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강재승,장우현 대한미생물학회 1987 大韓微生物學會誌 Vol.22 No.1
To detect lepotospiral strains which produce hemolysin and determine the optimal condition for assaying hemolysin, we screened reference strains and observed some property of hemolysin. Hemolysin activity was assayed with cell free culture liquids and erythrocyte suspension. The production of hemolysin by local strains isolated in Korea was assayed and compared with that of reference strains. The hemolysin was produced by 18 strains among 38 reference strains and 3 local strains isolated in Korea. The production of hemolysin began with growth of Leptospira cultured in EMJH medium and reached maximum at stationary phase. The optimum temperature for hemolytic activity was 37℃. At 1ower temperature the activity of hemolysin was decreased progressively. The hemolytic activity was completely inactivated after 30 minutes exposure at 56C. Hemolysis pattern was hot-cold type which showed increased hemolysis after cold incubation. The hemolysin was most active on sheep erythrocyte and less active on ox, goat, human and guinea pig erythrocyte with the decreasing order.
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장티푸스환자의 혈청내 Salmonella typhi에 대한 IgG subclass항체의 분포
강재승,장우현,김영중,조민기,황응수,민창홍,김윤원,차창룡 대한미생물학회 1986 大韓微生物學會誌 Vol.21 No.4
To diagnose the typhoid fever rapidly and accurately in clinically suspected patients, the levels of IgG subclass antibody were measurect by enzyme-linked immunosorbent assay(ELISA). With symptom, blood culture and agglutination test, tested persons were categorized into 6 groups as typhoid fever, FUO, paratyphi A or B, other bacterial infections, cancers, and control. ELISA was performed on the polyvinyl chloride plates coated with killed whole cell (10 cell/ml) of S. typhi 0901W by poly-L-lysine applied as binding substance (and polyvinyl chloride as solid phase). We distribution of the level of 1gG subclass antibodies in each group was analyzed and compared with other groups. The results obtained were summarized as follow: 1. The optimal dilution of the sera from patients with typhoid fever was 1:160, and those of the sheep anti-human IgG subclass and the peroxidase conjugated rabbit anti-sheep IgG were 1:4000 and 1:5000, respectively. 2. The absorbance levels of IgG subclass in the sera of typhoid fever patients were as follows; a) IgG1 value is 0.439±0.110 b) IgG2 value is 0316±0.165 c) Ig43 value is 0.449±0.145 d) IgG4 value is 0.525±0.154 IgG subclass levels in the sera of typhoid patients were much higher than in control group and patient with paratyphi A or B as well as other infectious diseases. The sensitivity and the specificity in differential diagnosis of typhoid fever and other febrile diseases were 92% and 79% assay of IgG1 respectively, whereas those in the assay of IgG2 were 97% and 72%, respectively (above absorbance 0.3). 3. The absorbance levels of IgG subclass in the serial sera of typhiod fever patients tend to decrease to the level of absorbance 0.3 in 10 months from the onset of illness. 4. The order of absobance levels of IgG subclass in the serum of each group were typhoid fever, paratyphi A or B, other infectious diseases, control and cancer. 5. For the serodiagnosis of typhoid fever against other febrile diseases, the sensitivity and the specificity in the assay of IgG2 activity were 76% and 93% in absorbance 0.4, respectively, 6. In the distribution of the level of each IgG subclass in the sera of FUO patients which were suspected of typhoid fever, the positive rate was ranged from 36% to 82%. This suggest that more than 50% of FUO patients are caused by S. typhi.
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폴리페놀의 비타민 C 안정성 유지와 피부 침투성 증가에 관한 연구
강재승,조대호,이왕재 대한면역학회 2004 Immune Network Vol.4 No.4
Background: It is necessary for human beings to uptake vitamin C through diet or supplements. It is also well-known that vitamin C plays an important role in the prevention of scurvy, enhancement of collagen synthesis and anti-tumor immune response. In addition, there are several recent reports regarding the effective role of vitamin C on the regulation of allergic responses, such as atopic dermatitis and asthma. However, the effective therapeutic and preventive measures using vitamin C are not established yet, since vitamin C is seriously unstable in aqueous solution. Therefore, we have investigated the best way to maintain the stability of vitamin C. Methods: After we making a mixture of polyphenol (0.001, 0.01, 0.1%) and vitamin C (1 mM), the mixtures were placed at room temperature both with/without light protection. And then the concentration of ascorbic acid was measured with HPLC. To analyze the in vivo effect of vitamin C on the regulation of skin allergic reaction, polyphenol (0.1%)-vitamin C (1 mM) mixture was applied to the skin and the production of histamine from mast cell was analyzed by Evans blue dye staining. Results: We have found that the polyphenol has preventive power of oxidation of vitamin C. In addition, the production of histamine was suppressed by the polyphenol (0.1%)-vitamin C (1 mM) mixture. Conclusion: We have reached the conclusion that our study suggests the research guideline for the therapy of atopic dermatitis through vitamin C. (Immune Network 2004;4(4): 250-254)
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새로운 종양향원인 CM1을 이용한 종양 진단 ELISA kit의 개발
강재승,김대진,김영인,이왕재,장가용 대한면역학회 2005 Immune Network Vol.5 No.2
Background: CM1 (centrocyte/-blast marker 1) is originally defined as a germinal center B cell marker. It is known that CM1 plays a critical role on B cell development in germinal center. In addition, we have found that CM1 is expressed on lymphoma cell lines, such as Raji, Ramos and IM-9. This means that CM1 might be served as a tumor marker as well. In the present study, we examined the expression of CM1 on the surface of the other tumors and the possibility of the development of tumor screening ELISA kit by using CM1. Methods: First, we have examined the expression of CM1 on stomach cancer and hepatoma, which are predominantly (discovered) occurred in Korean, by flow cytometry analysis. After purifying of CM1 antigen from Raji and Ramos, the optimal ELISA condition was determined. And then we compared the level of CM1 between normal individuals and cancer patients by ELISA. To decrease the non-specific binding of anti-CM1 mAb with serum components except CM1 and to enhance the diagnostic accuracy, albumin depletion spin column was used. Results: CM1 was highly expressed on stomach cancer and hepatoma cell lines. In addition, we have also confirmed the increased CM1 expression on cancer patients. The difference of CM1 expression between normal individuals and cancer patients were more clearly observed, after deletion of serum albumin by using albumin depletion spin column. Conclusion: Based on the results from this study, CM1 might be a useful molecule for the early diagnosis of cancer. In addition, further studies for the increase of ELISA sensitivity and appropriate albumin depletion methods should be needed.
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MRL-lpr/lpr 마우스로부터 항-DNA 항체생성 하이브리도마세포의 합성과 항-DNA 항체의 특성 분석
강재승,김형일,김영태,윤정구 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1
The pathogenesis of systemic lupus erythematosus (SLE) an autoimmune disease, is unknown, but it is certain that anti-DNA antibodies are closely related to pathogenesis. The character and combination of the isotype, antigen specificity and charge are thought to be main factors in pathogenesis; in effect, they determine the toxicity of anti-DNA antibodies. In this study, the spleen cells of MKL-lpr/lpr mouse were fused with P3X63Ag8.653 myeloma cells, to obtain hybridoma cells which secrete various anti-DNA antibodies. From these cells, anti-DNA antibodies were separated, purified and analysed. The findings are summarised as follows: 1) 14 hybridoma clones produced monoclonal anti-DNA antibodies. 2) 14 clones showed highly positive reaction to single-stranded DNA, and 4 clones out of the 14 clones showed cross-reaction between single-stranded DNA and double-stranded DNA. No clone reacted with left-handed Z DNA. 3) 13 clones out of the 14 clones had the isotype IgG2a, K, the remaining clone had the isotype IgM, K . None of the clones had a λ chain. 4) 6 clones showed positive reaction to poly[dA-dT]·poly[dA-dT] and poly[dA]·poly[dT], 7 clones to poly[dA-dC]·poly[dG-dT], 8 clones to poly[dG-dC] and 2 clones had a positive reaction to poly[dG-dC]·poly[dG-dC]. 5) 3 clones showed negative charge of pl3.6∼7.2, 10 clones showed positive charge of pl8.0∼9.2 and the remaining clone had neutral charge of pi 7.0∼7.4. Our findings suggest that 4 antibodies, which react with double-stranded DNA and have IgG2a isotype and cationic charge, may have pathogenic activity. This finding needs to be further tested by evaluating the effects of these antibodies when injected into normal young antoimmune disease-prone mice.
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Production of the Monoclonal Antibodies against Bartonella henselae Isolated from a Korean Patient
길세희,강재승 대한미생물학회 2012 Journal of Bacteriology and Virology Vol.42 No.1
Bartonellosis is spotlighted recently as an emerging zoonosis and Bartonella henselae is reported to be the main infectious agent. In Korea, however, few studies have been made on the epidemiology and microbiology on bartonellosis. Thus, this study was conducted to produce a new monoclonal antibody that can be used for identifying B. henselae. In order to prepare monoclonal antibodies against B. henselae, we inoculated mice with the isolated strain from Korean patient and performed cell fusion experiment. The selected hybridoma clones produced monoclonal antibodies which showed positive immunofluorescence staining of bacteria and specific protein bands in western blot analysis. In order to examine whether these antibodies could be used for the identifying and quantifying Bartonella, we performed confocal microscopy and flow cytometry using the new antibodies. These monoclonal antibodies can be used as a useful tool in further researches on the biology of Bartonella.
ScienceON
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