감염 증상 없이 발견된 Balantidium coli 1예

문연숙,김한성,남정현,최종원 대한진단검사의학회 2004 Annals of Laboratory Medicine Vol.24 No.4

Balantidium coli is widely distributed in hogs, particularly in warm and temperate climates, and in monkeys in the tropics. B. coli is the only pathogenic ciliate and is the largest protozoan parasitizing humans. Some individuals with B. coli infections are totally asymptomatic, whereas others have symptoms of severe dysentery similar to those seen in patients with amebiasis. We report a 5-year-old girl with asymptomatic balantidiasis. The patient was suffering from herpes zoster for several days. She did not have symptoms of dysentery or urinary tract infection. Motile trophozoites of B. coli were observed in the urinary sediment. This is the first report of asymptomatic balantidiasis in Korea. (Korean J Lab Med 2004; 24: 234-6) Balantidium coli는 열대 및 온대 지방의 돼지와 열대 지방의 원숭이에서 흔히 발견되는 원충으로, 인체 감염을 일으키는 유일한섬모충이며, 인체에서 발견되는 원충 중에 크기가 가장 크다. 감염된 경우 아메바성 이질과 유사한 증상을 보일 수 있으나, 무증상으로 지나는 경우도 흔하다. 무증상의 5세 여자 환자에서 B. coli가 발견되었다. 환자는 대상포진에 이환되어 있었고, 대장 감염이나 비뇨기 감염 증상이 없었다. 요침사 검경에서 빠른 운동성을 보이는 영양형이 관찰되었고, 농뇨나 혈뇨 소견은 없었다. 저자들은국내에서 처음으로 무증상 환자에서B. coli를 발견하였기에 이를보고한다.

화장품 소재로서 홍삼식이뇨(尿)의 세포 생리 활성 효능연구

문연숙,강상모 한국미용학회 2019 한국미용학회지 Vol.25 No.4

In this study, the cell experiments were carried out using heated urine (obtained through heat treatment of normal urine to remove microbes) and heated urine containing red ginseng (obtained through heat treatment of urine after taking red ginseng extract to increase functionality) in order to use urine as cosmetic material. RAW 264.7 macrophage, B16F10 melanoma, and CCD-986sk fibroblast were used to measure cytotoxicity of each urine sample, and toxicity for skin cells in each urine sample was hardly found. From the result of having measured the inhibitory rate of ROS production in the cells through DCF-DA, it was observed that the inhibitory effect of ROS production in each urine sample was the highest in heated urine containing red ginseng, followed by heated urine and then normal urine. From the result of having measured the anti-inflammatory effect in each urine sample using RAW 264.7 macrophage cell, it was found that the inhibitory effect of NO production (which inhibits the melanin biosynthesis in the B16F10 melanoma cells of each urine sample) was the highest in heated urine containing red ginseng, followed by heated urine and then normal urine. From the result of having measured the level of MMP-1 of CCD-986sk fibroblast cells in each urine sample, it was observed that heated urine containing red ginseng had a significant decrease (61,25%(p<0.01)) in the expression level of MMP-1 at a concentration of 5 μg/mL in comparison to those of normal urine or heated urine. Moreover, the synthesis of MMP-1 concentrationdependently tended to decrease in each urine sample, and the expression level of MMP-1 was most decreased in heated urine containing red ginseng, followed by normal urine and heated urine. Therefore, heated urine containing red ginseng indicates it may have a positive effect on skin improvement and anti-aging. Such functionality enables to play an important role in the development of natural cosmetics.

급성 골수성 백혈병과 비정형 급성 림프구성 백혈병의 혼합 백혈병 : 2 예 보고 a report of 2 cases

문연숙,김용구,한경자,심상인 대한임상병리학회 1996 대한임상병리학회지 Vol.16 No.6

장기 및 혈액공여자를 위한 선별검사의 분석

문연숙,강지민,김용구,한경자,심상인 대한임상검사정도관리학회 1994 臨床檢査와 精度管理 Vol.16 No.2

재생 불량성 빈혈 환자에서 발생한 원발성 골수 림프종 1예

문연숙,이안희,김병기,엄현석,한치화 大韓血液學會 2000 大韓血液學會誌 Vol.35 No.2

Klebsiella Pneumoniae Associated Extreme Plasmacytosis

문연숙,장우리,이현규,박인서,남정현,최종원,김진주,한승백 대한감염학회 2013 Infection and Chemotherapy Vol.45 No.4

Infection-associated plasmacytosis is not uncommon; however, marked plasmacytosis in both peripheral blood and bone marrowthat mimicks plasma cell leukemia is a very rare condition. We encountered a case of extreme plasmacytosis associatedwith Klebsiella pneumoniae sepsis in an aplastic anemia patient. A 42-year-old man presented with high fever of 5 days’ duration. Hematological analysis revealed severe neutropenia and thrombocytopenia; his white blood cell count was 900/mm3,with 26% of plasma and plasmacytoid cells in peripheral blood. Bone marrow biopsy and aspiration showed 25% cellularitywith marked plasmacytosis (80%), highly suggestive of plasma cell leukemia. On the eighth hospital day, K. pneumoniae wasidentified in blood and sputum cultures. Fever improved after switching antibiotics, although his hematological condition worsened. His bone marrow cellularity (plasma cell proportion) progressively decreased: the values were 25% (80%), 10% (26%),10% (11%), and < 10% (< 4%) on the 8th, 30th, 60th, and 90th hospital day, respectively. His plasmacytosis was extremelysevere but was confirmed to be reactive with polyclonality. The present case represents the first report of strong suspicion of K. pneumoniae sepsis-associated marked plasmacytosis in an aplastic anemia patient.

화장품 소재로서 홍삼식이뇨(尿)의 항산화 활성

문연숙,이민혁,강상모 한국미용학회 2019 한국미용학회지 Vol.25 No.2

In this study, we looked into the antioxidant effect of heated urine containing red ginseng, especially which red ginseng is used as raw material for cosmetics. Experimental materials were classified into three types: normal urine, heated urine, and heated urine containing red ginseng. Heated urine containing red ginseng refers to a urine that contains 9.54 ng/mL of compound K after red ginseng ingestion. Each urine sample was acquired from the following method: repeated 30-munite pasteurization of classified urines in a tank (80oC) 3 times every 15 minutes, concentrated and freeze-dried them. For measurement of antioxidation, DPPH, ABTS, and FRAP as well as total content of Polyphenol and Flavonoids were measured. As to the total content of Polyphenol for each urine sample, 84.33 ± 2.17 mg/g was for normal urine, 85.16 ± 4.22 mg/g for heated urine, and 112.24 ± 4.65 mg/g for heated urine containing red ginseng. And as to the total content of Flavonoids, 5.20 ± 0.21 mg/g was for normal urine, 5.58 ± 0.39 mg/g for heated urine, and 15.90 ± 0.83 mg/g for heated urine containing red ginseng. Therefore, the total content of above two ingredients appeared higher in order of heated urine containing red ginseng, heated urine and normal urine. As to DPPH, at a concentration of 10 mg/mL, scavenging activity was observed in normal urine (36.79%), heated urine (41.54%), and heated urine containing red ginseng (54.62%) respectively. As to ABTS, at a concentration of 10 mg/mL, scavenging activity was observed in normal urine (50.51%), heated urine (55.64%), and heated urine containing red ginseng (73.79%) respectively. As to FRAP, iron-ion reducing power was found in normal urine (0.065 ± 0.004 mmol AAE/g), heated urine (0.066 ± 0.004 mmol AAE/g), and heated urine containing red ginseng (0.168 ± 0.006 mmol AAE/g) respectively. Therefore, the study result showed that heated urine containing red ginseng would be highly likely to be an antioxidant ingredient.

The 2016 WHO versus 2008 WHO Criteria for the Diagnosis of Chronic Myelomonocytic Leukemia

문연숙,김미향,김혜련,안정열,허정원,허지영,한재호,박준성,조성란 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.5

The 2016 WHO diagnostic criteria for chronic myelomonocytic leukemia (CMML) require both absolute and relative monocytosis (≥1×109/L and ≥10% of white blood cell counts) in peripheral blood. Moreover, myeloproliferative neoplasm (MPN) features in bone marrow and/or MPN-associated mutations tend to support MPN with monocytosis rather than CMML. We assessed the impact of the 2016 WHO criteria on CMML diagnosis, compared with the 2008 WHO criteria, through a retrospective review of the medical records of 38 CMML patients diagnosed according to the 2008 WHO classification. Application of the 2016 WHO criteria resulted in the exclusion of three (8%) patients who did not fulfill the relative monocytosis criterion and eight (21%) patients with an MPN-associated mutation. These 11 patients formed the 2016 WHO others group; the remaining 27 formed the 2016 WHO CMML group. The significant difference in the platelet count and monocyte percentage between the two groups indicated that the 2016 WHO criteria lead to a more homogenous and improved definition of CMML compared with the 2008 WHO criteria, which may have led to over-diagnosis of CMML. More widespread use of molecular tests and more sophisticated clinical and morphological evaluations are necessary to diagnose CMML accurately.

A Novel Translocation Involving RUNX1 and HOXA Gene Clusters in a Case of Acute Myeloid Leukemia with t(7;21)(p15;q22)

문연숙,Douglas E. Horsman,R. Keith Humphries,박경신 대한면역학회 2013 Immune Network Vol.13 No.5

Translocations involving chromosome 21q22 are frequently observed in hematologic malignancies including acute myeloid leukemia (AML), most of which have been known to be involved in malignant transformation through transcriptional dysregulation of Runt-related transcription factor 1 (RUNX1) target genes. Nineteen RUNX1 translocational partner genes, at least, have been identified, but not Homeobox A (HOXA) genes so far. We report a novel translocation of RUNX1 into the HOXA gene cluster in a 57-year-old female AML patient who had been diagnosed with myelofibrosis 39 months ahead. G-banding showed 46,XX,t(7;21)(p15;q22). The involvement of RUNX1 and HOXA genes was confirmed by fluorescence in situ hybridization.

혈액질환에서 혈소판 지수들의 임상적 의의

이제훈,문연숙,김용구,한경자,심상인 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.2