[SESSION PE 포스터-방지기술]PE16) 질소 도핑을 통한 이산화티타늄 제조 및 수은의 제어

현정은,이태규,배귀남,정종수 한국대기환경학회 2004 한국대기환경학회 학술대회논문집 Vol.- No.-

천연 항균물질이 첨가된 소금의 식중독 세균에 대한 항균효과

현정은,박세은,이서현,이연진,장민경,문성권,이선영,Hyun, Jeong-Eun,Park, Se-Eun,Lee, Seo-Hyeon,Lee, Yeon-Jin,Jang, Min-Kyung,Moon, Sung-Kwon,Lee, Sun-Young 한국급식외식위생학회 2020 급식외식위생학회지 Vol.1 No.1

Salt is a common seasoning agent used in various processed foods, especially in kimchi and salted seafood (jeotgal). This study was conducted to investigate the efficacy of salt with antimicrobial substances (acetic acid, garlic extract, carvacrol, nisin, thymol, and their combination (acetic acid+nisin+thymol)) on improvement of antibacterial effects of salt against foodborne pathogens. Salt (10%) was prepared using six different types of 0.2% natural antimicrobial substances. The antibacterial effect of salt combined with natural antimicrobial substances was evaluated against foodborne pathogens using the broth micro-dilution method and growth curve plotted using absorbance measurements. For the five foodborne pathogens, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of salt without antimicrobial substances as control were in the range of 24~>50,000 ㎍/mL and >50,000 ㎍/mL, respectively. Salt with nisin, thymol, or garlic extract showed strong inhibitory effects and their MIC against L. monocytogenes were 49, 12,500, and 24 ㎍/mL, respectively. In particular, salt with nisin showed inhibitory activities against Gram-positive bacteria. However, all the antimicrobial substances were less effective against Gram-negative bacteria such as E. coli O157:H7 and S. Typhimurium than Gram-positive bacteria. These results could be used for the development of salt with natural antimicrobial substances especially targeted against L. monocytogenes. This would enable the lowering of saline concentration while improving the storability of food.

토론 및 결론

현정은,김현숙 팬코리아영어교육학회(구 영남영어교육학회) 2002 영어교육연구 Vol.14 No.2

전처리 농산물의 유통 경로에 따른 미생물학적 오염도 조사

현정은,이수빈,정도영,채효빈,최송이,황인준,김세리 한국식품영양과학회 2023 한국식품영양과학회지 Vol.52 No.3

This study investigates microbial contamination in pre-treated agricultural products. Eleven types of pre-treated agricultural product were evaluated for microbial contamination arising from four distribution channels: supermarket, local market, online, and traditional market. Of the 38 samples examined, the average populations of total aerobic bacteria and coliform in sliced garlic were higher by 6.85 and 5.94 log CFU/g, respectively. Similarly, the average populations of total aerobic bacteria and coliform determined in shredded green onion were 6.39 and 5.46 log CFU/g, respectively. In particular, the average populations of total aerobic bacteria were higher in sliced garlic and shredded green onion than in peeled garlic and peeled green onion. Furthermore, Bacillus cereus (13.16%) was detected in peeled chestnut, peeled potato, cut Chinese yam, and cut green onion. In addition, Staphylococcus aureus was isolated from cut radish in 1 out of 38 samples (2.63%). However, no correlation was obtained between microbial population and distribution channel in all samples. These results could provide the basic information to control the microbial safety of pre-treated agricultural products.

미생물 생체모방 기반의 단백질 상호작용을 응용한 고효율 효소 복합체 설계

현정은,선하빈 한국생물공학회 2021 BT NEWS Vol.28 No.1

중학교 영어교과서에 반영된 문화 분석

현정은,김현숙 팬코리아영어교육학회(구 영남영어교육학회) 2002 영어교육연구 Vol.14 No.2

Comparison of measurement methods at determining the target sites injured by antimicrobials in Escherichia coli O157:H7 using metabolic inhibitors

현정은,이선영 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.8

Acquiring an understanding of the mechanisms underlying antimicrobial action is important for overcom- ing bacterial resistance to antimicrobials. This study eval- uated three different methods (antimicrobial fixed broth dilution method, metabolic inhibitors fixed broth dilution method, and metabolic inhibitor fixed agar recovery method) for determining the target site of Escherichia coli O157:H7 by treatments with various antimicrobials (etha- nol, ethylenediaminetetraacetic acid, polymyxin B, thymol, acetic acid, and citrus fruit extract). However, the results indicated only weak relationships between MIC values and mechanisms of antimicrobials known to cause damage or injury. In addition, the results of three measurement methods using metabolic inhibitors were not correlated. These results suggest that measurement methods using metabolic inhibitors alone may not be suitable for deter- mining the target site injured by antimicrobials. Therefore, various measurement methods should be compared and analyzed to determine the damage or injury sites targeted by antimicrobials in pathogenic bacteria. Further studies are needed to compare and analyze the various measure- ment methods for determining the target site injured by antimicrobials in pathogenic bacteria

국외 식중독으로 인한 손실비용 추정을 위한 항목 비교 연구

현정은,진현정,김예솔,주효정,강우인,이선영 한국식품위생안전성학회 2021 한국식품위생안전성학회지 Vol.36 No.1

식중독에 의한 사회경제적 손실을 최소화하기 위해서는 식중독과 관련된 경제적 비용 손실을 추정하는 것이 중요하다. 하지만 자료의 부족과 다양한 비용 항목의 존재로 식중독과 관련된 사회경제적 손실비용을 정확하게 추정하기에 어려운 점 있다. 본 연구에서는 국내 식중독 발생에 의한 손실비용을 추정하기에 앞서 국외에서 사용되는 사회경제적 비용항목의 기반 자료 확보를 목적으로 수행되었다. 2009-2019년 국외 식중독과 관련된 사회경제적 손실비용 측정 연구의 사례 조사를 위해 문헌 조사를 실시하였다. 이 연구에서는 사회경제적 손실비용을 크게 의료 기간에 방문한 외래/입원환자 및 의료기관에 미방문한 경험환자로 구분하였다. 또한 이들의 직접비용 및 간접비용을 고려하였고, 더 나아가 기업비용 및 행정비용 항목으로 구분하여 조사하였다. 조사 결과 문헌별, 나라별 상이한 비용항목을 사용하여 손실비용을 추정하는 것으로 관찰되었다. 직접의료비의 경우 모든 연구에서 외래 또는 입원진료비를 선정하여 비용을 산출한 것으로 관찰되었기 때문에 나라별 의료서비스의 체제 및 비용에 따라 항목을 선정해야 한다. 직접비의료비의 경우 몇 몇의 연구에서 외래 방문에 소요된 교통비만을 고려하였기 때문에 간병비 고려 여부에 대해서는 더 논의가 필요할 것으로 사료된다. 간접비용 중 조기사망비용, 작업손실비용, 여가손실비용 및 삶의 질 저하/고통비용은 고려하였으나, 병문안 기회비용은 모든 연구에서 고려하지 않았다. 직접의료비와 마찬가지로 행정비용의 경우에도 국가별 정부 예산이 상이하기 때문에 각 나라에 맞는 항목을 고려해야할 것이다. 따라서 이러한 조사 결과를 바탕으로 국내 식중독 발생에 의한 사회경제적 손실비용 분석을 위해 어떠한 비용 항목을 고려해야 할 것인지에 대한 검증절차가 필요할 것이다. 본 연구는 국내 식중독 발생에 의한 사회경제적 손실비용 분석을 위한 기초적인 정보를 제공할 것이다. Foodborne outbreaks frequently occur worldwide and result in huge economic losses. It is the therefore important to estimate the costs associated with foodborne diseases to minimize the economic damage. At the same time, it is difficult to accurately estimate the economic loss from foodborne disease due to a wide variety of cost components. In Korea, there are a limited number of analytical studies attempting to estimate such costs. In this study we investigated the components of economic cost used in foreign countries to better estimate the cost of foodborne disease in Korea. Seven recent studies investigated the cost components used to estimate the cost of foodborne disease in humans. This study categorized the economic loss into four types of cost: direct costs, indirect costs, food business costs, and government administration costs. The healthcare costs most often included were medical (outpatient) and hospital costs (inpatient). However, these cost components should be selected according to the systems and budgets of medical services by country. For non-healthcare costs, several other studies considered transportation costs to the hospital as an exception to the cost of inpatient care. So, further discussion is needed on whether to consider inpatient care costs. Among the indirect costs, premature mortality, lost productivity, lost leisure time, and lost quality of life/ pain, grief and suffering costs were considered, but the opportunity costs for hospital visits were not considered in any of the above studies. As with healthcare costs, government administration costs should also be considered appropriate cost components due to the difference in government budget systems, for example. Our findings will provide fundamental information for economic analysis associated with foodborne diseases to improve food safety policy in Korea.

Kombucha Tea Prevents Obese Mice from Developing Hepatic Steatosis and Liver Damage

현정은,이영재,왕시형,김진년,김지은,차재호,서영수,정영미 한국식품과학회 2016 Food Science and Biotechnology Vol.25 No.3

Nonalcoholic fatty liver disease (NAFLD) is associated with the increased accumulation of hepatocellular lipids. Although Kombucha tea (KT) has emerged as a substance protecting the liver from damage, the effects of KT in NAFLD remain unclear. Hence, we investigated whether KT influenced hepatic steatosis. Db/db mice were fed either control or methionine/choline-deficient (MCD) diets for 4 weeks. The MCD diet group was treated with KT or water for 3 weeks. KT treatment alleviated macrovesicular steatosis compared to the MCD-fed group. The levels of triglyceride, ALT, and AST also decreased in the KT+MCD-treated db/db mice. RNA expression in the MCD+KT group showed reduced triglyceride synthesis and uptake of fatty acids. Immunostaining and western blot assays for active caspase-3 demonstrated a lower level of apoptosis in the MCD+KT than in the MCD group. These results demonstrate that KT attenuated lipid accumulation and protected the liver from damage, promoting liver restoration in mice.

PE14)전기가열 튜브로를 이용한 광촉매 TiO₂ 입자의 제조 및 촉매 특성 분석

현정은,배귀남,이태규 한국대기환경학회 2003 한국대기환경학회 학술대회논문집 Vol.- No.-