5637 인체 방광암세포의 이동성과 침윤성에 미치는 황흑산(黃黑散)의 영향

심원석,김민서,박상은,최영현,홍상훈,Shim, Won-suk,Kim, Min-serh,Park, Sang-eun,Choi, Yung-hyun,Hong, Sang-hoon 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.1

Objectives: The purpose of this study was to identify the inhibitory effects of Hwangheuk-san (HHS), a Korean multi-herb formula comprising four medicinal herbs, on cell migration and invasion, two critical cellular processes that are often deregulated during metastasis, using the human bladder cancer 5637 cell line.Methods: Cell viability, motility, and invasion were assessed by 3-(4,5-dimethyl-2 thiazolyl)-2,5-diphnyl-2H-tetrazolium bromide (MTT), wound healing migration, and Transwell assays, respectively. Gene expression was detected by Western blot analysis. In addition, the activities of matrix metalloproteinases (MMPs) and the values for transepithelial electrical resistance (TER) were analyzed using a Gelatinase Activity Assay Kit and an Epithelial Tissue Voltohmmeter, respectively.Results: Our data indicated that within the concentration range that was not cytotoxic, HHS effectively inhibited the cell motility and invasiveness of 5637 cells. HHS markedly decreased the expression and activity of MMP-2 and MMP-9, which was associated with unregulation of tissue inhibitors of metalloproteinase (TIMP)-1 and TIMP-2. Further investigation revealed that phosphorylation of phosphatidylinositol 3-kinase (PI3K) and AKT was decreased in HHS-treated 5637 cells, and a PI3K/AKT inhibitor synergistically reduced the inhibition of migration and invasion and also inactivated MMP-2 and MMP-9. Moreover, HHS increased the tightening of tight junctions (TJs), which was demonstrated by an increase in the TER, and reduced the expression the levels of claudin family members (claudin-3 and -4), which are major components involved in the tightening of TJs.Conclusions: The present findings demonstrated that HHS attenuated the migration and invasion of bladder cancer 5637 cells by modulating the activity of the PI3K/Akt signaling pathway and also through TJ tightening.

도꼬마리 열매 복용으로 발생한 중독증상(中毒症狀)의 한방치료 1례

심원석,박상은,홍상훈,Shim, Won-suk,Park, Sang-eun,Hong, Sang-hoon 대한한방내과학회 2015 大韓韓方內科學會誌 Vol.36 No.1

This is a clinical report of one patient with mental disorientation, abnormal behavior, spasm, and other symptoms caused by poisoning with Xanthii Fructus, who recovered completely with treatment. The patient was treated with Korean medical treatments, such as herbal medicine and acupuncture. After treatment, various symptoms caused by the Xanthii Fructus poisoning disappeared completely and laboratory findings, such as liver function tests (aspartate transaminase and alanine transaminase), were also close to the normal ranges. This case shows that Korean medicine therapy can be applicable in treating various symptoms caused by Xanthii Fructus poisoning. It is considered that further research is needed to confirm the safe use of Xanthii Fructus.

Surgical Outcomes after Distal Suburethral Sling Procedures for Stress Urinary Incontinence in Aged Patients

심원석,이광염,김계환,정한,윤상진 대한비뇨의학회 2009 Investigative and Clinical Urology Vol.50 No.10

Purpose: There are few reports describing objective and subjective outcomes in aged patients undergoing distal urethral polypropylene sling (DUPS) and canal transobturator tape (canal TOT) placement, which make the submucosal mesh ‘tension-free’ state in the operating room. We evaluated surgical outcomes and subjective satisfaction in patients over 70 years of age who underwent DUPS and canal TOT for the treatment of stress urinary incontinence (SUI). Materials and Methods: We studied 29 consecutive patients over 70 years of age who underwent DUPS and canal TOT for genuine SUI between January 2006 and April 2008. Surgical outcomes were determined on the basis of hemoglobin change, operative time, hospital stay, and complications. Subjective satisfaction was determined on the basis of disease-specific quality of life, measured via the Incontinence Impact Questionnaire-short form (IIQ-7) and the Urogenital Distress Inventory-short form (UDI-6). Results: No intraoperative or major postoperative complications were reported. No concomitant procedures were performed, including cystocele repair (n=0) or rectocele repair (n=0). The mean IIQ-7 and UDI-6 scores both decreased significantly after DUPS and canal TOT. Moreover, 82% of patients reported no SUI symptoms under any circumstances, and 91% of patients reported being rarely or never bothered by SUI symptoms. Conclusions: The surgical outcomes and subjective satisfaction seen with DUPS and canal TOT in this study were superior to those seen in other studies. However, it is possible that this study overestimated the effects of DUPS and canal TOT in aged women because of the small study sample. Therefore, additional studies are needed. Purpose: There are few reports describing objective and subjective outcomes in aged patients undergoing distal urethral polypropylene sling (DUPS) and canal transobturator tape (canal TOT) placement, which make the submucosal mesh ‘tension-free’ state in the operating room. We evaluated surgical outcomes and subjective satisfaction in patients over 70 years of age who underwent DUPS and canal TOT for the treatment of stress urinary incontinence (SUI). Materials and Methods: We studied 29 consecutive patients over 70 years of age who underwent DUPS and canal TOT for genuine SUI between January 2006 and April 2008. Surgical outcomes were determined on the basis of hemoglobin change, operative time, hospital stay, and complications. Subjective satisfaction was determined on the basis of disease-specific quality of life, measured via the Incontinence Impact Questionnaire-short form (IIQ-7) and the Urogenital Distress Inventory-short form (UDI-6). Results: No intraoperative or major postoperative complications were reported. No concomitant procedures were performed, including cystocele repair (n=0) or rectocele repair (n=0). The mean IIQ-7 and UDI-6 scores both decreased significantly after DUPS and canal TOT. Moreover, 82% of patients reported no SUI symptoms under any circumstances, and 91% of patients reported being rarely or never bothered by SUI symptoms. Conclusions: The surgical outcomes and subjective satisfaction seen with DUPS and canal TOT in this study were superior to those seen in other studies. However, it is possible that this study overestimated the effects of DUPS and canal TOT in aged women because of the small study sample. Therefore, additional studies are needed.

휘안광의 부선특성에 관하여

박경호,오재현,곽창섭,심원석 연세대학교 산업기술연구소 1980 논문집 Vol.12 No.2

The authors investigated thoroughly the flotation characteristics of stibnite. The flotation behavior of this mineral was carried out by using modified hallimond tube with various xanthates as a collector and modifying agents such as copper sulphate, lead acetate, sodium sulfide, zinc sulphate, sodium cyanide, sodium sulfite and potassium dichromate. The main results obtained in this experiments are summarized as follows; (1) Stibnite shows that it has maximum floatability in pH range 4.0 to 8.0. (2) Stibnite is activated by copper sulphate and lead acetate. (3) Potassium dichromate is an effective depressing agents in stibnite-xanthate system in pH range 4.0 to 6.0. (4) In the pH range in which the zinc sulphate is preciptated by hydroxide, stibnite is depressed effectively (5) Stibnite is depressed by sodium sulfide in alkaline solution.

실활치의 교정적 치아이동시 치근 및 치주조직의 변화에 관한 실험적 연구

심원석(Won-Seop Shim),정규림(Kyu-Rhim Chung) 대한치과교정학회 1990 대한치과교정학회지 Vol.20 No.3

The objective of this experiment is to observe structural differences in the orthodontic movement of vital (control group) and devitalized (experimental group) teeth in the mongrel dogs. The 5 utilized dogs in this experiment were approximately 1 year of age and their average weight was about 12Kg. Endodontic therapy was performed on the 2nd premolars in upper & lower jaws of each animal under the general anesthesia by intravascular injection of 25mg/Kg of pentobarbital sodium. The canals of the teeth were obturated by using gutta percha in conjunction with root canal sealer (AH26 Densply). One of the roots in the 2nd premolars was hemisected to make an extraction space for the devitalized teeth to be moved. The edgewise technique was employed for the movement of the teeth. Orthodontic models and intraoral roentgenograms were taken before and after orthodontic tooth movement. The open coil springs (.010 x .040) were used at interbraket space in order to provide equal forces (75gm) between the teeth in each arch wire. After 13 weeks of active orthodontic tooth movement, dogs were sacrified and the experimental results were examined through the intraoral radiography, microscopic examination and scanning electromicroscopic examination at the root surface. From the results of the study, the following conclusions may be drawn: 1. The root resorption and cemental deposition were observed within the pressure and tension site in both group. 2. The root resorbed lacunae were observed in the cementum and/or into the dentin in both group. 3. The prominent osteoblastic activities were observed on the alveolar margin in the tension site in both group. 4. A few of blood vessels were observed in the pressure site, but also lots of blood vessels were observed in the tension site especially in the periphery of the alveolar bone in both group. 5. In the pressure site, resorbed lacunae were formed with deep and narrow cavity in the control group; the shallow and wide cavity in the experimental group. 6. In the pressure site, the repaired cementum or cementoid tissue was lined on cementum in the experimental group, but not in the control group. 7. There was no significant difference between external root resorption of endodontically and vital teeth when both were subjected to orthodontic forces.

대상포진 척수염으로 유발된 척수손상 환자의 한방치료 1례

김민서,심원석,박상은,홍상훈,Kim, Min-serh,Shim, Won-suk,Park, Sang-eun,Hong, Sang-hoon 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.3

Objectives: This is a clinical report of one patient with both lower limb paraparesis and numbness as well as sensory impairment caused by a T10 spinal cord injury due to varicella zoster myelitis.Methods: The patient with the spinal cord injury induced by varicella zoster myelitis was treated using Korean medical treatments such as herbal medicine (Palmijihwang-tanggami), acupuncture, and moxibustion.Results: After treatments, improvements in muscular strength and the sensory impairment of both lower limbs were observed as well as improvements in various side effects such as the debridement state and laboratory findings of urine analysis.Conclusion: Given these results, it is considered that Korean medical treatment is effective for patients with spinal cord injury due to varicella zoster myelitis.

4차 산업혁명기술과 감성을 접목한 지하철 대기시간의 스마트한 관리방안 연구

이은기,박장원,심원석,김유노,조강현,이정수,백수빈 서비스마케팅학회 2017 서비스마케팅학회 학술대회 발표논문집 Vol.2017 No.11

서울교통공사는 서울지하철 1~8호선을 운영하는 대한민국의 대표적인 대 중교통 운영기관으로서, 서울의 교통분담률 약 35%를 차지하고 있다. 2017년 5 월 31일, 서울시민의 기대와 우려 속에 기존의 서울메트로(1-4호선 운영)와 도시 철도공사(5-8호선)를 합병하여 새롭게 탄생한 기관으로 그 어느 때보다 지하철의 안전과 서비스에 대한 시민들의 기대치가 높아지고 있는 것이 현실이다. 시민들 의 기대치를 충족시키기 위해서는 막대한 재원이 투입되어야 하지만 운송원가의 70%수준에도 못 미치는 요금체계로 인해 재원의 조달에는 한계가 존재한다. 지하철의 안전과 서비스를 제고하기 위해서는 신형전동차 도입, 역사 냉 방화사업, 노후시설 개량, 엘리베이터, 무빙워크, 편의시설의 확충 등 하드웨어적 인 개선이 선행되어야 하지만 재원조달의 한계로 일시에 개선할 수는 없는 바, 여기서는 최소한의 비용과 창의적인 아이디어로 승객들의 불편을 최소화하고 서 비스 품질을 높일 수 있는 방안을 중심으로 검토해보고자 한다. 서울교통공사가 제공하는 것은 운수서비스로서 일반 상품과는 달리 저장 이 불가능하고, 생산과 동시에 소비되는 특성을 지니기 때문에 대부분의 경우에 서비스를 이용하는 과정에 기다려야 하는 경우가 발생한다. 즉, 캐노피를 통해 지 하철 역사에 들어오는 순간부터 게이트 통과, 승강장 대기, 전동차 내에서, 환승 통로에서, 화장실에서, 그리고 목적지에 도착해 외부로 나가는 순간까지 짧고 긴 기다림의 연속인 것이다.

백화사설초(白花蛇舌草), 산자고(山慈姑), 절패모(浙貝母)에 의한 MDA-MB-231 인체 유방암 세포에서의 항암 효과

진명호,박선영,강유경,심원석,허희수,홍상훈,박철,최영현,박상은,Jin, Myung-Ho,Park, Sun-Young,Kang, You-Gyung,Shim, Won-Suk,Hur, Hee-Soo,Hong, Sang-Hoon,Park, Cheol,Choi, Yung-Hyun,Park, Sang-Eun 대한한방내과학회 2014 大韓韓方內科學會誌 Vol.35 No.2

O. diffusa, C. appendiculata and F. thunbergii are reported to possess many pharmacological activities including anti-oxidant, anti-inflammatory, anti-hypertension, anti-diabetic and anti-cancer effects. However, their anti-cancer activities in human breast cancer have not been clearly elucidated yet. Objectives: In the present study, we compared the in vitro cytotoxic effects of single and complex treatment of O. diffusa, C. appendiculata and F. thunbergii in human breast cancer MDA-MB-231 cells. Methods: After we treated human breast cancer MDA-MB-231 cells with O. diffusa, C. appendiculata and F. thunbergii. we evaluated viability, growth inhibition, morphological changes, apoptotic body formation, measurement of the cell cycle and formation of DNA fragmentation of these cells. Results: We found that single treatment of O. diffusa and F. thunbergii could inhibit cell proliferation in human breast cancer MDA-MB-231 cells. However, complex treatment of O. diffusa, C. appendiculata and F. thunbergii had weak or no effect on the cell proliferation of MDA-MB-231 cells. The first, anti-proliferative effects of O. diffusa in MDA-MB-231 cells was associated with G2/M arrest of cell cycle and apoptotic cell death. The second, anti-proliferative effect of F. thunbergii in MDA-MB-231 cells was associated with apoptotic cell death. Conclusions: Taken together, these findings suggest that O. diffusa and F. thunbergii may be a potential chemotherapeutic agent for the control of human breast cancer cells, further studies will be needed to identify the molecular mechanisms.

방광 내 소세포암에 동반된 전립선 선암

최민호,박정원,이성훈,이한새,심원석,박호기,윤상진 대한비뇨의학회 2007 Investigative and Clinical Urology Vol.48 No.10

Postpartum Emphysematous Cystitis after Casarean Section

박호기,김계환,정한,윤상진,이민성,김태범,심원석,최인호,장주현 대한배뇨장애요실금학회 2009 International Neurourology Journal Vol.13 No.2

Emphysematous cystitis is a rare infectious condition of the urinary bladder. Underlying diabetes mellitus is present in over half of reported cases with women being affected twice as often as men. It also occurs in alcoholism, undernutrition, radiating and immunosuppressive treatments. We present a case of postpartum emphysematous cystitis after Casarean section (C-sec) in a young female without underlying disease.