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효모 유전자 발현용 (發現用) Promoter 개발에 관한 연구
정동효,정호권,박준희,심상국 ( Dong Hyo Chung,Ho Kwon Chung,Joon Hee Park,Sang Kook Shim ) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
The purpose of this study was the development of promoter for the lacZ` gene. Two heterologous promoter Ⅰ and Ⅱ of lacZ` gene were isolated from chromosomal DNA Bam HI fragment of yeast. The size of the promoter Ⅰ was estimated to be 2.5 kb and β-galacrosidase activity was 124.6 U/㎎ protein, and the size of the promoter Ⅱ was 4.0 kb and its β-galactosidase activity was 168.8 U/㎎ protein, respectively. The stability of the recombinant YEp plasmid in the transformant was from 52.7 to 67.4% at minimal medium. YIp plasmid was constructed from YEp plasmid, and expressed both in E. coli and yeast. The promoter Ⅰ aid Ⅱ iso-laced from yeast chromosomal DNA can be used for promoter of plasmid YEp and YIp.
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송유섭,신상섭,정동호,박대효,Song, Yoo-Seob,Shin, Sang-Shup,Jung, Dong-Ho,Park, Tae-Hyo 한국전산구조공학회 2011 한국전산구조공학회논문집 Vol.24 No.6
In this paper, the behavior of nuclear-power plant subjected to an aircraft impact is performed using the parallel analysis. In the erstwhile study of an aircraft impact to the nuclear-power plant, it has been used that the impact load is applied at the local area by using the impact load-time history function of Riera, and the target structures have been restricted to the simple RC(Reinforced Concrete) walls or RC buildings. However, in this paper, the analysis of an aircraft impact is performed by using a real aircraft model similar to the Boeing 767 and a fictitious nuclear-power plant similar to the real structure, and an aircraft model is verified by comparing the generated history of the aircraft crash against the rigid target with another history by using the Riera's function which is allowable in the impact evaluation guide, NEI07-13(2009). Also, in general, it is required too much time for the hypervelocity impact analysis due to the contact problems between two or more adjacent physical bodies and the high nonlinearity causing dynamic large deformation, so there is a limitation with a single CPU alone to deal with these problems effectively. Therefore, in this paper, Message-Passing MIMD type of parallel analysis is performed by using self-constructed Linux-Cluster system to improve the computational efficiency, and in order to evaluate the parallel performance, the four cases of analysis, i.e. plain concrete, reinforced concrete, reinforced concrete with bonded containment liner plate, steel-plate concrete structure, are performed and discussed.
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김성범,이경재,정동호,박대효,Kim, Seongbeom,Lee, Kyungjae,Jung, Dongho,Park, Taehyo 대한토목학회 2014 대한토목학회논문집 Vol.34 No.1
In recent years, the structural shock response to UNDEX (UNDerwater EXplosion) has been studied as much, or more, through numerical simulations than through testing for several reasons. Very high costs and sensitive environmental concerns have kept destructive underwater explosion testing to a minimum. Increase of simulation capabilities and sophisticated simulation tools has made numerical simulations more efficient analysis methods as well as more reliable testing aids. In this study, the main issue is the fluid-structure interaction. Here, appropriate relations between the acoustic pressure on the fluid surface and displacements on the structure surface are formed internally. The analysis was carried out using ABAQUS/Explicit and the results have been visualized in ABAQUS CAE. The shock loading history, acoustic pressure, stress of stand-off point, the velocity and strain energy time histories were presented.
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