 農業生命資援硏究 Vol.5 No.1

들깨의 형질개량을 위하여 경남재래종 들깨, 적자소, 육성한 4배체 들깨 그리고 2배체 들깨와 적자소간 교잡종에 있어서 주요 형질 및 총지질의 지방산 조성에 관하여 검토했다. 1. 1,000립중은 4배체 들깨가 5.6g으로 최고이었고, 2배체 들깨는 2.1g이었으며, 2배체 들깨와 적자소간의 교잡종은 1.3g이었다. 2. 육성한 4배체 들깨의 유지함량은 50.0%로 최고이었고, 2배체 들깨는 44.7% 이었으며, 2배체 들깨와 적자소간의 교잡종은 27.6%를 나타내었다. 3. Linolenic acid 함량은 육성한 4배체 들깨가 71.9%로 가장 높았고, 2배체 들깨는 65.0%이었다. 4. 육성한 4배체 들깨가 유전자의 누적적 효과에 의하여 1,000립중과 유지함량 그리고 전체 지방산중 불포화지방산인 linolenic acid 등이 2배체 들깨보다 증대효과를 나타내었다고 보아진다. The present study was carried out to investigate the some characters, oil contents and fatty acid composition of diploid perilla of native Kyung-Nam and its breeded tetraploid, red perilla, F?? hybrid obtained by crossing betwen diploid perilla and red perilla. 1. In the 1,000 grain-weight, tetraploid indicated the highest mark with 5.6g, diploid perilla and F?? hybrid obtained by crossing between diploid perilla and red perilla indicated mark with 2.1g and 1.3g, respectively. 2. In the oil contents, breeded tetraploid perilla indicated the highest condition with 50.0%, diploid perilla and F?? hybrid obtained by crossing between diploid perilla and red perilla indicated condition with 44.7% and 27.6%, respectively. 3. In the linolenic acid composition of the total lipids, breeded tetraploid perilla indicated the highest condition with 71.9% and diploid perilla was 65.0%. 4. By the effect of that vreeded tetraploid perilla more increased the 1,000 grain-weight, oil contents and linolenic acid than diploid, it seems that the related genes acted for tetraploid cummulutively.