In Vitro and In Vivo Evaluation of Ranitidine Hydrochloride Loaded Hollow Microspheres in Rabbits

Wei, Yu-Meng,Zhao, Ling 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.10

The objective of this investigation was to develop the hollow micro spheres as a new dosage form of floating drug delivery systems with prolonged stomach retention time. Hollow microspheres containing ranitidine hydrochloride (RH) were prepared by a novel solvent diffusion-evaporation method using ethyl cellulose (EC) dissolved in a mixture of ethanol and ether (6: 1.0, v/v). The yield and drug loading amount of hollow micro spheres were $83.21{\pm}0.28%$ and $20.7{\pm}0.32%$, respectively. The in vitro release profiles showed that the drug release rate decreased with increasing viscosity of EC and the diameter of hollow microspheres, while increased with the increase of RH/EC weight ratio. Hollow microspheres could prolong drug release time (approximately 24 h) and float over the simulate gastric fluid for more than 24 h. Pharmacokinetic analysis showed that the bioavailability from RH-hollow micro spheres alone was about 3.0-times that of common RH gelatin capsules, and it was about 2.8-times that of the solid microspheres. These results demonstrated that RH hollow micro spheres were capable of sustained delivery of the drug for longer period with increased bioavailability.

In Vitro and In Vivo Evaluation of Ranitidine Hydrochloride Loaded Hollow Microspheres in Rabbits

Yu-meng Wei,Ling Zhao 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.10

The objective of this investigation was to develop the hollow microspheres as a new dosage form of floating drug delivery systems with prolonged stomach retention time. Hollow microspheres containing ranitidine hydrochloride (RH) were prepared by a novel solvent diffusion-evaporation method using ethyl cellulose (EC) dissolved in a mixture of ethanol and ether (6:1.0, v/v). The yield and drug loading amount of hollow microspheres were 83.21±0.28% and 20.71±0.32%, respectively. The in vitro release profiles showed that the drug release rate decreased with increasing viscosity of EC and the diameter of hollow microspheres, while increased with the increase of RH/EC weight ratio. Hollow microspheres could prolong drug release time (approximately 24 h) and float over the simulate gastric fluid for more than 24 h. Pharmacokinetic analysis showed that the bioavailability from RH-hollow microspheres alone was about 3.0-times that of common RH gelatin capsules, and it was about 2.8-times that of the solid microspheres. These results demonstrated that RH hollow microspheres were capable of sustained delivery of the drug for longer period with increased bioavailability.

Development and Characterization of a Specific Anti-Caveolin-1 Antibody for Caveolin-1 Functional Study in Human, Goat and Mouse

Ke, Meng-Wei,Jiang, Yan-Nian,Li, Yi-Hung,Tseng, Ting-Yu,Kung, Ming-Shung,Huang, Chiun-Sheng,Cheng, Winston Teng-Kuei,Hsu, Jih-Tay,Ju, Yu-Ten Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.6

Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will be useful for functional study of Caveolin-1 in different tissues. In this study, we generated a rabbit polyclonal antibody that specifically recognizes the N-terminal amino acids 50-65 of Caveolin-1. This polyclonal antibody specifically reacted with Caveolin-1 extracted from cells of different species, including human epithelial A431 cells, goat primary mammary epithelial cells and mice fibroblast NIH 3T3 cells, by Western blotting. Endogenous Caveolin-1 protein expressing in cells and normal human tissues was detected by this polyclonal antibody using immunocytofluorescent and immunohistochemical staining, respectively. Furthermore, an apparent decrease in Caveolin-1 expression in tumorous breast and colon tissues was detected by this polyclonal antibody. In conclusion, we have identified amino acids 50-65 of Caveolin-1, which contains an epitope that is specific to Caveolin-1 and is conserved in the human, goat and mouse. In future, this anti-Caveolin-1 antibody can be used to examine the progression of breast and colon cancers and to study functions of Caveolin-1 in human, goat and mouse cells.

Enhanced Performances of Vertical-Structured Green-Band InGaN/GaN Multiple-Quantum-Well Solar Cells with Aluminum Reflectors

Meng-Hua Lai,Zhi-Wei Zheng,Jian Yu,Lei-Ying Ying,Bao-Ping Zhang 한국물리학회 2016 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.68 No.11

We demonstrated vertical-structured InGaN/GaN multiple-quantum-well (MQW) solar cells with enhanced performances at a wavelength of 510 nm. The enhancement was achieved by using a ptype ohmic mirror with a combined indium-tin-oxide film and an aluminum (Al) reflector inserted beneath the MQW absorption region. In addition, both good ohmic contact and high reflection were observed. The vertical-structured MQW solar cell with an Al reflector exhibited significant improvements in device performances as compared to that without the Al reflector, including a 49% increase in the short-circuit current density and a 56% increase in the power conversion efficiency.

Genetic and phylogenetic analysis of the honey bee sacbrood virus from jiangxi isolates

Meng Yaping,Yu Xinyue,Huang Qiang,Zhang Li Zhen,Wu Xiaobo,Wang Zilong,Yan Wei Yu 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1

The high prevalence of honeybee viral diseases poses a severe threat to the health of honeybees and causes substantial economic losses worldwide. Sacbrood virus (SBV) is a single-strand RNA virus that infects honeybees at all life stages. The infection can shorten the lifespan of adult bees and is lethal to larvae. SBV is the major cause of honeybee losses in Asia. Genetic and phylogenetic analyses of SBV isolates from different areas have been previously conducted. However, the impact of Apis mellifera Linnaeus and Apis cerana Fabricius coexistence on the infection and phylogeny of SBV remains unknown. In this study, we collected A. cerana and A. mellifera samples from commercial apiaries, only A. cerana in mountainous region. SBV prevalence was evaluated in three commercial apiaries of Jinxi, Tonggu and Nanchang and two mountainous regions of Zixi and Yifeng. In our sampling location, we found a higher SBV prevalence in the mountainous regions than in commercial apiaries. Partial structural polyprotein coding sequences were sequenced and compared with other GenBank SBV isolates. Phylogenetic tree topologies showed that SBV isolates form two major groups based on their host specificity, and isolates from same country tend to cluster together in subclades, indicating that the host and geographic region has significant effects on SBV strain specificity.

CASE REPORT : Crohn`s Disease Complicated with Duodenocolic Fistula: A Case Report

( Meng Tzu Weng ),( Shu Chen Wei ),( Yu Wen Tien ),( I Lun Shih ),( Jau Min Wong ) 대한장연구학회 2013 Intestinal Research Vol.11 No.4

Fistula formation is common during the course of Crohn`s disease, whereas duodenocolic fistulas are very rare. The management of internal fistulas in Crohn`s disease is a complex issue. Herein, we report a case of duodenocolic fistula manifested by increasing frequency of diarrhea and loss of body weight. The fistula was diagnosed by upper gastrointestinal tract barium series, magnetic resonance enterography, and panendoscopy and was treated with a right hemicolectomy and Whipple procedure because of the simultaneous occurrence of pancreatic head tumor. Subsequent treatment with adalimumab, azathioprine, and mesalazine was prescribed for the maintenance of disease remission, and the patient was well until 18 months after the surgery. (Intest Res 2013;11:299-302)

Collapse-resistant performance of a single-story frame assembly and multi-story sub-frame under an internal column-removal scenario

Wei-hui Zhong,Zheng Tan,Li-Min Tian,Bao Meng,Yu-hui Zheng,Shi-chao Daun 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.41 No.5

To elucidate the differences in the collapse behavior between a single-story beam-column assembly and multi-story frame, two 1/3-scale two-bay composite frames, including a single-story composite beam–column assembly and a three-story composite sub-frame, were designed and quasi-statically tested. The load–displacement responses, failure modes, and internal force development of the two frames were analyzed and compared in detail. Furthermore, the resistance mechanisms of the two specimens were explored, and the respective contributions of different load-resisting mechanisms to the total resistances were quantitatively separated to gain deeper insights. The experimental tests indicated that Vierendeel action was present in the two-dimensional multi-story frames, which led to an uneven internal force distribution among the three stories. The collapse resistance of TSDWA-3S in the flexural stage was not significantly increased by the structural redundancy provided by the additional story, as compared to that of TSDWA-1S. Although the development of the load response was similar in the two specimens at flexural stage, the collapse mechanisms of the multi-story composite frame were much more complicated than those of the single-story beam–column assembly, and the combined action between stories was critical in determining the internal force redistribution and rebalancing of the remaining structure.

PM2.5 in poultry houses synergizes with Pseudomonas aeruginosa to aggravate lung inflammation in mice through the NF-κB pathway

Meng Li,Xiuli Wei,Youzhi Li,Tao Feng,Linlin Jiang,Hongwei Zhu,Xin Yu,Jinxiu Tang,Guozhong Chen,Jianlong Zhang,Xingxiao Zhang 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.3

Background: High concentrations of particulate matter less than 2.5 µm in diameter (PM2.5) in poultry houses is an important cause of respiratory disease in animals and humans. Pseudomonas aeruginosa is an opportunistic pathogen that can induce severe respiratory disease in animals under stress or with abnormal immune functions. When excessively high concentrations of PM2.5 in poultry houses damage the respiratory system and impair host immunity, secondary infections with P. aeruginosa can occur and produce a more intense inflammatory response, resulting in more severe lung injury. Objectives: In this study, we focused on the synergistic induction of inflammatory injury in the respiratory system and the related molecular mechanisms induced by PM2.5 and P. aeruginosa in poultry houses. Methods: High-throughput 16S rDNA sequence analysis was used for characterizing the bacterial diversity and relative abundance of the PM2.5 samples, and the effects of PM2.5 and P. aeruginosa stimulation on inflammation were detected by in vitro and in vivo. Results: Sequencing results indicated that the PM2.5 in poultry houses contained a high abundance of potentially pathogenic genera, such as Pseudomonas (2.94%). The lung tissues of mice had more significant pathological damage when co-stimulated by PM2.5 and P. aeruginosa, and it can increase the expression levels of interleukin (IL)-6, IL-8, and tumor necrosis factor-α through nuclear factor (NF)-κB pathway in vivo and in vitro. Conclusions: The results confirmed that poultry house PM2.5 in combination with P. aeruginosa could aggravate the inflammatory response and cause more severe respiratory system injuries through a process closely related to the activation of the NF-κB pathway.

Diaporthe taoicola and D. siamensis, Two New Records on Citrus sinensis in China

( Meng Jiao Cui ),( Xin Wei ),( Peng Liang Xia ),( Ji Ping Yi ),( Zhi He Yu ),( Jian Xin Deng ),( Qi Li Li ) 한국균학회 2021 Mycobiology Vol.49 No.3

Two Diaporthe species isolated from fruit of Citrus sinensis in China were characterized based on morphology and multilocus phylogeny of ITS, tef1, and tub2 gene sequences. The phylogeny indicated that the two species match Diaporthe taoicola and D. siamensis. A critical examination of phenotypic characteristics confirmed the phylogenetic results. Diaporthe taoicola was morphologically characterized by producing Alpha conidia with tapering toward both ends. Meanwhile, D. siamensis produced cylindrical or ellipsoidal Alpha conidia with two oil drops. Pathogenicity tests revealed that both species were pathogenic to fruit of C. sinensis. To our knowledge, the two species were firstly reported on Citrus sinensis in China.

중국 콜드체인물류 운영서비스품질이 고객충성도에 미치는 영향 : 고객만족도 매개효과를 중심으로

여위(Yu, Wei),장몽택(Zhang, Meng-Ze),배기형(Bae, Ki-Hyung) 한국물류학회 2021 물류학회지 Vol.31 No.6

고속발전 중인 중국 콜드체인물류는 한⋅중 물류업계뿐만 아니라 양국 학자의 주목을 받고 있다. 기존한 연구에는 주로 중국콜드체인 물류기업운영이나 산업발전 측면에서 분석을 많이 하는데 소비자를 중심으로 고객충성도에 영향을 미치는 요인과 영향경로에 대한 연구가 아직 부족하다. 본 연구에서는 선행연구를 바탕으로 콜드체인물류의 서비스품질과 고객충성도의 관계 및 고객만족도의 매개효과에 대해서 확인해 보고 이에 따른 시사점을 제시하고자 한다. 이러한 연구 목적을 달성하기 위하여 본 연구는 중국에 307명 콜드체인물류서비스 경험자를 대상으로 연구 가설을 설정하고 SPSS 24.0과 Process Macro를 통하여 통계분석과 가설검정을 실행하였다. 가설검정 결과에 따라 다음과 같은 시사점을 도출할 수 있다. 첫째, 소비자 측면에서 보면 콜드체인 물류와 전통물류가 차이가 없어 운영물류 서비스품질의 개선은 고객 만족도를 높일 수 있는 좋은 수단이다. 따라서 콜드체인물류 기업은 고객 중심으로 좋은 품질의 서비스를 제공하여야 한다. 둘째, 콜드체인 물류의 운영물류 서비스품질에 대한 소비자 좋은 체험이 늘어나면 고객 만족도뿐만 아니라 고객 충성도도 높아질 수 있다. 따라서 기업 경영자는 제품 배송, 제품 포장, 파손율 감소 및 적시 배송 등 다양한 차원에서 콜드체인물류의 운영물류 서비스품질을 제고해야 한다. 셋째, 물류서비스에 대한 기대를 충족시키면 소비자가 같은 물류 서비스를 재사용할 의도뿐만 아니라 친구와 주변 사람에게 이 물류 서비스를 긍정하게 평가하거나 추천할 의도도 보인다. 따라서 고객만족도를 향상시키는 것은 기업 이미지 개선과 홍보확대에 도움이 되는 좋은 방법이다. 넷째, 고객만족도가 운영 물류 서비스품질과 고객충성도 간에 유의한 매개역할을 함으로서 콜드체인 물류기업은 정기적인 전화 답방과 만족도 조사 등 방법을 통하여 고객의 만족도 수준을 파악해야 한다. 본 연구의 이론적인 기여점은 실증연구를 통하여 고객충성도에 영향을 미치는 요인과 영향의 경로를 밝히고 중국 콜드체인물류 연구영역의 공백을 보완하는 것이다. 본 연구의 실무적인 기여점은 실증연구 결과를 바탕으로 시사점을 제시함으로서 향후 한국 콜드체인물류기업이 중국 소비자 성격을 파악하고 중국 시장에 진출할 때 유용한 참고자료가 될 것이다. China’s fast-growing cold-chain logistics is attracting not only the Korean and Chinese logistics industries but also scholars from both countries. Existing research is mainly conducted in terms of the operation of Chinese cold-chain logistics companies or industrial development, but consumer-centered research on factors and pathways that affect customer loyalty is insufficient. Based on existing research, this study identifies the relationship between service quality and customer loyalty of cold chain logistics and the mediating effect of customer satisfaction and presented suggestions. To achieve this research purpose, this study established research hypotheses for 307 cold-chain logistics service experiencers in China and conducted statistical analysis and hypothesis tests through SPSS 24.0 and Process Macro. Based on the hypothesis test results, the following implications can be derived: first of all, from a consumer’s point of view, there is no difference between cold chain logistics and traditional logistics, so improving the quality of operational logistics services is a good means to increase customer satisfaction. Therefore, cold chain logistics companies should provide good quality services centered on customers. Second, if increasing consumer’s experience of cold chain logistics operating logistics service quality, customer satisfaction as well as customer loyalty can also be increased. Therefore, corporate managers should improve the quality of operating logistics services for cold chain logistics in various dimensions, such as product delivery, product packaging, reduction of damage rate, and timely delivery. Third, if expectations for logistics services are met, consumers not only intend to reuse the same logistics service, but also to positively evaluate or recommend this logistics service to friends and people around them. Therefore, improving customer satisfaction is a good way to improve corporate image and expand publicity. Fourth, since customer satisfaction plays a significant mediating role between operational logistics service quality and customer loyalty, cold chain logistics companies should grasp the level of customer satisfaction through regular phone visits and satisfaction surveys. The theoretical contribution of this study is to clarify the factors and paths of influence that affect customer loyalty through empirical research and to supplement the gap in China’s cold chain logistics research area. The practical contribution of this study is based on the results of empirical research, which will be a useful reference for Korean cold chain logistics companies to grasp the characteristics of Chinese consumers and enter the Chinese market in the future.