치과용 임플란트 적용 비귀금속 코어와 관련된 전조골세포의 변화

박정현,강신영,김종우,김장주,김웅철,김지환,Park, Jung-Hyun,Kang, Seen-Young,Kim, Jong-Woo,Kim, Jang-Ju,Kim, Woong-Chul,Kim, Ji-Hwan 대한치과기공학회 2018 대한치과기공학회지 Vol.40 No.2

Purpose: The purpose of this study is to investigate the non-precious metal core materials used in the dental laboratory to fabricate the implant superstructure by CAD / CAM method. And to observe and compare the morphology and distribution of the osteoblasts in relation to implant osseointegration. Methods: In this study, the mandibular right first molar tooth model was selected as an international standard to produce a single core. Using this model, the impression was made with the silicone rubber, the tooth model was scanned, and a single core was designed and 5-axis milling was performed. The materials used were Cobalt-Chromium and Nickel-Chromium, and the cores for dental implant top structures were fabricated according to the procedures of the dental labs. After the fabrication, the marginal area of the core was separated and cell culture experiment was performed. The osteoblast cells used MC3T3-E1, which is currently widely used. For morphological analysis of osteoblasts, cells were posttreated and observed using CLSM (Confocal Laser Scanning Microscope) and compared. Results: The cell adhesion behavior of the specimen surface measured by CLSM was uniformly distributed in specimen A (Cobalt-Chromium) than in specimen B (Nickel-Chromium). The distribution and changes of the cells were different in the two specimens. Conclusion : It is possible to confirm that specimen A (Cobalt-Chromium) is suitable for the living body through adhesion and proliferation of osteoblasts related to implant osseointegration in the non-precious metal superstructure used after implantation. It is considered that it is preferable to use Co-Cr when fabricating the superstructure.

임플란트 완성 환자의 구강건강관련 삶의 질과 관련 요인

박정현,엄상화,권현숙,조갑숙,허승주,이소영,유병철,Park, Jung-Hyun,Urm, Sang-Hwa,Kwun, Hyeon-Sook,Cho, Gab-Suk,Heo, Seung-Ju,Lee, So-Young,Yu, Byeng-Chul 한국치위생학회 2011 한국치위생학회지 Vol.10 No.2

Objectives : This study was purposed to estimate the level of oral health quality of life and the relationship between health-related factors and oral health quality of life in patients with successful dental implants. Methods : This questionnaire survey was conducted during the period from December 2009 to March 2010 with 126 patients confirmed successful dental implants in Busan. The oral health related quality of life and oral health-related factors were estimated with OHIP-14 and 8 oral health characteristics, respectively. Data analysis was performed with descriptive analysis, t-test, ANOVA, multiple regression analysis using SAS(ver 9.1) program. Results : The score of oral health quality of life in patients with successful dental implants was $12.25{\pm}7.82$. The scores of subscale of the oral health quality of life were $1.24{\pm}1.35$ in social disability, $1.60{\pm}1.37$ in handicap, $1.60{\pm}1.44$ in psychological disability, $1.70{\pm}1.38$ in functional limitation, $1.81{\pm}1.35$ in physical disability, $2.14{\pm}1.46$ in physical pain, $2.17{\pm}1.46$ in psychological discomfort, respectively. The related factors of oral health quality of life in patients with successful dental implants were absence of other type prosthesis and experience of tooth brushing education. Conclusions : The oral health related quality of life in patients with successful dental implants was relatively good condition. Social supports and chances for high quality denture and tooth brushing educations are needed to improve oral health related quality of life in patients with dental implants.

Stem Cell Based Strategies for the Treatment of Degenerative Retinal Diseases

박정현,구승엽,조명수,이학섭,최영민,문신용,유형곤,Park, Jung-Hyun,Ku, Seung-Yup,Cho, Myung-Soo,Lee, Hak-Sup,Choi, Young-Min,Moon, Shin-Yong,Yu, Hyeong-Gon The Korean Society for Reproductive Medicine 2010 Clinical and Experimental Reproductive Medicine Vol.37 No.3

망막 질환에서의 줄기세포 치료는 이전까지 치료가 불가능하다고 여겨졌던 환자들에서 시력을 향상시킬 수 있는 가능성 때문에 주목 받고 있다. 본문에서는 망막 전구세포의 분화를 위해 사용되는 태아 줄기세포, 배아줄기세포 및 성체줄기세포 등 다양한 세포 종류와, 내인적, 외인적 인자 및 이식 방법에 대해 논의하였다. 망막색소상피세포뿐만 아니라 시각세포 전구체로 성공적으로 분화시킨 실험적 연구가 보고되고 있다. 줄기세포기반치료는 아직 한계가 있지만 망막 질환 환자에서 시력을 회복하기 위한 보다 근본적인 치료 방법으로 기대되고 있다. Stem-cell therapy has the potential to improve vision in patients with untreatable retinal disease. Various types of cell source including fetal, embryonic and adult stem cells, intrinsic and extrinsic factors for differentiation into retinal progenitors and transplantation mode were discussed in this review. Experimental approaches have successfully induced photoreceptor precursor cells and retinal pigment epithelium. Stem-cell-based therapy is a promising treatment to restore vision in patients with retinal disease, in spite of the challenges.

경북지역에서 파종시기가 춘파 귀리의 생육특성과 사료작물 종자생산에 미치는 영향

박정현,최광원,정기웅,조익환,Park, Joung-Hyun,Choi, Gwang-Won,Jung, Gi-Woung,Jo, Ik-Hwan 한국초지조사료학회 2016 한국초지조사료학회지 Vol.36 No.4

경북 지역에서 수요가 높은 사료작물 중 춘파 귀리(Avena sativa L., cv. High Speed)의 안정적인 종자생산을 위하여 2015년과 2016년 2년간 파종 시기별(2월 말일, 3월 10일, 20일)로 파종(130 kg/ha) 하였을 때 귀리의 생육 특성, 종자 생산성 및 사료가치를 조사하였다. 출수기, 개화기 및 성숙기는, 매년 2월 말일 파종구와 3월 10일 파종구가 1~2일 차이로 거의 비슷하였다. 2월 말일 파종의 경우, 귀리의 초장이 타 파종구 대비 유의적으로 길었고 (p<0.05) 수장(穗長)은 2016년 최대 1cm 유의적으로 길었으며 단위 면적($m^2$)당 수수(穗數)도 2015년 유의적으로 가장 많았다($416.7ea/m^2$). 이상, 파종 시기별 춘파 귀리는 2월 말 파종이 초장, 간장, 수장 및 단위 면적($m^2$)당 수수 등에서 비교적 양호한 생육을 보였다. 1수립수(穗粒數)는, 2월 말일 파종구가 2016년 및 두 해 평균 각각 56.3 및 63.3개로 유의적으로 많았다(p<0.05). 1수립중(穗粒重)도, 2월 말일 파종구가 2016년 및 두 해 평균 각각 1.72 및 2.00 g/spike로 유의적으로 높았다(p<0.05). 종실 수량의 경우, 2월 말일 파종구에서 3월 10, 20일 파종구 보다 높은 수준이었으며 두 해 평균 가장 높은 종자 생산성(6,243 kg/ha)을 보였다(p<0.05). 조단백질, 조지방, 조섬유, ADF, NDF 및 TDN 함량에서 파종기와 무관하게 귀리의 사료가치가 우수한 것으로 평가되며 이상의 결과로 볼 때, 경북 경주지역에서 춘파 귀리의 적절한 파종 시기는, 종자 생산성 면에서 2월 말일이 효과적이었다. The objective of this study was to efficiently produce forage crop seed of spring oat (Avena sativa L., cultivar High Speed) in Gyeongbuk area. Forage crop oats (130 kg/ha) were sown with a randomized block design. They were sown in triplicates in 2015 and 2016 using basal fertilizer of 70 kg N, 74 kg P, and 39 kg K/ha. Agronomic traits and seed production of oats were investigated to determine the influence of seeding dates (February 28/29th, March 10th, and March 20th). Heading, flowering, and maturing dates of these oats sown on February 28/29th and March 10th were almost close to each other with 1-2 days of difference for all test plots. For oats sown on the last day (28/29) of Feb., plant heights (111.6-122.6 cm) were significantly longer than those sown in March each year. In addition, panicle number ($416.7per\;m^2$) and panicle length (17.3 cm) of oats sown on the last day of February were also significantly (p < 0.05) larger than those of oats sown in March in 2015 and 2016, respectively. Based on these results, it is recommended to sow these oats on the last day of February to have better agronomic traits in terms of plant height, stem length, panicle length, and panicle number $per\;m^2$. Grain numbers (56.3 and 63.3) and kernel weights (1.72 and 2.00 g) per spike in plots of oats sown on the last day of February were also significantly (p < 0.05) larger than those of oats in other plots in 2016 and in the average of 2015~2016, respectively. The highest grain yield (6,243 kg/ha) was obtained when oats were sown on the last day of February. Feed value of harvested oats was good in terms of proximate compositions including fiber contents and TDN contents. In conclusion, sowing on the last day of February is the most effective way to increase seed production of spring oats in Gyeongbuk area of Korea.

남해안 어류 및 전복가두리양식장의 퇴적물-수층 경계면에서의 물질플럭스 비교: 현장배양과 실내배양실험 연구

박정현,조윤식,이원찬,홍석진,김형철,김정배,Park, Jung-Hyun,Cho, Yoon-Sik,Lee, Won-Chan,Hong, Sok-Jin,Kim, Hyung-Chul,Kim, Jeong-Bae 해양환경안전학회 2012 해양환경안전학회지 Vol.18 No.6

지속적인 양식활동과 어장환경 보호를 위해서는 연안해역과 양식어종마다 각기 다른 연안생태계의 물질순환에 대한 연구가 필요하며, 양식장아래 퇴적물-수층 경계면의 침강 용출 플럭스에 관한 정확한 산정이 중요하다. 이에 관한 연구방법 중 벤틱 챔버를 이용한 현장배양법과 코아 채니를 이용한 실내배양실험법으로 퇴적물 산소요구량과 용존무기질소 용출 플럭스를 비교하여 보았다. 통영 어류 가두리양식장, 여수 어류 가두리양식장, 완도 전복양식장에 대하여, 퇴적물 산소요구량을 측정하여본 결과, 현장배양법은 116, 34, $31\;mmol\;O_2\;m^{-2}\;d^{-1}$, 실내배양법은 52, 17, $15\;mmol\;O_2\;m^{-2}\;d^{-1}$이었고, 용존무기질소 용출 플럭스의 경우, 현장배양법은 7.18, 7.98, $1.78\;mmol\;m^{-2}\;d^{-1}$, 실내배양법은 3.33, 3.74, $1.96\;mmol\;m^{-2}\;d^{-1}$로, 현장배양법이 실내배양법보다 약 2배 높게 나타났다. 본 연구는 각 양식장에 따른 물질 플럭스를 살펴봄으로서, 양식장아래 퇴적물-수층 경계면에 대한 두 가지 물질수지 계산 방법의 특성을 비교하였다. It is necessary to study the material circulation of coastal ecosystem according to aquacultural activity in order to induce the sustainable production of aquaculture and the fishery environment for the useful use. Hence, it is essential to make an exact assessment for the sedimentation release flux at the sediment-water interface in the aquafarm. Sediment oxygen demand and dissolved inorganic nitrogen release fluxes were compared using in-situ and laboratory incubational examination. Sediment oxygen demands were 116, 34, and $31\;mmol\;O_2\;m^{-2}\;d^{-1}$ (in-situ incubation), 52, 17, and $15\;mmol\;O_2\;m^{-2}\;d^{-1}$ (Core incubation) and dissolved inorganic nitrogen release fluxes were 7.18, 7.98, and $1.78\;mmol\;m^{-2}\;d^{-1}$ (in-situ incubation), 3.33, 3.74, and $1.96\;mmol\;m^{-2}\;d^{-1}$ (Core incubation) at Tongyeong finfish, Yeosu finfish, and Wando abalone cage farms, respectively. Consequently, in-situ incubation results showed two times higher than laboratory examination. We compared the material flux at the sediment-water interface of each farm and the characteristics between two different kinds of material flux examination.

케미컬 라이트에 의한 각결막 화상 1예

박정현,송낭희,지남철,고재웅,Jung Hyun Park,MD,Nang Hee Song,MD,Nam Chul Chi,MD,PhD,Jae Woong Koh,MD,PhD 대한안과학회 2010 대한안과학회지 Vol.51 No.9

Purpose: Chemical lights, also called Luminous Sticks, consist of a solution of diphenyl oxalate (C14H10O4) and hydrogen peroxide (H2O2). Human tissue can be damaged when the mixed solution contacts the human body. The authors report a single case of chemical injury of keratoconjunctiva by exposure to chemical lights. Case summary: A 47-year-old man’s right eye accidentally contacted the fluorescent material when breaking a Luminous Stick 7 days before being referred to our clinic. He had pain in the right eye and experienced visual loss. The patient’s best corrected visual acuity in the right eye was 20/50. An ulcerative lesion with edema at the inferior bulbar and palpebral conjunctiva and coneal epithelial defect was observed upon biomicroscopic examination. The patient was hospitalized and antibiotics, steroids, mydriatic and artificial tear eye drops were applied for treatment. After 9 days of treatment, the best corrected visual acuity of the patient recovered to 20/20, and the conjunctiva and cornea were mostly healed. No complication was observed. Conclusions: Chemical lights are commonly used in concerts and festivals. If the contents contact the eyes when breaking the chemical lights, various chemical burns can occur and cause ophthalmologic complications. Since no regulations have been passed regarding chemical lights, safety education and supervision are considered to be necessary for children. J Korean Ophthalmol Soc 2010;51(9):1282-1286

자연 퇴행한 양안 망막모세포종

박정현,정호경,곽상인,유영석,Jung Hyun Park,Ho Kyung Chung,Sang In Kwarg,Young Suk Yu 대한안과학회 2006 대한안과학회지 Vol.47 No.12

Purpose: To report four cases of spontaneous regression of retinoblastoma. Methods: The clinical courses of two patients were retrospectively investigated: a patient who has been diagnosed with bilateral retinoblastoma and has survived more than six years after his parents refused treatment and a patient with bilateral retinal lesions who has survived nine years whose sister was treated for unilateral retinoblastoma. Results: A nine-year-old girl, diagnosed with bilateral retinoblastoma when she was three months old, remained well for nine years after her parents refused to seek treatment. The right eye had three retinal masses that had regressed, and the left eye showed signs of phthisis and was enucleated for a cosmetic purpose. Microscopic examination of the left eye showed a mass, which consisted mostly of fibroblasts and ground substance and a few retinoblasts without mitotic figures. A six-year-old boy with a family history of retinoblastoma presented with regressed retinal masses in both of his eyes at the first funduscopic examination. The retinal lesions showed no change until his ninth year. Conclusions: Two cases of spontaneous regression of bilateral retinoblastoma were observed. No distant metastasis was detected during long-term follow-up. The retinal lesions were similar to regressed masses after treatment.

낙동강 하구역 진우도 자연해빈의 지하수위 변동해석

박정현,윤한삼,이인철,Park, Jung-Hyun,Yoon, Han-Sam,Lee, In-Cheol 해양환경안전학회 2014 해양환경안전학회지 Vol.20 No.4

This study selected five observational stations in the normal direction of Jinu-do(island) shoreline and observed water temperature, electrical conductivity and pressure from March, 2012 to January, 2013(about 11 months) and attempted to see the variation characteristics of ground watertable. This study wants to know : 1) External environment force factors(tide, climate, wave etc.) affecting ground watertable variation through time series and correlation analysis. 2) Spatial variations of ground watertable and electrical conductivity change by storm event. First, we found that the station at the intertidal zone was strongly affected by wave and tide level and the stations at sand dune and vegetation zone was affected by precipitation and tide level through time series data and correlation analysis. Second, during the storm event, we found that ground watertable and electrical conductivity are stabilized at the start line of sand dune and vegetation zone and transition zone between freshwater layer and seawater layer exists in the experiment area and is about 50~70 m from coastline of the south side of Jinu-do(island). 본 연구는 낙동강 하구역의 사주섬 중 최서측에 위치하는 진우도를 대상으로 실험구를 구축한 후 해안선의 법선 방향으로 관측점 5곳을 선정하여 2012년 3월부터 2013년 1월까지 약 11개월간 관측된 수온, 전기전도도, 압력값을 이용하여 지하수위 변동해석을 하였다. 1) 지하수위의 수온, 염분, 수위변동과 조위, 파랑, 강수량 등의 외력조건과의 시계열 분석 및 상관분석을 통하여, 소상대 정점에서는 파랑 및 조위의 영향, 식생 및 모래지반 정점에서는 강수량 및 조위의 영향을 크게 받음을 알 수 있었다. 2) 고파랑내습시 관측점간의 지하수위 및 전기전도도의 공간적 거동을 살펴본 결과 사구(Sand dune) 및 식생대(Vegetation zone)가 시작되는 지점에서 담수층과 해수층의 천이 영역(Transition zone)이 존재하였고, 진우도 남측을 기준으로 해안선에서 50~70 m 사이에 위치함을 알 수 있었다.

사람 Mesenchymal stromal cell(hMSC) 분리를 위한 간소화된 방법에 대한 연구

박정현,김경화,이용무,구영,류인철,한수부,정종평,Park, Jung-Hyun,Kim, Kyoung-Hwa,Lee, Yong-Moo,Ku, Young,Rhyu, In-Chul,Han, Soo-Boo,Chung, Chong-Pyoung 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.1

많은 연구들에서 hMSC를 얻기 위해 centrifugation, fluoroscence activated cell sorter(FACS), magnetic activated cell sorter(MACS)가 이용되어져 왔다. 그러나 centrifugation만을 이용한 경우 순도가 떨어지며 FACS나 MACS의 경우에는 비용, 시간이 많이 드는 단점이 있다. 따라서 이 연구에서는 antibody cocktail을 이용하여 hMSC를 좀더 쉽게 얻어내는 방법에 대해 알아보았다. 사람의 골반에서 12G의 바늘을 이용하여 골수를 흡입한 후 heparin이 들어있는 시험관에 넣고 처리과정을 시행하기 전에 냉장고에 보관하며 가능한 한 빨리 처리 과정을 실시한다. 얻은 골수에 적당량의 RosetteSep( Stemcell Technologies)을 첨가한 후 실온에서 20분간 반응시킨다. 그 후 적당량의 Ficoll-paque위에 골수와 RosetteSep의 혼합물을 섞이지 않게 올리고 원심분리를 이용하여 원하는 세포층을 얻어낸다. 이 세포층을 따로 분리한 뒤 배양한다. 배양 시 세포가 80%이상 차기 전에 계속 passage를 시행하며 배양한다. 이는 세포가 밀도가 높아져 원치 않는 세포로 분화되는 것을 막기 위함이다. 배양된 세포가 다양한 분화능력을 가지고 있는지 알아보기 위해 세 가지로 분화를 유도하였다. 적절한 배지와 적절한 환경에서 배양함으로써 얻어진 세포를 osteoblast, chondroblast, adipocyte로 분화를 유도하였다. 분화된 세포가 원하는 형질의 세포로 분화되었는지를 확인하기 위하여 osteoblast의 경우 alizarin red staining, alkaline phosphatase activity, chondroblast의 경우 toluidine blue staining, adipocyte의 경우 Oil-Red-O staining으로 염색하여 분화를 확인하였다. 분리해낸 세포는 각각 세 가지 세포로 분화가 되었으며 이는 RosetteSep이 hMSC를 성공적으로 분리해냈다는 것을 보여준다. 그러나 모든 세포가 분화를 보이지는 않았으며 따라서 hMSC의 순도를 높이기 위한 연구가 더 필요하다. RosetteSep을 이용하면 다른 방법들 보다 쉽게 hMSC를 얻을 수 있으나 기존의 방법과 순도의 측면에서 더 비교할 필요가 있다.

PSZ, Al₂O₃, TiO₂를 반응소결하여 제조한 쾌삭(快削) 세라믹스

박정현,정동식,이원재,김일수,Park, Jeong Hyun,Jung, Dong Sik,Lee, Won Jae,Kim, Il Soo 한국세라믹학회 2012 한국세라믹학회지 Vol.49 No.6

Machinability is important in engineering applications, especially in the current micro-electronics industry. Most ceramic components have complex shapes and hence require machining generally with diamond tools, which incurs a high production cost. Recently, h-BN-containing machinable ceramics have been developed, but these materials are very expensive due to the high raw materials and production costs. Therefore, the development of low-cost machinable ceramics is necessary. In this study, inexpensive $Al_2TiO_5$ was studied as a replacement for h-BN. $Al_2O_3$, $TiO_2$ and partially stabilized $ZrO_2$(PSZ) powders were mixed with various mole ratios and were sintered at $1500^{\circ}C$ for 1 h. The density, hardness and strength were then measured. The phase analysis and microstructures were observed by XRD and SEM, respectively. The machinability of each specimen was tested by micro-hole machining. The results of this research showed that the produced composites could be used as low-cost machinable ceramics.