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한국에서 분리된 렙토스피라균의 생물학적 특성에 대한 연구(1985)
이용우,이명숙,백승복,박미연,박경석,오희복,김호훈,성원근 대한미생물학회 1986 大韓微生物學會誌 Vol.21 No.3
A microbiological study for the isolation and characterization of Leptospira interrogans was performed in an attempt to define the characteristics of Leptospirosis in Korea. The results are summerised as follows. Thirty-five cultures were isolated from 11 patients with leptospirosis and 24 wild rodents captured in Paju area. The isolation rate of Leptospira from wild rodents reached 23.1%. All 35 cultures were identified as Leptospira interrogans by their characteristic morphology and motility in dark field microscopy, pathogenecity in Guinea pig and sensitivity to S-Azaguanine. Cultures formed diffuse subsurface colonies with hazy margin and were catalase, peroxidase and ox- dase positive. The fifty percent lethal dose of isolate HM 4 in Guinea pig was 8.9X10_4 org. Mouse passage method was sucecssfully applied in maintenance of fresh isolates without any loss of their original virulency.
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