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한외여과된 돈혈청으로부터 DEAE - Sephacel Chromatography 에 의한 면역단백질의 분리
전기홍,유익종,박우문,이무하 ( K . H . Jeon,I . J . Yoo,W . M . Park,M . H . Lee ) 한국축산학회 1997 한국축산학회지 Vol.39 No.1
Concentrated porcine serum with ultrafiltration 30KDa was purified with DEAF-Sephacel column chromatography. Buffer solution with pH 7 to 9 and buffer concentration with 0.01M to 0.05M, and NaCl gradient with 0M∼0.5M, 0M∼0.8M and 0M∼1.0M were used respectively for chromatographic purification. Optimal conditions were at pH 8.0, buffer conc. 0.03M and salt conc, 0M∼0.5M with immunoprotein concentration 82% and production yield 43%. Purified immunoprotein was identified in the molecular weight 50KDa and 15KDa by electrophoresis.
발효식품에서 분리한 젖산균이 발효소시지의 저장중 이화학적 특성에 미치는 영향
박우문,최원희,유익종,김왕준,지중룡,정동효 ( W . M . Park,W . H . Choi,I . J . Yoo,W . J . Kim,J . R . Ji,D . H . Chung ) 한국축산학회 1997 한국축산학회지 Vol.39 No.1
The effects of Lactobacillus plantarum, which produce bacteriocin, isolated from fermented foods (NFS #6-6, fermented sausage: S #3, kajami sikhae) on the physico-chemical properties of fermented sausages were compared with commercial starter culture, SL during storage at 24℃ and 35℃. Sausages treated with NFS #6-6 and S #3 had higher a value than SL at 24℃. Saturated fatty acid (SFA) were increased but unsaturated fatty acid (USFA) were decreased after 4 weeks storage and that ratio were high as the storage temperature were increased, regardless of treated starters. Free amino acid contents were decreased but glutamic acid, lysine and aspartic acid were detected in large amount as compared with other free amino acids after 4 weeks storage, regardless of storage temperatures and treated starters. TBA values were 0.24∼0.27 ㎎/㎏ at 24℃ and 0.49∼0.52 ㎎/㎏ at 35℃ after 4 weeks storage and did not show any difference among samples.
4M 및 HAZOP 기법을 활용한 연구실 위험요소 분석 연구
김태훈 ( T H Kim ),이광원 ( K W Rhie ),서두현 ( D H Seo ),이익모 ( I M Lee ),윤충식 ( C S Yoon ),이윤근 ( Y K Lee ),박정임 ( J I Park ) 한국안전학회(구 한국산업안전학회) 2013 한국안전학회지 Vol.28 No.3
In university laboratories, areas of studies are becoming diverse and complicated according to the development of the industry. New forms of potential risk factors are increasing and they are unlike existing ones. In addition, many students are conducting various experiments in the laboratory. Therefore, they could be exposed to risk more often. Despite these risks, people do not recognize university lab safety activities properly and observe safety precautions. They are exposed to various laboratory accidents continually. In this study, we do not apply the present diagnosis method, checklist, but the safety assessment that is widely used in industry. Then we can find lots of hazard that checklist method could miss. This study will use the 4M and Hazard & Operability to design a new Laboratory safety assessments method.
전자식 커플링 Wheel Shaft의 랙 전조 유한요소해석
박세제(S. J. Park),김동환(D. H. Kim),김영환(Y. H. Kim),박인돈(I. D. Park),김병민(B. M. Kim) 한국소성가공학회 2012 한국소성가공학회 학술대회 논문집 Vol.2012 No.10
This paper research interpretive method about thread rolling for wheel shaft. Firstly, the analysis is focused on the effective analysis conditions that guarantee the reliability of the analysis time. The investigated parameters are the shape of modeling and the analysis method. Using the results, capable of reducing for analysis time to optimum of form rolling that guarantee the results are found. This paper presented thread rolling process analysis of method that has not been much research on the domestic.