대두 calmoduine유전자 SCaM-5를 발현하는 형질전환 토마토의 병 저항성 검정

이효정,백동원,이옥선,이지영,김동균,정우식,윤재길,이신우,곽상수,남재성,김도훈,윤대진,Lee, Hyo-Jung,Baek, Dong-Won,Lee, Ok-Sun,Lee, Ji-Young,Kim, Dong-Giun,Chung, Woo-Sik,Yun, Jae-Gil,Lee, Sin-Woo,Kwak, Sang-Soo,Nam, Jae-Seung,Kim, Doh-Ho 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.2

농작물 생산에 있어서 병원균 침입에 의한 피해를 줄이는 것은 아주 중요한 과제이다. 식물은 스스로도 생체 방어 신호 전달 기작을 가지고 있지만 그 피해를 줄이는데 있어서 한계가 있다. 본 연구는 콩과 식물에서 분리한 식물생체방어 신호전달 유전자인 SCaM-5를 토마토에 형질전환하여 형질전환 식물체를 작성하고 병 저항성에 관한 실험을 수행한 것이다. SCaM-5 유전자가 형질전환 된 토마토에서는 pathogen-related(PR-5) 유전자를 항상 발현시킴으로서 계속적으로 식물 생체방어 신호기작을 활성 시킨다는 사실을 확인하였다. 또한, SCaM-5를 형질전환 시킨 토마토는 식물에 막대한 피해를 주는 중요한 곰팡이 (P. capsici와 F. oxysporm)와 bacteria (Pst DC3000)에 대하여 병 저항성을 가진다는 것을 검증하였다. The calmodulin as a Ca$^{2+}$-binding protein mediates cellular Ca$^{2+}$ signals in response to a wide array of stimuli in higher eukaryotes. Plants produce numerous calmodulin isoforms that exhibit differential gene expression patterns and sense different Ca$^{2+}$ signals. SCaM-5 is a soybean calmodulin that is involved in plant defense signaling. Here, we constructed a SCaM-5 CDNA under control of CaMV 35S promoter and transformed it into tomato (Lycopersicon esculentum). The constitutive over-expression of SCaM-5 in tomato plants exhibited a high levels of pathogenesis-related (PR) gene expression, and conferred an enhanced resistance to two fungal pathogen (Phytophthora capsici, Fusarium oxysporum), and a bacterial pathogen, Pseudomonas syringae pv. tomato DC3000. Thus, this results collectively suggest that SCaM-5 plays an important role in plant defense of tomato.

신생아에서 수신증의 심한 정도와 방광요관역류 존재와의 연관성

이효정,노성훈,이수연,김민선,이대열,Lee, Hyo-Jung,Noh, Seong-Hoon,Lee, Soo-Yeon,Kim, Min-Sun,Lee, Dae-Yeol 대한소아신장학회 2009 Childhood kidney diseases Vol.13 No.1

Purpose : The increasing use of ultrasonography has allowed for an increase in the of the detection of congenital hydronephrosis, and the clinical outcomes of congenital hydronephrosis are widely varied. In this study, the necessity of voiding cystourethrography in neonate with hydronephrosis to rule out vesicoureteral reflux (VUR) was evaluated. Methods : Between January 2004 and December 2007, we reviewed the medical record of 157 childrens with congenital hydronephrosis detected within 1 month of age. The severity of hydronephrosis was graded by SFU (Society of Fetal Urology) system, and anterior posterior pelvic diameter (APPD). We evaluated the relationship between severity of hydronephrosis and incidence of VUR by using SPSS windows version 16.0. A P-value<0.05 is considered to be statistically significant. Results : Total renal unit number was 254, and 20(7.8%) renal units had VUR. We did not find any relationship between hydronephrosis grade the presence of VUR grade (P>0.05). In addition, there was no statistical significance between APPD, laterality of hydronephrosis and VUR incidence. However, renal units with VUR had lower spontaneous resolution rate (P<0.05), compared to renal units without VUR. Conclusion : In this study, there was no statistical significance between the severity of hydronephrosis and presence of VUR. Therefore, voiding cystourethrogram is recommended for all children with hydronephrosis to rule out VUR, regardless of the severity of hydronephrosis. 목 적 : 산전 초음파 이용의 증가로 인하여 선천성 수신증 발견 빈도가 증가하고 있고 VUR이 수신증의 원인 중 하나이다. 본 연구에서는 선천성 수신증의 정도와 VUR의 연관성을 조사하여 배뇨성 방광요도 조영술의 필요성을 알아보고자 하였다. 방 법 : 2004년 1월 1일 부터 2007년 12월 31일 까지 생후 1개월 이내에 선천성 수신증이 진단된 157명의 환아 254구의 신장을 대상으로 수신증의 등급 및 신우 전후직경이 VUR의 빈도와 연관성이 있는지를 연구하였다. 통계 분석은 SPSS 16.0을 이용하여 Chi-Square 법, ANOVA 법으로 분석하였으며, P값이 0.05 미만인 경우를 통계적으로 유의한 것으로 분석하였다. 결 과 : 수신증이 있는 254구의 신장 중 7.8%에 해당하는 20구의 신장에서 VUR이 동반되었다. 수신증 등급과 신우 전후직경은 VUR의 빈도와는 의미있는 상관관계가 없었고, 좌측이 우측보다 수신증의 정도와 VUR 등급이 유의하게 높았으며 VUR이 존재하는 경우 수신증의 자연 소실률이 현저히 낮은 것으로 나타났다. 결 론 : 본 연구에서 수신증 등급과 VUR의 유무와는 통계적 유의성이 없었다. 따라서 수신증의 심한 정도에 관계없이 모든 환아에서 VUR 유무를 확인하기 위해 배뇨성 방광요도조영술은 시행되어야 한다.

약동학적 파라미터를 이용한 시간경로 마이크로어레이 자료의 군집분석

이효정,김별아,박미라,Lee, Hyo-Jung,Kim, Peol-A,Park, Mi-Ra 한국통계학회 2011 응용통계연구 Vol.24 No.4

시간경로 마이크로어레이 자료 분석의 주요 목적 중의 하나는 유전자들의 시간에 따른 발현수준의 변화를 고려함으로써 발현패턴에 기초한 유전자들의 그룹을 찾기 위한 것으로, 군집분석을 위한 다양한 알고리즘들이 제안되었다. 본 연구에서 시간경로 마이크로어레이 자료에 대한 군집분석을 위해 두 약물제제 간 생물학적 동등성을 평가하기 위한 약동학 시험에서 사용되는 약동학적 파라미터 값에 기초한 군집분석을 제안하였으며 이를 실제 데이터 및 모의실험 자료에 적용하여 유용성을 검토하였다. A major goal of time-course microarray data analysis is the detection of groups of genes that manifest similar expression patterns over time. The corresponding numerous cluster algorithms for clustering time-course microarray data have been developed. In this study, we proposed a clustering method based on the primary pharmacokinetic parameters in the pharmacokinetics study for assessment of pharmaceutical equivalents between two drug products. A real data and a simulation data was used to demonstrate the usefulness of the proposed method.

교량 주변 하도구간에서 하상변동 및 유사농도 분포 특성분석

이효정,정도준,안승섭,Lee. Hyo-Jung,Jung. Do-June,Ahn. Seung-Seop 한국방재학회 2008 한국방재학회 학술발표대회논문집 Vol.2008 No.1

Recently many studys have been continued Nak-Dong river. This study recognized the importance about a rivers floor change. The Nak-Dong main river of railroad bridge(2.423Km) waegwan-eup, Cilgok-gun, Gyeongsangbuk-do analyzed to the SED2D-WES model. This study recognized the difference of the model according to the existence and nonexistence of the bridge piers. At a result of mean velocity current is higher in bridge option appeared in this case. As well sediment transportation model show that river bed change appear the part of velocity is low.

집단 및 가족기반연구에서의 유전적 연관성 분석 고찰: 방법론과 소프트웨어

이효정,김민지,박미라,Lee, Hyo-Jung,Kim, Min-Ji,Park, Mi-Ra 한국통계학회 2010 응용통계연구 Vol.23 No.1

최근 단일염기다형성 및 일배체형을 이용한 질병-유전자간 연관성연구가 많이 진행되고 있으며, 이를 위한 다양한 분석방법과 분석도구가 개발되고 있다. 그러나 통합 소프트웨어는 충분히 확립되지 못하였으며, 각 소프트웨어가 제공하는 분석방법 및 양식에 차이가 많아 연구자가 적절한 것을 선택하기가 쉽지 않다. 본고에서는 유전적 연관성연구를 사전분석단계, 집단기반연구방법, 가족기반연구방법으로 나누어 각각의 목적에 따른 분석방법을 고찰하고, 이의 분석을 위한 주요 소프트웨어로서 FBAT, SAS/Genetics, SAGE, R의 지원내용과 방법을 비교하였다. Recently, there have been lots of study for disease-genetic association using SNPs and haplotypes. Statistical methods and tools for various types of data are developed by many researchers. However, there is no unified software which can handle most of major analysis, and the methods and manners to deal with data are quite different through softwares. And thus it is not easy to researcher to choose proper software. In this study, we devide analyzing procedures into three steps: preliminary analysis, population-based analysis and family-based analysis. We review the statistical methods for each step and compare the features of the FBAT, SAS/Genetics, SAGE and R as major integrating softwares for genetic study.

보기익혈 복합방, 바이오 큐의 면역조절 효과

이효정,길재호,백남인,라정찬,김성훈,Lee, Hyo-Jung,Khil, Jae-Ho,Baek, Nam-In,Ra, Jeong-Chan,Kim, Sung-Hoon 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

Bio-Q is a modified prescription with the activities of supplementing Qi and blood in human body. In the present study, immunomodulatory effect of Bio-Q was examined. After oral administration of Bio-Q for 7 days to Balb/c mice, splenocytes were isolated and immunological experiments were performed. Bio-Q significantly increased the proliferation of splenocytes exposed to concanavalin A (Con A), while it did not in case of lipopolysaccharide (LPS) stimulation. Bio-Q also significantly increased CD3/CD19, CD4/CDB and NK cells by flow cytometric analysis. In addition, Bio-Q significantly enhanced the level of $INF-\gamma$ in splenocytes, but not $TNF-\alpha$ by ELISA. These results strongly suggest that Bio-Q has immunomodulatory activity through the regulation of T cell mediated immune pathway.

포스트휴머니즘 표현특성을 적용한 미디어테크 실내공간에 관한 연구

이효정(Lee, Hyo-Jung ),윤은경(Yun, Yean-Kyung) 한국실내디자인학회 2016 한국실내디자인학회 학술대회논문집 Vol.2016 No.10

Although we can acquire much information from anywhere in this information age, there is difficulty in obtaining practical and useful information and re-creating it. This indicates that the library of the future, unlike that of the past, must take into account not only access to information but also utilization. Meeting this requirement, libraries are seeing changes in concepts, more complex programs, and diversification of information media, which all help to transform libraries into a new place of culture. While such trend is surfacing in the form of mediatheque, Korea is still at a transitional period in which such library spaces are called media libraries and digital libraries. Under such circumstances, the current study reviews the background in which mediatheque surfaced, as well as spatial programs, in an attempt to understand the new trend. Furthermore, the current study will focus on the expression attributes of post-humanism, a school of thought triggered by the reconstruction of the human concept after the rapid development of science and technology. As a result of various studies of cases in Korea and abroad, the study finds that Korea is at a stage in which it is beginning to accept cultural and artistic programs in the libraries, and while communication using cutting-edge media technologies is vibrant in Korea, other expressive attributes are more passively used compared to cases in other countries. In conclusion, such issues must be understood better, and there must be increased attempts to understand the necessity for mediatheque in preparation for a new era; as there is an increasing requirement for a more variegated spatial formation and complex spaces for various programs, more research must be conducted in this regard.

모바일 앱 기반 소셜 데이팅 콘텐츠의 분석과 설계

이효정(Hyo-Jung Lee),최윤호(Yoon-Ho Choi),이병엽(Byoung Yup Lee),이재원(Jae-Won Lee) 한국콘텐츠학회 2014 한국콘텐츠학회논문지 Vol.14 No.4

온라인데이팅에서 변화된 소셜데이팅 서비스에 대해서 개념,현황과 시장규모,발전과정을 살펴보고자 하였다. 스마트 디바이스의 보급율이 높아지면서 데이팅서비스의 영역은 오프라인에서 온라인으로 확대되어 가고 있으며, 온라인데이팅은 소셜데이팅 혹은 소개팅 어플리케이션이라는 새로운 콘텐츠 시장으로 발전하였다. 이에 따라, 본 연구는 소셜데이팅 어플리케이션 시장에 대한 현황과 개념을 정리하고 관련한 문헌과 연구를 고찰하였으며, 소비자들에게 주로 사용되고 있는 소셜데이팅 어플리케이션을 특징별로 분류하였다. 성공적인 사례에 대한 비교 분석과 비지니스 모델 분석 등을 수행함으로써 소셜데이팅 어플리케이션이 가진 문제점과 개선해야 하는 기능들은 정리하였으며, 분석된 경쟁전략을 적용하여 모바일 어플리케이션에 기반을 둔 신규의 소셜데이팅 어플리케이션 시스템을 설계하고 서비스발전의 방향성을 제시하였다. Social dating contents that transformed from online dating service needs understanding about the market status, magnitude, business concept and related area, and its development process. Now a days the field of dating service had expanded from off-line to on-line, and then started to be changed to social dating services with the development of the highest smart phone penetration rate. Therefore, we investigated about the market, business concept, and its functions of social dating application, and then classified the typology of those social dating applications according to feature with utilization rate of user. We had described about its partial deficits and improvements on the social dating application through comparative analysis and business model analysis about successful case. As conclusions, we suggested future strategies of competition and directivity with an improving way about concrete plan for industry development of social dating application.

IPA를 이용한 계절별 국립수목원 이용객의 서비스 질 만족도 연구

이효정 ( Hyo Jung Lee ),김재현 ( Jae Hyun Kim ),임윤정 ( Yun Jung Lim ),장주연 ( Chu Youn Chang ),태유리 ( Yoo Lee Tae ),홍성권 ( Sung Kwon Hong ) 한국산림과학회 2011 한국산림과학회지 Vol.100 No.4

The purpose of this study was to investigate the satisfaction of service quality for seasonal visitors of Korea National Arboretum using Importance-performance analysis. Data were obtained from visitors of Korea National Arboretum on spring, summer, autumn. Results of analysis showed that all season visitor`s satisfaction was high. The IPA was undertaken with total 24 variables about service quality of arboretum. Results of IPA was founded that summer visitors want to improve some services (information facilities, forest museum, conveniences, etc). Autumn visitors, on the other hand, thought that some services (kindness, active responsiveness, variety of plant, etc) are overinvested. As a results, there is differences between importance and satisfaction about seasonal service quality.

치주 질환과 동맥경화성 심혈관 질환의 관계성에 대한 고찰과 치주처치

이효정(Hyo-Jung Lee) 대한치과의사협회 2013 대한치과의사협회지 Vol.51 No.9

This paper reviews a current view regarding the association between periodontitis and atherosclerotic cardiovascular diseases (ACVD). Many evidences have suggested that there exist biological mechanisms by which periodontitis can lead to ACVD. Periodontal infection can lead to direct bacterial invasion into endothelial tissues through the blood stream, then the bacteria can activate the host inflammatory response followed by atheroma formation, maturation and exacerbation. Also, chronic periodontal infections may indirectly induce endothelial activation or dysfunction through a state of systemic inflammation as evidenced by elevated plasma acute proteins, IL-6 and fibrinogen as well. There is moderate evidence that periodontal treatment can reduce systemic inflammation and improvement of both clinical surrogate markers. But there is no periodontal intervention study available on primary ACVD prevention. There is consistent and strong epidemiologic evidence, including in vitro, animal and clinical studies, that periodontitis imparts increased risk for future ACVD. However, evidences from intervention trials to date are not sufficient to confirm the multi directional causality of periodontitis in ACVD etiology. Well-designed intervention trials on the impact of periodontal treatment on the prevention of ACVD outcomes are needed.