아이스박스를 타고 온 천사

윤보라 샘터사 2013 월간 샘터 Vol.516 No.-

Colorimetric Detection of Fatty Amines Based on Conjugated Polydiacetylenes

윤보라,김광환,김종만 한국고분자학회 2011 한국고분자학회 학술대회 연구논문 초록집 Vol.2011 No.1

Inkjet Printing of Polydiacetylene Microemulsions on Paper Substrates

윤보라,신효라,김종만 한국고분자학회 2012 한국고분자학회 학술대회 연구논문 초록집 Vol.2012 No.4

백출의 Atractylenolide I과 Atractylenolide III의 함량분석

윤보라,마충제,원진배,이보형,이지우,엄민례 한국생약학회 2013 생약학회지 Vol.44 No.1

This study was conducted to determine the content of atractylenolides I and III in Atractylodes japonica. An established HPLC-DAD method was used to monitor contents of atractylenolides I and III in A. japonica obtained from Korea and China and compared with contents of A. macrocephala, A. chinensis, and A. lancea. Quantitative analysis of atractylenolides I and III was carried out on a Shiseido C18 column (S-5 µm, 4.6 mm I.D. × 250 mm) with gradient elution composed of acetonitile-water. The results show that the average content of atractylenolides I and III in A. japonica were 0.0954 and 0.1963%,respectively, and contents of A. lancea were higher than A. macrocephala, A. chinensis. In this study, we identified the differentiation of the quality of A. japonica from different species and collected locations and established content standard of atractylenolides I and III in A. japonica and this content standard was helpful to quality control of A. japonica.

류마티스 관절염에서 Glutathione S-transferase 유전자 다형성에 관한 연구

윤보라,AhmedEl-Sohemy,MarilynC.Cornelis,곽은주,강태영,정청일,이혜순,엄완식,김태환,전재범,유대현,배상철 대한류마티스학회 2003 대한류마티스학회지 Vol.10 No.3

Association of Polymorphism in Glutathione S-transferase Gene with Susceptibility and Severity of Rheumatoid ArthritisBo-Ra Yun, M.D., Ahmed El-Sohemy, Ph.D.*, Marilyn C. Cornelis, M.S.*,Eun-Joo Kwak, M.S., Tae-Young Kang, M.D., Chung-Il Joung, M.D.,Hye-Soon Lee, M.D., Wan-Sik Uhm, M.D., Tae-Hwan Kim, M.D.,Jae-Bum Jun, M.D., Dae-Hyun Yoo, M.D., Sang-Cheol Bae, M.D.Center, Seoul, Korea,

Intratumoral Heterogeneity of Breast Cancer Xenograft Models: Texture Analysis of Diffusion-Weighted MR Imaging

윤보라,조나리야,Mulan Li,장민혜,박소연,강호철,김보형,송인찬,문우경 대한영상의학회 2014 Korean Journal of Radiology Vol.15 No.5

Objective: To investigate whether there is a relationship between texture analysis parameters of apparent diffusion coefficient (ADC) maps and histopathologic features of MCF-7 and MDA-MB-231 xenograft models. Materials and Methods: MCF-7 estradiol (+), MCF-7 estradiol (-), and MDA-MB-231 xenograft models were made with approval of the animal care committee. Twelve tumors of MCF-7 estradiol (+), 9 tumors of MCF-7 estradiol (-), and 6 tumors in MDA-MB-231 were included. Diffusion-weighted MR images were obtained on a 9.4-T system. An analysis of the first and second order texture analysis of ADC maps was performed. The texture analysis parameters and histopathologic features were compared among these groups by the analysis of variance test. Correlations between texture parameters and histopathologic features were analyzed. We also evaluated the intraobserver agreement in assessing the texture parameters. Results: MCF-7 estradiol (+) showed a higher standard deviation, maximum, skewness, and kurtosis of ADC values than MCF-7 estradiol (-) and MDA-MB-231 (p < 0.01 for all). The contrast of the MCF-7 groups was higher than that of the MDA-MB-231 (p = 0.004). The correlation (COR) of the texture analysis of MCF-7 groups was lower than that of MDA-MB-231 (p < 0.001). The histopathologic analysis showed that Ki-67mean and Ki-67diff of MCF-7 estradiol (+) were higher than that of MCF-7 estradiol (-) or MDA-MB-231 (p < 0.05). The microvessel density (MVD)mean and MVDdiff of MDA-MB-231 were higher than those of MCF-7 groups (p < 0.001). A diffuse-multifocal necrosis was more frequently found in MDA-MB-231 (p < 0.001). The proportion of necrosis moderately correlated with the contrast (r = -0.438, p = 0.022) and strongly with COR (r = 0.540, p = 0.004). Standard deviation (r = 0.622, r = 0.437), skewness (r = 0.404, r = 0.484), and kurtosis (r = 0.408, r = 0.452) correlated with Ki-67mean and Ki-67diff (p < 0.05 for all). COR moderately correlated with Ki-67diff (r = -0.388, p = 0.045). Skewness (r = -0.643, r = -0.464), kurtosis (r = -0.581, r = -0.389), contrast (r = -0.473, r = -0.549) and COR (r = 0.588, r = 0.580) correlated with MVDmean and MVDdiff (p < 0.05 for all). Conclusion: The texture analysis of ADC maps may help to determine the intratumoral spatial heterogeneity of necrosis patterns, amount of cellular proliferation and the vascularity in MCF-7 and MDA-MB-231 xenograft breast cancer models.

Radiofrequency Ablation for Treating Liver Metastases from a Non-Colorectal Origin

윤보라,이정민,백지현,김세형,이재영,한준구,최병인 대한영상의학회 2011 Korean Journal of Radiology Vol.12 No.5

Objective: We wanted to assess the safety and efficacy of performing radiofrequency ablation (RFA) in patients with noncolorectal liver metastases. Materials and Methods: In this retrospective study, 25 patients with 40 hepatic metastases (M:F = 17:8; mean age, 57years; tumor size, 0.5-5.0 cm) from a non-colorectal origin (stomach, biliary, breast, pancreas, kidney and skin) were treated with RFA. The RFA procedures were performed using either an internally cooled electrode or a clustered electrode under ultrasound or CT guidance. Contrast-enhanced CT scans were obtained immediately after RFA and follow-up CT scans were performed within three months after ablation and subsequently at least every six months. The intrahepatic diseasefree interval was estimated and the overall survival from the time of the initial RFA was analyzed using the Kaplan-Meier method. Results: No intraprocedural deaths occurred, but four major complications developed, including abscesses (n = 3) and pneumothorax (n = 1). Technical effectiveness was determined on the initial follow-up images. During the follow-up period (range, 5.9-68.6 months; median time, 18.8 months) for 37 tumors in 22 patients where technical effectiveness was achieved, 12 lesions (32%, 12 of 37) showed local tumor progression and new intrahepatic metastases occurred in 13patients (59%, 13 of 22). The median intrahepatic disease-free interval was 10.1 months. The 1-year, 3-year and 5-year overall survival rates after RFA were 86%, 39% and 19%, respectively. Conclusion: RFA showed intermediate therapeutic effectiveness for the treatment of non-colorectal origin liver metastases.

주관적기억장애 환자의 미래계획기억: 예비 연구

윤보라,정성우,심용수 대한치매학회 2008 Dementia and Neurocognitive Disorders Vol.7 No.1

Background: Subjective memory impairment (SMI), defined by memory complaints with normal age-, gender- and education-adjusted cognitive performance, is a frequent phenomenon in elderly people. According to longitudinal studies, SMI is probably a predictor for future mild cognitive impairment (MCI) and dementia. However, there has been no objective neuropsychological test for evaluating SMI, and previous studies relied solely on patients’ subjective complaints. Thus, our goal was to make an objective standard that is useful in distinguishing SMI from normal aging as well as MCI by considering prospective memory (PM) as a higher level of memory. Methods: The study participants included seven healthy controls, 25 individuals with SMI and eight patients with amnestic MCI (aMCI). The inclusion criteria for SMI were 1) over 55 yr, 2) sustained subjective memory complaints, 3) normal general cognition, 4) no abnormality (within-1SD) in neuropsychological battery and 5) no depression. aMCI was followed by Pitersen’s criteria. To assess prospective memory, the Cambridge Behaviour Prospective Memory Test, which consisted of four time-based and four event-based tasks, was performed. Subsequently, scores from Korean Mini-mental Status Examination (K-MMSE), the 20 min delayed recall Seoul verbal learning test (SVLT), and individual task scores of PM and total PM scores were compared among each group. Results: There were no significant group differences in age, education, sex, K-MMSE score and time-based PM among the three groups. However, SVLT scores and total PM scores showed a significant decline in the aMCI group, unlike that for the SMI and control groups. Moreover, eventbased PM score was significantly decreased stepwise in both SMI and aMCI groups. Conclusions: These findings suggest that PM, especially for event-based tasks, is sensitive for detecting the earliest cognitive changes associated with SMI, and PM tasks seem to be more susceptible to the early cognitive decline than retrospective memory tasks. As PM is a sensitive predictor for future cognitive decline, SMI may be a first manifestation of future dementia in the elderly regardless of depression. Future research will help determine the clinical usefulness of PM tasks. Background: Subjective memory impairment (SMI), defined by memory complaints with normal age-, gender- and education-adjusted cognitive performance, is a frequent phenomenon in elderly people. According to longitudinal studies, SMI is probably a predictor for future mild cognitive impairment (MCI) and dementia. However, there has been no objective neuropsychological test for evaluating SMI, and previous studies relied solely on patients’ subjective complaints. Thus, our goal was to make an objective standard that is useful in distinguishing SMI from normal aging as well as MCI by considering prospective memory (PM) as a higher level of memory. Methods: The study participants included seven healthy controls, 25 individuals with SMI and eight patients with amnestic MCI (aMCI). The inclusion criteria for SMI were 1) over 55 yr, 2) sustained subjective memory complaints, 3) normal general cognition, 4) no abnormality (within-1SD) in neuropsychological battery and 5) no depression. aMCI was followed by Pitersen’s criteria. To assess prospective memory, the Cambridge Behaviour Prospective Memory Test, which consisted of four time-based and four event-based tasks, was performed. Subsequently, scores from Korean Mini-mental Status Examination (K-MMSE), the 20 min delayed recall Seoul verbal learning test (SVLT), and individual task scores of PM and total PM scores were compared among each group. Results: There were no significant group differences in age, education, sex, K-MMSE score and time-based PM among the three groups. However, SVLT scores and total PM scores showed a significant decline in the aMCI group, unlike that for the SMI and control groups. Moreover, eventbased PM score was significantly decreased stepwise in both SMI and aMCI groups. Conclusions: These findings suggest that PM, especially for event-based tasks, is sensitive for detecting the earliest cognitive changes associated with SMI, and PM tasks seem to be more susceptible to the early cognitive decline than retrospective memory tasks. As PM is a sensitive predictor for future cognitive decline, SMI may be a first manifestation of future dementia in the elderly regardless of depression. Future research will help determine the clinical usefulness of PM tasks.

A Survey on Current Trends of Breast Imaging Practices in Korea

윤보라,김선미,장미정,조나리야,한부경 대한영상의학회 2019 대한영상의학회지 Vol.80 No.5

Purpose This study aimed to analyze the current practices of breast imaging among Korean radiologists. Materials and Methods We invited members of the Korean Society of Breast Imaging (KSBI) working as breast radiologists to participate in a survey on the current practices in breast imaging, and investigated how quality assurance (QA), medical audits, and imaging-histologic discordance were conducted. Results The survey involved 312 members of the KSBI, and 116 (37%) responded to the 21 questions in the questionnaire. Most of the respondents were women (87%), aged below 50 years (82.7%), and working at university or tertiary hospitals (68.1%), who had varying levels of experience in breast imaging. Of the 116 respondents, 114 (96.6%) followed the Breast Imaging Reporting and Data System (BI-RADS) to interpret breast imaging. Out of 116, 72 (62.1%) interpreted 20 or more mammograms per day, 84 (72.4%) performed 10–30 breast ultrasound scans per day, and 65 (56%) interpreted fewer than 5 breast MRI scans per day. Among the respondents, 82% performed mammography QA, 76.9% evaluated imaging-histologic correlations, and only 38.9% performed medical audits. Conclusion The institutions and working patterns of breast radiologists were diverse. Although many respondents did not conduct medical audits, most of them followed BI-RADS when interpreting breast imaging, performing QA, and evaluating imaging-histologic correlations.

Factors Associated with Lung Function Recovery at the First Year after Lung Transplantation

윤보라,박지은,김치영,박무석,김영삼,정경수,송주한,백효채,이진구,김송이 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.9

Purpose: Post-operative pulmonary function is an important prognostic factor for lung transplantation. The purpose of this studywas to identify factors affecting recovery of forced expiratory volume in 1 second (FEV1) at the first year after lung transplantation. Materials and Methods: We retrospectively reviewed the medical records of lung transplantation patients between October 2012and June 2016. Patients who survived for longer than one year and who underwent pulmonary function test at the first year of lungtransplantation were enrolled. Patients were divided into two groups according to whether they recovered to a normal range ofFEV1 (FEV1 ≥80% of predicted value vs. <80%). We compared the two groups and analyzed factors associated with lung functionrecovery. Results: Fifty-eight patients were enrolled in this study: 28 patients (48%) recovered to a FEV1 ≥80% of the predicted value, whereas30 patients (52%) did not. Younger recipients [odds ratio (OR), 0.92; 95% confidence interval (CI), 0.87–0.98; p=0.010], longerduration of mechanical ventilator use after surgery (OR, 1.14; 95% CI, 1.03–1.26; p=0.015), and high-grade primary graft dysfunction(OR, 8.08; 95% CI, 1.67–39.18; p=0.009) were identified as independent risk factors associated with a lack of full recovery oflung function at 1 year after lung transplantation. Conclusion: Immediate postoperative status may be associated with recovery of lung function after lung transplantation.