감마선 조사에 의한 국화 화색변이체 선발과 식물체 재생

국문 초록 (Abstract)

국화 6 품종을 대상으로 감마선(35 Gy) 조사에 의해 유기 된 화색변이체를 선발하고, 식물체 재생을 위한 배지내 식물생 장조절제 적정농도를 구명하여 신품종 육성 가능성을 검토하고 자 수행하였다. 고유품종과 다른 꽃잎의 화색변이율은 ‘SP-410’ 이 15.7%로 가장 높았으며, ‘예스스타’가 10.6%, ‘무지개’가 10.3%순 이었다. ‘무지개’는 이중색(황색/적색, Y7D/R40A) 에서 적색(R42B)으로, ‘예스라인’은 자주색(RP74C)에서 진자 주색(RP63B)으로, ‘예스스타’는 황색/적색(Y4A/RP59A) 이 중색에서 적자주색(RP64A)으로, ‘SP-410’은 백색(W155C)에서 황색(Y2C)으로, ‘예스투게더’의 화심색은 백색(W155C)에서 연보라색(P76B)로, ‘예스우리’의 화심색은 연녹색(YG149A)에 서 짙은 녹색(GY1C)으로 화색이 변한 변이체를 선발하였다. 화색변이체 꽃잎의 신초형성을 위한 배지내 식물생장조정제 의 최적조합은 BA 1.0 mg/L와 NAA 0.5 mg/L, BA 2.0 mg/L 와 NAA 0.5 mg/L의 2조합이였으며, 신초형성율은 품종간에 차이가 있어 ‘예스라인’이 91.6%로 가장 높았다. 감마선 조 사에 의한 화색 변이체의 꽃잎배양 유래 재생식물체는 영양 번식으로 유지되었으며, 3년동안 재배한 결과 화색과 꽃잎의 형태적 특성 차이가 없었다.

다국어 초록 (Multilingual Abstract)

This study was carried out to investigate the new cultivar production ability of flower color mutants and the optimal plant growth regulator concentration of culture condition for the plant regeneration of 6 Chrysanthemum cultivars using petal culture with flower color mutants induced by 35 Gy of gamma-ray irradiation. The best one was appeared 15.7% of ‘SP-410’, followed by 10.6% of ‘Yes Star’, and 10.3% of ‘Mujigae’ in order at the color mutation rate of petal different from unique cultivar. The flower colors were created such as from bicolor (gold+ red) to red in ‘Mujigae’, purple to red-purple in ‘Yes Line’, bicolor (gold+ red) to red purple in ‘Yes Star’, white to gold in ‘SP-410’. The flower colors on the center of flower were also created such as from white to light purple in ‘Yes Together’, light green to green yellow in ‘Yes Uri’. The two combinations of MS basal medium supplemented with 1.0 mg/L benzylaminopurine (BA) plus 0.5 mg/L naphthalene acetic acid (NAA) and 2.0 mg/L BA plus 0.5 mg/L NAA were proved the best in forming shoot from petal explants of petal culture with flower color mutants. The shoot formation frequency was different by cultivars, and the highest was 91.6% of ‘Yes Line’. The flower color mutants regenerated from petal culture by gamma-ray irradiation were maintained vegetatively and proved no difference on the flower color and the morphological characteristics of petal for 3 years.

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