Genetic and phylogenetic analysis of the honey bee sacbrood virus from jiangxi isolates

Meng Yaping,Yu Xinyue,Huang Qiang,Zhang Li Zhen,Wu Xiaobo,Wang Zilong,Yan Wei Yu 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1

The high prevalence of honeybee viral diseases poses a severe threat to the health of honeybees and causes substantial economic losses worldwide. Sacbrood virus (SBV) is a single-strand RNA virus that infects honeybees at all life stages. The infection can shorten the lifespan of adult bees and is lethal to larvae. SBV is the major cause of honeybee losses in Asia. Genetic and phylogenetic analyses of SBV isolates from different areas have been previously conducted. However, the impact of Apis mellifera Linnaeus and Apis cerana Fabricius coexistence on the infection and phylogeny of SBV remains unknown. In this study, we collected A. cerana and A. mellifera samples from commercial apiaries, only A. cerana in mountainous region. SBV prevalence was evaluated in three commercial apiaries of Jinxi, Tonggu and Nanchang and two mountainous regions of Zixi and Yifeng. In our sampling location, we found a higher SBV prevalence in the mountainous regions than in commercial apiaries. Partial structural polyprotein coding sequences were sequenced and compared with other GenBank SBV isolates. Phylogenetic tree topologies showed that SBV isolates form two major groups based on their host specificity, and isolates from same country tend to cluster together in subclades, indicating that the host and geographic region has significant effects on SBV strain specificity.

Upregulated lncRNA Gm2044 inhibits male germ cell development by acting as miR-202 host gene

Meng Liang,Ke Hu,Chaofan He,Jinzhao Zhou,Yaping Liao 한국통합생물학회 2019 Animal cells and systems Vol.23 No.2

Long non-coding RNAs (lncRNAs) have been found to participate in the regulation of human spermatogenic cell development. However, little is known about the abnormal expression of lncRNAs associated with spermatogenic failure and their molecular mechanisms. Using lncRNA microarray of testicular tissue for male infertility and bioinformatics methods, we identified the relatively conserved lncRNA Gm2044 which may play important roles in non-obstructive azoospermia. The UCSC Genome Browser showed that lncRNA Gm2044 is the miR-202 host gene. This study revealed that lncRNA Gm2044 and miR-202 were significantly increased in nonobstructive azoospermia of spermatogonial arrest. The mRNA and protein levels of Rbfox2, a known direct target gene of miR-202, were regulated by lncRNA Gm2044. Furthermore, the miR- 202-Rbfox2 signalling pathway was shown to mediate the suppressive effects of lncRNA Gm2044 on the proliferation of human testicular embryonic carcinoma cells. Understanding of the molecular signalling pathways for lncRNA-regulated spermatogenesis will provide new clues into the pathogenesis and treatment of patients with male infertility.

Design of Dynamic Calf Weighing System Based on Moving-IIR Filter Algorithm

Meng Hewei,Luo Jinjun,Li Shenglin,Qi Jiangtao,Wenwen Zhao,Li Yaping 대한전기학회 2021 Journal of Electrical Engineering & Technology Vol.16 No.2

In order to solve the problems of high intensity, low collecting effi ciency, poor information real-time performance and low precision in the calf weighing process, a kind of dynamic calf weighing system was designed. It was based on moving-IIR by establishing a test platform to verify fi lter algorithm. The software MATLAB was applied to design the moving average fi lter algorithm, IIR fi lter algorithm and moving-IIR fi lter algorithm, respectively, to process and analyze the dynamic data collected in slow, violent and slow-violent states of calves. Test results showed that, the error rates of moving-IIR fi lter algorithm in slow, violent and slow-violent states of calves were within 1.12%, 0.32% and 2.82%, which were lower than that of moving-average fi lter algorithm and IIR fi lter algorithm. In slow state of calves, the moving-IIR fi lter was not very smooth. In violent and slow-violent states of calves, the standard deviations were within 1.1126 and 1.1520, showing signifi cant smoothness. The study showed that, information collection system based on moving-IIR fi lter algorithm has fully taken the stability of moving fi lter and dynamic nature of IIR fi lter into consideration and had advantages of low error rate and high stability. Therefore, it can realize real time precise collection, display, storage and historical data query of weight information.

LncRNA Gm2044 highly expresses in spermatocyte and inhibits Utf1 translation by interacting with Utf1 mRNA

Ke Hu,Leina Li,Yaping Liao,Meng Liang 한국유전학회 2018 Genes & Genomics Vol.40 No.7

Spermatogenesis is a process which includes the following phases: spermatogonial stem cell proliferation and differentiation, spermatogonia, spermatocyte, spermatid and mature sperm. Spermatogenic failure is the important factor resulting in male infertility. Recent studies showed that long noncoding RNA (lncRNA) have been found to be involved in the regulation of male reproduction. However, lncRNA associated with spermatogenesis and their mechanisms of action are unclear. The aim of this study is to explore the role and molecular mechanism of lncRNA in spermatogenesis. LncRNA microarray of germ cells and bioinformatic analysis showed lncRNA Gm2044 may play potential roles in spermatogenesis. The expression level of RNA and protein were analyzed by RT-qPCR and western blotting, respectively. The interaction of lncRNA with mRNA was detected by RNA pull down and cellular proliferation was measured using CCK-8 reagent. Testis-enriched lncRNA Gm2044 is abundant in mouse spermatocytes. Gm2044 can suppress the translation of adjacent spermatogenesisrelated gene Utf1 by interacting with Utf1 mRNA. Furthermore, the proliferation of mouse spermatogonia GC-1 cell line and spermatocyte GC-2 cell line was inhibited by Gm2044. CONCLUSION: LncRNA Gm2044 was identified to inhibit Utf1 mRNA translation and play important roles in spermatogenesis.

Variations of microbial community in Aconitum carmichaeli Debx. rhizosphere soilin a short-term continuous cropping system

Fei Xia,Lina Wang,Jiayang Chen,Meng Fu,Guodong Wang,Yaping Yan,Langjun Cui 한국미생물학회 2021 The journal of microbiology Vol.59 No.5

Aconitum carmichaeli Debx. (Ranunculaceae) is a potential source of an important herbal drug named “Fuzi”, which is derived from the lateral root of the plant. Increased therapeutic usage resulted in the great demand for artificial cultivation of A. carmichaeli, however, the obstacles caused by continuous cropping is a serious problem. Continuous cropping has shown to affect the soil biological and non-biological factors. The current study attempted to discover the variations of microbial communities and soil properties in shortterm continuous cropping of A. carmichaeli. An experimental procedure with A. carmichaeli planted two years continuously was established. The variation of the soil microbial community, disease incidence, soil properties, and the correlation between soil microbe and disease incidence were investigated. The disease incidence increased during the continuous cropping of A. carmichaeli. The PCoA and LefSe results indicated that fungal communities in rhizosphere soil were altered during the short-term continuous croppingand the bacterial community was disturbed by the cultivation of A. carmichaeli, however, in the following two years of continuous cropping period, the soil bacterial community has not changed obviously. Proportions of some fungal and bacterial genera were varied significantly (p < 0.05), and some genera of microflora showed a significant correlation with adisease incidence of A. carmichaeli. Microorganisms contributing to community composition discrepancy were also elucidated. Continuous cropping of A. carmichaeli disturbed the rhizosphere soil microbial community and altered the soil chemical parameters and soil pH. These variations in soil may be related to the occurrence of plant diseases. The current study will not only provide theoretical and experimental evidence for the A. carmichaeli continuous cropping obstacles but will also contribute to A. carmichaeli agricultural production and soil improvement.