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한외여과된 돈혈청으로부터 DEAE - Sephacel Chromatography 에 의한 면역단백질의 분리
전기홍,유익종,박우문,이무하 ( K . H . Jeon,I . J . Yoo,W . M . Park,M . H . Lee ) 한국축산학회 1997 한국축산학회지 Vol.39 No.1
Concentrated porcine serum with ultrafiltration 30KDa was purified with DEAF-Sephacel column chromatography. Buffer solution with pH 7 to 9 and buffer concentration with 0.01M to 0.05M, and NaCl gradient with 0M∼0.5M, 0M∼0.8M and 0M∼1.0M were used respectively for chromatographic purification. Optimal conditions were at pH 8.0, buffer conc. 0.03M and salt conc, 0M∼0.5M with immunoprotein concentration 82% and production yield 43%. Purified immunoprotein was identified in the molecular weight 50KDa and 15KDa by electrophoresis.
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반응표면 분석법을 이용한 계란 난황 레시틴 분리 최적조건
장애라,임동균,전희준,조철훈,김일준,이무하,Jang, Ae-Ra,Lim, Dong-Gyun,Jeon, Hee-Joon,Jo, Cheo-Run,Kim, Il-Joon,Lee, Moo-Ha 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.4
The optimum conditions for the extraction of lecithin from egg yolk were determined using response surface methodology (RSM). On the basis of the results of preliminary experiments, the most effective values were selected. The effects of three independent variables, dilution ratio, solvent composition, and extraction temperature on the response of crude egg lecithin (g) were then determined. The optimum conditions for egg lecithin separation obtained using ridge analysis were 6.51, 95.83%, and $40.2^{\circ}C$ for the dilution ratio, solvent composition, and extraction temperature, respectively. Using the optimum conditions, 55.04% of crude lecithin in total phospholipid can be obtained from 100 g liquid egg yolk. The experimental values (56.21% crude lecithin in total phospholipid) agreed with the predicted values.
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