미치광이풀 모상근 배양에서 Tropane Alkaloid 생산성 증진을 위한 최적 생물학적 엘리시터 선발

정희영,강승미,강영민,김용덕,양재경,정영관,최명석 Plant molecular biology and biotechnology research 2003 Plant molecular biology and biotechnology research Vol.2003 No.-

Scopolamine and hyoscyamine which belong to tropane alkaloids are the pharmaceutically valuable anticholinergic drugs. In order to increase the productivities, the effects of elicitation were investigated during hairy root cultures of Scopolia parviflora. Biotic elicitors originated from 3 fungi and 1 yeast were prepared as homogenate and supernatant and added to 3-week-old cultures. Both of homogenate and supernatant of Candida albicans elicitors increased the scopolamine production. The production of hyoscyamine was enhanced by homogenate of Fusarium solani and supematant of C. albicans. Most of the other fungal elicitors were also improved on the tropane alkaloid production compared to non-treatment. Among the elicitors tested, C. albicans was proved the optimal biotic elicitor on tropane alkaloids production. These results will be served mass production of tropane alkaloids by large-scale production.

智異山 갓대의 形質에 關한 考察

鄭永觀,李康寧 진주농과대학 1970 진주농과대학 연구논문집 Vol.- No.9

갓대 開發에 있어서 基礎的인 方法의 一環으로 標高에 따른 갓대잎을 材料로 하여 그 形質 相互間의 相關關係및 遺傳力을 調査하였든바 그 結果를 要約하면 다음과 같다. 1. 葉長은 標高가 높을수록 길어지고 葉幅은 標高700m의 것이 가장 넓고 다음이 500m, 900m의 順으로 되고 葉幅이 넓어 짐에 따라 엽초장이 길어지는 傾向을 보였다. 2. 葉脈數는 標高 700m의 것이 가장 많고 다음이 900m, 500m의 順이고 葉脈數가 많아 짐에 따라 葉耳長이 길어지는 傾向을 보였다. 3. 標高에 따라 分散分析結果 葉長, 葉幅, 葉脈數, 엽초장, 葉耳長等 全形質間에 높은 有意性이 있었다. 4. 遺傳力은 葉長, 엽초장, 葉脈數, 葉耳長, 葉幅順으로 보였으나 그中 葉幅의 遺傳力은 比較的 낮게 보였다. 5. 葉長과 葉幅 間에는 相關關係가 없으며 葉脈數, 엽초장 間에는 높은 相關關係를 보였고 葉耳長 間에는 낮은 相關을 보였다. 6. 葉幅과 葉脈數間에는 相關關係가 없고 엽초장, 葉耳長 間에는 낮은 相關을 보였다. 7. 脈數葉와 엽초장 間에는 높은 相關關係를 보였고 葉耳長 間에는 낮은 相關을 보였다. 8. 葉초長과 葉耳長 間에는 낮은 相關關係를 보였다. 以上의 結果에서 標高 700m가 生育適地로 推定되고 遺傳力이 낮은 葉幅에 育林技術을 加한다면 數量增加에 效果가 있을 것으로 推定된다. The purpose of this report is to infer the favourable-site for Sasmorpha chiisanensis. For this purpose, correlations and heritabilities among characters of Sasamorpha chiisanensis based on the above sea levels are investigated. The character includes leaf length, leaf width, number of vein. length of leaf sheath and length of leaf ears. The results can be summarized as follows: 1. The averages, standard deviations, standard errors, coefficients of variation for the characters noted above are shown in table 1. according to which leaf length is appeared the longest at 500m of the above sea level, and average values of leaf width, number of vein, length of leaf sheat and length of leaf ears are maximum at 700m of the above sea level. 2. According to table 2(analysis of variance), the hypothesis that the mean of a character among the above sea levels be equal each other is rejected at l percent of significant level for all characters. 3. The magnitude of the heritability of each character, as shown table 3, is the leaf length, length of leaf sheath, number of vein, length of leaf ears, leaf width in order. That of leaf width is appeared comparatively low. 4. High correlations are found between leaf length and number of vein, leaf length and length of leaf sheath, leaf width and length of leaf sheath, leaf width and length of leaf ears and number of vein and length of leaf sheath. 5. And coefficients of correlation between leaf length and length of leaf ears, and number of vein and length of leaf ears are comparatively low. 6. But no relationships is found between leaf length and leaf width, and number of vein and leaf width. We might conclude, from above analysis, that the favourable-site for Sasamorpha chiisanensis can be said to be around 700m of the above sea level, and when the fact that heritability of the leaf width is significantly low as compared to others in taken into account, an advance in silvicultural technology might be considered to significantly increase production of Sasamorpha chiisanensis.

山地利用面에서 본 墓地制度 改善方案

孫英模,麻鎬燮,鄭永觀 慶尙大學校 1990 論文集 Vol.29 No.1

The demand and supply of land are to be steadily increasing as the population is mounting and the social, economic and indusrial development gradually grows. Therefore the effcient use of land becomes one of the most important issues for the national development and social welfare. Our country composed of forests with 65%, this potential value brings us unbonded benefit. The use of land for the graveyard, however, has suggested the very important social problem althought it occupies 2% of the forest land area. In view of this situation this paper was attempted to suggest the improvements for the issues of grave system in Korea. The improvements are following: 1. Reformation of national sene 2. Reduction of large scale of graveyard. 3. Urgent recovery of the effectiveness of the provision of the law which proved a dead letter. 4. The establishment fo park cemetery, and the amendment and supplements of the standard for the facilities. 5. Expulsion of a private graveyard of natural men. 6. Expansion of buring and cremation establishment. In conclusion ,the contents of kind of reform measures have the contents of being able to be practiced at once . and the contents of which requires the establishment of long term planning and practice also exist. Such things are complexly formed and we have to practice them with the continuous interest. and thus the relation between the living person and the dead person is not concerned to the loss which is given to the living person one sidely. but the living person and dead person hereby coexit.

葉成分과 土壤의 理化學的性質이 林木生長에 미치는 영향

李光洙,鄭永觀,朴南昌,崔載彩,姜眞擇 경상대학교 농과대학 부속연습림 2001 演習林硏究報告 Vol.- No.11

This study was carried out to analyze physical and chemical properties of soil and leaf analysis to estimating amount of tree growth in Chamaecyparis pisifera, Jinhae city, Gyeongnam province. The results were summarize as follows; As the results from analysis of relation of physical-chemical properties of soil and leaf component, the relationship p component inside soil and n component inside leaf showed positive correlation with determination coefficients(R^2=0.9986), and also relation of nitrogen component inside soil and component of Na_2O inside leaf showed positive correlation with determination coefficients(R^2=0.9987), but the relation of component Ca^2+ inside soil and component of Na_2O inside leaf showed negative correlation with determination coefficients(R^2=-0.9984), and also relation of p component inside soil and Fe component inside leaf showed negative correlation with determination coefficients(R^2=-0.7215). The results from analysis of relation of leaf component and tree growth, showed correlation of determination coefficient(R^2=0.4256) in height growth, determination coefficient (R^2=0.6248) in DBH growth, determination coefficient(R^2=0.6248,) in volume growth, respectively. In each tree growth of recent five years, N and P components showed positive correlation of determination coefficient(R^2=0.6170, 0.5780, respectively) in height growth, and also N and Na^+ components showed positive correlation of determination coefficient(R^2=0.6016, R^2=0.8727, respectively) in DBH growth, but K= component showed negative correlation in DBH growth As the results from analysis of stepwise regression to estimating amount of DBH growth, sequence of inputted variables entered in order of N, Na+, Mg++, ,and estimated equation was Y=44.791N-56.640Mg+0.6867Na-26.47, determination coefficients of 69%.

유지 투석중인 만성 신부전환자에서 허혈성 심질환의진단 지표로서의 심장트로닌-I의 유용성

신병철,강대웅,정지용,류봉관,서영욱,김정인,김범윤,김현리,정종훈 朝鮮大學校 附設 醫學硏究所 2004 The Medical Journal of Chosun University Vol.29 No.1

Background : Coronary disease is highly prevalent in patient with end stage renal disease (ESRD) and account for much of their observed morbidity and mortality. Troponin-I consistently maintains a high sensitivity and specificity and is most sensitive marker for ischemic heart disease (IHD). Method : We examed 49 hernodialyzed patients (22 male, 27 female) without evidence of acute coronary syndrome (ACS) for 6 months. Biochemical markers were measured in serial predialysis blood samples. For analysis, we used two cardio-specific assays for troponin-T (cTnT) as well as for troponin-I (cTnI) and compared the results with CK-MB (reference value ≤ 4.0 ng/mL) concentration. Results : Myocardial ischemia was observed in 47% (23/49) of patients. cTnT level above 0.1 ng/mL. and cTnI level above 0.5 ng/mL, were observed in 22% (11/49) and 20% (10/49) of patients respectively. cTnI revealed significantly higher positive rate in patients with myocardial ischemia than the patients without myocardial ischemia (43 % vs 30%) (p<0.05). cTnT and CK-MB revealed no difference in positive rate between the patients with and without myocardial ischemia (cTnT : 30% vs 15% and CK-MB : 30% vs 19%). The sensitivity and specificity of cTnI to myocardial ischemia were higher than those of cTnT and CK-MB (sensitivity 43% vs 30% and 30%. specificity 100% vs 85% and 81%). Conclusion: Both cTnT and cTnI are useful in ruling out myocardial injury in chronic renal failure patients. But. cTnI is a more sensitivity and excellent specificity of ischemic heart disease than cTnT and CK-MB in hemodialyzed patients. In patients with ischemic heart disease, the presences of DM and advanced age were higher than those in patients without ischemic heart disease (p<0.05). Among the baseline characteristics old age, elevated LDH and diabeties were significant more frequent in the patients with elevation of cardiac troponin-I (p>0.5 ng/mL) than those with cardiac troponin-I (p<0.5 ng/mL), p=0.038, p=0.049, and p=0.045, respectively. Our results suggest that these cTnI is the potential diagnostic marker for the prediction of IHD in ESRD patients.

혈소판 풍부혈장의 임상적 응용

하정완,김수관,조세인,이철우,정태영,김수흥,김영균 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.5

Platelet-rich plasma is an autologous source of platelet-derived growth factor and transforming growth factor beta that is obtained by sequestering and concentrating platelets by gradient density centrifugation. We have used platelet-rich plasma for bone graft, especially allobone graft, at implant surgery, sinus lift procedure, and cyst enucleation. This article is retrospective study from October 1999 to November 2000. All cases were good healing and clinical success.

山林의 公益的 機能 平價에 관한 考察

麻鎬燮,孫英模,金義庚,鄭永觀 慶尙大學校 1990 論文集 Vol.29 No.1

This study was carried out to estimate and evaluate the public function of forest resoruces, such as soil runoff control, landslide control, head-wsater conservation. purification of air (O? production and CO? purification ), sanitary andoutdoor recreation andwildlife protection for the forest land of 6.500.000 ha in Korea. The results obtaiend were as follows; 1 The function of soil runoff control was estimated to b e1.970million m?.its monetary value was appraised at 3.814.5 billion wons. 2.The function of landslide control was estimated to be 37.876 thousand m?its monetary value was appraised at 73.3 billion wons. 3. The function of head-water conservation was estimated to be 25.507million tons, its monetary value was appraised at 3.571.0 billion wons. 4.The production fo O? in the forest land was estimated to be 78.600 thousand tons,its monetary value was appraised at 3.930.0billion wons. 5.The volume of CO? absorbed in the forest land was estimated to be 94.320 thousand tons,its monetary value appraised at 2.204.3 billion wons. 6.The monetary value of outdoor recreation was appraised at 1.162.5 billion wons. 7.The phytoncide emmited in the forest land was estimated to be 1.954 thousand tons,the function of forest bath using phytoncide was appraised at 1.953.9 billion wons. 8.The monetary value of the functions of wildife protection, control of harmful insect control of tree growth and loss control of timber qality was appraised at 327 1 billion wons. In conclusion, the value of public functions of forest is more 23 times than that of the econmic function. production value of forest products, and comes to 14% of GNP. And also, the function of air purification.and and soil conservation has value than those of others. Particulary, it takes a more great deal of effort achiving the various works such as erosion control, establishment of recreation forest. management of reserved natural forest and afforestation of street tree for increasing of public functions.

H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

산청군 산림지가 형성요인에 관한 연구

권철현,이상태,신현철,최재채,정영관 경상대학교 농업생명과학연구원 2003 농업생명과학연구 Vol.37 No.1

본 연구는 산청군 남부와 북부지역 361개소의 공시지가와 일반시가에 대하여 분석하였다. 지가 형성요인에 대하여 단계별 회귀분석을 실시하였으며, 그 결과는 다음과 같다. 남부지역에 대한 공시지가와 관련된 5변수 모형에 의한 추정식은 Y = 332.1973 +0.0099X_26 - 0.3577 X_8+ 0.0291X_24 - 2.6768X_2 - 26.1452X_3 이며, 회귀식에 대한 설명력은 90%로 높게 나타났으며, 일반시가에 대한 추정식은 Y = 1035.7976 -185.8280X_21 -3.3937X_1 + 405.6658X_19 -0.4703X_8 이며, 추정식에 대한 설명력은 92%롤 비교적 높게 나타났다. 북부지역에 대한 지가 형성요인에 의한 공시지가 추정모형은 Y = 176.7875 + 0.0205X_25 -0.1168X_8 + 0.0015X_26 -46.3447X_21 로 추정되었으며, 회귀식에 대한 설명력은 96%, 일반시가의 추정 모형은 Y = 203.0697 + 0.0430X_25 + 0.0027X_26 -28.8276X_4 -46.7856X_21 -0.0007X_23 으로 추정되었으며, 회귀식에 대한 설명력은 90%로 나타났다. 이상과 같이 접근요인은 부의 영향을, 그 외 다른 요인들은 지가에 양의 영향을 미치는 것으로 나타났다. This study was carried out to analyze the publicly announced land price and actual land price compared to the northern and southern region of 361 forest land place in Sanchung county. To decipher factors for making forest land price it used the stepwise regression analysis. And the result of analysis are followings; Y = 332.1973 +0.0099X_26 - 0.3577 X_8+ 0.0291X_24 - 2.6768X_2 - 26.1452X_3 (R^2=90%) and the estimation equation of actual price analyze the publicly announced and actual price compared to the northern and southern regions of Sanchung county of price was obtained as Y = 1035.7976 -185.8280X_21 -3.3937X_1 + 405.6658X_19 -0.4703X_8(R^2=92%). In the northern region, the estimation equation of publicly announced land price by the factors for making forest land price was obtained Y = 176.7875 + 0.0205X_25 -0.1168X_8 + 0.0015X_26 -46.3447X_21(R^2=96%) and the estimation equation of actual price was obtained as Y = 203.0697 + 0.0430X_25 + 0.0027X_26 -28.8276X_4 -46.7856X_21 -0.0007X_23(R^2=90%). In the result of the stepwise regression analysis, it was a showed negative effect of the proximity factor and the positive effect between others factor and land price.

Enhanced CO₂ reduction activity of polyethylene glycol-modified Au nanoparticles prepared via liquid medium sputtering

Chung, Min Wook,Cha, In Young,Ha, Min Gwan,Na, Youngseung,Hwang, Jungsoo,Ham, Hyung Chul,Kim, Hyoung-Juhn,Henkensmeier, Dirk,Yoo, Sung Jong,Kim, Jin Young,Lee, So Young,Park, Hyun S.,Jang, Jong Hyun Elsevier 2018 Applied Catalysis B Vol.237 No.-

<P><B>Abstract</B></P> <P>The electrochemical conversion of CO<SUB>2</SUB> into useful chemicals such as CO is a promising strategy to reduce CO<SUB>2</SUB> emissions from fossil fuel consumption and to mitigate the impacts of global warming. Although tremendous effort has been devoted to the practical use of CO<SUB>2</SUB>conversion techniques, these techniques still suffer from deficient catalytic activity toward CO<SUB>2</SUB> reduction as well as a complex catalyst synthesis procedure. In this study, an effective strategy to enhance the catalytic CO<SUB>2</SUB> reduction activity with a unique synthesis method is proposed. Polyethylene glycol (PEG)-coated Au nanoparticles supported on a porous carbon support are prepared by a facile, cost-effective, and biocompatible one-step sputtering deposition method, termed liquid medium sputtering. The use of PEG as a liquid medium is advantageous in terms of catalytic activity and stability by producing PEG layers on the Au surface. The prepared PEG-coated Au nanoparticle catalyst exhibits a CO Faradaic efficiency of 100% at −0.57 V<SUB>RHE</SUB> and excellent stability during 10 h of operation due to the high solubility of PEG for CO<SUB>2</SUB>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Uniform Au nanoparticle synthesis via liquid medium sputtering of in polyethylene glycol solvent. </LI> <LI> Au nanoparticles layered with the polyethylene glycol displays enhanced CO<SUB>2</SUB> reduction activity. </LI> <LI> Polyethylene glycol coated Au exhibits improved stability for CO<SUB>2</SUB> reduction compared to bare Au. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Polyethylene glycol coated Au nanoparticles showed enhanced electrocatalytic activity toward CO<SUB>2</SUB> reduction reaction likely due to the enhanced CO<SUB>2</SUB> concentration at the catalytic surfaces.</P> <P>[DISPLAY OMISSION]</P>