4차 산업의 핵심기술인 빅데이터 이용 신약개발 전략

여인준,홍진태 한국에프디시규제과학회(구 한국에프디시법제학회) 2017 FDC법제연구 Vol.12 No.2

Digital Foreground Self-Calibration of Capacitor Mismatch for SAR ADCs

여인준,이병근 한국과학기술원 반도체설계교육센터 2016 IDEC Journal of Integrated Circuits and Systems Vol.2 No.3

This paper presents foreground digital self- calibration technique for successive approximation resistor (SAR) analog to digital converters (ADCs) is proposed and verified with simulations. Recent advanced in complementary metal oxide semi-conductor (CMOS) technologies and an asynchronous switching allows SAR ADCs to be utilized in a variety of applications. In SAR ADCs, the ADC linearity is mainly limited by capacitor random variation which heavily depends on integrated circuits(IC) processes. The proposed calibration technique effectively averaged out capacitor mismatch errors by taking advantages of anti-symmetric property of mismatch errors in SAR ADCs. This calibration technique significantly reduces capacitor mismatch errors without resorting extensive computation or dedicated circuits. A 12-bit 50 MS/s ADC fabricated in a 65nm CMOS process occupies a die area of 0.12 mm2. After calibration, a signal-to-noise distortion ratio (SNDR) and spurious-free dynamic range (SFDR) are improved from 44.12 dB to 72.85 dB and 50.59dB to 96.47dB, respectively.

Preparation of Tissue Samples for Large-scale Quantitative Mass Spectrometric Analysis

김요섭,여인준,Hyun Soo Kim,손민수,김영수 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.4

Tissues contain more tumor-type specific information than biofluids, such as blood, rendering them valuable resources for biomarker studies. However, considering the characteristics of tissue homogenization, it is difficult to obtain reproducible samples and analyze many samples simultaneously. To address these issues, we developed a robust and reproducible method for preparing tissues for targeted proteomics—multiple reaction monitoringmass spectrometry (MRM-MS)—using a Bioruptor Pico sonicator. This approach uses sodium deoxycholate (SDC) as a detergent and can extract proteins from up to 20 mg of tissue using a lysis buffer volume of 300 μL and a sonication time of 30 s, with 30 on/off cycles. The tryptic digestion was optimized as follows: digestion base buffer, ammonium bicarbonate (ABC); reduction and alkylation reagent, dithiothreitol (DTT) and iodoacetamide (IAA), respectively; and trypsin amount and incubation time, 1:50 (enzyme: substrate) and 10 h, respectively. With regard to reproducibility, the intra-assay and inter-assay CVs for the target peptides were less than 20% (intra-CV, 0.87% to 19.13%; inter-CV, 2.3% to 13.62%). Our method was robust and reproducible in the quantitative analysis of tissue by MRM-MS, rendering it applicable to the large-scale study of tissue-based biomarkers.

Isolation and Characterization of Pepsin-soluble Collagens from Bones, Skins, and Tendons in Duck Feet

김현욱,여인준,황고은,송동헌,김용재,함윤경,정태준,최윤상,김천제 한국축산식품학회 2016 한국축산식품학회지 Vol.36 No.5

The objectives of this study were conducted to characterize pepsin-soluble collagen (PSC) extracted from bones (PSC-B), skins (PSC-S), and tendons (PSC-T) of duck feet and to determine their thermal and structural properties, for better practical application of each part of duck feet as a novel source for collagen. PSC was extracted from each part of duck feet by using 0.5 M acetic acid containing 5% (w/w) pepsin. Electrophoretic patterns showed that the ratio between α and α chains, which are subunit polypeptides forming collagen triple helix, was approximately 1:1 in all PSCs of duck feet. PSC-B had slightly higher molecular weights for α and α chains than PSC-S and PSC-T. From the results of differential scanning calorimetry (DSC), higher onset (beginning point of melting) and peak temperatures (maximum point of curve) were found at PSC-B compared to PSC-S and PSC-T (p<0.05). Fourier transform infrared spectroscopy (FT-IR) presented that PSC-S and PSC-T had similar intermolecular structures and chemical bonds, whereas PSC-B exhibited slight difference in amide A region. Irregular dense sheet-like films linked by random-coiled filaments were observed similarly. Our findings indicate that PSCs of duck feet might be characterized similarly as a mixture of collagen type I and II and suggest that duck feet could be used for collagen extraction without deboning and/or separation processes.

Combination Effect of Titrated Extract of Centella asiatica and Astaxanthin in a Mouse Model of Phthalic Anhydride-Induced Atopic Dermatitis

박주호,여인준,장준성,김기천,박미희,이희봄,한상배,홍진태 대한천식알레르기학회 2019 Allergy, Asthma & Immunology Research Vol.11 No.4

Purpose: In our previous study, we demonstrated that both titrated extract of Centella asiatica (TECA) and astaxanthin (AST) have anti-inflammatory effects in a 5% phthalic anhydride (PA) mouse model of atopic dermatitis (AD). The increasing prevalence of AD demands new therapeutic approaches for treating the disease. We investigated the therapeutic efficacy of the ointment form of TECA, AST and a TECA + AST combination in a mouse model of AD to see whether a combination of the reduced doses of 2 compounds could have a synergistic effect. Methods: An AD-like lesion was induced by the topical application of 5% PA to the dorsal ear and back skin of an Hos:HR-1 mouse. After AD induction, TECA (0.5%), AST (0.5%) and the TECA (0.25%) + AST (0.25%) combination ointment (20 μg/cm2) were spread on the dorsum of the ear or back skin 3 times a week for 4 weeks. We evaluated dermatitis severity, histopathological changes and changes in protein expression by Western blotting for inducible nitric oxide synthase (iNOS), cyclocxygenase (COX)-2, and nuclear factor (NF)-κB activity. We also measured the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and immunoglobulin E (IgE) in the blood of AD mice by enzyme-linked immunosorbent assay (ELISA). Results: PA-induced skin morphological changes and ear thickness were significantly reduced by TECA, AST and TECA + AST treatments, but these inhibiting effects were more pronounced in the TECA + AST treatment. TECA, AST and the TECA+AST reatments inhibited the expression of iNOS and COX-2; NF-κB activity; and the release of TNF-α, IL-6 and IgE. However, the TECA+AST treatment showed additive or synergistic effects on AD. Conclusions: Our results demonstrate that the combination of TECA and AST could be a promising therapeutic agent for AD by inhibiting NF-κB signaling.

전기 자동차 고효율 BMS 구현을 위한 하이브리드 타입 Charge Equalizer

나경민(Kyungmin Na),여인준(Yeoin June),변영재(Franklin Bien) 한국자동차공학회 2012 한국자동차공학회 지부 학술대회 논문집 Vol.2012 No.5-1

Nowadays, electric vehicle(EV) or hybrid electric vehicle(HEV) usually adopts a lithium-ion battery string, the charge imbalance of each battery makes many problems such as reduction of life cycle and explosion. Hence, the charge equalization circuit is required to guarantee life time and safety. In charge equalization circuit, the cost of a cell voltage sensing module is relatively high, but the equalization performance gets lower in case module is removed. For higher equalization performance without the cell voltage sensing module, an automatic chare equalizer based on switch-capacitor is proposed. One pre-charged capacitor with average voltage of batteries is implemented from a unidirectional DC-DC converter. The charge equalization can be automatically achieved by periodic connection between battery cell and capacitor charged average voltage without voltage sensing module. By using pre-charged capacitor in switch-capacitor converter, the faster equalization time can be obtained. This paper presents the operational principles and design consideration of the proposed equalizer.

퍼지 AHP를 이용한 SCM 시스템 선정 모델

서광규(Seo Kwang-Kyu),여인준(Yeo, In-Joon),심상우(Shim, Sang-Woo),전한구(Jeon, Han-Koo) 한국산학기술학회 2006 한국산학기술학회 학술대회 Vol.- No.-

Supply Chain Management(SCM) system is a critical investment that can affect future competitiveness and performance of a company. When adopting a new SCM system, organizations experience increasing difficulty in decision making because information technology is changing so rapidly these days. Therefore, organizations have been looking for industry standards and proven methods of selection that they can utilize to choose the best SCM system. To select an optimum solution, we need to consider a number of different quantitative and qualitative factors such as cost, user interface and convenience, reference site, and so on. In this study, we propose a solution selection model of SCM systems using Fuzzy AHP to maximize the return on investment in information technology. The proposed model can systematically construct the objectives of SCM system selection to support the business goals.

Effects of Red and Green Glassworts (Salicornia herbacea L.) on Physicochemical and Textural Properties of Reduced-salt Cooked Sausages

김현욱,황고은,송동헌,김용재,함윤경,여인준,정태준,최윤상,김천제 한국축산식품학회 2014 한국축산식품학회지 Vol.34 No.3

This study was conducted to determine the effects of red and green glasswort on the physicochemical and textural prop-erties of reduced-salt cooked sausages. The control was formulated with 1.5% NaCl; then, three reduced-salt treatmentswere prepared, with 0.75% NaCl (RS), 0.75% NaCl+1.0% red glasswort (RSR) and 0.75% NaCl+1.0% green glasswort(RSG), respectively. The addition of glasswort within the added amount of 1% had no influence on the pH value of thereduced-salt cooked sausages, regardless of the glasswort type. In terms of color, RSG treatment conveyed a higher hueangle value than the RSR treatment (p<0.05). Increases in the protein solubility (total and myofibrillar proteins) and appar-ent viscosity of reduced-salt meat batter that were due to the addition of glasswort were observed; however, there were nodifferences according to the type of glasswort (p>0.05). Furthermore, the addition of glasswort, regardless of its type,resulted in decreased cooking loss, and increased emulsion stability. As a result, reduced-salt cooked sausages formulatedwith either red or green glasswort demonstrated similar textural properties to those of the control. In conclusion, the type ofglasswort within an added amount of 1% had no influence on the physicochemical and textural properties of reduced-saltcooked sausages, except for the color characteristics. In terms of color alteration by the addition of glasswort, the red glass-wort, which in comparison with the green glasswort could minimize the color changes of reduced-salt cooked sausages,might be an effective source for manufacturing meat products.

Effect of Ginger Extract and Citric Acid on the Tenderness of Duck Breast Muscles

Fu-Yi He,김현욱,황고은,송동헌,김용재,함윤경,김시영,여인준,정태준,김천제 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.6

The objective of this study was to examine the effect of ginger extract (GE) combined with citric acid on the tenderness of duck breast muscles. Total six marinades were prepared with the combination of citric acid (0 and 0.3 M citric acid) and GE (0, 15, and 30%). Each marinade was sprayed on the surface of duck breasts (15 mL/100 g), and the samples were marinated for 72 h at 4ºC. The pH and proteolytic activity of marinades were determined. After 72 h of marination, Warner Bratzler shear force (WBSF), myofibrillar fragmentation index (MFI), pH, cooking loss, moisture content, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and protein solubility were evaluated. There was no significant (p>0.05) difference in moisture content or cooking loss among all samples. However, GE marination resulted in a significant (p<0.05) decrease in WBSF but a significant (p<0.05) increase in pH and MFI. In addition, total protein and myofibrillar protein solubility of GE-marinated duck breast muscles in both WOC (without citric acid) and WC (with citric acid) conditions were significantly (p<0.05) increased compared to non-GE-marinated duck breast muscles. SDS-PAGE showed an increase of protein degradation (MHC and actin) in WC condition compared to WOC condition. There was a marked actin reduction in GE-treated samples in WC. The tenderization effect of GE combined with citric acid may be attributed to various mechanisms such as increased MFI and myofibrillar protein solubility.