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박히준,채윤병,차웅석,박종배,이혜정,이향숙,인창식,고형균,김수영,최일환,김강식,문정배,배기태,유경환,육근영,정병주,손인철,임사비나 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-
Background : The cun measurement System, an essential and convenient method in locating acupoints, has been widely used in the practice of acupuncture. However, traditional cun measurement has been criticized for its lack of reliability. Objectives : The purposes of this study are to determine if one cun measured by the directional methods have a consistency with that of proportional methods and to investigate which factors are related with these differences, especially in forearm. Methods : The distance between the elbow crease and the wrist crease of forearm was compared to a reference value of one cun obtained by the directional method. In this method, one cun is one third of the distance between index finger and Small finger of a subject, measured at proximal interphalangeal joint. In addition, to investigate the factors influencing the differences between these two methods, we measured the height and body weight and calculated body mass index (BMI). Finally we analyzed the factors correlated with these lengths by linear regression test. Results : The results showed that one cun obtained by the directional methods were significantly different from one cun by the proportional methods in forearm. It was demonstrated that the length acquired with the directional method was more correlated with body weight and body mass index, while the length obtained by the proportional method was more correlated with the height.
핵전이에 의한 Aspergillus oryzae var. oryzae와 Aspergillus nidulans 514의 잡종으로 부터 생산된 Xylanase의 특성
양영기,문명님,박형남,임채영 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1
Aspergillus oryzae var oryzae 와 Aspergillus nidulans 514간의 xylanase 우수 생성 종간 형질전환체를 획득하고, 유전분석과 정제를 통하여 다음과 같은 결과를 얻었다. 1. 핵전이를 위한 원형질체 형성 및 재생 조건에서는 Novozym 234의 농도 1%, 삼투안정제는 0.6M (NH_4)_2 SO_4, 효소의 처리 시간은 90분 그리고 최적 pH는 5.8로 나타났다. 2. 핵전이에 의한 형질전환의 빈도는 3×10 exp (-5)~1×10 exp (-5)으로 비교적 낮은 편이었다. 3. 유전적 안정성, conidia의 크기, DNA 함량의 측정 그리고 핵염색의 결과 형질전환체의 핵형은 aneuploid로 추정되었다. 4. 형질전환체의 xylanase 활성은 모균주와 비교하여 1.1~1.4배 증가하였다. 5. 형질전환체중 xylanase 활성이 가장 높은 TAVD514-3을 정제하고 몇가지 특성을 조사하였는데 전체적인 회수율이 17%, 분자량은 32,000 그리고 효소 활성에 미치는 기질의 영향을 올 조사하였다. 6. 정제된 효소의 최적 pH와 온도는 각각 pH 9.0과 80℃로 나타났으며, 효소활성은 Mg^2+ 와 Mn^2+의 존재시에 증가하는 경향을 나타내었다. Inerspecific hybrids between Aspergillus oryzae var oryzae and A. nidulans 514 were obtained by nuclear transfer technique. Several auxotrophic mutants isolated from conidiospores of the two strains were mutagenized with ultraviolet and N-methyl-N-nitrosoguanidine. Optimal conditions for formation of intergeneric hybrids were investigated. Frequencies of hybrid formation by nuclear transfer were 3×10 exp (-5)~1×10 exp (-5). From observation of genetic stability, conidial size, DNA content, and nuclear stain, it was suggested that their karyptypes are aneuploid. The hybrids showed 1.1~1.4 fold higher xylanase activities than parental strains did. The xylanase of Aspergiilus sp. TAVD514-3 was purified and some of it's enzymatc characteristics were investigated. The enzyme was purified about 85 fold with an overall yield of 17% from the culture medium by ammonium sulfate fractionation, Sephadex G-75 gel permeation chromatography, and CM-sephadex A-50 ion exchange chromatography. The purified enzyme functions optimally at pH 9.0 and 80℃. The enzymatic activity was increased by the presence of Mg^2+ and Mn^2 ions.
Bony cochlear nerve canal stenosis in pediatric unilateral sensorineural hearing loss
Lim, Chae-Hyun,Lim, Ji Hyung,Kim, Doyoun,Choi, Ho sung,Lee, Dong-Hee,Kim, Dong-Kee Elsevier 2018 INTERNATIONAL JOURNAL OF PEDIATRIC OTORHINOLARYNGO Vol.106 No.-
<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>This study was performed to evaluate the frequency of bony cochlear nerve canal (BCNC) stenosis and its clinical significance in pediatric patients with unilateral sensorineural hearing loss (SNHL) of unknown etiology.</P> <P><B>Materials and methods</B></P> <P>We analyzed the medical records and temporal bone computed tomography (CT) results of patients less than 13 years of age with a diagnosis of unilateral SNHL of unknown etiology between July 2007 and July 2017. We compared the BCNC diameter between both sides and analyzed the age at diagnosis, degree of hearing loss, and accompanying inner ear anomalies.</P> <P><B>Results</B></P> <P>In 42 patients, the mean age at diagnosis was 7.4 ± 3.6 years, and the average hearing level in the affected ear was 87.9 ± 20.0 dB HL (decibels hearing level). The average diameter of the BCNC was 1.22 ± 0.75 mm on the affected side and 1.96 ± 0.52 mm on the normal side. The most suitable criterion for BCNC stenosis appeared to be a diameter of 1.2 mm by the recursive partitioning procedure. With application of this criterion, the rate of BCNC stenosis was significantly greater on the affected side than on the normal side (52.4% vs. 4.8%, respectively; <I>P</I> < 0.05). A narrow internal acoustic canal was found in two patients, and vestibular and cochlear anomalies were found in three patients each.</P> <P><B>Conclusions</B></P> <P>Our results suggest that it is reasonable to set a diameter of 1.2 mm as a cutoff for BCNC stenosis, and also that BCNC stenosis is a common cause of unilateral SNHL of unknown etiology in childhood.</P>
Aquatide Activation of SIRT1 Reduces Cellular Senescence through a SIRT1-FOXO1-Autophagy Axis
Lim, Chae Jin,Lee, Yong-Moon,Kang, Seung Goo,Lim, Hyung W.,Shin, Kyong-Oh,Jeong, Se Kyoo,Huh, Yang Hoon,Choi, Suin,Kor, Myungho,Seo, Ho Seong,Park, Byeong Deog,Park, Keedon,Ahn, Jeong Keun,Uchida, Yos The Korean Society of Applied Pharmacology 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5
Ultraviolet (UV) irradiation is a relevant environment factor to induce cellular senescence and photoaging. Both autophagy- and silent information regulator T1 (SIRT1)-dependent pathways are critical cellular processes of not only maintaining normal cellular functions, but also protecting cellular senescence in skin exposed to UV irradiation. In the present studies, we investigated whether modulation of autophagy induction using a novel synthetic SIRT1 activator, heptasodium hexacarboxymethyl dipeptide-12 (named as Aquatide), suppresses the UVB irradiation-induced skin aging. Treatment with Aquatide directly activates SIRT1 and stimulates autophagy induction in cultured human dermal fibroblasts. Next, we found that Aquatide-mediated activation of SIRT1 increases autophagy induction via deacetylation of forkhead box class O (FOXO) 1. Finally, UVB irradiation-induced cellular senescence measured by $SA-{\beta}-gal$ staining was significantly decreased in cells treated with Aquatide in parallel to occurring SIRT1 activation-dependent autophagy. Together, Aquatide modulates autophagy through SIRT1 activation, contributing to suppression of skin aging caused by UV irradiation.
Aquatide Activation of SIRT1 Reduces Cellular Senescence through a SIRT1-FOXO1-Autophagy Axis
( Chae Jin Lim ),( Yong-moon Lee ),( Seung Goo Kang ),( Hyung W. Lim ),( Kyong-oh Shin ),( Se Kyoo Jeong ),( Yang Hoon Huh ),( Suin Choi ),( Myungho Kor ),( Ho Seong Seo ),( Byeong Deog Park ),( Keedo 한국응용약물학회 2017 Biomolecules & Therapeutics(구 응용약물학회지) Vol.25 No.5
Ultraviolet (UV) irradiation is a relevant environment factor to induce cellular senescence and photoaging. Both autophagy- and silent information regulator T1 (SIRT1)-dependent pathways are critical cellular processes of not only maintaining normal cellular functions, but also protecting cellular senescence in skin exposed to UV irradiation. In the present studies, we investigated whether modulation of autophagy induction using a novel synthetic SIRT1 activator, heptasodium hexacarboxymethyl dipeptide-12 (named as Aquatide), suppresses the UVB irradiation-induced skin aging. Treatment with Aquatide directly activates SIRT1 and stimulates autophagy induction in cultured human dermal fibroblasts. Next, we found that Aquatide-mediated activation of SIRT1 increases autophagy induction via deacetylation of forkhead box class O (FOXO) 1. Finally, UVB irradiation-induced cellular senescence measured by SA-β-gal staining was significantly decreased in cells treated with Aquatide in parallel to occurring SIRT1 activation-dependent autophagy. Together, Aquatide modulates autophagy through SIRT1 activation, contributing to suppression of skin aging caused by UV irradiation.
MKL-SVM 기반의 건전성 예측 및 관리기술(PHM)에 관한 연구
임채영(Chae-Young Lim),여채온(Chae-Eun Yeo),조우진(Woo-Jin Cho),이형아(Hyung-Ah Lee),구재희(Jae-Hoi Gu) 한국환경에너지공학회 2022 한국열환경공학회 학술대회지 Vol.2022 No.2
최근 폴랜트 산업에서는 IIoT(Industrial internet of things), AI(Artificial intelligence), 빅데이터 PHM(Prognostics and Health Management) 그리고 Digital Twin 등의 기술을 기반으로, 폴랜트 설비의 건전성상태를 실시간으로 모니터링하고 이로부터 고장여부 진단은 물론 고장까지 시간을 미리 예측하는 관리기술의 중요성이 강조되고 있다. 본 논문에서는 플랜트 설비(펌프, 압축공기, 보일러, 모터, 변압기)의 고장예지 및 사전 결함을 예측하는 건전성 예측 및 관리기술(Prognostics and health management, PHM) 의 고도화를 위해 기계학습 기반의 MKL-SVM 알고리즘을 제안한다. 제안된 MKL-SVM 알고리 즘과 CNN(Convolutional Neural Network)모델을 비교하여 성능평가를 하였으며, 제안된 방법으로 검증한 실험에서 사전 결함 예측 가능성이 지속적으로 개선되었음을 검증하였다. 또한, DeepLearning과 효용성 검증실험을 통해 제안하는 방법이 효율적임을 확인하였다.