여드름을 유발하는 Propionibacterium acnes에 대한 분리 세균들의 항균활성 = Antibacterial activity of isolated bacteria against Propionibacterium acnes causing acne vulgaris

국문 초록 (Abstract)

본 연구는 여드름을 유발하는 세균인 Propionibacterium acnes에 대해 다양한 토양에서 분리된 세균 균주들의 항균효과를 조사하기 위해 수행되었다. 수백 개의 분리 세균균주 중 Paenibacillus elgii DS381과 Paenibacillus elgii DS1515, Burkhoderia gladioli DS518, Streptomyces lienomycini DS620는 2가지 균주의 P. acnes에 대해 높은 항균활성을 나타내었다. 이 분리균주들은 agar well diffusion test에서 15.5~34.3 mm 직경의 저해대를 형성하였으며, 특히 DS620는 가장 큰 저해대 직경(28.3~34.3 mm)을 나타내었다. 분리 균주가 생성하는 항균 물질은 DS381과 DS1515 균주의 경우lipopeptide (pelgipeptin, paenipeptin), DS518은 protease, 그리고 DS620은 anthracycline 인 것으로 추정되며, 이들 모두 P. acnes에 대해매우낮은 최소저해농도를 나타내었다[DS381와 DS1515 (0.078 mg/ml), DS518 (0.312 mg/ml), DS620 (0.000078 mg/ml)]. P. acnes를 대상으로 한 time-kill assay에서는 네 균주의 항균물질이 모두 24시간 이내에 P. acnes를 완전히 사멸시켰다. 이 결과는 네 가지 항균활성 균주들이 분비하는 항균물질들이 여드름을 유발하는 P. acnes에 대하여 효율적인 치료 소재로 사용될 수 있는 가능성을 보여준다.

다국어 초록 (Multilingual Abstract)

This study was carried out to evaluate antimicrobial activity of isolated bacteria from various soils against two strains of Propionibacterium acnes causing acne vulgaris. Among several hundreds of bacterial strains, Paenibacillus elgii DS381, Paenibacillus elgii DS1515, Burkholderia gladioli DS518, and Streptomyces lienomycini DS620 showed high antimicrobial activities against the strains of P. acnes. All isolated bacteria showed 15.5 to 34.3 mm inhibition zone diameter in an agar well diffusion test, and especially DS620 showed the highest inhibition zone diameters (28.3~34.3 mm). Antibacterial substances were expected as lipopeptide (pelgipeptin and paenipeptin) from strains DS381 and DS1515, protease from DS518, and anthracycline antibiotic (daunomycinone) from DS620, and all these showed very low minimum inhibitory concentration [DS381 and DS1515 (0.078 mg/ml), DS518 (0.312 mg/ml), DS620 (0.000078 mg/ml)] against P. acnes. These antibacterial substances could completely kill P. acnes within 24 h in a time-kill assay. These results suggest that antibacterial substances produced by these bacteria may be utilized as useful antimicrobial agent against P. acnes and treatment medicine for acne vulgaris.

참고문헌 (Reference)

1 김태완, "해양유래미생물 항균 활성 Bacillus sp. 2-4 균주의 대량 배양방법" 한국수산해양교육학회 30 (30): 123-131, 2018

2 Luan Luong Chu, "Synthetic analog of anticancer drug daunorubicin from daunorubicinone using one-pot enzymatic UDP-recycling glycosylation" Elsevier BV 124 : 1-10, 2016

3 M. M. Sopirala, "Synergy Testing by Etest, Microdilution Checkerboard, and Time-Kill Methods for Pan-Drug-Resistant Acinetobacter baumannii" American Society for Microbiology 54 (54): 4678-4683, 2010

4 Basma Ghorbel, "Stability studies of protease from Bacillus cereus BG1" Elsevier BV 32 (32): 513-518, 2003

5 Sharma D., "Simultaneous production of biosurfactants and bacteriocins by probiotic Lactobacillus casei MRTL3" 2014 : 1-7, 2014

6 K. Arihara, "Salivacin 140, a novel bacteriocin from Lactobacillus salivarius subsp. salicinius T140 active against pathogenic bacteria" Wiley 22 (22): 420-424, 1996

7 Laishram Shantikumar Singh, "Production of potent antimicrobial agent by actinomycete, Streptomyces sannanensis strain SU118 isolated from phoomdi in Loktak Lake of Manipur, India" Springer Nature 14 (14): 2014

8 Issam AL-Ani, "Pharmacological synergism of bee venom and melittin with antibiotics and plant secondary metabolites against multi-drug resistant microbial pathogens" Elsevier BV 22 (22): 245-255, 2015

9 Huang E, "New Paenibacillus strain produces a family of linear and cyclic antimicrobial lipopeptides: cyclization is not essential for their antimicrobial activity" 364 : 1-6, 2017

10 Murugan G., "Isolation, screening and production of biosurfactant by Pseudomonas sp. isolated from mangrove forest soil using coconut oil cake as substrate" 5 : 251-257, 2011

11 E. V. FOMICHOVA, "Isolation and identification of new anthracycline antibiotics, rubomycins F and H." Japan Antibiotics Research Association 45 (45): 1185-1186, 1992

12 M. Nagarajkumar, "Involvement of secondary metabolites and extracellular lytic enzymes produced by Pseudomonas fluorescens in inhibition of Rhizoctonia solani, the rice sheath blight pathogen" Elsevier BV 159 (159): 73-81, 2004

13 Vowels BR, "Induction of proinflammatory cytokines by a soluble factor of Propionibacterium acnes: Implications for chronic inflammatory acne" 63 : 3158-3165, 1995

14 Choi JS, "In vitro antibacterial and anti-inflammatory properties of seaweed extracts against acne inducing bacteria, Propionibacterium acnes" 32 : 313-318, 2013

15 임영희, "In vitro Activity of Kaempferol Isolated from the Impatiens balsamina alone and in Combination with Erythromycin or Clindamycin against Propionibacterium acnes" 한국미생물학회 45 (45): 473-477, 2007

16 A. Nostro, "Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity" Wiley 30 (30): 379-384, 2000

17 Sejong Oh, "Effect of bacteriocin produced by Lactococcus sp. HY 449 on skin-inflammatory bacteria" Elsevier BV 44 (44): 1184-1190, 2006

18 Steenbergen JN, "Daptomycin: a lipopeptide antibiotic for the treatment of serious Gram-positive infections" 55 : 283-288, 2005

19 J I Ross, "Clinical resistance to erythromycin and clindamycin in cutaneous propionibacteria isolated from acne patients is associated with mutations in 23S rRNA." American Society for Microbiology 41 (41): 1162-1165, 1997

20 Jie Jiang, "Characterization of a novel bacteriocin produced by Lactobacillus sakei LSJ618 isolated from traditional Chinese fermented radish" Elsevier BV 23 (23): 338-344, 2012

21 Ansari A, "Bacteriocin (BAC-IB17): Screening, isolation and production from Bacillus subtilis KIBGE IB-17" 25 : 195-201, 2012

22 Mullika Traidej Chomnawang, "Antimicrobial effects of Thai medicinal plants against acne-inducing bacteria" Elsevier BV 101 (101): 330-333, 2005

23 Luangnarumitchai S, "Antimicrobial activity of essential oils against five strains of Propionibacterium acnes" 34 : 60-64, 2007

24 Bong Seon Kang, "Antimicrobial Activity of Enterocins from Enterococcus faecalis SL-5 against Propionibacterium acnes, the Causative Agent in Acne Vulgaris, and Its Therapeutic Effect" 한국미생물학회 47 (47): 101-109, 2009

25 Carl Erik Nord, "Antibiotic resistance in Propionibacterium acnes. Microbiological and clinical aspects" Elsevier BV 12 (12): 207-210, 2006

26 P. Niyomkam, "Antibacterial activity of Thai herbal extracts on acne involved microorganism" Informa UK Limited 48 (48): 375-380, 2010

27 Andrew Desbois, "Antibacterial Activity of Long-Chain Polyunsaturated Fatty Acids against Propionibacterium acnes and Staphylococcus aureus" MDPI AG 11 (11): 4544-4557, 2013

28 Gal SW, "Anti-pimple cosmetic resource of culture broth of Paecilomyces japonica mycelium which was incubated with mulberry leaf powder and inhibited the growth of Propionibacterium acnes. Korea Patent KR 1009555630000"

29 Jayaraman P, "Activity and interactions of antibiotic and phytochemical combinations against Pseudomonas aeruginosa in vitro" 6 : 556-568, 2010

30 Yuangang Zu, "Activities of Ten Essential Oils towards Propionibacterium acnes and PC-3, A-549 and MCF-7 Cancer Cells" MDPI AG 15 (15): 3200-3210, 2010

31 G. F Webster, "Acne vulgaris * Commentary: A UK primary care perspective on treating acne" BMJ 325 (325): 475-479, 2002