실험실탐방 : 민태진 교수 실험실

민태진 한국생화학분자생물학회 (구 한국생화학회) 1990 생화학분자생물학회 소식 Vol.10 No.3

누에의 非蛋白性 窒素物合化의 添食效果에 관한 硏究

閔泰鎭 동국대학교 1978 論文集 Vol.17 No.-

누에(水原 108×107號 交雜種)(Bombyx mori L.)에 3齡期에서 5齡期까지 尿素를 농도별(1,000ppm∼2,000ppm)로 添食시켜 성장도, 누에고치량의 變化, 蠶體內 Urease의 活性度, 核酸量의 變化, 蛋白質含量 및 蠶體內 蛋白質 전기이동像의 變化를 測定觀察하였다 成長增體量은 統計處理結果 U_3(1,400ppm)區와 U_4(1,600ppm)區가 對照區에 比하여 1% 高度有意性을 보였다. 成長增加率은 蠶品種에 따라 差는 있으나, U_3區와 U_4區에서 각각 16.60%, 17.43%로 U_4區가 最高値를 보였다. 누에고치 증가율은 U_3區와 U_4區가 각각 19.18% 및 20.11%를 보여 U_4區가 最高値였다. 蠶業의 利用效果面에서 尿素 1,400ppm∼1,600ppm으로 처리하는 것이 效果的이다. 蠶體內 Urease의 活性度와 그의 比活性度는 거의 같은 경향을 보이며, 3齡期에서 5齡期로 갈수록 活性度가 크게 감소하였다. Urease의 活性度는 처리 농도 증가에 따라 증가하지만, 1,600ppm以上에서는 감소하였다. 그러나 처리區는 對照區보다 감소하였다. Urease의 比活性度는 Urease의 活性度와는 달리 3齡期에서 對照區에 比하여 농도증가에 따라 증가하나 4齡期, 5齡期에서는 Urease의 活性度와 같은 경향을 보였다. 核酸量은 3齡期에서 5齡期에 갈수록 크게 감소하였고 처리區는 對照區에 比하여 크게 감소하다가 농도증가에 따라 증가하였다. 蛋白質含量은 Urease의 活性度 曲線과 같은 경향을 보였다. 5齡期 蠶體內 蛋白質 전기이동像은 對照區에서는 3個(D.E.F)의 전기이동像을, U_4區에서는 2個(Ⅳ, Ⅴ)의 전기이동像을 確認하였다. In this paper I tried to find the change of the growth rate, the weight of cocoon, and the change of urease activity, nucleic acid and protein contents, and the changes in electrophoretic patterns of protein in the silkworm (Suwon No. 108×107 confused species) (Bombyx mori L.) from the 3rd instar stage to the 5th instar stage by supplemental feeding of different concentrations of urea. Upon statistic analysis, the weight of increasing growth showed 1% high significant in U_3(1,400ppm) and U_4(1,600ppm) compared to the control group. In U_3 and U_4 which was treated with 1,400ppm and 1,600ppm urea respectively, the rate of growth (though varied with the species of silkworm) increased to 16.60% and 17.43% compared to the control groups, U_4 showing the highest rate. In U_3 and U_4, the rate of cocoon's weight increase showed 19.18%, 20.11% compared to the control groups, U_4 showing the highest rate. For the utilization of sericulture, the urea treatments of 1,400ppm to 1,600ppm are most effective. The activity or specific activity of urease in the silkworm between the 3rd instar stage and the 5th instar stage was almost same, but their activity was decreased sharply from the 3rd instar stage to the 5th instar stage. The activity of urease was increased with the increasing of the concentration of urea treatment, but not more than the control groups. Beyond U_4(1,600ppm) activity of urease was decreased gradually with the increasing of the concentration of urea. Unlike the urease activity, the specific activity of urease increased compared to the control group in the 3rd instar stage according to the concentration. But in the 4th and 5th instar stage both activities showed same tendency. The contents of nucleic acid was decreased sharply from the 3rd instar stage to the 5th instar stage. It was decreased sharply ar first in the treatment groups compared to the control group but afterward increased according to the concentration rate. The protein contents showed almost same curve as urease activity. In the 5th instar stage, electrophoretic patterns of protein in the silkworms were in three (D.E.F) the control groups and in two (Ⅳ, Ⅴ) U_4.

폐자동차 잔재물의 물질재활용을 위한 연구

민태진,윤진한,노선아,이정규,길상인,김우현 한국폐기물자원순환학회(구 한국폐기물학회) 2014 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2014 No.-

국내 “전기·전자제품 및 자동차의 자원순환에 관한 법률” 에서는 2014년까지 재활용율 85%(에너지 회수율 5%이내), 2015년부터는 95%(에너지 회수율 10%이내)를 목표로 하고 있으나 ASR의 재활용 설비가 거의 없기 때문에 ASR의 에너지 회수 및 ASR 내 함유된 철, 알루미늄, 구리 등의 유가 금속을 효과적으로 재활용하고 있지 못하는 실정이다. 우리나라에서 연간 폐차 발생량은 2009년을 기준으로 67만대에 달한다. 본 연구는 이러한 ASR의 효율적인 재활용을 위하여 ASR의 성형화를 위한 실험을 통하여 ASR을 균질화하고 보관이나 운반, 취급의 용이성을 높혀 연료로서 또는 물질재활용의 목적에 맞도록 전처리로서 성형화를 실시하였다. 성형설비는 시간당 1.5톤의 비중이 낮으면서 부피가 큰 ASR에 내구성을 가진 설비로 설계, 제작되어 실험에 사용되었다. 성형실험은 ASR의 성상별 혼합비, 노즐의 길이, 성형온도에 따른 성형효과와 처리 능력에 대하여 수행하였다. 실험 결과는 어떠한 첨가물이 없이 순수한 light fluff 와 heavy fluff만으로도 성형이 충분히 가능한 것을 알 수 있었다.

복령의 Carboxyl Proteinase 의 분리 정제 및 그 성질에 관한 연구 (2)

민태진,박상신,문순구 한국균학회 1986 韓國菌學會誌 Vol.14 No.2

The properties of carboxyl proteinase which was contained is Poria cocos (Schw.) Wolf were investigated by means of the purification with 0.65 ammonium sulfate saturation, DEAE-cellulose sad Sephadex G-75 gel filtration. This enzyme was found to hydrolyze only peptide bond between glutamyl-L-tyrosine of carbobenzoxy-L-glutamyl-L-tyrosine among the synthetic substrates of carbobenzoxy-L-glutamyl-L-tyrosine, hippuryl-L-phenylalanine and hippuryl-L-arginine. This enzyme was inhibited by Zn^(+2), Fe^(+2), Ca^(+2), CN^(-1), P₂O_7^(-1) ions, but stimulated by Hg^(+2) ion. Also, this enzyme was inhibited by organic compounds such as L-lysine, L-phenylalanine, hippuryl-L-phenylalaniae, diazoacetyl-DL-norleucine methyl ester (DAN) and 1.2-epoxy-3-(P-nitrophenoxy)propane(EPNP). In particular, the activity was inhibited by L-lysine till 20 minutes of preincubation time rapidly, and by DAN in the presence of Cu^(+2) ion more rapidly after 30 minutes than DAN in the absence of Cu^(+2) ion. L-Lysine was found to be a competitive inhibitor and its K_i value was determined to be 0.12 mmole by Dixon plot.

무흡광 색소 생물의 감광수용체 개발 연구 (3) - 표고버섯 중의 광감응성 Mitochondrial ATP Synthase -

민태진,이완기,박상신 ( Tae Jin Min,Wan Gie Lee,Sang Shin Park ) 한국균학회 1989 韓國菌學會誌 Vol.17 No.2

Ethrel의 植物體內 處理에 따른 잎 色素 含量 變化에 關한 硏究

閔泰鎭 東國大學校 1975 論文集 Vol.14 No.-

Ethrel 水溶液 500;ppm, 1,000ppm, 1,500ppm, 2,000ppm을 Group別 體內處理한 結果, 다음과 같은 結論을 얻었다. 1) 농도 증가에 따라 성장 억제를 받지만 1,000ppm의 경우는 억제를 가장 덜 받았다. 2) Total 잎色素의 含量은 1,000ppm 處理 group이 가장 적었다. 3) total 잎色素中에는 chlorophyll 含量이 가장 많고, Carotene의 含量이 가장 적었다. 4) Ethrel 농도 증가에 따라 Total 잎색소 中 各 成分의 含量은 감소하였다. 5) 다른 水溶性 有機體內 處理가 가능함을 알았다. As a result of an experimentation by the interior treatment (plant ; balsam) for Ethrel (2-chloroethyl phosphonic acid) aqueous solution per groups of 500ppm, 1,000ppm, 1,500ppm, 2,000ppm, the followings are conclusive findings: 1) As the concentration increased, the registance of the growth also increased. But in the case of 1,000ppm, the registance to growth was less intense. 2) The total content of leaf-pigment in the group of 1,000ppm was the least among others. 3) Among the three components of leaf-pigment, chlorophyll was the largest, carotene was the smallest, and xanthophyll was medium in the content. 4) As the concentration of Ethrel increased, the three components of leaf-pigment decreased in their content. 5) Thus we realized that water soluble organic chemicals can be interiorly treated.

권두언

민태진 생화학분자생물학회 1992 생화학분자생물학회 소식 Vol.12 No.3

한국산 버섯추출물의 항진균 및 항세균활성 검색 (2)

민태진,김은미,유선호 ( Tae Jin Min,Eun Mi Kim,Sun Hoo You ) 한국균학회 1996 韓國菌學會誌 Vol.24 No.1

백복령 Whit Poria Cocos(Schw.) Wolf중의 지방산 성분에 관한 연구

閔泰鎭,文順九 동국대학교 대학원 1986 大學院硏究論集-東國大學校 大學院 Vol.16 No.-

The quantitative analysis of phospholipids, sterols, proteins, free sugars and fatty acids in Korean White Poria cocos(Schw.) Wolf were performed. Dry powder of sample was extracted with chloroform : methanol(2 : 1 v/v) mixed solvent. The extract was saponified and methylated, and then analyzed by gas chromatograpy. Total 24 peaks were observed and 5 peaks i.e., C_(8:0); caprylic acid, C_(11:0); undecanoic acid, C_(12:0); lauric acid, C_(12:1); dodecenoic acid, C_(16:0); palmitic acid were identified. Major components were C_(8:0) and C_(12:1) and were determined as 6.657ug and 10.176ug pergam, respectively. Total phospholipids, sterols, proteins and free sugars were determiened 3.233mg, 0.507mg 7.644mg and 2,420mg per gram, respectively.

Purification and Properties of Carboxyl Proteinase from the Poria cocos (SchW.) Wolf (I)

민태진,정광식,김재웅,Min, Tae-Jin,Chung, Kwang-Sik,Kim, Jae-Woong 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.3

복령[Poria cocos (Schw.) Wolf.]중의 carboxyl proteinase을 황산 암모늄 65% 포화용액, DEAE-cellulose 및 Sephadex G-75을 이용한 gel-filtration 방법으로 분리 정제하여 그 성질을 조사하였다. 이 버섯중에서 활성분획분 I과 II을 얻었으며, 활성분획분 II에 대하여 bovine hemoglobin을 기질로 사용하여 얻은 carboxyl proteinase의 활성도의 최적 pH는 2.0, 최적온도는 $70^{\circ}C$, pH 안정성은 1.2~2.5 및 열안정성은 $25^{\circ}C{\sim}75^{\circ}C$였다. 이 효소의 subunit는 1개였고, 18종의 아미노산으로 구성된 단백질이였으며, 겉보기 분자량은 25,500(PAGE)~23,000 (HPLC)였다. 이 효소는 carbobenzoxy-L-glutamyl-L-tyrosine (CBZ-glu-tyr.)의 glutaml-L-tyrosine간의 부위를 절단하는 특이성을 가졌었다. CBZ-glu-tyr.을 사용하여 측정한 Km 값은 0.29 mM 이였다. The enzymic properties of carboxyl proteinase isolated from the Poria cacos (Schw.) Wolf. were investigated. Two kinds of active fraction I and II were isolated from this mushroom. The active fraction II showed carboxyl proteinase activity to bovine hemoglobin substrate, and its optimum conditions were the followings; temperature, $70^{\circ}C$, pH stability, 1.2~2.5 and thermal stability, $25^{\circ}C{\sim}75^{\circ}C$. The enzyme appeared to be one subunit, and consisted of 18 kinds of amino acids. The apparent molecular weight were 25,500 (PAGE) and 23,000(HPLC), respectively. The enzyme appeared to hydrolyze peptide bond between glutaml-L-tyrosine. The Km value of this enzyme was 0.29mM when carbobenzoxy-L-tyrosine were used as a subtrate.