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Photobacterium damselae의 디스크 확산법 결과에 대한 Epidemiological Cut-off Value의 결정
권문경 ( Mun-gyeong Kwon ),임윤진 ( Yun-jin Lim ),김명석 ( Myoung-sug Kim ),서정수 ( Jung-soo Seo ),김도형 ( Do-hyung Kim ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.6
In this study, epidemiological cut-off values were estimated for 44 Photobacterium damselae isolates, since clinical breakpoints have not been established for this pathogenic bacterium. The susceptibility of the isolates to 10 antibiotics was evaluated using internationally standardized disc diffusion protocols. Normalized resistance interpretation was used to generate statistically valid epidemiological cut-off values for the susceptibility data. There were not enough strains exhibiting full sensitivity to ampicillin and amoxicillin to allow analysis of these antibiotics. Because there were only a marginally sufficient number of strains exhibiting full sensitivity to oxytetracycline, the cut-off value generated provided only a provisional estimate. The valid wild-type cut-off values were <13, 13, 9, 22, 25, 27, and 28 mm for gentamicin, cephalexin, erythromycin, oxolinic acid, flumequine, florfenicol, and sulfamethoxazole/trimethoprim, respectively. The application of these cut-off values should greatly facilitate the rational selection of antibiotics for use in commercial fish farms.
어류 부레의 형태학적 차이에 따른 음향산란강도의 자세 및 주파수 의존성의 변화
이대재 ( Dae Jae Lee ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.2
Controlled broadband acoustic scattering laboratory experiments were conducted using a linear chirp signal (95-220 kHz), and x-ray images of live and model fish with an artificial swim bladder were analyzed to investigate the changesin orientation and frequency dependence of target strength (TS) due to morphological differences in fish swim bladders. The broadband echoes from live and model fish were measured over an orientation angle range of ± 45° in the dorsal plane and in approximately 1° increments. The location of nulls in the simulated echo response of the SINC [sin c function] model was overlaid on the TS map, showing the orientation and frequency dependence of fish TS, andthey matched very well. It was possible to infer the equivalent fish scattering size (or swim bladder) using the null spacing in the experimentally obtained broadband TS map. Good agreement was observed for inferring the equivalent scattering size between the SINC model and the broadband echoes measured for the three fish species (black scraper Thamnaconus modestus; goldeye rockfish Sebastes thompsoni; and whitesaddled reef fish Chromis notatus). Some results of this inference are discussed.
양식 넙치(Paralichthys olivaceus)에 경구투여한 Ampicillin 및 Amoxicillin의 어류체내 잔류량의 변화
손광태 ( Kwang Tae Son ),조미라 ( Mi Ra Jo ),오은경 ( Eun Gyoung Oh ),목종수 ( Jong Soo Mok ),권지영 ( Ji Young Kwon ),이태식 ( Tae Seek Lee ),송기철 ( Ki Cheol Song ),김풍호 ( Poong Ho Kim ),이희정 ( Hee Jung Lee ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.5
Residues of ampicillin and amoxicillin in the muscles of olive flounder Paralichthys olivaceus were investigated after oral doses of 20 mg/kg body weight/day of ampicillin and 40 mg/kg body weight/day of amoxicillin in a first examination and 40 mg/kg body weight/day of ampicillin and 80 mg/kg body weight/day of amoxicillin in a second examination for 5-6 days. The ranges of seawater temperatures in the 1st and 2nd examinations were 13.4-15.6oC and 16.8-21.4oC, respectively. Ampicillin and amoxicillin concentrations were analyzed by high performance liquid chromatography (HPLC). The average recovery rates of ampicillin and amoxicillin in fish samples ranged from 84.8- 95.2% and 100.8-103.8%, respectively. Residual concentrations in the olive flounder peaked on the 5th day, with average concentrations of 0.075 mg/kg and 0.311 mg/kg in the 1st examination, and 0.098 mg/kg and 0.630 mg/kg in the 2nd examination for ampicillin and amoxicillin, respectively, with maximum concentrations in muscle. In the 1st examination, ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentrations after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. In the 2nd examination, the ampicillin concentrations in olive flounder after withdrawals of 1 and 3 days were 0.041 mg/kg and 0.023 mg/kg, respectively, and amoxicillin concentration after withdrawals of 1 day and 10 days were 0.172 mg/kg and 0.023 mg/kg, respectively. We suggest that the recommended withdrawal periods should be 3 days for ampicillin and 10 days for amoxicillin in the olive flounder.
이두석 ( Doo Seog Lee ),윤호동 ( Ho Dong Yoon ),김연계 ( Yeon Kye Kim ),윤나영 ( Na Young Yoon ),문수경 ( Soo Kyung Moon ),김인수 ( In Soo Kim ),정보영 ( Bo Young Jeong ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
Proximate and fatty acid compositions in muscle tissue were studied in 14 species of coastal and offshore fishes in Korea. Lipid content ranged from 0.57 to 10.5% and was higher in migratory and reef dwelling fishes than in demersal fishes. Protein content ranged from 14.4 to 20.3% and moisture content ranged from 72.4 to 81.8% in all fish samples. There was a negative correlation between lipid and moisture content in all fish samples (r= -0.90, P<0.001). The prominent fatty acids were 16:0, 22:6n-3 (docosahexaenoic acid, DHA), 18:1n-9, 20:5n-3 (eicosapentaenoic acid, EPA), 16:1n-7, 18:0 and 18:1n-7. Most demersal fishes contained considerably higher amounts of 20:4n-6 and/or 22:5n-3 compared with migratory and reef dwelling fishes. The proportion of total polyunsaturated fatty acid (PUFA), including DHA and EPA, was higher in demersal fishes (38.6-54.0%) than in migratory and reef dwelling fishes (23.5-35.2%).
어획특성을 이용한 삼치(Scomberomorus niphonius)의 회유경로 추정
김희용 ( Heeyong Kim ),임유나 ( Yu Na Lim ),송세현 ( Se Hyun Song ),김영혜 ( Yeong Hye Kim ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.3
Seasonal catch distributions of large purse seines and daily landings of coastal set nets were analyzed to understand the migration path of the Spanish mackerel Scomberomorus niphonius around Korean waters. The Spanish mackerel start to move toward the coastal region in the South and West Sea in May for spawning and stay until July, when spawning finishes. Afterwards, they start to migrate to the East China Sea and the southern East Sea and are found irrespective of the onshore and offshore regions. Therefore, they disappear from the offshore region during the spawning season in June and July, and a new recruitment population of age 0 begins to inflow into the coastal fishing grounds in the South Sea and West Sea in August, one month earlier than in the Japanese coastal region of the East Sea.
해양심층수로 배양한 해양미세조류 Tetraselmis sp. JK-46의 성분 조성 및 생리활성
주동식 ( Dong Sik Joo ),김광우 ( Kwang Woo Kim ),조순영 ( Soon Yeong Cho ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.1
This study examined Tetraselmis sp. JK-46 isolated from seawater from the East Sea. Deep seawater (OSW) had a greater effect on the growth of Tetraselmis sp. JK-46 than surface seawater (SSW). The crude protein, lipid, carbohydrate and ash contents of Tetraselmis sp. JK-46 cultured with DSW were 27.2, 37.1, 13.2 and 26.3 %, respectively, and these values were similar to the results for samples cultured with SSW. The contents of Mg, Ca, Fe and K in the DSW cultured samples were 7080.3, 1009.6,251.2, and 2749.7 mg/IOO g, respectively. The fatty acid compositions of Tetraselmis sp. JK-46 cultured with DSW and SSW were 53.7 and 49.0 % polyunsaturated fatty acids (PUFA) and 25.7 and 30.7% saturated fatty acids (SF A), respectively. The total amino acid contents of the samples cultured with DSW and SSW were 7392.6 and 6376.0 mg/100 g respectively. The 1,l-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity of Tetraselmis sp. JK-46 extracts increased with the concentration of the chloroform and ethyl acetate fractions. The half maximal inhibitiory concentrations (IC50) of the chloroform and ethyl acetate fractions of OS W and SSW cultured samples were 1.2 and 2.6 mg/mL, and 3.1 and 3.3 mg/mL, respectively. The ethyl acetate fractions of DSW and SSW cultured samples has anticoagulant activity and the activated partial thromboplastin times (APTT) were 93.4 and 89.3 sec., respectively. The chloroform and ethyl acetate fractions showed antimicrobial activity against Bacillus subtilis, Escherichia coli and Candida albicans.
낙동강 하구에 이식된 잘피(Zostera marina)의 환경변화에 따른 성장특성
박정임 ( Jung Im Park ),이근섭 ( Kun Seop Lee ),손민호 ( Min Ho Son ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.5
Numerous seagrass habitat restoration projects have been attempted recently due to the remarkable decline in seagrass coverage. Seagrass transplants tend to adapt to a new environment after experiencing transplanting stress during the early stages of transplantation. Once acclimated, the transplants grow into healthy seagrass beds via vegetative propagation. The establishment and growth dynamics of transplanted seagrasses in bays and coasts are widely reported, but few studies have been conducted on estuaries in Korea. We transplanted Zostera marina in November 2007 and November 2008 in the Nakdong estuary using the staple method, and monitored the survival, adaptation, and growth dynamics of the transplants as well as environmental factors every month for 1 year. Both transplants adapted well to the new environment without initial losses and showed rapid productivity during early summer. However, density of transplants increased 320% in 1 year from the previous year`s transplants but that decreased to 59% during the following year. This significant reduction in density in the second year may have been caused by exposure to low salinity (10 psu) for 3 weeks during the unusually long monsoon season. While the survival and growth dynamics of seagrass transplants planted in bays and coasts are mainly controlled by underwater photon flux density and water temperature, salinity was the critical factor for those planted in Nakdong estuary.
어류 중 4계열 잔류 항생물질 검출을 위한 Lateral Flow Immunoassay Kit 개발
조미라 ( Mi Ra Jo ),손광태 ( Kwang Tae Son ),권지영 ( Ji Young Kwon ),목종수 ( Jong Soo Mok ),박홍제 ( Hong Jae Park ),김현용 ( Hyun Yong Kim ),김경동 ( Gyung Dong Kim ),김지회 ( Ji Hoe Kim ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2015 한국수산과학회지 Vol.48 No.2
A lateral flow immunoassay kit based on antigen-antibody interactions was developed to detect residues of beta-lactams, quinolones, tetracyclines, and sulfonamides in farmed fish. Group-specific antibodies showing cross-reactivity with other antibiotics in the same group were produced in rabbits. The rabbits were immunized eight times to obtain the maximum titers. Antibodies were extracted from the antisera collected from the immunized rabbits and produced group-specific reactions with antibiotics from the four groups. A kit was prepared that optimize conditions for the antigen-antibody reaction, using colloidal gold conjugated antibodies, and was designed to detect the four groups of antibiotics simultaneously. The kit enabled the detection of antibiotics in the four groups at below maximum residue limits (MRLs), which were 200 μg/kg for tetracyclines, 100 μg/kg for sulfonamides, 50 μg/kg for beta-lactams, and 100 μg/kg for quinolones. The cross-reactivity of the antibodies ranged from 10-80% for the sulfonamides, 20- 100% for tetracyclines, 38-100% for quinolones, and 20-100% for the beta-lactams, confirming that the antibodies were group specific. The test kit was used 30 times to examine spiked antibiotics at the limits of detection (LODs) and all produced positive results, indicating high sensitivity. The LODs for the assay ranged from 4-20 ng/mL for beta-lactams, 25-50 ng/mL for sulfonamides, 20-100 ng/mL for tetracyclines, and 30-80 ng/mL for quinolones, and there were no false negative reactions at above these LODs. In addition, all of the LODs of the developed kit were correlated with high-performance liquid chromatography (HPLC) data. Our lateral flow immunoassay kit can simultaneously detect antibiotic residues from a large number of fish samples rapidly, strengthening the safety of domestic farmed and imported fish.
연육 (surimi) 소재로서 어체중량이 다른 넙치의 품질 특성 비교
허민수 ( Min Soo Heu ),신준호 ( Jun Ho Shin ),박권현 ( Kwon Hyun Park ),이지선 ( Ji Sun Lee ),노윤이 ( Yu Ni Noe ),전유진 ( You Jin Jeon ),김진수 ( Jin Soo Kim ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.1
This study investigated the physicochemical and enzymatic properties of unmarketable cultured bastard halibut (Paralichthys olivaceus) of different weights as a potential source of surimi and surimi gel. The proximate composition of cultured bastard halibut of different weights did not differ significantly at P<0.05 (light weight (LBH) 400-500 g, medium weight (MBH) 600-800 g, and heavy weight (HBH) > 1,000 g). Compared to Alaska pollock muscle, the bastard halibut muscle had a 4% higher crude protein content and 6% lower moisture content. The collagen content of LBH bastard halibut muscle was 1.58 g/100 g, which was lower than or no different from bastard halibut weighing different amounts. Regardless of fish weight or pH, the enzymatic activities of crude fish extracts ranged from 0.34-0.48 U/mg for casein and hemoglobin, 11.0-12.7 U/mg for LeuPNA, 5.4-6.1 U/mg for ArgPNA, 2.3-2.9 U/mg for SAAPFNA, and 0.1-0.2 U/mg for BAPNA. The yield of surimi gel from LBH was 24.4%, which was similar to that from MBH and lower than that from HBH. The surimi gel from LBH was similar to that from HBH, while weaker than that from MBH. The surimi gel from LBH gel was stronger than grade SA gel from commercial Alaska pollock.