항암성 생약제로 부터 항암성분의 추출 및 항암활성 측정

이영기,신석우,염곤 단국대학교 신소재기술연구소 1993 신소재 Vol.3 No.-

암치료에 많이 사용되는 생약제 16종을 대상으로 항암활성을 규명하고자 in vitro에서 세포 독성능을 측정하여 다음과 같은 결과를 얻었다. HEP 2 cell에 대해 세포 독성능을 높게보인 생약제는 하고초>고삼>화누>선복화 순이었고, HeLa cell에 대해 세포독성능을 높게 보인 생약제는 선복화>아출>포공영>화누>구맥>하고초>고삼>오공>금은화>천문동 순 이었다. RAW 71 cell에 대해 세포독성능을 높게 보인 생약제는 구맥>화누>고삼>호모초>천문동 순 이었고, VERO E6 cell에 대해 세포 독성능을 높게 보인 생약제는 선복화>하고초 순 이었다. 또한 Herbal drug 처리에 의한 HEP 2와 HeLa 세포의 세포 독성능과 Staphylococcus aureus의 항균력간의 관계를 알아본 결과 HEP 2 cell은 약 43%, HeLa cell은 약 71%가 일치하였다. We tested the cytotoxic ability on the tumor cell line by 16 kinds of herbal drugs of which contains the alkaloids and flavoids which shows high antitumoral activity. This has been done on the condition of in vitro. the conclusion are as follows. 1. The herbal drugs which have shown the strong cytotoxic activity to HEP 2 cell are as follows. Prunella vulgaris has the strongest activity, followed by Sophorra angustifolia>Trichoosanthes kirilowii>Inula brontannaca. 2. The herbal drugs which have shown the strong cytotoxic activity to HeLa cell are as follows. Inula britannica has the strongest activity, followed by Curcuma zedoooaria>Tarazacum platycarpum>Trichosanthes kirilowii>Dianthus sinesis>Prunella vulgaris>Sophra angustifolia>Scolopendra subspinpes>Lonicera Japonica>Asparagus cochinensis. 3. the herbal drugs which have shown the strong cytotoxic activity to RAs 71 cell are as follows. Dianthus sinesis has the strongest activity, followed by Trichosanthes kirilowii>Sophra angustifolia>Corispermum stauntoni>Asparagus cochinensis 4. The herbal drugs which have shown the strong cytotoxic activity to VERO E6 cell are as follows. Inula britannica has the strongest activity, followd by Prunella vulgaris. 5. After the treatment with herbal drugs, the relationshipbetween the cytotoxic activity to HEP 2 and HeLa cell, the antimicrobial activity of Staphylococcus aureus was found. these two activites show the similarity of about 43% in HEP 2 cell and 71% in HeLa cell.

유산양 유방염에 대한 Chitosan 약침의 치료 효과

황수현,서진석,김덕환,김명철,신상태,전무형,南三郞,김영찬,이우근,표수일 충남대학교 수의과대학 동물의과학연구소 1999 動物醫科學硏究誌 Vol.7 No.-

To improve the therapeutic method for mastitis, treatment effect of chitosan pharmacopuncture was examined in three milking goats(A,B and C). Mastitis was artificially induced in two milking goats(A and B) and one goat(C) was naturally infected case with mastitis. The acupoint used was Yang-Ming and chitosan suspensions(provided from Tottory University, Japan: 30㎎ of chitosan/㎖)were injected to the acupoint with 5㎖/time for 3 days, respectively. As for the change of somatic cells in milk along with chitosan pharmacopuncture, they showed a decreasing tendency after increase on the 3rd day in all udders of B. In addition, as for the change of total leukocyte counts of peripheral blood with chitosan pharmacopuncture, they showed a decreasing tendency after increase on the 3rd day in B and C except A which showed a increasing tendency until on the 7th day. In the change of neutrophil/lymphocyte(N/L) ratio, increasing tendency was seen in A and C but increasing tendency after slight decrease was observed in B. Further, in the change of serum total protein and A/G ratio, serum total protein content was decreased after slightly increase on the 3rd day in A, and they were decreased after increase until on the 7th day in B and C, respectively. As for the change of A/G ratio, different pattern increasing tendency was shown in A, B and C.

무흡광색소 생물의 감광수용체 개발에 관한 연구(Ⅰ) : 표고버섯 중의 광감응성 mitochondrial ATPase Light-Induced Mitochondrial ATPase in the L. edodes (Berk) Sing

민태진,조석우,박상신 동국대학교 자연과학연구소 1987 자연과학연구 논문집 Vol.7 No.-

표고버섯의 미토콘드리아는 설탕 농도 44~50% 사이에서 분리 정제 되었다. 파장 변화에 따른 미토콘드리아성 ATPase의 비활성도는 680nm 파징에서 가장 증가하였다. 680nm에서 빛 조사 시간 변화에 따른 활성도는 5분 동안 조사 하였을 때 가장 증가 하였다. 최적 광조건(680nm 5분)에서 조사한 이 효소의 최적 pH 및 최적 온도는 7.5와 59℃였다. 최적 광조건에서 얻은 이 효소는 Fe³, Fe², Mg², K 및 Ca²이온에 의하여 활성화 되었고, Na이온에 의하여 억제 되었다. Mitochondria in the L. edades was purified by linear sucrose density gradient centrifugation. The mitochondrial ATPase activity was investigated by various wavelength illumination for 30 min at dark state. The mitochondrial ATPase activity was stimulated 1.6 fold by 680nm illumination compared with dark control group. The mitochondrial ATPase activity of different light illumination time at 680nm was stimulated 2.3 fold at 5minuted compared with dark control group. Its optimum pH and temperature were found to be 7.5 and 59℃ after illumination for 5 minutes at 680nm. The mitochondrial ATPaee activity was activated by 5 mmol Fe³, 0.1mmol Mg², 0.5mmol K, and 0.1mmol Ca²ion. But the enzyme was ingibited by 5mmol Na ion.

항암화학요법 중 호중구감소증이 발생한 저위험군 발열 환자들을 대상으로 한 경구 항균제 요법의 임상적 유용성 및 안정성에 대한 연구

김연숙,이혁,기현균,김춘관,김신우,김성민,백경란,김원석,윤성수,이홍기,강원기,박찬형,박근칠,송재훈 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

목적 : 항암화학요법 중 호중구감소증을 동반한 발열이 발생하는 암환자들을 치료하기 위한 다양한 항균제와 여러 가지 방법들이 시도되고 있는 가운데, 합병증과 사망률의 발생가능성이 적은 저위험군 환자들을 대상으로 초기 72시간동안 정주 항균제를 투여한 이후 경구 항균제로 전환하는 요법의 유용성과 안정성을 평가해보고자 본 연구를 시행하였다. 방법 : 1998년 2월부터 1999년 9월까지 본원에서 항암화학요법 중 호중구감소증과 발열이 발생한 환자들 가운데 기저 암질환이 고형암이거나 림프종이고, 입원당시 패혈증의 증후가 없으며 입원 72시간이내에 해열되고 백혈구수치가 증가 추세인 환자들을 대상으로 하여 72시간 동안 정주 항균제를 투여한 이후 경우 ciprofloxacin 750㎎을 하루 2회씩 투여하여 총 4일간 투여하였다. 모든 환자들은 열이 떨어지고 호중구감소증이 회복될 때까지 입원하도록 하였다. 결과 : 총 38명 환자의 40예가 등록이 되었고, 환자들의 기저암 질환은 고형함이 72.5%, 림프종이 27.5%였다. 입원당시 평균 호중구치수는 156/㎕였고, 호중구수치가 100/㎕미만인 경우는 65%였으며, 호중구감소증이 지속된 기간의 평균은 2.4일이었다. 40예 중 39예가 항균제의 변형이나 추가 없이 호중구감소증과 발열로부터 회복이 되어 97.5%의 성공율(95% 신뢰구간: 86.8-99.9%)을 보였다. 부작용으로 피부발진이 있었던 경우가 한 예 있었는데, 증상이 경하여 경구 항균제를 지속할 수 있었다. 심와부의 동통으로 복용을 지속할 수 없어서 대상에서 제외된 예가 또 한 예 있었다. 결론 : 항암요법 중 호중구감소증과 발열을 동반한 환자들 가운데 저위험군 환자들에서 항균제 72시간정주 이후 경구 항균제로의 전환요법은 효과적이고도 안전한 치료방법이라고 할 수 있다. Background : Oral antibiotic therapy following empirical intravenous antibiotics may be effective and safe for febrile neutropenic patients with lowrisk for complications. Methods : We conducted a prospective clinical trial of oral antibiotic therapy in the patients with neutropenia and fever during chemotherapy for cancer. Underlying malignancies were solid tumor or lymphoma with short duration of neurtropenia and the patients had no evidence of clinically or microbiologically documented infections. Oral ciprofloxacin was given to the patients who lacked signs of sepsis on admission, had a rising tendency of neutrophil count (ANC >100 /㎕ ) at 72 hours, and were afebrile at 72 hours. All patients were hospitalized until neutropenia and fever resolved. Results : A total of 40 episodes of 38 patients were enrolled from February 1998 to September 1999. The mean neutrophil counts on admission were 156/㎕ and the mean duration of neutropenia was 2.4 days. The episodes which had neutrophil count below 100 /㎕ were 26 (65%). Treatment was successful in 39 of 40 episodes (97.5% : 95 % confidence interval, 86.8% to 99.9%). Adverse reactions of oral ciprofloxacin were skin rash and epigastric soreness in two cases, respectively. There were no deaths during the study. Conclusions : For low-risk febrile patients with neutropenia during cancer chemotherapy, switch therapy to oral ciprofloxacin at 72 hours following intravenous broad-spectrum antibiotics is effective and safe,

국제학술 Symposium 논문집 : 생물학적동등성시험 실시 확대와 제약산업의 향후 전망

(Suck Woo Shin) 서울시립대학교 산업기술연구소 2002 국제학술SYMPOSIUM논문집 Vol.10 No.1

한국 Streptomyces sp. 로 부터 분리한 방향족 화합물과 지질 화합물의 세포독성 연구

신석우(Suck Woo Shin),염곤(Kon Ryeom) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.2

In an effort to screen new selective antitumor agents from the broth of soil microorganism, cytotoxicity oriented screening was performed against tumor cells and 3 compounds (Compound 1, 2 and 3) were isolated from Streptomyces parvullus ISP 5048 and their chemical structures were determined. Among these compounds, Compound 2 showed the highest cytotoxicity against P388D1 and L1210. While the IC_(50) values of compound 2 against P388D1 and L1210 were 0.073 ㎍/㎖ and 0.07 ㎍/㎖, respectively, and the IC_(50) value of Compound 3 was 0.17 ㎍/㎖ against human lung cancer cells, A549, the cytotoxicity of Compound 2 and 3 against normal cell line, Vero E6 cell was about 4- and 8-fold lower than that of adriamycin. Based on the chemical analysis data, Compound 3 was octacosamicine A, a known antibiotic, which was reported by Dobasih et al. (1988). Taken together the results demonstrated that Compound 2 and Compound 3 has the possibility to be developed as antitumor agent because of its potent cytotoxicity as well as high selectivity against various cancer cell lines.

폐어망 섬유를 혼입한 순환굵은골재 콘크리트의 특성에 관한 실험적 연구

신우철(Shin, Woo-Cheol),이호경(Lee, Ho-Kyung),백승민(Baek, Seung-Min),김우석(Kim, Woo-Suck),곽윤근(Kwak, Yoon-Keun) 대한건축학회 2016 대한건축학회 학술발표대회 논문집 Vol.36 No.2

순환굵은골재 고강도 철근콘크리트보의 최소철근비 평가

신우철(Shin, Woo-Cheol),이호경(Lee, Ho-Kyung),백승민(Baek, Seung-Min),김우석(Kim, Woo-Suck),곽윤근(Kwak, Yoon-Keun) 대한건축학회 2016 대한건축학회 학술발표대회 논문집 Vol.36 No.2

Nanocomposite scaffolds for myogenesis revisited: Functionalization with carbon nanomaterials and spectroscopic analysis

Shin, Yong Cheol,Song, Su-Jin,Shin, Dong-Myeong,Oh, Jin-Woo,Hong, Suck Won,Choi, Yu Suk,Hyon, Suong-Hyu,Han, Dong-Wook Informa UK (TaylorFrancis) 2018 Applied spectroscopy reviews Vol.53 No.2

<P>Skeletal muscle injuries are extremely common because skeletal muscle is quite frequently used in the human body, and these injuries can cause serious health implications. Currently, grafting and pharmacological therapies are the most common therapeutic methods for treating and repairing the skeletal muscle damages, but both therapeutic methods have significant limitations. Therefore, in recent years, the tissue engineering approaches have attracted much attention in biomedical and bioengineering fields. In particular, up-to-date studies have focused on the novel strategies aimed at promoting and enhancing the regeneration of skeletal muscle tissue by using tissue engineering scaffolds. Although the tissue engineering scaffolds can be readily fabricated with conventional biocompatible materials, such as polymer, ceramic, or metallic materials, the carbon nanomaterials (CNMs) are the most fascinating candidates as a scaffold material due to their favorable biocompatibility and extraordinary physicochemical, electronic, mechanical, and thermal properties. The aim of this review is to summarize some of the recent reports concerning the nanocomposite scaffolds functionalized with CNMs and to highlight promising perspective for the applications of CNMs as skeletal tissue engineering scaffolds. In addition, it is also discussed how the spectroscopic analysis can be employed for analyzing CNMs and nanocomposite scaffolds.</P>

Graphene-Based Nanocomposites as Promising Options for Hard Tissue Regeneration

Shin, Yong Cheol,Song, Su-Jin,Jeong, Seung Jo,Kim, Bongju,Kwon, Il Keun,Hong, Suck Won,Oh, Jin-Woo,Han, Dong-Wook Kluwer Academic Publishers 2018 Advances in Experimental Medicine and Biology Vol.1078 No.-