김치유산균용 발현벡터 pSJE6c 개발과 이를 이용한 외래 유전자 발현

이강욱,박지영,이지연,이황아,백창운,조현덕,김주연,권건희,천지연,김정환,Lee, Kang-Wook,Park, Ji-Yeong,Lee, Ji-Yeon,Lee, Hwang-A,Baek, Chang-Un,Jo, Hyeon-Deok,Kim, Joo-Yeon,Kwon, Gun-Hee,Chun, Ji_Yeon,Kim, Jeong-Hwan 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.25 No.4

본 연구실에서 개발한, 김치에서 분리한 Leu. mesenteroides SY2 유래 pFMBL1 을 바탕으로 구축한 셔틀벡터인, pSJE[7]를 외래유전자 발현에 적합하게 개량한 발현벡터를 구축하였다. Lactococcus lactis LM0230에서 분리한 프로모터 P6C를 pSJE에 도입하였다. P6C 염기서열을 지닌 oligonucleotide 쌍을 따로 제조한 후 annealing을 통해 짧은 DNA 단편을 얻어서 제한효소 처리후 pSJE에 도입하여 pSJE6c를 구축하였다. PSJE6c 효능 검증을 위해서 외래 유전자인 aga와 lacZ를 각각 pSJE6c에 도입하였다. P6C 프로모터와 비교를 위해 고유 프로모터를 지닌 유전자들도 각각 pSJE에 도입하였다. 재조합 plasmid들을 electroporation 방법으로 Lactobacillus brevis 2.14 균주에 도입하고 재조합균주들의 생육곡선과 효소역가 그리고 slot blot으로 전사체 농도를 측정하였다. 결과를 보면 PSJE6c에 클로닝 된 유전자들이 pSJE상의 유전자보다 효소역가들이 약 1.5배에서 2배 정도로 높았다. 전사체 농도 측정 결과도 pSJE6c 들에서 더 많은 전사체가 생성됨을 보여주었다. 이상 결과들은 효율적인 발현벡터들의 사용을 통해서 김치유산균에서 외래유전자 발현 효율을 높일 수 있음을 보여준다. Development of expression vectors is important for the basic and applied researches on kimchi LAB (lactic acid bacteria). An expression vector, pSJE6c was constructed by inserting P6C promoter sequence from Lactococcus lactis into pSJE, a shuttle vector for E. coli and Leuconostoc species. To test the efficiency of pSJE6c, aga ($\alpha$-galactosidase) and lacZ ($\beta$-galactosidase) genes were expressed in Lactobacillus brevis 2.14. Compared to the pSJE, expression levels of both genes were increased, indicating P6C promoter was better than indigenous promoters. Enzyme activities of L. brevis cells harboring pSJE6caga (pSJE6c with aga) or pSJE6Z (pSJE6c with lacZ) were 1.5-2 fold higher than those with pSJEaga (pSJE with aga) or pSJEZ (pSJE with lacZ). More RNA transcripts were detected in cells harboring pSJE6c based recombinant plasmid. The results indicated that heterologous gene expressions in kimchi LAB could be improved significantly by use of efficient expression vectors.

유방암 치료에 대한 한의 임상 가이드라인 개발을 위한 기초연구

이강욱,유화승,이진선,이남헌,Lee, Kang-Wook,Yoo, Hwa-Seung,Lee, Jin-Sun,Lee, Nam-Hun 대한한방부인과학회 2016 大韓韓方婦人科學會誌 Vol.29 No.2

Objectives : The purpose of this study was to present the clinical practice guidelines for breast cancer through the analysis of existing clinical practice guidelines in Western and Chinese medicine. Methods : The literature search was performed regarding conventional and integrative medical treatment of breast cancer using Google Scholar, KIOM OASIS, PUBMED and Jisan library of Daejeon university. Results : Breast cancer is the fifth most common cancer in Korea. Recent studies revealed that combining conventional and integrative medical treatment can reduce the sequela of surgical operation and improve survival rate. However there isn’t still a systemic clinical guideline for breast cancer in korean medicine. This study will be a preliminary study to establish clinical practice guidelines of Korean Medicine for breast cancer.Conclusions : Further objective and systemic clinical studies related to Korean Medicine are needed to develope more advanced clinical practice guidelines of breast cancer.

한인진(韓茵蔯), 울금(鬱金), 산사(山査), 단삼(丹蔘) 발효복합추출물이 RAW264.7 및 HUVEC 세포에서 나타나는 이상지질혈증 관련 염증인자 발현 및 항산화에 미치는 영향

이강욱,조현경,유호룡,설인찬,김윤식,Lee, Kang-wook,Cho, Hyun-kyoung,Yoo, Ho-ryong,Seol, In-chan,Kim, Yoon-sik 대한한방내과학회 2018 大韓韓方內科學會誌 Vol.39 No.4

Objectives: To investigate the effect of fermented extract of Artemisiae Iwayomogii Herba, Curcumae Longae, Crataegi Fructus and Salviae Miltiorrhizae Radix (FMH) on anti-inflammation associated with dyslipidemia and anti-oxidation in RAW264.7 and HUVEC cells. Methods: The total polyphenols, total flavonoids, DPPH radical scavenging activity, ABTS radical scavenging activity, and cytotoxicity of FMH were measured. RAW264.7 cells treated with FMH were tested for production of NO, and for cytokine and LTB4 levels and HUVEC cells treated with FMH were examined for production of cDNA of genes related to inflammation. Results: 1. FMH contained polyphenols and flavonoids. The DPPH and ABTS radical scavenging activity of FMH increased in a concentration-dependent manner. 2. FMH treatment inhibited the production of nitric oxide (NO), cytokines, and LTB4 in RAW264.7 cell when compared to the untreated control group. 3. FMH decreased the transcription of pro-inflammatory genes, whereas it increased transcription of anti-inflammatory genes, in HUVEC cells. Conclusion: FMH is effective as an antioxidant and for treatment and prevention of dyslipidemia, atherosclerosis, ischemic heart disease, stroke, and other cardiocerebrovascular diseases.

완전삽입형 인공망막 구현을 위한 인공망막모듈 개발

이강욱,카이호 요시유키,후쿠시마 타카후미,타나까 테츠,고야나기 미쯔마사,Lee, Kang-Wook,Kaiho, Yoshiyuki,Fukushima, Takafumi,Tanaka, Tetsu,Koyanagi, Mitsumasa 대한의용생체공학회 2010 의공학회지 Vol.31 No.4

To restore visual sensation of blind patients, we have proposed a fully implantable retinal prosthesis comprising an three dimensionally (3D) stacked retinal chip for transforming optical signal to electrical signal, a flexible cable with stimulus electrode array for stimulating retina cells, and coupling coils for power transmission. The 3D stacked retinal chip is consisted of several LSI chips such as photodetector, signal processing circuit, and stimulus current generator. They are vertically stacked and electrically connected using 3D integration technology. Our retinal prosthesis has a small size and lightweight with high resolution, therefore it could increase the patients` quality of life (QOL). For realizing the fully implantable retinal prosthesis, we developed a retinal prosthesis module comprising a retinal prosthesis chip and a flexible cable with stimulus electrode array for generating optimal stimulus current. In this study, we used a 2D retinal chip as a prototype retinal prosthesis chip. We fabricated the polymide-based flexible cable of $20{\mu}m$ thickness where 16 channels Pt stimulus electrode array was formed in the cable. Pt electrode has an impedance of $9.9k{\Omega}$ at 400Hz frequency. The retinal prosthesis chip was mounted on the flexible cable by an epoxy and electrically connected by Au wire. The retinal prosthesis chip was cappted by a silicone to pretect from corrosive environments in an eyeball. Then, the fabricated retinal prosthesis module was implanted into an eyeball of a rabbit. We successfully recorded electrically evoked potential (EEP) elicited from the rabbit brain by the current stimulation supplied from the implanted retinal prosthesis module. EEP amplitude was increased linearly with illumination intensity and irradiation time of incident light. The retinal prosthesis chip was well functioned after implanting into the eyeball of the rabbit.

곤륜(崑崙).소부(少府) 침자(鍼刺)가 하장간막신경(下腸間膜神經) 활성(活性)과 혈압(血壓) 및 심박수(心搏數)에 미치는 영향(影響)

이강욱,윤여충,김정상,나창수,Lee Kang-Uk,Yun Yeo-Chung,Kim Jeong-Sang,Na Chang-Su 경락경혈학회 2002 Korean Journal of Acupuncture Vol.19 No.2

Objectives : The aim of the present study is to observe effects of acupuncture related with the nerve system, Inferior mesenteric nerve activity(IMNA) was examined on BL60 acupuncture closely connected with the bladder and HT8 with little tied. Materials and methods : Acupunctures were performed during 100 seconds after stabilization. IMNA was measured by micromanipulator, preamplifier, mean arterial pressure(MAP) and heart rate were observed by blood pressure transducer, 4-channel transducer amplifier in anesthetized rat. Results : On BL60 acupuncture, the heart rate was not significantly decreased but IMNA, MAP were significantly decreased and Maximum peak of IMNA was increased during inserted acupuncture, decreased after ejected acupuncture respectively. On HT8 acupuncture, IMNA was decreased during acupuncture than before acupuncture but the others did not showed signigicant difference. Conclusions : Our results demonstrated a meridian interaction between BL60 acupoint in the bladder channel and Inferior mesenteric sympathetic nerve related to the bladder. This fact suggest that BL60 acupuncture have effect on the bladder through the nerve system. Meanwhile, IMNA was decreased during HT8 acupuncture. This result is supposed HT8 to have not meridian function but acupoint function by another mechanism. It needs to be closely examined other meridians and nerve by the optimal approaching method.

노인성 치매

이강욱,Lee, Gang-Uk 한국건강관리협회 1997 건강소식 Vol.21 No.9

복합단백발효사료와 어분의 산란계에 대한 사양가치 비교시험

이강욱,김한경,주현규,박주식 ( K . W . Lee,H . K . Kim,H . K . Ju,J . S . Park ) 한국축산학회 1973 한국축산학회지 Vol.15 No.4

To compare the nutritive value of fish meal with that of fermented compound protein meal, the feeding trial with 288 laying hens of shaver strain was carried out for 8 weeks. The results obtained are as follows; 1. Experimental fermented compound protein meal contained less protein (39.5%) than imported fish meal (67%) and domestic fish meal (56%). However, moisture content of domestic fish meal (19.8%) was higher than that of fermented compound protein meal (10.6%) and imported fish meal (9.8%). It was found that the content of Ca, P and methionine in forign fish meal was superior to those in the fermented or domestic fish meal. 2. In growth rate, there was no statistical difference between the treatments. However, the amount of feed consumed was higher for the hens fed fermented compound protein meal than for those fed fish meal, although no significant difference was observed between the source of fish meal. 3. There was no statistical significance among the treatment in egg production and egg weight, but the hens feed fermented compound protein meal produced a little more eggs of heavier egg weight than those fed control diet. 4. These results indicate that fermented compound protein meal up to 10% level can be used in the laying hens diet satisfactorily.

건설산업 관점의 국가 리스크 평가방식 소개

이강욱,Lee, Gang-Uk 한국건설관리학회 2017 건설관리 : 한국건설관리학회 학회지 Vol.18 No.1

국내 토목산업의 설계-시공 균형발전 방안 도출에 관한 연구

이강욱,한승헌,박형근,진경호,Lee, Kang Wook,Han, Seung Heon,Park, Hyung Keun,Chin, Kyung Ho 대한토목학회 2013 대한토목학회논문집 Vol.33 No.1

최근 해외건설시장에서는 산업설비 분야를 중심으로 기획, 설계, 구매조달, 시공을 통합하는 EPC(Engineering, Procurement, & Construction) 형태의 발주물량이 증가하고 있으며, 토목산업 분야에서도 점차 Design-Build 발주사례가 증가하고 있다. 이러한 발주환경의 변화에 따라 프로젝트종합관리, 기본설계 등 건설엔지니어링의 중요성이 대두되고 있으나, 아직까지 국내 건설산업은 시공 위주의 산업구조에서 벗어나지 못하고 있다. 따라서 본 연구는 국내 토목산업의 엔지니어링 역량을 제고하고, 장기적으로는 설계-시공 균형발전을 위한 산업 차원의 발전방안을 제시하는데 목적을 두고 진행되었다. 이를 위해 우선 국내외 건설산업 현황을 분석하였으며, 국내 건설업체들의 인식수준 및 니즈 파악을 위한 설문조사와 발전방안의 실효성 검토를 위한 전문가 심층면담을 순차적으로 진행하였다. 본 연구에서 도출한 국내 토목산업의 설계-시공 균형발전 방안은 1) 토목설계업 관리체계 효율화, 2) 토목설계업 관련 제도 선진화, 3) 고부가가치 우수인력 육성, 4) 엔지니어링조합 및 컨소시엄 구성, 5) 해외건설 협력기구 조직 및 운영으로 구분하여 제시되었다. In the recent global construction environment, EPC contracts that integrate planning, design, procurement, and construction have been widely used for industrial facilities. Similarly, as the importance of the engineering capability such as project management consulting(PMC) and front end engineering design(FEED) has been increased, design-build contracts also have been gradually adopted for infrastructure projects. Whereas, the Korean infrastructure industry has fragmentary features which mainly emphasize on the construction sector. Therefore, this study aims at developing strategies for enhancing the engineering capability and for balancing the competitiveness between engineering and construction sectors in the Korean infrastructure industry. To this end, the authors first carried out market analysis for both global and domestic market. Then, a structured survey and expert in-depth interviews were sequentially performed in order to reflect practitioners' perspectives and needs for institutional strategies. As a result, five strategies drawn from this study are as follows: 1) rearranging the government's roles for supporting the engineering sector; 2) establishing institutional bases being compatible with global standards; 3) fostering value-added workforce for undertaking complex tasks; 4) constructing an consortium between an engineering association and general contractors; and 5) building a nationwide collaborative networks for overseas market expansion.

십사경맥(十四經脈) 중 족궐음(足厥陰) 경락(經絡)의 병증(病症)에 대한 고찰(考察)

이강욱,윤대환,박찬규,정종길,나창수,Lee Kwang-Uk,Youn Dae-Hwan,Park Chan-Gyu,Jeong Jong-Gil,Na Chang-Su 경락경혈학회 2002 대한침구의학회지 Vol.19 No.1

In the oriental medicine, the biological mechanism and pathogenic change have been based on the meridian. A Liver channel of those belong to the wood in the five element system, the Leg channel of the twelve primary channels, Absolute Yin(厥陰) in the three Yin channels and connect with Liver organ. Through the study on a liver channel, the results were as follows. Leg Absolute Yin is indicated for disease affecting the lower abdomen, uro-genital system, knee and thigh. Symptons associated with mind regulation system : hypochondria, a feeling of restlessness and the deficiency of Liver Yin. Symptons associated with disease of Absolute Yin.