천연 항균물질이 첨가된 소금의 식중독 세균에 대한 항균효과

현정은,박세은,이서현,이연진,장민경,문성권,이선영,Hyun, Jeong-Eun,Park, Se-Eun,Lee, Seo-Hyeon,Lee, Yeon-Jin,Jang, Min-Kyung,Moon, Sung-Kwon,Lee, Sun-Young 한국급식외식위생학회 2020 급식외식위생학회지 Vol.1 No.1

Salt is a common seasoning agent used in various processed foods, especially in kimchi and salted seafood (jeotgal). This study was conducted to investigate the efficacy of salt with antimicrobial substances (acetic acid, garlic extract, carvacrol, nisin, thymol, and their combination (acetic acid+nisin+thymol)) on improvement of antibacterial effects of salt against foodborne pathogens. Salt (10%) was prepared using six different types of 0.2% natural antimicrobial substances. The antibacterial effect of salt combined with natural antimicrobial substances was evaluated against foodborne pathogens using the broth micro-dilution method and growth curve plotted using absorbance measurements. For the five foodborne pathogens, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of salt without antimicrobial substances as control were in the range of 24~>50,000 ㎍/mL and >50,000 ㎍/mL, respectively. Salt with nisin, thymol, or garlic extract showed strong inhibitory effects and their MIC against L. monocytogenes were 49, 12,500, and 24 ㎍/mL, respectively. In particular, salt with nisin showed inhibitory activities against Gram-positive bacteria. However, all the antimicrobial substances were less effective against Gram-negative bacteria such as E. coli O157:H7 and S. Typhimurium than Gram-positive bacteria. These results could be used for the development of salt with natural antimicrobial substances especially targeted against L. monocytogenes. This would enable the lowering of saline concentration while improving the storability of food.

Antibacterial Activity of an Ethyl Acetate Extract of Pseudomonas sp. UJ-6 against Methicillin-Resistant Staphylococcus aureus

Lee, Dae-Sung,Eom, Sung-Hwan,Je, Jae-Young,Kim, Young-Mog,Lee, Myung-Suk,Kim, Young-Man The Korean Society of Fisheries and Aquatic Scienc 2013 Fisheries and Aquatic Sciences Vol.16 No.2

In an effort to discover an alternative antibiotic for treating infections with methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas sp. UJ-6, a marine bacterium that exhibited antibacterial activity against MRSA, was isolated. The culture broth and its ethyl acetate extract exhibited bactericidal activity against MRSA. The extract also exhibited antibacterial activity against gram-negative bacteria, which were not susceptible to vancomycin. The treatment of MRSA with the extract resulted in abnormal cell lysis. The extract retained >95% of its anti-MRSA activity after heat treatment for 15 min at $121^{\circ}C$. Thus, although most antibiotics are unstable under conditions of thermal stress, Pseudomonas sp. UJ-6 produces a heat-stable anti-MRSA substance. The results of this study strongly suggest that Pseudomonas sp. UJ-6 can be used to develop a novel, heat-stable, broad-spectrum antibiotic for the treatment of MRSA infections.

Antibacterial Activity of an Ethyl Acetate Extract of Pseudomonas sp. UJ-6 against Methicillin-Resistant Staphylococcus aureus

이대성,엄성환,김영목,이명숙,제재영,김영만 한국수산과학회 2013 Fisheries and Aquatic Sciences Vol.16 No.2

In an effort to discover an alternative antibiotic for treating infections with methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas sp. UJ-6, a marine bacterium that exhibited antibacterial activity against MRSA, was isolated. The culture broth and its ethyl acetate extract exhibited bactericidal activity against MRSA. The extract also exhibited antibacterial activity against gram-negative bacteria, which were not susceptible to vancomycin. The treatment of MRSA with the extract resulted in abnormal cell lysis. The extract retained >95% of its anti-MRSA activity after heat treatment for 15 min at 121°C. Thus, although most antibiotics are unstable under conditions of thermal stress, Pseudomonas sp. UJ-6 produces a heat-stable anti-MRSA substance. The results of this study strongly suggest that Pseudomonas sp. UJ-6 can be used to develop a novel, heat-stable, broad-spectrum antibiotic for the treatment of MRSA infections.

Original Article : Antibacterial Activity of an Ethyl Acetate Extract of Pseudomonas sp. UJ-6 against Methicillin-Resistant Staphylococcus aureus

( Dae Sung Lee ),( Sung Hwan Eom ),( Jae Young Je ),( Young Mog Kim ),( Myung Suk Lee ),( Young Man Kim ) 한국수산과학회(구 한국수산학회) 2013 Fisheries and Aquatic Sciences Vol.16 No.2

In an effort to discover an alternative antibiotic for treating infections with methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas sp. UJ-6, a marine bacterium that exhibited antibacterial activity against MRSA, was isolated. The culture broth and its ethyl acetate extract exhibited bactericidal activity against MRSA.The extract also exhibited antibacterial activity against gram-negative bacteria, which were not susceptible to vancomycin. The treatment of MRSA with the extract resulted in abnormal cell lysis. The extract retained<95% of its anti-MRSA activity after heat treatment for 15 min at 121°C. Thus, although most antibiotics are unstable under conditions of thermal stress, Pseudomonas sp. UJ-6 produces a heat-stable anti-MRSA substance. The results of this study strongly suggest that Pseudomonas sp. UJ-6 can be used to develop a novel, heat-stable, broad-spectrum antibiotic for the treatment of MRSA infections.

Antioxidant and antibacterial capabilities of phenolic compounds and organic acids from Camellia oleifera cake

Duoduo Zhang,Shaoping Nie,Mingyong Xie,Jielun Hu 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.1

There is growing interest in the antioxidants and antibacterial activity from natural substances. The purpose of the research was to gain and distinguish phenolic substances and organic acids in the Camellia oleifera cake, and to study their antioxidant and antibacterial activities. The extraction and purification of them were achieved by solvent extraction and column separation, respectively. The conclusions displayed that purity of the phenolic substances was 94.1 ± 0.5% w/w and that of organic acid was 96.0 ± 0.3% w/w; Fifteen phenolic substances were certificated using HPLC–ESI–MS technology; oxalic, citric, acetic, malic, and succinic acids are discovered to be main organic acids. In addition, the phenolic substances and organic acids both have good antioxidant activity and obvious inhibition against six species of bacteria. These conclusions can be useful in the reuse of the waste of Camellia oleifera oil industry in the future.

Diverse Antibacterial Activity of Pectobacterium carotovorum subsp. carotovorum Isolated in Korea

( Eun Jung Roh ),( Seung Don Lee ),( Yong Hoon Lee ),( Dong Su Ra ),( Jae Hyuk Choi ),( Eun Pyo Moon ),( Sung Gi Heu ) 한국미생물 · 생명공학회 2009 Journal of microbiology and biotechnology Vol.19 No.1

Fifty-four Pectobacterium carotovorum subsp. Carotovorum strains isolated in Korea were characterized by a spectrum of antibacterial activities against 7 indicator strains chosen to represent various regions and host plants. All P. carotovorum subsp. carotovorum isolates tested could be grouped into 4 classes depending on the pattern of antibacterial substance production. All tested strains had DNA fragment(s) homologous to the genes encoding carotovoricin and 21 of them had genes homologous to DNA invertase. Sixteen strains had genes homologous to the genes encoding carocin S1. Several isolates produced antibacterial substances active against strains in Brenneria, Pantoea, and Pectobacterium genera that belonged formerly to the genus Erwinia. Strains in Pseudomonas or Xanthomonas sp. were not sensitive to the antibacterial substances produced by P. carotovorum subsp. carotovorum, except for X. albilineans that was sensitive to antibacterial substances produced by most strains in P. carotovorum subsp. carotovorum and P. betavasculorum KACC10056. These results demonstrated the diverse patterns of antibacterial substance production and the possibility of the existence of new antibacterial substance(s) produced by P. carotovorum subsp. carotovorum isolated in Korea.

Purification and Characterization of an Antibacterial Substance from Aerococcus urinaeequi Strain HS36

성호선,조윤래 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.1

A bacterial strain inhibiting the growth of Vibrio anguillarum, the causative agent of vibriosis, was isolated from fish intestines. The isolated strain HS36 was identified as Aerococcus urinaeequi based on the characteristics of the genus according to Bergey’s Manual of Systematic Bacteriology and by 16S rRNA sequencing. The growth rate and antibacterial activity of strain HS36 in shaking culture were higher than those in static culture, while the optimal pH and temperature for antibacterial activity were 7.0 and 30°C, respectively. The active antibacterial substance was purified from a culture broth of A. urinaeequi HS36 by Sephadex G-75 gel chromatography, Sephadex G-25 gel chromatography, and reverse-phase high-performance liquid chromatography. Its molecular weight, as estimated by Tricine SDS-polyacrylamide gel electrophoresis, was approximately 1,000 Da. The antibacterial substance produced by strain HS36 was stable after incubation for 1 h at 100°C. Although its antibacterial activity was optimal at pH 6-8, activity was retained at a pH range from 2 to 11. The purified antibacterial substance was inactivated by proteinase K, papain, and β-amylase treatment. The newly purified antibacterial substance, classified as a class II bacteriocin, inhibited the growth of Klebsiella pneumoniae, Salmonella enterica, and Vibrio alginolyticus.

Antibacterial Effect of Antibacterial Substance Produced by Lactobacillus amylovorus IMC-1 against Food Spoilage Bacteria

Mok, Jong-Soo,Kim, Poong-Ho,Yu, Hyen-Duk,Kim, Ji-Hoe,Lee, Hee-Jung,Kim, Young-Mog 한국식품위생안전성학회 1999 한국식품위생안전성학회지 Vol.14 No.4

본 연구에서는 발효 식품의 제조에 유용한 항균성 물질을 생산하는 유산균 starter의 개발의 일환으로 장내 상존균으로 알려진 Lactobacillus acidophilus group유산균인 Lactobacillus amylovorus IMC-1 균주가 생산한 항균성 물질의 식품오염세균에 대한 항균 활성을 검토하였다. 내몽골 원산 치즈에서 분리된 L. amylovorus IMC-1 균주는 탈지유배지에서 37$^{\circ}C$에서 72시간 배양하였을 때 최대로 항균성 물질을 생산하였으며, 더 이상의 배양은 항균성 물질의 생산에 영향을 미치지 않았다. 겔 여과후의 항균성 물질은 식품오염세균인 Bacillus subtilis IFO 3025, staphylococcus aureus IAM 1011, Listeria monocytogenes VTU 206, Escherichia coli RB. 및 Pseudomonas fragi IFO 3458등과 같은 모든 피검균에 대하여 20units/ml 첨가로 항균활성을 나타내었다. 그리고 이 물질은 B. subtilis, E. coli및 Ps. fragi에 대해서는 살균 작용을 나타내었으며, Staphy. aureus와 L. monocytogenes에 대해서는 정균 작용을 보였다. 그 살균 작용은 용균 작용에 기인한 것임이 밝혀졌다. 또한 이 물질은 유기산, 과산화수소 및 단백질성 물질이 아님이 밝혀졌다. To develop a lactic starter to produce antimicrobial substance for inhibiting the growth of a variety of foodborne spoilage bacteria in fermented foods, we investigated the anti-bacterial effect of the antibacterial substance, produced by Lactobacillus amylovorus IMC-1, against foodborne spoilage strains, and its sensitivity on the treatment of proteolytic enzymes. L. amylovorus IMC-1, which was isolated from a traditional cheese in Inner Mongolia, produced a maximum amount of antibacterial substance in the skim milk medium after 72 h incubation at 37$^{\circ}C$, and further incubation resulted in the same activity. The substance obtained from gel filtration inhibited all strains used such as Bacillus subtilis IFO 3025, Staphylococcus aureus IAM 1011, Listeria monocytogenes VTU 206, Escherichia coli RB, and Pseudomonas fragi IFO 3458 at the concentration of 20 units/ml. This substance was found to show bactericidal action against B. subtilis, E. coli, and Ps. fragi, and bacteriostatic activity against both Staph. aureus and L. monocytogenes. The bactericidal action was due to cellular Iysis. The substance is not organic acid, hydrogen peroxide and proteinaceous compound.

여드름을 유발하는 Propionibacterium acnes에 대한 분리 세균들의 항균활성

이다솔,송홍규,Lee, Da-Sol,Song, Hong-Gyu 한국미생물학회 2018 미생물학회지 Vol.54 No.3

본 연구는 여드름을 유발하는 세균인 Propionibacterium acnes에 대해 다양한 토양에서 분리된 세균 균주들의 항균효과를 조사하기 위해 수행되었다. 수백 개의 분리 세균균주 중 Paenibacillus elgii DS381과 Paenibacillus elgii DS1515, Burkhoderia gladioli DS518, Streptomyces lienomycini DS620는 2가지 균주의 P. acnes에 대해 높은 항균활성을 나타내었다. 이 분리균주들은 agar well diffusion test에서 15.5~34.3 mm 직경의 저해대를 형성하였으며, 특히 DS620는 가장 큰 저해대 직경(28.3~34.3 mm)을 나타내었다. 분리 균주가 생성하는 항균 물질은 DS381과 DS1515 균주의 경우lipopeptide (pelgipeptin, paenipeptin), DS518은 protease, 그리고 DS620은 anthracycline 인 것으로 추정되며, 이들 모두 P. acnes에 대해매우낮은 최소저해농도를 나타내었다[DS381와 DS1515 (0.078 mg/ml), DS518 (0.312 mg/ml), DS620 (0.000078 mg/ml)]. P. acnes를 대상으로 한 time-kill assay에서는 네 균주의 항균물질이 모두 24시간 이내에 P. acnes를 완전히 사멸시켰다. 이 결과는 네 가지 항균활성 균주들이 분비하는 항균물질들이 여드름을 유발하는 P. acnes에 대하여 효율적인 치료 소재로 사용될 수 있는 가능성을 보여준다. This study was carried out to evaluate antimicrobial activity of isolated bacteria from various soils against two strains of Propionibacterium acnes causing acne vulgaris. Among several hundreds of bacterial strains, Paenibacillus elgii DS381, Paenibacillus elgii DS1515, Burkholderia gladioli DS518, and Streptomyces lienomycini DS620 showed high antimicrobial activities against the strains of P. acnes. All isolated bacteria showed 15.5 to 34.3 mm inhibition zone diameter in an agar well diffusion test, and especially DS620 showed the highest inhibition zone diameters (28.3~34.3 mm). Antibacterial substances were expected as lipopeptide (pelgipeptin and paenipeptin) from strains DS381 and DS1515, protease from DS518, and anthracycline antibiotic (daunomycinone) from DS620, and all these showed very low minimum inhibitory concentration [DS381 and DS1515 (0.078 mg/ml), DS518 (0.312 mg/ml), DS620 (0.000078 mg/ml)] against P. acnes. These antibacterial substances could completely kill P. acnes within 24 h in a time-kill assay. These results suggest that antibacterial substances produced by these bacteria may be utilized as useful antimicrobial agent against P. acnes and treatment medicine for acne vulgaris.

토양으로부터 항세균성 물질을 생산하는 Streptomyces sp. 의 분리

오현정,정완석,김세재,김창진,고영환 제주대학교 생명과학연구소 1999 제주생명과학연구 Vol.2 No.-

제주도의 토양에서 분리된 방선균 307주를 대상으로, paper disc법과 시험관 희석 배양법을 사용하여 항세균성 물질을 생산하는 균주를 선별하였다. 약 3.3%에 상당하는 분리주들이 Escherichia coli 8749, Staphylococcus aureus 6538 그리고 Pseudomonas solanacearum 10692에 대해 항균효과를 나타냈고, 그 중에서 가장 높은 항균활성을 나타내는 BL93 균주를 최종선별하여 동정하였다. BL93 균주의 배양학적, 형태학적, 생리학적, 생화학적 그리고 화학분류학적 특성을 기준으로 볼 때 Streptomyces violaceus와 유사하였기에, Streptomyces sp. BL93으로 명명하였다. 이 균주가 생산하는 항세균성 물질은 butanol보다도 물에 더 잘 녹는 극성화합물로 chloroform이나 ethylacetate에는 거의 용해되지 않았다. 100℃에서 30분간 가열 처리해도 활성의 소실이 거의 없었고, pH 3∼9범위에서 안정하였다. 분리균주 BL93은 aspartocin을 생산하는 Streptomyces violaceus와 유사함으로, BL93이 생산하는 항세균성 물질의 동정 및 aspartocin과의 비교 검토가 선행되어야 할 것이다. Three hundred and seven isolates of Actinomycetes were isolated and purified from soil samples in Cheju Is. to screen antibacterial compounds. About 3.3% of the isolates showed antibacterial activity, and one of them, BL93, showed relatively higher antibacterial activity against Escherichia coli 8749, Staphylococcus aureus 6538 and Pseudomonas solanacearum 10692 by both paper disc method and tube dilution incubation method. The isolate BL93 was similar to Streptomyces violaceus based upon its cultural, morphological, physiological, biochemical and chemotaxanomic characteristics. It was designated as Streptomyces sp. BL93. The antibacterial substance produced by the isolate BL93 had higher affinity for water than butanol and was insoluble in chloroform or ethylacetate. Most of its antibacterial activity was retained even after heating at 100℃ for 30min and was stable at pH ranges 3 to 9. As the isolate BL93 was tentatively identified to be Streptomyces violaceus, the antibacterial substance preferentially need to be identified and compared with aspartocin, an antimicrobial compound produced by Streptomyces violaceus.