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        • KCI등재

          GPD 프로모터를 이용한 항균활성 효모의 활성증진

          장민경,유기환,김남영,이옥희,신재균,장혜지,이승우,이동근,이상현,Jang, Min-Kyung,Yu, Ki-Hwan,Kim, Nam-Young,Lee, Ok-Hee,Shin, Jae-Kyun,Jang, Hye-Ji,Lee, Seung-Woo,Lee, Dong-Geun,Lee, Sang-Hyeon 한국생명과학회 2010 생명과학회지 Vol.20 No.6

          박테리오신의 항균활성 개선을 위해 선행연구에서 개발된 ADH 프로모터에 의해 박테리오신 유전자를 발현시키는 효모발현 벡터에 GPD 프로모터 단편을 도입하여 강력한 프로모터를 가진 효모발현 재조합 플라스미드DNA를 제작하였다. ADH 프로모터에 의해 박테리오신 유전자를 발현시키는 기존의 형질전환 효모들에 비해 새롭게 개발된 GPD 프로모터에 의해 박테리오신 유전자를 발현시키는 형질전환 효모들이 그람양성 대표세균인 고초균(B. subtilis)과 그람음성 장내세균인 대장균(E. coli) 모두에서 보다 높은 생육억제환을 나타내는 것을 확인 하였다. 이 연구의 결과로 GPD 프로모터에 의해 발현이 유도되는 박테리오신 생산 효모들을 이용하여 부패하기 쉬운 식품들의 보존성을 향상시키기 위한 보존제 대체물질 또는 가축 사료에서 병원균의 생육을 저해하기 위한 항생제 대체물질의 산업적인 생산이 가능하게 될 것으로 기대된다. We have previously reported recombinant productions of bacteriocins using yeast expression plasmid pAUR123, which contains the alcohol dehydrogenase (ADH) promoter, in Saccharomyces cerevisiae cells and their antibacterial activities. In order to improve the antibacterial activities of bacteriocidal yeast cells, a strong glyceraldehyde phosphate dehydrogenase (GPD) promoter gene of S. cerevisiae was amplified and inserted upstream into bacteriocin genes such as the OR-7, Subpeptin JM4-A or JM4-B gene in the corresponding recombinant yeast plasmid. Yeast cells transformed by the recombinant plasmid containing the GPD promoter represented higher antibacterial activities against both Gram positive B. subtilis and Gram negative E. coli cells compared to those transformed by the corresponding recombinant plasmid containing the ADH promoter. Thus, yeast cells harboring the recombinant plasmid containing the GPD promoter constructed in this study could be applied in the food preservative or animal feed industries.

        • KCI등재

          전북 완주 신풍유적 출토 초기철기시대 동검의 부식 및 제작특성

          장민경,조남철 대한금속·재료학회 2020 대한금속·재료학회지 Vol.58 No.7

          The subject of this study was a bronze sword excavated from the early Iron Age area of Sinpung site in Wanju. SEM-EDS, FE-EPMA and Raman Micro-Spectroscopy analysis methods were applied to investigate the manufacturing techniques and corrosion characteristics. The results of the analysis indicated a casting structure composed of both α and α+δ phases. The α phase developed in the form of dendrite, and it seems that no heat treatment was performed. Through the EDS analysis results, the main components of the bronze sword were identified as Cu, Sn, Pb, etc., and it seems that the cooling rate was different depending on the shape of the dendrite, which is the α phase, and the size and distribution of the Pb particles. Regarding corrosion characteristics, the corrosion progressed considerably to the surface, and a high tin corrosion layer was formed, and lead oxide and copper oxide particles were observed between the corroded microstructures. These materials were identified by Raman spectroscopic analysis as Cuprite (Cu2O), Malachite (Cu2CO3(OH)2), Cassiterite (SnO2), Cerussite (PbCO3), Anglesite (PbSO4), and Litharge (PbO). Among them, the presence of nanocrystalline SnO2 confirmed the characteristics of long-term corrosion of bronze artifacts in the burial environment. And, materials such as Cerussite (PbCO3), Anglesite (PbSO4), and Litharge (PbO) were found only in weapons and tools such as bronze sword and spear when compared with previous studies.

        • KCI등재

          GD-MS 분석 장비를 활용한 극미량 무기물 질량 분석을 위한 표준RSF 구축 및 응용

          장민경,양재열,이종현,윤재식 한국분석과학회 2018 분석과학 Vol.31 No.6

          The present study analyzed standard samples of three types of aluminum matrix certified reference materials (CRM) using GD-MS. Calibration curves were constructed for 13 elements (Mg, Si, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, Ga, Sn, and Pb), with the slope representing the relative sensitivity factor (RSF). The x- and y-axes of the calibration curve represented ion beam ratio (IBR) and the authenticated value of the standard sample, respectively. In order to evaluate precision and linearity of the calibration curve, RSD and the coefficient of determination were calculated. Curve RSD for every element reflected high precision (within 10 %). For most elements, the coefficient of determination was ≥ 0.99, indicating excellent linearity. However, vanadium, nickel, and gallium curves exhibited relatively low linearity (0.90~0.95), likely due to their narrow concentration ranges. Standard RSF was calculated using the slope of the curve generated for three types of CRM. Despite vanadium, nickel, and gallium exhibiting low coefficients of determination, their standard RSF resembled that of the three types of CRM. Therefore, the RSF method may be used for element quantitation. Standard iron matrix samples were analyzed to verify the applicability of the aluminum matrix standard RSF, as well as to calculate the RSD-estimated error of the measured value relative to the actual standard value. Six elements (Al, Si, V, Cr, Mn, and Ni) exhibited an RSD of approximately 30 %, while the RSD of Cu was 77 %. In general, Cu isotopes are subject to interference: 63Cu to 54Fe2+-36Ar and 65Cu to 56Fe-Al3+ interference. Thus, the influence of these impurities may have contributed to the high RSD value observed for Cu. To reliably identify copper, the resolution should be set at ≥8000. However, high resolutions are inappropriate for analyzing trace elements, as it lowers ion permeability. In conclusion, quantitation of even relatively low amounts of six elements (Al, Si, V, Cr, Mn, and Ni) is possible using this method. 본 연구에서는 GD-MS를 활용하여 원소 별 함량이 다른 세 종류의 알루미늄 매질의 표준 시료를 분석하였다. 13종의 원소(Mg, Si, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, Ga, Sn, Pb)에 대해 검량 곡선을 작성하고 그 기울기를 RSF(Relative sensitivity reference)로 확립하였다. 검량 곡선은 X축을 IBR(Ion beam ratio)로, Y축을 표준 시료의 인증 값으로 작성하였다. 검량 곡선의 정밀도와 직선성을 평가하기 위해RSD(Relative standard deviation)와 결정 계수를 계산하였다. 그 결과 모든 원소의 RSD는 10%이내로 높은 정밀도를 나타냈다. 바나듐, 니켈 그리고 갈륨 원소를 제외한 대부분의 원소들은 결정 계수가 0.99 이상으로 1에 가까운 값을 얻어 직선성이 우수했다. 바나듐, 니켈 그리고 갈륨 원소는 결정 계수가 0.90~0.95 범위로 비교적 낮은 직선성을 나타냈으며, 이는 좁은 농도 범위로 인한 오차로 판단된다. 바나듐, 니켈그리고 갈륨 원소는 결정 계수는 낮지만 각각의 표준 시료 RSF와 기울기로 확립한 표준RSF(Standard- RSF)가 비슷하여 정량 분석을 위한 RSF로 활용 가능할 것으로 판단된다. 다른 매질의 시료에 표준RSF(Al matrix)를 적용 가능 여부와 실제 표준 값에 대한 오차를 확인하기 위해서 철 매질(Fe matrix)의 표준 시료를 분석하여 검증하였다. 구리 원소를 제외한 6종(Al, Si, V, Cr, Mn, Ni) 원소의 오차율은 약 30%로나타났으며, 구리 원소는 측정을 방해하는 불순물 화합물의 영향으로 오차율이 크게 나타난 것으로 판단된다. 일반적으로 동위원소63Cu는 54Fe2+-36Ar 간섭을 받고 65Cu는 56Fe-Al3+간섭을 받는다. 이를 분해하기위해서는 8000 이상의 분해능이 필요하다. 하지만, 높은 분해능은 이온의 투과도를 낮추기 때문에 미량원소 분석에 어려움이 있다. 구리 원소를 제외한 알루미늄 외 5종의 원소에 대해서는 비교적 적은 오차로 정량 분석이 가능한 것으로 확인되었다.

        • KCI등재

          이동로봇의 안전한 엘리베이터 탑승을 위한 RGB-D 센서 기반의 엘리베이터 인식 및 위치추정

          장민경,조현준,송재복 한국로봇학회 2020 로봇학회 논문지 Vol.15 No.1

          Multi-floor navigation of a mobile robot requires a technology that allows the robot to safely get on and off the elevator. Therefore, in this study, we propose a method of recognizing the elevator from the current position of the robot and estimating the location of the elevator locally so that the robot can safely get on the elevator regardless of the accumulated position error during autonomous navigation. The proposed method uses a deep learning-based image classifier to identify the elevator from the image information obtained from the RGB-D sensor and extract the boundary points between the elevator and the surrounding wall from the point cloud. This enables the robot to estimate the reliable position in real time and boarding direction for general elevators. Various experiments exhibit the effectiveness and accuracy of the proposed method.

        • KCI등재

          The Efficacy of Language Learning Strategy Instruction in a Korean High School English Class

          장민경 한국중등영어교육학회 2019 중등영어교육 Vol.12 No.3

          This paper examines the efficacy of language learning strategy instruction based on the cognitive academic language learning approach (CALLA). This paper studies the effect of language learning strategy instruction with particular reference to English proficiency, learning strategies, and the relationship between such strategies and motivation of a Korean high school student. In this study, seventy-nine high school students were given twenty weeks of instruction. The control groups were given lectures without employing a language learning strategy programs; conversely, the experimental groups utilized language learning strategies. In order to assess student language proficiency, pre- and post-tests were given. SILL and questionnaires related to motivation as well as language learning strategy use were also adopted. Results showed that the scores of less-proficient students in the experimental group dramatically improved. The most frequently used strategy was cognitive strategy, while metacognitive strategy was reported least applied. After CALLA instruction, the experimental group of students used metacognitive strategy more actively. In addition, there was a strong correlation between language proficiency and language learning strategy use. This study also indicates that learner motivation influenced language learning strategies. Therefore, the implementation of language learning strategy instruction based on the cognitive academic language learning approach (CALLA) should be considered in language classes in Korean high schools.

        • KCI등재

          갈파래(Ulva lactuca) 추출분획의 암 세포주에 대한 세포독성 및 면역활성 효과

          장민경,김남영,이동근,이재화,하종명,하배진,김미향,배송자,장정수,이상현,Jang, Min-Kyung,Kim, Nam-Young,Lee, Dong-Geun,Lee, Jae-Hwa,Ha, Jong-Myung,Ha, Bae-Jin,Kim, Mi-Hyang,Bae, Song-Ja,Jang, Jeong-Su,Lee, Sang-Hyeon 한국생명과학회 2006 생명과학회지 Vol.16 No.7

          본 연구는 갈파래(Ulva lactuca) 추출분획의 암세포주에 대한 세포독성 및 면역활성 효과를 조사하였다. 갈파래의 에탄올 추출물로부터 분획물의 제조는 hexane, ethyl ether, methanol, butanol, $H_2O$의 용매를 이용하여 행하였다. 인간 백혈암세포주(U937), 생쥐 신경아종세포주(NB41A3), 인체간암세포주(HepG2),큰지 신경교세포주(C6) 등에 대한 갈파래 분획물의 세포독성을 측정하였다. 갈파래의 Ethyl ether 층은 4종류의 세포 모두에서 가장 높은 세포독성을 나타냈다. 또한 수층 역시 비교적 높은 세포독성을 나타냈다. 4종류의 세포 모두에서 농도의존적 경향을 나타냈다. 갈파래 분획물의 큰쥐 대식세포주(RAW 264.7)에 대한 면역활성 효과도 조사하였다. 갈파래 추출물의 5가지 분획물 모두에서 농도의존적으로 NO 생산을 활성화시켰다. 이러한 결과들로 잔파래가 항암 덴 면역활성을 나타내는 천연 소재개발에 있어 유용한 후보가 될 수 있을 것으로 사료된다. Extracted fractions of the green seaweed Ulva lactuca were studied to verify the cytotoxicity and immunostimulating activity. The fractions from the ethanol extract of U. lactuca were prepared by the systematic extraction procedure with solvents such as hexane, ethyl ether, methanol, butanol and $H_2O$. The cytotoxic effects of U. lactuca fractions against human leukemia cell line U937, mouse neuroblastoma cell line (NB41A3), human hepatoma cell line (HepG2) and rat glioma cell line (C6) were investigated. Ethyl ether fraction showed the highest cytotoxicity against all four cell lines tested. In addition, $H_2O$ fraction also showed relatively high cytotoxicity. Dose dependent patterns were observed on all four cell lines. The immune-stimulating effects of U. lactuca fractions on rat macrophage cell line (RAW 264.7) were also investigated. All five fractions of U. lactuca extract stimulated NO production with concentration dependant manner. These results suggest that U. lactuca may be a useful candidate for a natural cancer preventing and immune-stimulating agents.

        • KCI등재

          Template-assisted Morphological Evolution of Cu2S and Cu2MoS4 from Octahedral Cu2O Crystals

          장민경,조영식,박가람,오인환,허영덕 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.11

          Various morphologies of Cu2S and Cu2MoS4 were synthesized from an octahedral Cu2O template in the presence of various concentrations of sodium molybdate and thioacetamide by carrying out solvothermal reactions in an ethylene glycol solvent. The morphology varied from hollow octahedral Cu2S structures, through small pieces of P-Cu2MoS4, and finally to thin square-like plates of I-Cu2MoS4, depending on the concentrations of sodium molybdate and thioacetamide. The XRD data indicated that the Cu2MoS4 structural phases changed from P2m to I2m the concentrations of sodium molybdate and thioacetamide increased.

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