급성췌장염에 의한 췌장가성남종에서 내시경적 배액술후 발생한 췌장가성남종과 십이지장 및 대장에 발생한 내부 췌장루 1예

김연수,이문성,김진오,조영덕,이영홍,봉형근,조주영,이준성,심찬섭,황성규,전기원,변재호,장재영 대한소화기학회 1996 대한소화기학회지 Vol.28 No.1

중등도 및 중증고혈압에서 Nifedipine 설하투여의 강압효과에 관한 연구

김영훈,서순규,최인석,김열홍,안희권,심완주,노영무 대한내과학회 1986 대한내과학회지 Vol.31 No.5

In order to assess the efficacy of sublingual nifedipine in the acute treatment of moderate to severe hypertension, a single blind test was done in 30 patients, 10 males and 20 females, aged 40 to 76 years, with moderate to severe hypertension. The patients with mean sitting blood pressure(BP) of more than J40 mmHg received sublingual placebo, and BP and pulse rate(PR) were measured in sitting position 10,20 and 30 minutes after the sublingual placebo was given. The patients with post-placebo 30 minutes sitting mean BP of less than 13pmmHg were excluded from the study. The placebo treatment period was followed by the administration of 10mg of sublingual nifedipine, and BP and PR were measured 10,20,30 and 45 minutes after nifedipine was given. BP decreased with placebo treatment(p$lt;0. 01 by paired t-test, preplacebo vs. post-placebo 30 minutes). With sublingual nifedipine BP started to fall in 10 minutes and reached the lowest levels in 30 or 45 minu1es. The systolic BP decreased from 198.0±21.4mmHg to 159±20.4mmHg, diastolic BP from 122.5±13.0 mmHg to 103.2±14.4 mmHg and mean from 147.7±13.4 mmHg to 121.7±14.8 mmHg 45minutes after nifedipine(p$lt;0.01 by paired t-test). The average fall in systolic BP/diastolic BP/mean BP 45 minutes after nifedipine was 39/19/26 mmHg(p$lt;0. 001, by paired t-test). The maximal fall in systolic BP correlated better with pretreatment value(r=0.42) than the maximal fall in diastolic pressure(r=0.21), PR increased from 79±12 to 87±11/minute with nifedipine(p$lt;0.001, by paired t-test). Side effects were mild facial flushing(16.7%) and palpitation (3.3%). The findings suggest that a dose of 10mg of sublingual nifedipine is a simple, effective and safe method for the acute treatment of moderate to severe hypertension and can be used as a non-injecting alternative agent for the treatment of hypertensive emergency.

성인에서 발견된 십이지장 막양구조

박경규,박형근,김연수,이문성,김진오,조영덕,이영홍,봉형근,조주영,이준성,심찬섭,황성규,함정식,장재영,권귀향 대한내과학회 1997 대한내과학회지 Vol.53 No.2

Duodenal obstruction is the third leading cause of intrinsic obstruction of the alimentary tract in the newborn, the other causes are imperforate anus and esophageal atresia. If the obstruction is incomplete, as with a fenestrated duodenal membrane, the presentation of the symptoms may be delayed and the diagnosis overlooked. And so they are rarely diagnosed in the adult. Diagnosis can be made by contrast duodenography but is more definitive with endoscopy. This is the first case report of a 21-year-old female with duodenal membrane, associated with incomplete rotation of intestine and uterus bicornis, diagnosed by hypotonic duodenography and endoscopy, preoperatively, and successfully treated with duodenoduodenostomy.

안전 출입 시스템을 위한 체적 홀로그래픽 광지문인식

이승현,박민순,심원섭 한국산업안전학회 1999 한국안전학회지 Vol.14 No.4

본 논문에서는 정합필터의 데이터 베이스로 체적 홀로그램을 사용한 광 지문 인식 시스템을 제안하였다. VanderLugt 상관기에 사용되는 정합필터는 방대한 저장 능력과 실시간 랜덤 엑세스의 장점을 갖는 체적 홀로그램에 기록된다. 데이터 베이스로 사용되는 지문 영상들은 각각 공간광변조기에 디스플레이 되어 퓨리에 변환 렌즈를 통과한 후 퓨리에 변환된 공간정합필터의 형태로 광굴절매질에 기록된다. 대용량의 테이터 베이스를 구성하기 위한 다중화 방법으로 스텝 모터를 이용하여 기준파의 각도를 회전하는 각다중화 기술을 사용하였다. 실험을 퉁해 본 시스템이 제한된 영역의 접근을 통제하기 위한 개인 인증용 안전 출입 시스템 및 크레디트카드, 여권, ID등의 위조방지에 이용이 가능함을 제시하였다. We propose an optical fingerprint identification system using volume hologram for database of matched filter. Matched filters in VanderLugt correlator are recorded into a volume hologram that can store data with high density, transfer them with high speed, and select a randomly chosen data element. The multiple reference fingerprint photographs of database are prerecorded in a photorefractive material in the form of Fourier transform images, simply by passing the image displayed in a spatial light modulator through a Fourier transform lens. The angular multiplexing method for multiple holograms of database is achieved by controlling the reference directions with a step motor. Experimental results show that the proposed system can be used for secure entry systems to identify individuals for access to a restricted area, security verification of credit cards, passports, and other IDs.

Effective thermal conductivity of MgH₂ compacts containing expanded natural graphite under a hydrogen atmosphere

Shim, J.H.,Park, M.,Lee, Y.H.,Kim, S.,Im, Y.H.,Suh, J.Y.,Cho, Y.W. Pergamon Press ; Elsevier Science Ltd 2014 International journal of hydrogen energy Vol.39 No.1

Expanded natural graphite (ENG) was added to enhance the effective thermal conductivity of MgH<SUB>2</SUB>, which is one of the important parameters in the design of MgH<SUB>2</SUB>-based hydrogen storage tanks. Cylindrical MgH<SUB>2</SUB> compacts containing up to 20 wt% ENG flakes with various average sizes (20, 50, 200, 350 and 1200 μm) were fabricated to measure the effective thermal conductivity of MgH<SUB>2</SUB>-ENG mixtures. The radial direction effective thermal conductivity of the compacts was measured under a hydrogen atmosphere up to 70 bar. The conductivity was significantly enhanced by the addition of ENG flakes, reaching 9.3 W m<SUP>-1</SUP> K<SUP>-1</SUP> at 20 wt% ENG at 1 bar of hydrogen. It was observed that hydrogen pressure and the size of ENG flakes influenced the conductivity together with the amount of ENG. As hydrogen pressure increased up to 20 bar, the conductivity continued to increase. On the other hand, the conductivity very slowly increased above 20 bar, exhibiting a saturation tendency. It relatively rapidly increased with increasing average flake size up to 200 μm and then gradually decreased with further increasing size up to 1200 μm, exhibiting the maximum value at an average flake size of 200 μm. This trend might be determined by the competition between the thermal resistance at ENG/MgH<SUB>2</SUB> interfaces and the formation of conductive networks of ENG flakes.

Tectoridin from Maackia amurensis modulates both estrogen and thyroid receptors

Shim, M.,Bae, J.Y.,Lee, Y.J.,Ahn, M.J. G. Fischer 2014 Phytomedicine Vol.21 No.5

<P>Aim: The stem bark of Maackia amurensis has been used as folk medicine for the treatment of cancer, cholecystitis, arthritis, and hyperthyroidism in females. In this study we examined the effects of the ethyl acetate fraction obtained from the 70% ethanol extract of M. amurensis and tectoridin, an active constituent isolated from the ethyl acetate fraction on thyroid and estrogen hormone activity. Methods: The effect of the ethanolic extract of M. amurensis stem bark on thyroid hormone activity was evaluated using thyroid hormone responsive-luciferase assay. We isolated tectoridin from the ethyl acetate fraction using a recrystallization method. T-screen assays were used to confirm thyroid hormone activity. The estrogenic activity of the ethyl acetate fraction of M. amurensis and tectoridin was evaluated by estrogen responsive-luciferase assay and estrogen receptor alpha regulation as compared to 17 beta-estradiol. Results: Both the ethyl acetate fraction and tectoridin activated thyroid-responsive reporters and increased thyroid hormone-dependent proliferation of rat pituitary GH3 cells, indicating modulation of thyroid hormone receptors. In parallel, the estrogenic activity of the fraction and tectoridin were characterized in a transient transfection system using estrogen-responsive luciferase plasmids in MCF-7 cells. The ethyl acetate fraction and tectoridin activated reporter gene expression and decreased the estrogen receptor protein level. Conclusions: These data indicate that tectoridin acts as a weak phytoestrogen as well as a thyroid hormone like agent by activating both estrogen and thyroid hormone receptors. (c) 2013 Elsevier GmbH. All rights reserved.</P>

Supplementation of oil-based inactivated H9N2 vaccine with M2e antigen enhances resistance against heterologous H9N2 avian influenza virus infection

Park, J.K.,Lee, D.H.,Cho, C.H.,Yuk, S.S.,To, E.O.,Kwon, J.H.,Noh, J.Y.,Kim, B.Y.,Choi, S.W.,Shim, B.S.,Song, M.K.,Lee, J.B.,Park, S.Y.,Choi, I.S.,Song, C.S. Elsevier Scientific Pub. Co 2014 Veterinary microbiology Vol.169 No.3

Avian influenza virus (AIV) subtype H9N2 has been evolving rapidly and vaccine escape variants have been reported to cause circulation of infections and economic losses. In the present study, we developed and evaluated ectodomain of the AIV matrix 2 (M2e) protein as a supplementing antigen for oil-based inactivated H9N2 vaccine to increase resistance against vaccine escape variants. AIV H9N2 M2e antigen was expressed in Escherichia coli and supplemented to inactivated H9N2 oil emulsion vaccine. Specific pathogen-free chickens received a single injection of inactivated H9N2 oil emulsion vaccines with or without M2e supplementation. At three weeks post vaccination, hemagglutination inhibition tests and enzyme-linked immunosorbent assays were performed to determine serological immune responses. Challenge study using a vaccine escape H9N2 variant was performed to evaluate the efficacy of M2e supplementation. M2e antigen supplemented in oil emulsion vaccine was highly immunogenic, and a single M2e-supplemented vaccination reduced challenge virus replication and shedding more effectively than non-supplemented vaccination.

Quantum dot nanoprobe-based high-content monitoring of notch pathway inhibition of breast cancer stem cell by capsaicin

Shim, Y.,Song, J.M. Academic Press 2015 Molecular and cellular probes Vol.29 No.6

Breast cancer is the major cause of cancer death for women worldwide. Breast cancer patients are treated with chemotherapy and radiotherapy. Although chemotherapy and radiotherapy are applied, some cancer cells still survive. These cells, called cancer stem cell (CSC), exhibit special capabilities, such as drug and radio resistance. The remaining CSC can trigger cancer recurrence. Thus, it is critical to find an effective way to target CSC. Capsaicin has been reported to affect anticancer activity in many cancers. It also has been shown that capsaicin induces apoptosis in the MCF-7 breast cancer cell line. In this study, we demonstrate that capsaicin causes dose-dependent growth disruption in breast CSC and inhibits translocation of notch intracellular membrane domain (NICD) into the nucleus. MCF-7 cells were treated with capsaicin at various concentrations (5 μM, 10 μM, and 20 μM) for 24 h. After capsaicin treatment, it was found that the number of breast CSC (%) decreased as the treatment concentration of capsaicin increased. This result was also confirmed with FACS. NICD translocation to the nucleus and apoptotic cell death of breast CSC were concurrently observed at the single breast CSC level using highly sensitive quantum dot (Qdot)-antibody nanoprobes. The control breast CSCs without the capsaicin treatment were able to translocate NICD into the nucleus. On the other hand, translocation of NICD into the nucleus was not observed in capsaicin-treated cells. In addition, apoptotic cell death was caused when the breast CSC were treated with capsaicin at more than 10 μM. Although many studies have shown that capsaicin produces anticancer activity in cancer cell lines, the present result is the first report to demonstrate that capsaicin is capable of causing breast CSC apoptotic cell death via inhibiting its notch signaling pathway.

Spectral and Thermal Studies of Transition Metal PSSA Ionomers

Shim, Il-Wun,Risen, William M. Jr. Korean Chemical Society 1988 Bulletin of the Korean Chemical Society Vol.9 No.6

Transition metal PSSA ionomers containing Co(II), Ni(II), Cr(III), Ru(III), and Rh(III) are investigated by IR, Far-IR, UV-Vis and DSC. Reliable IR Spectroscopic criteria are established for assessing the degree of ion-exchange of PSSA ionomers and the local structures around metal cations in them. In the hydrated transition metal PSSA ionomers, the ionic groups are solvated by water molecules and there is no significant interactions between sulfonate group and metal cations. The visible spectra indicated that metal cations are present as [M$(H_2O)_6$]$^{n+}$ with Oh symmetry. Their $T_g$ values increase as the extent of ionic site concentration increases, but there is no direct dependence of $T_g$ on the nature of metal cations or their oxidation states. Thus, the water content in PSSA ionomer is found to have dominant influence on $T_g$ of hydrated transition metal PSSA ionomers. Dehydration of the hydrated transition metal PSSA ionomers results in direct interaction between ionic groups and significant color changes of the ionomers due to the changes of the local structures around metal cations. On the base of spectral data, their local structures are discussed. In case of dehydrated 12.8 and 15.8 mol % transition metal PSSA ionomers, no glass transition is observed in 25-$250^{\circ}C$ region and this is believed to arise from the formation of highly crosslinked structures caused by direct coordination of sulfonate groups of metal cations. In the 6.9 mol % transition metal PSSA ionomers, the glass transition is always observed whether they are hydrated or dehydrated and this is though to be caused by the sufficient segmental mobility of the polymer backbone.

Chemiluminescence competitive aptamer assay for the detection of aflatoxin B1 in corn samples

Shim, W.B.,Mun, H.,Joung, H.A.,Ofori, J.A.,Chung, D.H.,Kim, M.G. Butterworths ; Taylor Francis ; Elsevier Science 2014 FOOD CONTROL Vol.36 No.1

In this study, we developed a chemiluminescence competitive aptamer assay for aflatoxin B1 (AFB1) using a hemin/G-quadruplex horseradish peroxidase-mimicking DNAzyme (HRP-DNAzyme) linked with an aptamer specific to AFB1. Single, double, and triple HRP-DNAzymes coupled to the AFB1 aptamer were tested, and the AFB1 aptamer linked with double HRP-DNAzymes that produced sufficient chemiluminescence (CL) values when binding to AFB1-ovalbumin (OVA) used as a coating antigen, was selected. Under conditions optimized by testing key parameters, the aptamer assay exhibited a wide dynamic range from 0.1 to 10 ng/mL and showed a limit of detection of 0.11 ng/mL. Cross-reaction to aflatoxin G1, aflatoxin M1, and zearalenone was observed but no cross-reaction to other mycotoxins or the herbicide (atrazine) was observed. Aqueous methanol (20%) gave a good extraction efficiency and the matrix influence from corn extracts was successfully reduced through 4-fold dilution with water. Recovery from spiked corn samples averaged from 60.4 to 105.5%. Thus, the aptamer linked with HRP-DNAzymes can be useful as a reagent in the development of a biosensor for the rapid and simple detection of AFB1. Results from this study provide the basis for research into the development of various aptasensors for AFB1 analysis in foods.