Enhancement of Parathyroid Hormone in Postmenopausal Women by Chlorella Dietary Supplementation

Kim, Dong-Uk,Seong, Hee-Kyung,Hwang, Jung-Min,Jeon, Ae-Ran,Yun, Ji-Young,Kim, Yong-Ho 대한의생명과학회 2003 Journal of biomedical laboratory sciences Vol.9 No.1

Parathyroid hormone has clearly emerged as the most promising new anabolic treatment for osteoporosis by increasing the activation of osteoblast. It is known that chlorella increases both bone mineral density (BMD) and the rate of bone formation. The purpose of the present study was to determine whether the chlorella dietary supplementation could effect the thyroid or parathyroid hormones associated with increased BMD and bone formation. Twenty-two postmenopausal woman were treated for four month with 4 gm of chlorella dietary supplementation per day, then assessed serum calcium,25 OH vitamin D$_3$, thyroid hormone and parathyroid hormone before and after treatment. The mean 25 OH vitamin D$_3$ and parathyroid hormone were shown to marked increases by 193% and 265% respectively, in contrast to decreases by 9.4%, 37%, 33% and 14% in serum calcium, triiodo-thyroxine, free thyroxine and thyroxine stimulation hormone. In conclusion, treatment of postmenopausal women with chlorella dietary supplementation resulted in an increase in BMD and bone formation through enhancement of parathyroid hormone and 25 OH vitamin D$_3$, and a decrease in thyroid hormones.

Enhancement of Parathyroid Hormone in Postmenopausal Women by Chlorella Dietary Supplementation

Kim, Dong-Uk,Seong, Hee-Kyung,Hwang, Jung-Min,Jeon, Ae-Ran,Yun, Ji-Young,Kim, Yong-Ho 대한의생명과학회 2003 Biomedical Science Letters Vol.9 No.1

Parathyroid hormone has clearly emerged as the most promising new anabolic treatment for osteoporosis by increasing the activation of osteoblast. It is known that chlorella increases both bone mineral density (BMD) and the rate of bone formation. The purpose of the present study was to detemine whether the chlorella dietary supplementation could effect the thyroid or parathyroid hormones associated with increased BMD and bone formation. Twenty-two postmenopausal woman were treated for four month with 4 gm of chlorella dietary supplementation per day, then assessed serum calcium, 25 OH vitamin D_3, thyroid hormone and parathyroid hormone before and after treatment. The mean 25 OH vitamin D_3 and parathyroid hormone were shown to marked increases by 193% and 265% repectively, in contrast to decreases by 9.4%, 37%, 33% and 14% in serum calcium, triiodo-thyroxine, free thyroxine and thyroxine stimulation hormone, In conclusion, treatment of postmenopausal women with chlorella dietary supplementation resulted in an increase in BMD and bone formation through enhancement of parathyroid hormone and 25 OH vitamin D_3, and a decrease in thyroid hormones.

고칼슘혈증을 보인 폐암환자에서 부갑상선호르몬(PTH)과 부갑상선호르몬관련단백질(PTHrP)이 동시에 증가된

김유일 ( Yu Il Kim ),김규식 ( Kyu Sik Kim ),유영권 ( Young Kwon Yu ),박창민 ( Chang Min Park ),임명수 ( Myung Soo Rim ),고경행 ( Kyung Haeng Ko ),황준화 ( Jun Hwa Hwang ),박형관 ( Hyeong Kwan Park ),임성철 ( Sung Chul Lim ),김영철 대한결핵 및 호흡기학회 1999 Tuberculosis and Respiratory Diseases Vol.47 No.4

부갑상선 호르몬 연관 단백 (Parathyroid Hormone-related Protein) 1-34가 토끼 방광근 수축에 미치는 영향

손환철,남현우,오승준,이안기,김광명,최황 大韓泌尿器科學會 2002 Korean Journal of Urology Vol.43 No.3

부갑상선 호르몬 관련 펩티드를 분비하는 간세포암종의 완전 절제

김은경 ( Eun Kyoung Kim ),김진수 ( Jin Su Kim ),신기철 ( Ki Chul Shin ),이길태 ( Gil Tae Lee ),한철주 ( Chul Ju Han ),김상범 ( Sang Beom Kim ),구윤희(교신저자) ( Yun Hyi Ku ) 대한소화기학회 2015 대한소화기학회지 Vol.66 No.2

Hepatocellular carcinoma (HCC) is the fifth most common cancer in Korea. Diverse paraneoplastic syndromes can occur in patients with HCC, but parathyroid hormone-related peptide (PTH-rP)-induced hypercalcemia is uncommon. Hypercalcemia due to PTH or particularly PTH-rP-secreting HCC is associated with poor outcomes. We report a 71-year-old man who presented with symptoms of vague abdominal discomfort, somnolence, lethargy, nausea, vomiting, and weight loss. Imaging studies revealed a large HCC without metastasis. The laboratory findings showed elevated serum calcium level, low intact parathyroid hormone (iPTH) level and elevated PTH-rP level. These results led to a diagnosis of a PTH-rP-secreting HCC and paraneoplastic hypercalcemia. After emergency management of the hypercalcemia, the patient underwent an extended right hemihepatectomy with cholecystectomy. One year after the surgery, he is alive with normal calcium, PTH-rP, and iPTH levels. This case demonstrates that the rare phenomenon of life-threatening hypercalcemia caused by HCC should not be overlooked. These symptoms offer a good opportunity to diagnose HCC early. Radical tumor resection makes it possible to cure patients with PTH-rP-secreting HCC. (Korean J Gastroenterol 2015;66:122-126)

Odontogenic Potential of Parathyroid Hormone–related Protein (107-111) Alone or in Combination with Mineral Trioxide Aggregate in Human Dental Pulp Cells

Han, Jeong-Won,Lee, Bin-Na,Kim, Se-Min,Koh, Jeong-Tae,Min, Kyung-San,Hwang, Yun-Chan Elsevier 2017 JOURNAL OF ENDODONTICS - Vol.43 No.12

<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Parathyroid hormone–related protein plays an important role in bone remodeling. Its N-terminal domain parathyroid hormone–related protein (107-111) is called osteostatin (OST). OST has demonstrated osteogenic potential when combined with biomaterials such as hydroxyapatite or bioceramics. However, the odontogenic potential of OST has not yet been reported. Therefore, the aim of this study was to determine whether OST has an odontogenic effect or a synergistic effect with mineral trioxide aggregate (MTA) in human dental pulp cells (hDPCs) and to examine the underlying signaling mechanisms involved in OST-mediated odontogenic differentiation.</P> <P><B>Methods</B></P> <P>Viability of hDPCs on stimulation with OST or MTA was measured. Real-time polymerase chain reaction and Western blot analyses were performed to evaluate the expression levels of odontogenic markers and the activation of extracellular signal-regulated kinase (ERK). To evaluate mineralized nodule formation, alkaline phosphatase (ALP) staining and alizarin red S staining were performed. Combined effects of OST and MTA were evaluated.</P> <P><B>Results</B></P> <P>OST promoted odontogenic differentiation, as evidenced by the formation of mineralized nodules, induction of ALP activity, and upregulation of odontogenic markers (dentin sialophosphoprotein, dentin matrix protein-1, and ALP). Phosphorylation of ERK was increased by OST. However, ERK inhibitor (U0126) inhibited the increase in dentin sialophosphoprotein and dentin matrix protein-1 expression and mineralization induced by OST. A combination of MTA and OST upregulated odontogenic differentiation-associated gene expression and calcium nodule mineralization in hDPCs compared with MTA alone.</P> <P><B>Conclusions</B></P> <P>The present study revealed that OST can promote odontogenic differentiation and mineralization through activating the ERK signaling pathway. A combination of MTA and OST showed a synergistic effect compared with MTA alone in hDPCs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> OST promotes odontogenic differentiation through ERK signaling pathway in hDPCs. </LI> <LI> Combination of MTA and OST has synergistic effect compared with MTA alone in hDPCs. </LI> <LI> OST can be used as an therapeutic agent for pulp capping by itself or in combination with MTA. </LI> </UL> </P>

혈액투석 환자에서 혈청 마그네슘 농도와 임상적 의의

조종태 ( Jo Jong Tae ) 대한신장학회 2003 Kidney Research and Clinical Practice Vol.22 No.5

목 적 : 혈액투석 환자에서 고마그네슘혈증은 신성골이양증에 관여할 수도 있다는 부정적 측면과 부갑상선호르몬 상승을 억제할 수도 있다는 긍정적 측면이 보고되고 있지만 분명한 임상적 의의는 잘 알려져 있지 않다. 따라서 본 연구의 목적은 혈액투석 환자에서 혈청 마그네슘 농도와 부갑상선호르몬과의 관계를 각종 임상적 지표들과 함께 분석하여 혈액투석 환자에서 혈청 마그네슘 농도와 임상적 의의를 규명하는 것이다. 방 법 : 현재 단국대학교병원에서 말기신부전으로 혈액투석 (투석액의 마그네슘 농도는 1.5 mEq/L) 치료 중인 환자 81명 (연령은 53±12세, 남녀비는 38:43, 투석기간은 36±35개월, 원인신질환은 당뇨병성신증이 30%)을 대상으로 혈청 마그네슘과 부갑상선호르몬 농도 및 각종 임상적 지표들을 연속해서 측정하여 분석하였다. 결 과 : 혈청 마그네슘 농도는 2.78±0.45 mEq/L (범위 1.50-3.95 mEq/L)이었고 고마그네슘혈증 (serum Mg >2.1 mEq/L)은 72명 (89%)이었으며 부갑상선호르몬 농도 (intact PTH)는 128±224 pg/mL (범위 3-1,344 pg/mL)이었다. 중등도 이상의 고마그네슘혈증 (serum Mg ≥3.0 mEq/L)이 있는 군 (n=28)에서 혈청 부갑상선호르몬 농도가 의미있게 낮았고 알루미늄 농도는 의미있게 높았다. 상대적 부갑상선기능저하증 (iPTH <120 pg/mL)이 있는 군 (n=55)의 혈청 마그네슘, 알루미늄 및 칼슘 농도가 의미있게 높았고 alkaline phosphatase는 의미있게 낮았으며 혈청 마그네슘, 알루미늄 및 칼슘 농도는 부갑상선호르몬 농도와 각각 음의 상관관계 (r=-0.27, -0.31, -0.28, p<0.05)를 보였고 다중회귀분석결과 혈청 부갑상선호르몬 농도를 예견할 수 있었다 (multiple r=0.39, p<0.05). 결 론 : 혈액투석 환자에서 고마그네슘혈증은 흔한 현상이고 중등도 이상의 고마그네슘혈증이 동반됐을 경우에는 고알루미늄혈증이 동반됐을 가능성을 생각해볼 필요가 있다. 또한 상대적 부갑상선기능저하증이 있는 환자들에게는 높은 농도의 혈청 마그네슘이 부갑상선호르몬의 생성 혹은 분비를 억제하는 효과가 있는 것으로 사료된다. 이러한 결과는 혈액투석 환자에서 고마그네슘혈증이 무력성 골질환과 같은 신성골이양증의 발병에 관여할 가능성을 시사한다. Background : Hypermagnesemia may be implicated to have both harmful and beneficial effects in dialysis patients. It may contribute to osteomalacic renal osteodystrophy and suppression of parathyroid hormone. The purposes of this study were to analyze the serum magnesium concentration in hemodialysis patients, and to clarify the relationship between serum magnesium and intact parathyroid hormone concentration (iPTH) independently of other clinical parameters. Methods : Eighty-one patients (38 males and 43 females) with end-stage renal failure undergoing maintenance hemodialysis in Dankook University Hospital were included in this study. The mean age was 53+12 years and the duration of dialysis was 36k35 months. The underlying kidney disease was diabetic nephropathy in 24 patients (30%). The patients used a dialysate magnesium concentration of 1.5 mEq/L. The serum magnesium, iPTH and other clinical parameters were evaluated and analyzed. Results : The mean serum magnesium concentration was 2.78±0.45 mEq/L (range 1.50-3.95 mEq/L). Hypermagnesemia (defined as serum Mg >2.1 mEq/L) was found in 72 patients (89%). The mean iPTH was 128±224 pg/mL (range 3-1,344 pg/mL). The iPTH was significantly low, and the serum aluminum concentration was significantly high in patients (n=28) with more severe hypermagnesemia (defined as serum Mg >3.0 mEq/L). The serum magnesium, aluminum and calcium concentration have significant negative correlations with iPTH respectively (r=-0.27, -0.31, -0.28, p<0.05) in patients (n=55) with relative hypoparathyroidism (defined as iPTH <120 pg/mL). Conclusion : Hypermagnesemia was common in hemodialysis patients. High serum aluminum concentration should be considered in patients with moderate to severe hypermagnesemia. Furthermore, hypermagnesemia as well as high serum aluminum and calcium concentration may have a suppressive effect on FTH in patients with relative hypoparathyroidism or adynamic bone disease.

12주간 복합운동이 여성 노인의 신체수행능력, 골밀도 및 부갑상선호르몬에 미치는 영향

김정숙,김지현,하수민,현수진,윤병곤,김도연 한국응용과학기술학회 2018 한국응용과학기술학회지 Vol.35 No.2

The purpose of this study was to investigate the effects of combined exercise program on short physical performance battery, bone mineral density and parathyroid hormone levels in elderly women. This study subjects were 22 elderly female volunteers, aged 65–75 years and they divided into the combined exercise group(n=11) and the "no exercise" control group(n=11). The combined exercise included the line dance program performed twice per week for 60 minutes and the resistance exercise program performed once per week during 12 weeks. Exercise intensity was progressively increased from RPE “fairly light” to “slightly hard” (from 11 to 14). The findings of this study were as follows; Short physical performance battery(Balance test, gait speed, and rising from a chair five times) and bone mineral density (lumbar spine L2~L4 bone densities, and the T-score) were significantly increased after 12 weeks in the exercise group compared to the baseline. The PTH level was significantly decreased in the exercise group compared to the baseline. In conclusion, regular and continuous physical activity was effective for improving short physical performance and had a positive effect on bone mineral density and parathyroid hormone. With aging, women have decreased muscle strength and bone density and therefore, it is strongly recommended that women need to carry out continuous physical activity to prevent disease and ensure a healthy retirement. 본 연구는 여성노인을 대상으로 라인댄스와 저항성운동을 적용한 복합운동프로그램이 신체수행 능력, 골밀도 및 부갑상선호르몬에 미치는 영향을 구명하기 위하여, 만65세~75세 여성노인을 대상으로 운동군 11명, 대조군 11명으로 분류하여 회당 60분씩 주 2회 라인댄스, 1회 저항성운동을 12주간 실시하였 다. 운동 수행강도는 운동자각도(RPE)를 이용하여 ‘가볍다’에서 ‘약간 힘들다’ 정도인 11~14 사이를 유지 하였다. 라인댄스와 저항성운동 전·후에 측정한 자료의 그룹 내 차이 비교를 위해 대응표본 T검정, 그룹 간차이는 운동 전·후의 변화량을 산출하여 독립표본 T검정을 실시하였고, 측정변인들에 대한 그룹 및 시기간 상호작용을 검증하기 위해 이원배치 반복측정 분산분석으로 하였으며, 각 항목별 통계적 유의수준은 .05로 설정하였다. 그 결과, 운동군이 신체수행능력 중 균형(p<.05), 보행속도(p<.01), 의자에서 일어서기 (p<.001), SPPB 종합점수(p<.001)가 증가하였으며, 골밀도는 요추(L2~L4)골밀도에서 운동군이 유의하게 증가하였고(p<.05), T-score점수는 운동군이 유의한 증가를 보였으나(p<.01), 대조군은 유의한 차이가 나타나지 않았다. PTH수준은 운동군이 유의하게 감소하였고(p<.05), 대조군은 유의한 차이가 나타나지 않았 다. 이상의 결과 규칙적이고 지속적인 신체활동은 여성노인의 신체수행능력, 골밀도 및 부갑상선 호르몬의 변화에 긍정적인 영향을 미친 것으로 사료된다.

만성 콩팥병 환자에서 추정 사구체 여과율에 따른 25-Hydroxyvitamin D의 특성

김태균 ( Tae Kyun Kim ),이수봉 ( Soo Bong Lee ),박창준 ( Chang Jun Park ),성은영 ( Eun Young Seong ),곽임수 ( Ihm Soo Kwak ),송상헌 ( Sang Heon Song ),고정희 ( Jung Hee Koh ),이하린 ( Harin Rhee ),오상보 ( Sang Bo Oh ) 대한내과학회 2012 대한내과학회지 Vol.83 No.6

Background/Aims: Accumulating data suggest that vitamin D deficiency is prevalent in patients with chronic kidney disease (CKD). However, comprehensive data are lacking for Koreans. The aim of this study was to survey vitamin D deficiency among patients with CKD in Korea and to identify the relationships among various factors. Methods: We conducted a retrospective cohort study of 444 patients who were divided into four subgroups by estimated glomerular filtration rate (eGFR) for comparisons of mean 25-hydroxyvitamin D [25(OH)D] level and other parameters. In addition, non-dialyzed patients were categorized into four groups based on 25(OH)D levels (<10, 10-19, 20-29, and ≥ 30 ng/mL), and risk factors for severe vitamin D deficiency (<10 ng/mL) were investigated. Results: Of patients with an eGFR ≥ 60 mL/min/1.73 m2, 43% (34/79) showed severe 25(OH)D deficiency, and the mean 25(OH)D level was 11.7±5.3 ng/mL. In CKD3 group, 53.2% (41/77) showed severe 25(OH)D deficiency, with a mean level of 11.3±7.2 ng/mL. In CKD4 group, 53.3% (49/92) had severe 25(OH)D deficiency, with a mean level of 11.0±6.2 ng/mL. Approximately 71% (139/196) of patients in CKD5 group showed severe deficiency, and the mean level was 9.2±5.9 ng/mL. Severe 25(OH)D deficiency was affected by winter season, renal function, diabetes, and low-density lipoprotein cholesterol. The serum parathyroid hormone level was inversely correlated with the 25(OH)D level, such that 25(OH)D < 20 ng/mL were associated with a steep increase in parathyroid hormone. Conclusions: Vitamin D deficiency is highly prevalent in the Korean population. Few patients met a sufficient 25(OH)D concentration, even in the early stages of CKD. Our data suggest that 25(OH)D level of 20 ng/mL is a threshold for a rapid increase in parathyroid hormone levels. (Korean J Med 2012;83:740-751)

The N- and C-terminal domains of parathyroid hormone-related protein affect differently the osteogenic and adipogenic potential of human mesenchymal stem cells

Antonio Casado-Díaz,Raquel Santiago-Mora,José Manuel Quesada 생화학분자생물학회 2010 Experimental and molecular medicine Vol.42 No.2

Parathyroid hormone-related protein (PTHrP) is synthesized by diverse tissues, and its processing produces several fragments, each with apparently distinct autocrine and paracrine bioactivities. In bone, PTHrP appears to modulate bone formation in part through promoting osteoblast differentiation. The putative effect of PTH-like and PTH-unrelated fragments of PTHrP on human mesenchymal stem cell (MSCs) is not well known. Human MSCs were treated with PTHrP (1-36)or PTHrP (107-139) or both (each at 10 nM) in osteogenic or adipogenic medium, from the start or after 6days of exposure to the corresponding medium, and the expression of several osteoblastogenic and adipogenic markers was analyzed. PTHrP (1-36) inhibited adipogenesis in MSCs and favoured the expression of osteogenic early markers. The opposite was observed with treatment of MSCs with PTHrP (107-139). Moreover, inhibition of the adipogenic differentiation by PTHrP (1-36) prevailed in the presence of PTHrP (107-139). The PTH/PTHrP type 1 receptor (PTH1R)gene expression was maximum in the earlier and later stages of osteogenesis and adipogenesis, respectively. While PTHrP (107-139) did not modify the PTH1R overexpression during adipogenesis, PTHrP (1-36) did inhibit it; an effect which was partially affected by PTHrP (7-34), a PTH1R antagonist, at 1 μM. These findings demonstrate that both PTHrP domains can exert varying effects on human MSCs differentiation. PTHrP (107-139) showed a tendency to favor adipogenesis,while PTHrP (1-36) induced a mild osteogenic effect in these cells, and inhibited their adipocytic commitment. This further supports the potential anabolic action of the latter peptide in humans.