A cell-penetrating peptide blocks Toll-like receptor-mediated downstream signaling and ameliorates autoimmune and inflammatory diseases in mice

권혁권,Mahesh Chandra Patra,신현준,Xiangai Gui,asma,Suresh Panneerselvam,김동진,송석종,홍리원,김경수,김양균,Francis Y. Lee,함대현,이상호,최상둔 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Toll-like receptors (TLRs) recognize pathogen/damage-associated molecular patterns and initiate inflammatory signaling cascades. Occasionally, overexpression of TLRs leads to the onset of numerous inflammatory diseases, necessitating the development of selective inhibitors to allow a protective yet balanced immune response. Here, we demonstrate that a novel peptide (TIP1) derived from Toll/interleukin-1 receptor (TIR) domain-containing adapter protein inhibited multiple TLR signaling pathways (MyD88-dependent and MyD88-independent) in murine and human cell lines. TIP1 also inhibited NLRP3-mediated IL-1β secretion, as we validated at both the protein and mRNA levels. Biophysical experiments confirmed that TIP1 specifically binds to the BB loop of the TLR4-TIR domain. Animal studies revealed that TIP1 inhibited the secretion of lipopolysaccharide (LPS)-induced proinflammatory cytokines in collagen-induced arthritis (CIA) and kaolin/carrageenan-induced arthritis (K/C) rodent models. TIP1 also rescued animals from sepsis and from LPS-induced kidney/liver damage. Importantly, TIP1 ameliorated the symptoms of rheumatoid arthritis in CIA and K/C rodent models, suggesting that TIP1 has therapeutic potential for the treatment of TLR-mediated autoimmune/inflammatory diseases.

완전좌각차단(Complete LBBB)환자의 균형마취

임유택,권혁권,정병연,김세강,정용일 대한마취과학회 1996 Korean Journal of Anesthesiology Vol.31 No.6

Local or general anesthesia is essential for safe operation. Patients in good preoperative condition are relatively to tolerable to the operation, but the patients in poor preoperative condition have the high mortality and morbidity during and after the operation. Therefore, we should choose the anesthetic agents and methods, which cause little effect to the patients life. Among the intraventricular blocks, bundle branch is the most common type, and left bundle branch block may progress to a more serious condition of complete heart block. Optimal anesthetic management of patients with cardiovascular disease requires a thorough knowledge of normal cardiac physiology, the circulatory effects of the various anesthetic agents, and the pathophysiology and treatment of these diseases. The authors successfully performed balanced anesthesia in operation of a 63 year old female patient who showed complete left bundle branch block pattern in a preoperative electrocardiographic tracing without any subjective symptoms.

갈색세포종 적출수술시의 Isoflurane 마취

임유택,권혁권,김세광,정용일,정병연 대한마취과학회 1997 Korean Journal of Anesthesiology Vol.32 No.1

We have experienced an anesthetic management of a 34 year old female patient with pheochromocytoma of left adrenal gland. The anesthetic management of patients presents many difficult problems, such as hypertension, arrhythmia and hypotension. The patient had been treated with phenoxybenzamine for 2 weeks preoperatively. Following induction of anesthesia with intravenous fentanyl, thiopental sodium and vecuronium, endotracheal intubation was performed. Anesthesia was maintained with nitrous oxide, oxygen and isoflurane administration. Blood pressure and pulse were controlled well with nitroprusside and isoflurane. After removal of tumor, blood pressure was controlled by Hartman's solution, packed red cell and dopamine administration. The patient tolerated well despite the episodic hemodynamic changes. Importance of preoperative preparation, sufficient sedation, smooth induction, complete analgesia, good muscle relaxation and stable cardiovascular control has been discussed. (Korean J Anesthesiol 1997; 32: 122∼126)

Prazosin 수술전 처치후 갈색세포종 마취관리에 대한 임상적고찰

조옥현,권혁권,구정서 대한마취과학회 1991 Korean Journal of Anesthesiology Vol.24 No.1

Perioperative management of patients with pheochromocytoma is challenging. Accordingly, proper preoperative preparation is important. Prazosin, a selective alpha I blocker, may offer a potential advantage. This 54-year-old woman was treated with prazosin 2 mg, b.i.d. for 15 days and also with propranolol 20 mg, b.i.d. for a few days intermittently before the proposed surgery. Both symptoms and blood pressure were well controlled effectively. Induction of anesthesia was accomplished with nitrolingual spray, fentanyl 100 μg, 1% idocaine 50 mg, 2.5% thiopental sodium 200 mg, vecuronium 6 mg and 100% O₂-enflurane. During the surgical and anesthetic procedure, the patient showed a reduced incidence of excessive blood-pressure variations and no arrhythmia was present except for supraventricular ectopic beats.

개량 습윤제의 Streptococcus mutans 및 Candida albicans에 대한 영향

박영민,권혁권,여규영,이은숙,한만덕,정인식 ( Young Min Park,Hyuk Kon Kwon,Kue Young Yeo,Eun Sook Lee,Man Deuk Han,In Sik Chung ) 대한예방치과·구강보건학회 1997 大韓口腔保健學會誌 Vol.21 No.2

제왕절개술후 경막외강과 정맥내 PCA를 이용한 진통효과와 만족도의 비교

정용일,정병연,권혁권 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.36 No.2

Background : Epidural administration of local anesthetics and opiate or intravenous administration of opiate and ketorolac has proven to be effective in the treatment of postoperative pain. Studies that compare epidual morphine-bupivacaine vs intravenous nalbuphine-ketorolac administration showed conflicting results. We compared the ability and side effects of epidural (EPI-PCA) morphine-bupivacaine versus intravenous (IV-PCA) nalbuphine-ketorolac for postoperative pain relief after cesarean delivery. Method : Sixty healthy women were randomly assigned to receive an epidural bolus of morphine 3 mg mixed with 0.5% bupivacaine 10 ml, followed by a EPI-PCA with 0.0125% morphine and 0.125% bupivacaine (basal infusion 2 ml/hr, bolus 0.5 ml, lock-out interval 15 min) or intravenous bolus of nalbuphine 5 mg, followed by a IV-PCA with 0.05% nalbuphine and 0.15% ketorolac (basal infusion 2 ml/hr, bolus 0.5 ml, lock-out interval 30 min) for pain relief after cesarean delivery. The intensity of pain was assessed by the patient, who was unawared of the dose given, using a visual analog scale (VAS). To compare intensity of pain, VAS was used at 1, 6, 12, 24 and 40 hour after the end of surgery. Result : EPI-PCA group had significant lower visual analog scale (VAS) at immediate postoperative period, whereas no significant difference was observed when pain was assessed at other time sequence. Pruritus was more frequent with EPI-PCA group, although the incidence of other side effects were the same. Conclusion : We conclude that EPI-PCA or IV-PCA using morphine-bupivacaine or nalbuphine- ketorolac is relatively effective and safe method for the postoperative pain control. Although EPI-PCA with morphine-bupivacaine shows lower VAS at immediate postoperative period, IV-PCA with nalbuphine-ketorolac is a safe and effective alternative to EPI-PCA with morphine-bupivacaine for providing pain relief after cesarean delivery. (Korean J Anesthesiol 1999; 36: 268∼272)

미추마취하의 수술시 Morphine과 혼합한 Mepivacaine, Bupivacaine의 투여에 따른 마취 발현시간 및 술후 제통효과의 비교

정용일,임유택,정병연,권혁권 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.36 No.2

Background : Caudal injection of local anesthetics with morphine is the most common anesthetic technique for perianal operation and postoperative analgesia. This study was purposed to compare the onset time of caudal analgesia, postoperative analgesic effect and side effects. Method : Sixty healthy patients scheduled for perianal operation were divided into 2 groups randomly. Group I was given 2 mg of morphine in 20 ml of 2% mepivacaine via sacral hiatus. Group II was also given 2 mg of morphine in 20 ml of 0.5% bupivacaine caudally. We measured the onset time of caudal block, time to the first request of analgesics, the number of analgesics within 24 hours and the incidence of postoperative side effects. Analgesic effect was evaluated by visual analogue scales (VAS) at 1, 2, 6, 12 and 24 hours postoperatively. Result : The onset time of caudal block for operation and the first request time of analgesic for postoperative pain was significantly shorter in group I than group II. The analgesic use in the first 24 hours was significantly more in group I than group II. The side effects were similar in both groups. Conclusion : We concluded that the combined use of morphine and bupivacaine provided better postoperative analgesia than the combined use of morphine and mepivacaine. (Korean J Anesthesiol 1999; 36: 263∼267)

고순도 Galactooligosaccharide 제조 및 유산균 증식 활성

홍기배,서형주,김재환,권혁권,박청,한성희,Hong, Ki Bae,Suh, Hyung Joo,Kim, Jae Hwan,Kwon, Hyuk Kon,Park, Chung,Han, Sung Hee 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.6

본 연구는 ${\beta}$-galactosidase와 효모 발효에 의해 고순도 갈락토올리도당(HP-GOS)의 제조를 위한 효율적인 방법을 찾고자 수행하였다. 6종의 상업용 ${\beta}$-galactosidase를 이용해 효소반응 전후의 구성당을 각각 비교한 결과, Lactozym 3000L을 이용하여 제조한 GOS에서 효소반응을 통해 생성된 total GOS의 양이 가장 높은 것으로 나타났다. 생성된 GOS의 농도는 초기 lactose로부터 51%의 전환율을 나타냈다. 또한 GOS의 수율은 효모(Saccharomyces cerevisiae)의 발효에 의해 더욱 높아졌는데, 효모를 8% 첨가하여 36시간 발효 후 생성된 GOS의 농도는 71%까지 도달했음을 확인하였다. HPLC를 이용한 구성당 분석 결과, S. cerevisiae에 의한 발효를 통해 제조한 HP-GOS에는 발효 전에 존재했던 glucose의 함량이 급감되었을 뿐 아니라, 4'/6'-galactosyllactose와 total GOS의 양은 유의적으로 증가되었다. HP-GOS는 상업용 GOS보다 Lactobacillus 속(L. acidophilus and L. casei) 및 Bifidobacterium 속(B. longum and B. bifidum)의 성장을 촉진시키는 것으로 확인되었다. 이러한 결과를 종합해 볼 때, 효소 처리 및 효모 발효를 통해 고순도의 GOS가 제조되었으며, 제조된 HP-GOS는 상업용 GOS에 비해 장내 유용균으로 알려진 Bifidobacterium 속과 Lactobacillus 속의 생육을 증가시킴으로써 인간의 장내 건강에도 유익한 영향을 미칠 것으로 사료된다. This study attempted to find an efficient method for the preparation of high-purity galactooligosaccharides (HP-GOS) using ${\beta}$-galactosidase and yeast fermentation. GOS prepared using Lactozym 3000L showed the greatest enhancement in total GOS of the six ${\beta}$-galatosidases tested. GOS alone achieved 51% conversion of initial lactose. GOS production was enhanced by fermentation with commercial yeast (Saccharomyces cerevisiae); its concentration reached 71% after 36h fermentation with 8% yeast. Component sugar analysis with HPLC indicated that HP-GOS fermented with S. cerevisiae showed significantly increased levels of 4'/6'-galactosyllactose and total GOS as well as a significantly decreased glucose level. HP-GOS facilitated the growth of Lactobacillus sp. (L. acidophilus and L. casei) and Bifidobacterium sp. (B. longum and B. bifidum). In sum, high-purity GOS has been successfully produced through both an enzymatic process and yeast fermentation. GOS encourages the growth of bacteria such as Lactobacillus and Bifidobacterium that may be beneficial to human gastrointestinal health.

편도절제술을 받는 어린이에서 술전 Bupivacaine 침윤과 Fentanyl 투여가 술후 통증에 미치는 효과

정지영,정용일,정병연,권혁권,임유택,하동춘 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.33 No.6

Background : Early postoperative pain following tonsillectomy is a significant discomfort and lead to inappropriate coughing reflex and restlessness on children. We evaluated the effect of intravenous fentanyl and peritonsillar bupivacaine infiltration on postoperative pain in children. Methods : We studied the 45 children for tonsillectomy. Group I received intravenous fentanyl(1 ㎍/kg) before surgical incision. Group II received 0.5% bupivacaine(2-10 ml) with epinephrine(1:100,000) on the peritosillar area before peritonsillar incision. Group III received saline infiltration instead of bupivacaine. Results : Visual analogue scale(VAS) observed at recovery room, 1hr, 2hr, 4hr after tonsillectomy were significantly lower in group I and II than group III but VAS at 8hr were similar among 3 group. Time to first request of analgesics showed no statistical significance between fentanyl group and bupivacaine group but saline group showed statistical significance. Total required dosage of analgesics showed no statstical significance among the 3 group. Conclusions : Preincision intravenous fentanyl and bupivacaine infiltration are both effective on postoperative pain control after tonsillectomy in children. (Korean J Anesthesiol 1997; 33: 1148∼1153)

심상성 루프스 소견을 보인 BCGitis 1례

이윤정,안혜경,박천욱,이철헌,원임수,박창민,권혁권 한림대학교의료원 1993 人間科學 Vol.17 No.1