가스 원격검침 단말기(통신중계기) 개발

오기석(Ki-Suk Oh),이정한(Jung-Han Lee),손현익(Hyun-Ik Son) 한국가스학회 2022 한국가스학회 학술대회논문집 Vol.2022 No.11

국내공항 사례를 통한 디아이싱 시설 설치기준 연구

오기석(Oh Ki Suk),유승권(Yoo Seung Gwon) 대한토목학회 2003 대한토목학회 학술대회 Vol.2003 No.10

자궁경부암 환자에서 종양표지물질 CYFRA 21-1과 SCC Ag의 치료전 혈청치의 유용성

오기석(Kie Suk Oh),정태영(Tai Young Chung),정두용(Do Young Chung),유희석(Hee Sug Ryu),장기홍(Ki Hong Chang),전미선(Mi Son Chun),박찬희(Chan Hee Park),박영한(Young Han Park) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.12

목적: 폐(lung)의 비소세포암(non-small cell cancer) 뿐만 아니라 편평상피암의 진단에 사용되고 있는 CYFRA 21-1의 혈청치가 자궁경부암의 추적관찰 및 재발암을 예측하는데 있어 혈청종양표지물질로서 가장 널리 사용되고 있는 Squamous cell carcinoma antigen(SCC Ag)과 더불어 임상병리학적 변수로서의 부가적 가치에 대한 여부를 알아보고자 하였다. 연구방법: 1995년 11월부터 1997년 7월까지 아주대학교병원 산부인과에 내원한 중에서 CYFRA 21-1과 SCC Ag을 측정한 80명의 자궁경부암 환자와 정상 또는 부인과 양성질환자 59명을 대조군으로 후향적 연구를 하였다. 결과: 1. ROC curve에 의한 혈청 SCC Ag과 CYFRA 21-1의 정상 cut-off value는 각각 1.94ng/ml, 3.11ng/ml였다. 2. Cut-off point에 의한 SCC Ag의 민감도, 특이도, 양성예측율, 음성예측율은 각각 55, 95, 97, 46%, CYFRA 21-1은 각각 45, 91, 87, 55% 였으며 두 물질의 병용측정시 민감도는 62%로 증가하였고 특이도, 양성예측율, 음성예측율은 각각 72, 75, 58% 였다. 3. FIGO stage Ib-IIa 의 SCC Ag과 CYFRA 21-1의 혈청치는 각각 2.2±3.9ng/ml, 2.5±3.6ng/ml 였으며 stage IIb-IV 는 각각 12.2±15.2ng/ml, 10.8±11.2ng/ml 였다(p<0.05). 4. 종양종괴의 크기가 4cm 이하인 경우 SCC Ag과 CYFRA 21-1의 혈청치는 각각 3.3±9.0ng/ml, 4.5±7.6ng/ml 였으며 4cm 보다 큰 경우는 각각 11.8±11.9ng/ml, 7.7±9.3ng/ml 였다(p<0.05). 결론: CYFRA 21-1의 혈청치의 측정은 자궁경부암을 추적관리하는데 있어 SCC Ag 보다 민감도는 낮으나 병행측정시 보다 높은 민감도를 보이며 자궁경부암의 병기, 종양의 크기 등을 예측하는데 있어서 부가적 가치가 있는 물질로 사용가능할 것으로 사료된다. 또한 치료 후 추적관찰 중 재발암을 예측하는데 있어서도 SCC Ag 단독측정보다 CYFRA 21-1와의 병행측정시 더 효용성이 있을 것으로 생각되지만 보다 많은 환자를 대상으로 장기간에 걸친 연구가 필요할 것으로 생각된다. Objectives: SCC Ag(Squamous cell carcinoma antigen) is so far the most useful tumor marker in assisting clinical diagnosis of cervical cancer and follow-up after therapy. Elevated levels of cytokeratin 19-fragments(CYFRA 21-1) have recently been detected in large proportion of patients with non small cell cancer of the lung, and in particular those with squamous cell carcinoma. This study is to assess the clinical efficacy of CYFRA 21-1 with SCC Ag as the clinicopathologic parameter in cervical cancer. Method: Retrospective analysis of the serum tumor markers CYFRA 21-1 & SCC Ag in eighty cervical cancer patients was performed. Results: Cut off values for SCC Ag & CYFRA 21-1 were 1.94 ng/ml, 3.11 ng/ml respectively. Using the cut-off point, the sensitivity, specificity, positive predictive value(PPV), and negative predictive value(NPV) of serum SCC were 55, 95, 97, 46%, respectively. Serum CYFRA 21-1 showed a sensitivity of 45%, specificity of 91%, PPV of 87%, and NPV of 55%. The combination of SCC and CYFRA 21-1 increased the sensitivity to 62%, with a specificity, PPV, and NPV of 72, 75, 58%. Serum levels of both markers were compared with tumor stage, lesion size and were significantly related. In FIGO stage Ib-IIa, the serum levels of SCC Ag & CYFRA were 2.2±3.9, 2.5±3.6 ng/ml and in FIGO stage IIb-IV, 12.2±15.2, 10.8±11.2 ng/ml. In ≤4cm of lesion size the serum levels of SCC Ag & CYFRA were 3.3±9.0, 4.5±7.6 ng/ml and in >4cm of lesion size, 11.8±11.9, 7.7±9.3 ng/ml. Conclusion: These data seems to show that serum CYFRA 21-1 may be of additional value in assessing the state of disease in some patients with cervical cancer. The prediction of recurrent cervical cancer with SCC Ag were improved by the combination with CYFRA 21-1 but further investigation is needed.

자궁경부암에서 접합분자 E - cadherin 의 발현

오기석(Kie Suk Oh),주희재(Hee Jae Joo),김선영(Sun Young Kim),유희석(Hee Sug Ryu),정태영(Tae Young Chung),장기홍(Ki Hong Chang) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.2

N/A Objective: The aim of the present study was to evaluate the E-cadherin expression in normal cervical epithelium, carcinoma in situ of the cervix, and invasive carcinoma of the cervix, and to define the role of E-cadlherin expression in tumor invasion with respect to clinicopathologic parameters. Methods: We conducted immunodetection of E-cadherin in 58 cases of cervical carcinoma using immunohistochemistry in formalin-fixed, paraffin-embbeded sections, Rusults: E-cadherin expression was different between normal cervical epithelium and carcinoma in situ of the cervix, and between normal cervical epithelium and invasive carcinoma of the cervix(p<0.05). However, there was no difference in E-cadherin expression between carcinoma in situ and invasive carcinoma of the cervix. In invasive cervical carcinomas, expression of E-cadherin and the intensity of cytoplasmic E-cadherin expression did not correlate with histologic type, lymphvascular space invasion, lymph node metastasis, and stage of disease. Conclusion: It is mncluded that expression of E-cadherin is related to tumor invasion in cervical tissues, but further studies with regard toE-cadherin/catenin/cytoskeleton complex are needed to clarify the prognostic role of E-cadherin with respect to clinicopathologic parameters in invasive cervical carcinoma,

임신 중 살충제 amitraz에 노출된 랫드의 모독성 평가

신진영,오기석,신동호,김성호,김형진,박승춘,이현숙,정문구,김종춘,Shin, Jin-Young,Oh, Ki-Suk,Shin, Dong-Ho,Kim, Sung-Ho,Kim, Hyoung-Chin,Park, Seung-Chun,Lee, Hyun-Sook,Chung, Moon-Koo,Kim, Jong-Choon 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4

The present study was carried out to investigate the potential adverse effects of amitraz on pregnant dams after maternal exposure during the gestational days (GD) 1 through 19 in Sprague-Dawley rats. The test chemical was administered orally to pregnant rats at dose levels of 0, 3, 10, or 30 mg/kg/ day. During the test period, clinical signs, mortality, body weights, food consumption, serum biochemistry, gross findings, organ weights and reproductive findings on GD 20 were examined. In the 30 mg/kg group, an increase in the incidence of abnormal clinical signs and death, a suppression in the body weight gain, and a decrease in the food consumption were observed. A decrease in the liver weight and increases in the kidneys, adrenal glands and heart weights were also found. Serum biochemical investigations revealed increases in the aspartate aminotransferase (AST), total bilirubin, and chloride. In addition, an increase in the fetal death and decreases in the litter size and fetal body weight were seen at caesarean section. Inthe 10 mg/kg group, an increase in the incidence of abnormal clinical signs, decreases in the food consumption and liver weight, increases in the total bilirubin and chloride, and a decrease in the fetal body weight were observed. There were no adverse effects on clinical signs, mortality, body weights, food consumption, serum biochemistry, gross findings, organ weights and reproductive findings in the 3 mg/kg group. Based on the results, it was concluded that the 19-day repeated oral dose of amitraz to pregnant rats caused increases in the clinical signs, kidneys, adrenal glands and heart weights, AST, total bilirubin and chloride and decreases in the body weight gain, food consumption and liver weight at the dose levels of above 10 mg/kg/day. Under the present experimental conditions, the no-observed-adverse-effect level (NOAEL) of amitraz was considered to be 3 mg/kg/day.

100 gm 경구 당부하검사 결과 하나만 비정상을 보인 임신의 양상

한기수(Ki Su Han),양성천(Seong Cheon Yang),김행수(Haeng Soo Kim),양정인(Jeong In Yang),이희종(Hee Jong Lee),임재현(Jae Hyun Lim),임윤경(Yun Kyoung Lim),오기석(Kie Suk Oh) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.10

N/A Objective : To evaluate the clinical outcomes of pregnancies showing one abnormal glucose tolerance test value. Method : We performed 50 gm glucose challenge test in 5,019 pregnant women at 24-28 weeks of gestation. In 1,170 women with plasma glucose levels over 130 mg/dL, 100 gm oral glucose tolerance tests (OGTTs) were performed at 28-32 weeks of gestation. In the 888 cases who were followed up, according to the National Diabetes Data Group (NDDG) criteria, 122 cases with one abnormal 100 gm OGTT value were divided into three groups (groups 1, 2, 3 : abnormal value after 1, 2, 3 hours, respectively). These were compared with 577 cases (control group) with normal 100 gm OGTT value, retrospectively. Result : The incidence of one abnormal glucose tolerance test value was 2.6%, and there were no cases where the fasting plasma glucose level only was elevated. The incidence (control, group 1, group 2, group 3 : 19.4%, 43.8%, 25.7%, 29.6%) of poor maternal outcomes which contain any one of preeclampsia, hydramnios, cesarean delivery for cephalopelvic disproportion or failure to progress or fetal distress was highest in group 1 (p=0.025). The incidence (15.8%, 43.1%, 14.3%, 21.1%) of poor perinatal outcomes which contain any one of fetal distress, Apgar score of 5 minute < 7, hypoglycemia, respiratory distress syndrome, small for gestational age, perinatal death was also highest in group 1 (p=0.009). Logistic regression analysis for poor maternal outcomes showed odds ratio of 2.83 (95% confidence interval 1.02-7.87) in group 1 and 2.08 (95% confidence interval 1.22-3.55) in group 3, and for poor perinatal outcomes odds ratio of 4.24 (95% confidence interval 1.02-17.52) in group 1 and 3.30 (95% confidence interval 1.45- 7.48) in group 3. Conclusion : Pregnancies complicated with one abnormal glucose tolerance test value, particularly the group showing abnormal glucose tolerance test value after 1 or 3 hour exhibited adverse maternal and perinatal outcomes.

임파선 전이 및 자궁방 침윤이 동반된 임상병기 IB 자궁경부암에서 Cyclooxygenase - 2 단백의 과발현

양현원(Hyun Won Yang),오기석(Kie Suk Oh),유희석(Hee Sug Ryu),정태영(Tae Young Chung),장기홍(Ki Hong Chang),권혁찬(Hyuck Chan Kwon),김명신(Myoung Shin Kim) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.3

N/A Objective: the enzymes cyclooxygenase(COX)-1 and -2 are necessary for the synthesis of prostaglandins. COX-2 is usually absent in normal cells and is upregulated and expressed as a product of the immediate early gene during inflammatory processes. In previous studies, the expression of COX-2 has been shown to be induced by prointlammatory cytockines, and suggestions have been made that overexpression of COX-2 supresses apoptosis and is directly related to tumor growth. We the authors have attempted to determine a relationship between the tumor invasion and metastasis of uterine cervical cancer and COX and apoptosis by comparing the protein expression of apoptosis and COX-I and COX-2 in tumor tissues confirmed with cytokeratin, and therefe determine the clinicopathologic risk factors. Materials and methods: The subjects were 18 patients who were FIGO stage IB uterine cervical cancer patients who underwent surgery at the Ajou University Medical Center. The 18 cases were comprised of 12 cases of squamous cell carcinoma, 3 cases each of adenocarcinoma and adenosquamous carcinoma. There were 9 cases with lymph node or prarametrial involvement and 13 cases with lymphvascular space involvement. All tissues obtained from the cases were subject to immunohistochemical staining for COX-1, -2 and TUNEL method for apoptosis detection, and the following results were obtained. Results: Tumor tissues confirmed by cytokeratin wae separated into tumor surface, tumor stroma, and invasion site portions, and in which increased apoptosis was observed in the tumor surface and tumor stmma, but not in the invasion sites. COX-2 expression was observed in all tumor tissues, which was especially strong in the tumor invasion site. Therefore, it is suggested that COX-2 expression may supress cell apoptosis at the site of tumor invasion. When COX-2 expression was investigated when the cases were divided into groups with regard to the presence or absence of lymph node or parametrial involvement, there was statistically significant (Mann-Whitney U test) COX-2 expression seen microscopically in the tumor stroma (p-value 0.028) and tumor invasion site (p-value 0.040) compared to the tumor surface (p-value 0.499). In other words, in surgically treated stage IB cervical cancer patients, COX-2 was significantly expressed when lymph node or parametrial involvement was present. Conclusions: These results suggest that the expression of COX-2 in stage IB cervical cancer patients may downregulate apoptosic processes and thus enhances tumor invasion and metastasis.

인간의 착상 기전을 연구하기 위한 3차원적 자궁내막 모델 확립

박동욱,양현원,권혁찬,장기홍,김세광,조동제,오기석,Park, Dong-Wook,Yang, Hyun-Won,Kwon, Hyuck-Chan,Chang, Ki-Hong,Kim, Sei-Kwang,Cho, Dong-Jae,Oh, Kie-Suk 대한생식의학회 1999 Clinical and Experimental Reproductive Medicine Vol.26 No.1

In order to study the implantation mechanism various methods for culture of endometrial cells in vitro have been attempted. However, a disadvantage is that primary cultures of stromal and epithelial cells do not have the ability to differentiate, and therefore cannot be reproduced in the same manner as in vivo endometrium. The object of this study is to establish a three dimensional culture of endometrial cells which are both morphologically and functionally identical to in vivo endometrium. Endometrial tissues obtained after hysterectomies were cut into thin slices and treated with collagenase and trypsin-EDTA. The stromal cells and the epithelial cells were separated by centrifugation and cultured for 24 hours in DMEM media containing 10% FCS, 100 nM progesterone, and 1 nM estradiol. The cultured stromal cells were mixed with collagen gel and solidified, after which it was covered with matrigel. Epithelial cells were inoculated on the top and then cultured for 3 days. The three dimensionally cultured endometrial cells were stained for integrin ${\alpha}1,\;{\alpha}4,\;{\beta}3$, and cyclooxygenase-l, -2 by immunohistochemistry, which all showed strong expression. The cultured epithelial cells showed the formation of microvilli, tight junctions and pinopodes by electron microscopy. Studies are currently under way utilizing this three dimensional culture model to ascertain the interaction between the embryo and human endometrial cells at the time of implantation, and it is thought that further studies into a new culture environment which would allow longer periods of culture will be necessary.

토종닭의 이면교배조합 시험을 이용한 신품종 종계 개발

손시환(Sea Hwan Sohn),최은식(Eun Sik Choi),김기곤(Ki Gon Kim),박병호(Byeongho Park),추효준(Hyo Jun Choo),허정민(Jung Min Heo),오기석(Ki Suk Oh) 한국가금학회 2021 韓國家禽學會誌 Vol.48 No.2

본 연구는 생산능력이 우수한 신품종 토종 종계를 개발하기 위하여 ㈜한협원종이 보유한 원종계(GPS) 4개 계통 934수를 이용하여 4 × 4 이면교배조합(diallel cross-mating) 검정 시험을 실시하였다. 검정 형질로서 외모 형태, 생존율, 체중, 초산일령, 난중 및 산란율을 대상으로 조합별 생산능력, 조합가, 결합능력 및 상반교잡 효과를 분석하였다. 분석 결과, 대부분의 교배조합 개체들의 깃털 색은 황갈색 및 적갈색과 이들 색 간의 혼합 양상을 나타내었다. 전체 조합의 평균 생존율은 86.8±12.3%이고, YH 조합의 생존율이 가장 높았다. 생존율의 조합가는 특정결합능력이 일반결합능력에 비해 상대적으로 크게 영향을 미치는 것으로 나타났고, HY의 특정결합능력이 가장 높게 나타났다. 체중의 경우, 16개의 교배 조합이 유전적 특성에 따라 세 그룹으로 명확히 구분되는 것으로 나타났다. 12주령 체중의 교배조합별 조합가는 계통별 일반결합능력이 두 계통 간의 특정결합능력보다 크게 영향을 미치는 것으로 나타났고, FH 조합에서 가장 높은 조합가가 추정되었다. 산란 형질에 있어 전체 교배조합구의 평균 초산일령은 157일로서 교잡 구가 순계 구보다 빠른 성 성숙을 보였다. 전체 조합의 평균 일계산란율은 69.0±10.9%로서 이들 중 SY 조합이 가장 높은 산란율을 보였다. 산란율의 일반결합능력과 특정결합능력의 추정 범위가 거의 비슷한 값을 나타내고 있으며, 교배조합 간 특정결합능력은 HS 및 FY조합에서 높게 나타났다. 또한 상반교잡 효과는 대부분의 조합에서 S와 Y를 모(母)로 사용하였을 때 자손들의 산란능력이 상대적으로 높게 나타났다. 이상의 결과에 따라 신품종 토종닭 종계로써 부계는 체중이 우수하면서 상대적으로 생존율이 양호한 FH나 HF 조합이, 모계는 산란능력이 우수하며 적절한 체중을 지닌 FY, FS, HY 및 SY 조합이 바람직한 것으로 사료된다. We conducted a 4 × 4 diallel cross-mating test using 934 chickens from four grandparent stock lines to develop a new synthetic breed of Korean native chicken. The mean values, combining ability, and reciprocal effects on survival rate, body weight, and hen-day egg production were analyzed. In phenotypes, most chickens have yellowish-brown, reddish-brown and mixed color feathers. The average survival rate was 86.8±12.3%, with the highest in YH combination. Specific combining ability (SCA) had a greater effect on survival rate than general combining ability (GCA), and the SCA of HY combination was the highest. The 16 cross-combinations were distinctly divided into three weight groups according to their genetic characteristics. At 12 weeks of age, GCA showed a greater effect on weight than SCA, and the SCA of FH combination was the highest. The age at first egg laying was 157 days, and the crosses reached sexual maturity faster than the pure lines. The egg production rate was highest in SY at 79.5±2.1%. The GCA and SCA for hen-day egg production were similar, and the SCA was highest in the HS and FY combinations. The reciprocal effect showed that the offspring’s egg production rate was high when S and Y were maternal parents in almost all combinations. In conclusion, FH and HF combinations, which have excellent growth performance, are the most desirable paternal parent stock strains, and FY, FS, HY, and SY combinations, which have excellent laying performance with moderate weight, are the preferred maternal strains.

임신과 동반된 유방암

변영지 ( Young Ji Byun ),양정인 ( Jeong In Yang ),유희석 ( Hee Sug Ryu ),오기석 ( Ki Suk Oh ),박희붕 ( Hee Boong Park ),임현이 ( Hyun Ee Yim ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.9

The breast cancer is the second most common malignancy in pregnancy after cervical cancer. Pregnancy-associated breast caner tends to show unfavorable prognosis, because it is diagnosed at more advanced stage due to delay of diagnosis and treatment. For a