RAPD법을 이용한 양파품종(Allium cepa L.)간의 유전변이와 유연관계분석

양보경,김도훈,이영병,남재성,정순재 동아대학교 농업생명과학연구소 2001 農業生命資援硏究 Vol.10 No.1

This study was carried out to investigate genetic variation and relationships among onion varieties, Allium cepa L., by RAPD-PCR analysis. In the test of 120 primers with 58 onion varieties collected from local and abroad, eight primers turned out to be useful for further analysis. These primers eventually provided a total of 71 bands, consisting of 68 polymorphic and three monomorphic ones. The polymorphic bands were subjected to phylogenetic analysis using NTSYS and PAUP computer programs. In the NTSYS analysis, similarity coefficient of the 58 onion varieties ranged from 0 to 0.9179 and that of 27 varieties with obvious origin ranged from 0.0306 to 0.9032, The dendrogram of 58 varieties obtained by NTSYS analysis resulted in 15 groups, but overall groupings were not consistent with bulb phenotypes (e.g., color, weight, and shape) or origin of the varieties either In the PAUP analysis, genetic divergence of the 58 onion varieties ranged from 0.042 to 0.532 and that of 27 varieties with obvious origin ranged from 0.042 to 0.394. Phylogenetic analysis using PAUP revealed 13 group with 58 varieties and nine group with 27 varieties, but these were not consistent with any bulb phenotypes or origin either, as seen in the NTSYS analysis.

신장에 발생한 섬유육종 1례

이재규,손경민,권동득,오봉렬,류수방,박양일,최찬 大韓泌尿器科學會 2001 Korean Journal of Urology Vol.42 No.7

3D Dental-CT를 이용한 상악 매복 과잉치의 진단 : 증례보고

김재곤,김수경,양연미,백병주,김성희 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

과잉치는 법랑기(enamel organ)가 치배형성을 지속하거나 과도한 세포증식을 함으로써 발생한다. 과잉치는 상악 전방부에서 종종 발견되며, 유치열기와 혼합치열기에서 다양한 문제를 야기한다. 과잉치는 형태에 따라서 보충치(supplemental tooth)와 흔적치(rudimental tooth)로 나눌 수 있다. 보충치는 정상 절치와 크기 및 형태가 유사하나, 흔적치는 비정삭적인 형태와 작은 크기를 갖고 있다. 보충치는 영구 측절치 부위에서 호발하며 정상 전치와 유사한 형태와 크기를 갖고 있으므로, 발치시 주의를 기울여야 한다. 본 3증례는 상악 전방부에 정상 전치 형태의 과잉치를 갖는 증례들이다. 모든 증례에서, 치근단, 교합, 파노라마 방사선 사진 등의 기존 방사선 사진 촬영 외에 3Dimensional Dental-Computed Tomography(3D Dental-CT)를 촬영하여 진단하였다. 기존 일반 방사선 사진들(conventional radiographs)과 3D Dental-CT를 비교시, 일반 방사선 사진들은 2차원적인 영상만을 제공하여 상의 해석에 있어서 술자의 주관이 개입되는 한계가 있다. 반면 3D Dental-CT는 과잉치의 정확한 위치와 형태에 대한 객관적인 정보를 제공하며, 좀 더 보존적인 수술을 시행하고, 수술 시간과 스트레스를 줄이는데 유용하다. 그러므로 일반 방사선 사진 촬영 후 과잉치의 위치가 불분명하고 인접 영구치의 손상이 의심되는 경우 3D Dental-CT를 이용한 진단이 고려된다. Supernumerary teeth are teeth which result from the continued budding of the enamel organ of the preceding tooth or from excessive proliferation of cells. They are most often found in the maxillary anterior region. And they can be responsible for a variey of irregularities in the primary and transitional dentition. There are two morphological types of supernumerary teeth: supplemental and rudimentary. Supplemental teeth have normal shape and size. In contrast, rudimental teeth have abnormal shape and smaller size. Supplemental supernumerary teeth are most common in permanent lateral incisor area. Its extration must be decided more carefully with differential diagnosis between normal teeth, because it has nomal shape and size. We reports 3 cases of the normal incisor shaped teeth in the maxillary anterior region. In all cases, we used the 3D Dental-CT as well as the conventionl plain film such as periapical, occlusal, and panoramic ridiograph. Consequently, 3D Dental-CT was valualble to figure out the exact position and morphology of supernumerary teeth, to do more conservative surgery, and to reduce surgery stress and time.

제1대구치의 석회화와 맹출 양상

정해경,양연미,김재곤,백병주,소유려 大韓小兒齒科學會 2010 大韓小兒齒科學會誌 Vol.37 No.3

Among the permanent teeth, the first permanent molars play the greatest role in occlusion and function. So, early diagnosis for congenital missing, abnormal eruption and abnormal formation is very important to the first permanent molars in the course of arch development. The aim of this study is to analyse the differences between right and left first permanent molar's formation and eruption and between upper and lower one. A total of 545 children were selected am ong children who had visited our clinic, investigate eruption and calcification stage of permanent first molar, based on Gleiser and Hunt criteria for this study. 1. Gingival emergence of mandibular first molar is faster than maxillary first molar by 0.75~0.8 years, gingival emergence of maxillary first molar in girls faster than boys by 0.45 years, and that of mandibular first molar in girls faster than boys by 0.5 years. 2. There is the significant difference between right and left first molar on the eruption score and the calcification stage ; 5 year old children show the significant difference on the eruption score. 7 year old children show the significant difference on both the eruption score and calcification stage. 3. It shows the most active eruption movement of crown on the maxilla while the root is rapidly widening its furcation and completing root formation to 2/3, on the other hand, the most active crown emerging on mandible is shown when the root formation completed to 1/4 to 1/2. 제1대구치는 치열의 교합 및 발육에 중요하게 관여하며 기능적, 형태적으로 건전한 교합의 발육 및 유지에 필수적인 치아이다. 치열의 발육과정에 있어서 제1대구치의 형성, 맹출 및 형태의 이상이나 선천결손의 발생여부를 조기에 진단하는 것은 매우 중요하다. 이 연구의 목적은 제1대구치의 맹출과 석회화 양상에 대해 조사하여 형성과 맹출의 상, 하악과 좌, 우 차이에 대해 평가하는 것이다. 2007년 7월부터 2008년 6월까지 전북대학교 치과병원 소아치과에 내원하여 파노라마 방사선사진을 촬영한 545명의 어린이를 대상으로, 제1대구치의 맹출 위치와, Gleiser and Hunt의 석회화단계의 분류를 이용하여 제1대구치의 석회화에 대해 연구한 결과 다음과 같은 결론을 얻었다. 1. 제1대구치의 구강내 출은은 하악 제1대구치의 구강내 출은이 상악 제1대구치의 출은보다 0.75~0.8년 빠르게 일어났으며, 여성은 남성에 비해 상악은 약 0.45년, 하악은 약 0.5년 먼저 출은했다. 2. 제1대구치의 석회화단계와 맹출 정도의 좌, 우 차이는, 5세에서 상악 좌, 우측 맹출 정도와, 7세에서 상악 좌, 우측 맹출정도, 하악 좌, 우측 석회화단계가 유의한 차이를 보였다. 3. 상악은 치근 분지부의 급속한 확대단계와 치근 2/3완성단계 사이에서, 하악은 치근 1/4완성단계와 치근 1/2완성단계 사이에 가장 많은 맹출 이동량을 보였다.

자가이식을 이용한 상악 매복 견치의 치료 : OF IMPACTED MAXILLARY CANINES

김수경,백병주,김재곤,양연미 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.3

적외선 열화상 카메라를 이용한 Plume 형상에 따른 Nd:YAG 레이저 용접 결함 평가

김재열,유영태,양동조,송경석,노경보 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

In Nd:YAG laser welding, evaluation methods of welding flaw are various. But, the method due to plume shape is difficult to classification of welding flaw. The Nd:YAG laser process it known to have high speed and deep penetration capability to become one of the most advanced welding technologies. At the present time, some methods are studied for measurement of plume shape by using high-speed camera and photo diode. This paper describes the machining characteristics of SM45C carbon steel welding by use of an Nd:YAG laser. In spite of its good mechanical characteristics, SM45C carbon steel has a high carbon contents and suffers a limitation in the industrial application due to the poor welding properties. In this study, plume shape was measured by infrared thermal camera that is non-contact /non-destructive thermal measurement equipment through change of laser generating power, speed, focus. Weld was performed on bead-on method. Measurement results are compared as two equipments. Here, two results are composed of measurement results of plume quantities due to plume shape by infrared thermal camera and inspection results of weld bead include weld flaws by ultrasonic inspector.

선천성 결손치에 관한 임상적 연구

정해경,양연미,김재곤,백병주,정진우,김하나,김미아 大韓小兒齒科學會 2009 大韓小兒齒科學會誌 Vol.36 No.2

The congenital missing of teeth is common, which takes place since the proliferation and differentiation are not allowed in that tooth bud fail to start development. The purpose of this study is to research incidence rate, number, and missing part of congenital missing teeth, and to study whether a person who has missing teeth has other abnormality of teeth or not. For this study, 1,520 subjects(aged 2.9~17) who had visited pediatric dentist department of Chonbuk national university dental hospital within 2 years were examined with an panoramic radiograph ; exempting third molar missing state. The obtained results are as follows. 1. 8.88% among total subjects show missing teeth ; male 9.05%, female 8.64% 2. The most frequently missing permanent teeth were the mandibular second premolars(22.3%). The most frequently missing primary teeth are mandibular lateral incisors(50%). 3. 43.3% patients have one permanent missing tooth, 34.3% have two, and 10.4% have more than six, respectively. In primary teeth, 86.7% patients have one missing tooth, and 13.3% have two missing teeth. 4. 18 patients(13.3%) have missing teeth as well as hyperdontia, while some patients have microdont, ectopic eruption, and fusion teeth. 치아의 선천성 결손은 치배의 발육이 시작되지 못하여 치아의 증식, 분화가 일어나지 못함으로서 발생하는 흔한 치아의 발육 이상이다. 이 연구의 목적은 선천성 결손치의 발생률, 결손치의 수, 발생부위와 결손치 환자에게서 나타난 다른 치아이상의 발생여부를 조사하는 것이다. 본 연구는 2006년 7월부터 2008년 6월까지 전북대학교 소아치과에 내원하여 파노라마를 촬영한 1,520명의 환자(2.9~17세)를 대상으로 제 3 대구치를 제외한 선천성 결손치의 분포를 조사하여 다음과 같은 결과를 얻었다. 1. 전체 1,520명 중 총 8.88%에서 결손치가 관찰되었으며, 남자가 9.05%, 여자가 8.64%에서 결손치가 관찰되었다. 2. 총 350개의 영구 결손치 중 하악 제 2 소구치가 22.3%로 가장 발생률이 높았고, 하악 측절치, 상악 제 2 소구치, 하악 중절치, 상악 측절치 순이었다. 또한 총 18개의 유치 결손치 중 하악 유측절치가 가장 높은 발생률(50%)을 보였다. 3. 1개의 영구치 결손치를 가지는 환자는 43.3%, 2개는 34.3%, 3개는 6,7%, 4개는 1.5%, 5개는 3.7%, 6개 이상은 10.4%이었다. 또한 유치 결손치의 수는 1개는 86.7%, 2개는 13.3%이었다. 4. 결손치가 있는 환자 중 과잉치를 가지는 환자는 18명(13.3%)이 있었으며, 왜소치, 이소맹출, 융합치 등의 치아이상을 지닌 환자도 관찰되었다.

콩 이소플라본이 성장기 쥐의 골대사에 관한 연구

김영경,심재영,이헌옥,양승오,엄애선 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.2

Soybean has been consumed in Asia as a lot of soyfood products. Soy isoflavones, particularly genistein. have preventive effect on breast and prostate cancer, atherosclerosis and osteoporosis. To investigate if the influence of soy isoflavones on bone metabolism we used 3-week- old female Wister Rat ovariectomized. All the rats were divided into sham (SH) , ovariectomized (OVX) , OVX- 17β-estradiol (10 ㎎/㎏ b.w.), OVX-genistein (5 ㎎/㎏ b.w.). They were freely fed foods and drinking water for 8 weeks. Results show that body weight had significantly decreased in SH group, compared to OVX groups. The serum alkaline phosphatase (ALP) was increased in SH, OVX-GEN and OVX-ES groups but not significant. Creatinine and hydroxyproline level lowered in SH group but not significant. Femur, feces, serum and urinary Ca level was significant and femur, serum P level did not differ. Bone mineral density (BMD) decreased OVX group but increased slightly isoflavone group. It suggests that estrogen may affect bone mineralization in growing rats and soy isoflavones may involve prevention of bone loss. More studies are needed to identify the mechanism of soy isoflavones and bone formation.

초등학교 과학 수업에서 팀 게임 토너먼트(TGT) 협동학습의 효과

고한중,양수경,한재영,노태희 한국초등과학교육학회 2003 초등과학교육 Vol.22 No.3

The purpose of this study was to develop a way of using Teams Games Tournaments that fits in elementary school science instruction, and to find out its effects on student's learning. Three classes (103 sixth graders) were divided into three groups; a traditional learning group, a cooperative learning group, and a teams games tournaments cooperative learning group. Students were taught about 'several kinds of gas' for nine class periods. The instructional effects upon student's achievement, science learning motivation and self-esteem toward science instruction were examined. The result of this study indicated that there was a significant interaction effect between the treatment and achievement level in the achievement test scores. The achievement test scores of the low level students were significantly higher in the teams games tournaments cooperative learning group than those in the traditional learning groups.

朝鮮後期 寺刹 寮舍空間의 特性과 變化에 관한 硏究 : 主佛殿 앞마당에 面한 寮舍를 中心으로 Focused on the Yosa faced with the front court of a Central Buddhist Sanctum

김종헌,김도경,양재영 대한건축학회 2003 대한건축학회논문집 Vol.19 No.1

Yosa is a space for organizational living of buddhist monk that is composed of daebang, kitchen, gidaebang, rooms, storages, and so on. The plan of Yosa forms shape of ㅡ,ㄱ,ㄷ,ㅁ. Yosa is organized by practical aspect that is based on living transformation, and each space has its own usual order. Daebang is a centered space that connects the holy space with the mundane space in Yosa. The use and layout of kitchen has intimate relation with constitution of the central space in buddhist temple. As a result of the investigation of the characteristic and transformation of Yosa that is faced with the front court of a central buddhist sanctum, we can understand the space composition principle of the buddhist temple in the Chosun Dynasty. The existing sanctum-centered researches in buddhist temple layout must go side by side with diversified studies.