6-(3,4-디클로로페닐)아미노-7-클로로-5,8-퀴놀린디온의 항진균작용 및 안전성 평가

윤여표,김동현,이병무,허문영,정해문,강혜영,최정아,김도희,유충규 성균관대학교 약학연구소 1998 成均藥硏論文集 Vol.10 No.1

6-(3.4-Dichlorophenyl)amino-7-chloro-5.8-quinolinedione (RCK50) was tested for antifungal activities in mice systemically infected with Candida albicans. The therapeutic potential of RCK50 was also assessed in comparison with ketoconazole. RCK50 had ED_50 0.22±0.01 ㎎/㎏. Ketoconazole as a positive control had ED_50 6.00±1.70 ㎎/㎏. Intraperitoneally administered RCK50 at the ED_50 for 7 days and 14 days reduced Candida albicans colony count in the kidneys and liver. And administered RCK50 at the ED_50 for 14 days improved survival rates. The genotoxicities of RCK50 had been evaluated. RCK50 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. RCK50 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. These results indicate that RCK50 has no genotoxic potential under these experimental conditions. Acute oral toxicity studies of RCK50 were carried out in ICR mice of both sexes. RCK50 did not show acute oral toxicities and LD_50 values were over 2.850 ㎎/㎏ in ICR mice.

腰痛에 對한 東醫學的 病理 및 臨床治療(I)

尹汝忠,鄭遇悅,羅昌洙,鄭鉉雨 대한동의병리학회 1996 동의생리병리학회지 Vol.10 No.2

현재 인류의 60% - 80%가 腰痛을 앓고 있는 실정이다. 이러한 腰痛을 치료하기 위하여 《黃帝內徑》 이래 많은 醫家들과 현재의 臨床家들이 다방면의 治療療法 개발 및 臨床例들을 보고하고 있으나, 아직까지도 대다수의 국민들이 腰痛을 호소하고 있다. 이에 본 연구자들은 이러한 腰痛에 대한 病因, 病機 및 治法들을 연구하여 臨床家들에게 조금이나마 도움이 되었으면 하여 보고하게 되었다. 東西醫學的으로 腰痛의 개념을 정립하고, 《東醫寶鑑》에 準하여 腰痛의 種類, 腰痛의 原因 및 病理機轉, 또한 病程에 따른 東西醫學的인 分類, 治療方法, 治療藥物, 그리고 攝生과 豫防法에 대하여 정리하였다. 腰痛의 原因으로는 寒濕과 房營傷이 가장 많았고, 그 다음으로는 風熱, 營養缺乏, 慢性疾患등이 있었으며, 病理機轉으로는 嗜慾勞傷, 挫閃跌墜등이 있었다. 그리고 十種腰痛과 卒腰痛, 久腰痛으로 분류하여 溫補腎陽, 行氣活血 등의 治法들을 사용하였다. 西醫學的으로는 急性 腰部捻挫, 慢性腰部捻挫, 椎間板脫出症등으로 大別되며, 이러한 腰痛을 예방하기 위하여서는 좋은 자세를 유지하는 것이 가장 중요하다. Lumbago is painess of lumbar region or lower limb, and this disease is classified backache, lumbosacral pain, lumbocrural pain. Lumbage is very common, with up to 60% to 80% of population, this disease is one of a lots painess giving on population. The resul of study on lumbago's agent, pathogenesis, symptoms of disease, principle of treatment, and therapy were as follows : 1. Agents of lombago were cool-dampness pathogenic factor, sexual strain, and wind-heat pathogenic factor, hypoalimemtation, chronic disease etc. 2. Pathogenesises of lombago were six climatic, seven emotions, internal injury caused by overstrain, sprain etc. 3. In Oriental medicine, symptoms of lumbago were lumbago due to the kidney deficiency, lumbago due to retention of phlegm, lumbago due to retention of food, lumbago due to blood stasis, lumbago due to pathogen, lumbago due to cool pathogen etc. 4. In Western medicine, symptoms of lumbago were acute lumba muscle strain, chronic lumba muscle strain, disc etc. 5. Principles of treatment of lumbago were promoting flow of qi(氣) and blood circulation, warming and recuperating the kidney-yang(陽) etc. 6. Therapies of lumbago were acupuncture, medicayion, electrotherapy, chiroplatic, coll and heat therapy etc. 7. Herbs were radix achyranthis, caulis cistanchis, cornu cervi parvum, cornu cervi, cortex eucommiae, semen cuscutae, semen psoraleae etc. 8. In order to prevent lumbago, the best priciple is maintain good attitude.

PS-MMA 공중합체를 이용한 PS-PMMA 쌍의 상호작용 파라미터 설정

정순량,윤여근 又石大學校 1987 論文集 Vol.9 No.-

The could points(phase separation temperature) of the blends of polystyrene with PS-NMA random copolymer with various molecular weight and components, were determined by optical observation. The interaction parameter Λ of PS-PMMA pair was indirectly derived from these cloud points. The Λ value investigated was ca. 1.37㎈/㎤, which was in good agreement with the experimental results.

Ba-Ferrite를 이용한 자기 연마재 개발

김희남,송승기,정윤중,윤여권,김희원,조상원,심재환 한국안전학회 2003 한국안전학회지 Vol.18 No.2

The magnetic polishing is the useful method to finish some machinery fabrications by using magnetic power. This method is one of the precision techniques and has an aim for clean technology in the transportation of the pure gas in the clean pipes. The magnetic abrasive polishing method is not so common in the field of machine that it is not known to widely. There are rarely researcher in this field because of non-effectiveness of magnetic abrasive. Therefore, in this paper we deals with the development of the magnetic abrasive with the use of Ba-Ferrite. In this development, abrasive grain WA has been made by using the resin bond fabricated at low temperature. And magnetic abrasive powder was fabricated from the Ba-Ferrite which was crushed into 200 mesh. The XRD analysis result shows that only WA abrasive and Ba-Ferrite crystal peaks were detected, explaining that resin bond was not any more to contribute chemical reaction. From SEM analysis, we found that WA abrasive and Ba-Ferrite were strongly bonding with each other.

디젤분진이 사람 동맥 평활근 세포(VSMC)에 미치는 영향

임용,김수연,정규혁,정진호,문창규,윤여표 한국환경독성학회 2004 환경독성보건학회지 Vol.19 No.1

The purpose of the present study was to examine the effect of diesel exhaust particles on human aortic vascular smooth muscle cells(VSMCs). DNA synthesis, cell viability and morphology of VSMCs after treatment of diesel exhaust particles(DEP) and fine particulate matter(PM_(2.5)) were assayed. PM_(2.5) inhibited the DNA synthesis of VSMCs in a concentration-dependent manner, whereas DEP did not affect VSMCs up to 50㎍/mL . These results were confirmed by morphological examination of VSMCs. PM_(2.5) showed a dose-dependent cytotoxicity of VSMCs by MTT assay. Fraction 4(organic acids) and fraction8(moderately polar compounds) showed the most potent inhibition of DNA synthesis of VSMCs, and fraction7(slightly polar compounds), fraction9(higher polar compounds), and fraction6(aromatic compounds) were next order. These results were confirmed by morphological examination of VSMCs. These results suggest that PM_(2.5) inhibits the DAN synthesis of VSMCs through the cytotoxicity.

Identification of QTL for grain quality traits using introgression lines derived from an interspecific cross in rice

Yeo-Tae Yun,Chong-Tae Chung,Yeong-Ju Lee,Han-Jung Na,Jae-Chul Lee,Kwang-Won Lee,Young-Hwan Yoon,Ju-Won Kang,Hyun-Sook Lee,Sang-Nag Ahn 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07

96 BC3F5 introgression lines derived from a cross between Hwaseong and O. rufipogon were genotyped with 131 simple sequence repeat (SSR) markers to identify and characterize quantitative traits loci (QTLs) associated with grain quality traits in rice. 96 BC3F5 lines displayed a wide range of variation for days to heading and agronomic traits. Results indicated that one major QTL (qDTH6) on chromosome 6 was associated with significant variation for days to heading. 83 lines without the O. rufipogon segment at qDTH6 were selected and analyzed for grain quality traits. QTL analysis was conducted for two groups, 96 and 83 introgression lines, and a total of 25 QTLs were detected for rice quality traits. 16 QTLs were detected in a group of 93 lines, 11 QTLs detected in a group of 83 lines, and 2 QTLs were commonly identified in both groups. Most of the QTLs detected in this study were located on the same or adjacent regions as those reported by the previous studies, and the wild alleles negatively affected quality traits. In contrast, the wild allele at qGCR9 for the glossiness of cooked rice on chromosome 9 contributed to an increase in glossiness which is positively correlated with rice eating quality. Three ILs with the wild allele at qGCR9 displayed better eating quality than the recurrent parent, Hwaseong. To confirm the effect of qGCR9, high density mapping of the qGCR9 with a series of NILs will be conducted.

EFFECTS OF DIETARY AFLATOXIN B1 ON PERFORMANCE, ON HEMATOLOGIC, PATHOLOGIC AND IMMUNOLOGIC CHANGES IN BROILER CHICKENS

Yun, Yeo-Pyo,Kim, Kan-Hol,Han, Sang-Bae,Chung, Chung-Soo,Jeong, Goo-Bo Korean Society of ToxicologyKorea Environmental Mu 1992 Toxicological Research Vol.8 No.1

The influences of dietary aflatoxin B1 on performance, on hematologic, pathologic and immunologic changes in broiler chickens were studied. One hundred and fifty hatched broiler chickens were fed with diet containing aflatoxin B1 (1.0 ppm and 2.5 ppm) fot three weeks. Blood samples, serum, and immune organs were obtained to investigate hematological, clinico-chemical, and histopathologial changes. Body weight gain and feed intake were significantly decreased. The liver and kidney were increased, whereas the bursa of Fabricius, spleen and thymus were decreased.

6-(3,4-디클로로페닐)아미노-7-클로로-5,8-퀴놀린디온의 항진균작용 및 안전성 평가

윤여표(Yeo pyo Yun),김동현(Dong Hyun Kim),이병무(Byung Mu Lee),허문영(Moon Young Heo),정해문(Hae Moon Chung),강혜영(Hye Young Kang),최정아(Jung Ah Choi),김도희(Do Hee Kim),유충규(Chung Kyu Ryu) 대한약학회 1998 약학회지 Vol.42 No.5

6-(3,4-Dichlorophenyl)amino-7-chloro-5,8-quinolinedione (RCK50) was tested for antifungal activities in mice systemically infected with Candida albicans. The therapeutic potential of RCK50 was also assessed in comparison with ketoconazole. CK50 had ED50 0.22 +/- 0.01mg/kg. Ketoconazole as a positive control had ED50 6.00 +/- 1.70mg/kg. Intraperitoneally administered RCK50 at the ED50 for 7 days and 14 days reduced Candida albicans colony count in the kidneys and liver. And administered RCK50 at the ED50 for 14 days improved survival rates. The genotoxicities of RCK50 had been evaluated. RCK50 was negative in Ames test with Salmonella typhimurium and chromosomal aberration test in CHL cells. RCK50 did not show any clastogenic effect in mouse peripheral blood and was negative in mouse micronucleus assay. These results indicate that RCK50 has no genotoxic potential under these experimental conditions. Acute oral toxicity studies of RCK50 were carried out in ICR mice of both sexes. RCK50 did not show acute oral toxicities and LD50 values were over 2,850mg/kg in ICR mice.

Mapping quantitative trait loci for agronomic traits using introgression lines carrying wild rice (Oryza rufipogon) chromosome segments in cultivar background

Sang-Min Yeo,Yeo-Tae Yun,Chong-Tae Chung,Jung-Phil Seo,Hae-Hwang Kim,Sang-Nag Ahn 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The objective of this study were to identify QTLs for agronomic traits using a set of introgression lines carrying wild rice (Oryza rufipogon) segment in cultivated rice (ssp. japonica cv. Hwaseongbyeo). Ninety-six ILs were evaluated for seven agronomic traits, amylose and protein contents. The proportion of the recurrent genome in ILs ranged from 87.8 to 100%, with an average of 96.7%. The mean number of homozygous and heterozygous donor segments were 2 (ranging 0-7) and 1.7 (ranging 0-6), respectively, and the majority of these segments had size less than 10 cM. A total of 22 quantitative trait loci were identified for 9 traits and each QTL explained 7.2% to 56.6% of the phenotypic variance. Some QTLs were clustered in a few chromosomal regions. A first cluster was located near RM527 on chromosome 6 with QTLs for culm length, panicle length, days to heading, 1000-grain weight and protein content. Three ILs with high spikelets per panicle compared to the recurrent parent were selected to detect and fine map the wild segments responsible for this variation. The results will be discussed.

Effect of Pyridoxine on Rifampicin Toxicity

Yun, Yeo-Pyo,Kim, Koan-Hoi,Kim, Hack-Seang,Chung, Jin-Ho The Pharmaceutical Society of Korea 1991 Archives of Pharmacal Research Vol.14 No.1

The effects of pyridoxine (PN) on rifampicin (RMF) toxicity were investigated by both in vivo and in vitro methods. RMF (30 mg/kg) was injected intraperitoneally and PN(150 mg/kg) was administered orally to rats for 10 consecutive days. After treatment, clinical chemistry and hematologic profiles were measured. RMF and PN plus RMF did not show any adverse effects at this in vivo experimental condition. Thymidine incorporations of mice bone marrow cells were examined in vitro. RMF showed a decrease in thymidine uptake in a dose-dependent manner, but PN showed a reversal of the thymidine uptake suppression caused by RMF (p<0.01). On the other hand, PN showed a decrease in thymidine uptake at a high concentration level.