Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122의 생산과 그 특성

최용석,옥승호,유주현,배동현 한국산업미생물학회 1997 한국미생물·생명공학회지 Vol.25 No.1

토양으로부터 분리한 Metarrhizium anisopliae (Metschn.) Sorok이 생산하는 biopolymer를 정제하여 분석하고, 그의 생산조건을 검토하였다. 탄소원으로 mannitol, 질소원으로는 polypepton을 사용했을 때 가장 많은 biopolymer를 생산하였고 K_2HPO_4 및 CaCl_2를 첨가함으로서 생산량이 더욱 증가하였다. 또한 이 biopolymer를 정제하여 그 분자량과 구성성분을 검토한 결과 Metarrhizium anisopliae(Metschn.) Sorok이 생산하는 Biopolymer YU-122는 분자량이 1.7×10 exp (6)이고, C, H의 구성비가 1:2이며 미량의 N이 검출되었으나, 주 구성성분은 glucose와 galactose의 환원당으로 이루어져있는 전혀 새로운 biopolymer인 것으로 나타났다. To produce biopolymer, Metarrhizium anisopliae (Metschn.) Sorok was cultured in a medium containing glucose 1.0%, sucrose 2.0%, soluble starch 1.0%, yeast extract 0.5%, polypeptone 0.5%, K_2HPO_4 0.1%, MgSO_4·7H_2O 0.02%. The culture broth was centrifuged and the polymer was harvested by adding methanol to the culture supernatant. When three times of methanol was added, the polymer was coagulated and precipitated. Then it was further purifued through successive SK-1B, SA-20P, HP-20 column chromatographies. This polymer was designated as Biopolymer YU-122. C:H ratio of this Biopolymer YU-122 was 1:2 and small amount of N is detected by CHN analyzer. Glucose and galactose are main components of this polymer. Average molecular weight of this biopolymer was 1.7×10 exp (6) by Sepharose 4B gel permeation chromatography. Optimal condition for biopolymer production was investigated. When 5% of mannitol was used as a carbon source, and polypepton as a N source, highest productivity of biopolymer was achieved. C/N ratio as nutrient was also a major factor in polymer production and its optimal ratio was 3.

바닥공조시스템과 천장공조시스템의 실내환경 비교 평가

유기형,조동우,유지용,이진영,이상엽 대한건축학회 2004 대한건축학회논문집 Vol.20 No.5

The purpose of this study was to evaluate the indoor environment of office building with underfloor air distribution system and ceiling based air conditioning system. The underfloor air distribution system was introduced to use access floor and aim to economical motive such as reducing floor to height level. Researches were not sufficient to preserve the characteristics between ceiling based air conditioning system and underfloor air distribution system. Experiments have been carried out to indoor environment in the summer and winter. As the result, thermal environment of the underfloor air distribution system is better than ceiling based air conditioning system in the winter. Ventilation effectiveness of the underfloor air distribution system is bigger than ceiling based air conditioning system.

전문비서란 무엇인가? : Its Concept and Function

김주연,용주현,신동희,유민,최정윤 이화여자대학교 법정대학 비서학과 1995 비서학연구 Vol.- No.15

회전익 항공기용 외활형식 착륙장치의 정ㆍ동적 분석에 관한 연구

유용석,이동명,채경덕,오택열 경희대학교 산학협력기술연구원 1996 산학협력기술연구논문집 Vol.2 No.-

It is important to make sure of design's reasonability concerning of structural vibration problems and safety with fulfilling the research and with analyzing the body structures that include the landing gear which has dynamic significant characteristics in helicopter, and to make systematical procedure which obtains engineering data to guarantee the quality of the helicopter that was developed by testing of parts, subsystems and body. In this study, Analysis of landing gear under FAR regulations was attempted, and systematical procedure for static and dynamic characteristics of the landing gear in many subsystems of the helicopter was classified.

마이크로 프레스 구동을 위한 기어시스템 개발

홍용,김현식,고유곤,홍동표,김성욱 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

In this paper, gear system for Micro-press actuator parts is developed. The micro-press system consists of AC geared servo motor, gear system and high precision ball screw. Herein, the micro-press gear system not only forms the micro thin foil valve but also forms the hydro dynamic bearing for a high precision rotational equipment, we analyzed the transmitted power capacity of gear system and torque, angular velocity of ball screw, because we are to select the appropriate servo motor and then we apply result of analysis to the micro press system. For the next step, we plan to study for the development of forming speed for a productivity with high precision.

부산지역 모 공단 및 인근지역 사업체의 산업보건 실태에 관한 조사연구

이동영,손혜숙,이채언,박형종,김대환,엄상화,최성용,유병철 인제대학교 1993 仁濟醫學 Vol.14 No.4

부산지역 공단 및 인근지역 사업체의 산업보건실태를 파악하기 위하여 205개 사업체의 보건담당자를 대상으로 1990년도의 보건실태에 관한 설문조사를 하였다. 조사내용을 일반사항, 보건관리실태에 관한 사항, 작업장 유해환경 및 보호구 지급사항, 건강관련 사항 및 근무조건에 관한 사항 등으로 대별하여 분석ㆍ관찰하였다. In order to obtain the fundamental data for developing more effective and acceptable programs of public health manegement in an industrial complex, 205 health manager in the plants answered the questionaire of the status of industrial health during 1990. The questionaire consisted of general charateristics, health care status, environmental problems, health problems, and others. 72.4% of 205 plants had less than 100 workers. Fabricated metal manufacture was 32.4%. Although Footwear manufacture was 6.4%, the percentage of workers was 33.1% of total workers. The percentage(56.2) of retired women was higher than that(38.2) of men. 51.9% of total plants has recieving group health care services. 85.4% of plants was exposed to noise, 66.5% was exposed to dust. Ear plug and mask were given to prevent noise and dust in 69.2% and 68.6%, respectively. Among 22,299 workers, 618(2.8%) were diagnosed as suspected non-occupatinal disease. 85.9% of them worked on continuously and had their disease treated. The cases suspected for occupational disease was 100 workers. 82.5% of them changed their post. 45% had the compensation for their occuaptional disease. The rate of working: accidents was 2.3%.

迎日灣의 ECOSYSTEM 硏究 3. 水溫

尹伊鏞,朴相來,李東澈,金基台,崔銀珠,安由煥 嶺南大 海洋科學硏究所 1988 Marine Nature Vol.1 No.1

In order to observe hydrological characteristics in the Gulf of Yoeng-il, measurements of water temperature have been carried out from February to August 1983, and from August 1985 to December 1986. Surface water temperatures are closely linked to air temperature and solar-radiation for all littoral stations. Seasonal and annual variations of all horizontal stations are very similar to one another. Vertical variations of water temperatures for the stations located along the central-line of the Gulf are characteristic (and observed) as thermoclines and thermal stratification in the hottest summer-time (August). It is interesting to note that water column temperature of July and September are rather warmer than August, on account of a cold water mass coming from NE direction. For example, surface temperature in June 1982 was above 19℃, but the water below a depth of about 30m drastically drops to 4℃. It is indicative of invasion of water mass from cold current or deep sea.

정신분열병과 Neuregulin 1 유전자의 연합연구

박동연,이유상,조은영,조승희,장용이,전현옥,장수연,윤세창,김종원,홍경수 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.3

Objectives : The authors recently found a suggestive evidence of linkage of chromosome 8p21-12 to schizophrenia in Korean multiplex families. Neuregutin 1 (NRGI) was identified in this locus as a positional and functional candidate gene for Schizo-phrenia, through several independent studies with European and Chinese populations. The purpose of this study is to determine whether NRGl is associated with schizophrenia in Korean population. Methods : Three SNPs (SNP8NRG221533, SNP8NRG241930, SNP8NRG243177) and two microsatellites markers (478B14-848,420M9-1395) located at the 5' end of NRGI were genotyped for 242 unrelated schizophrenia patients and the same num-ber of normal controls. Genetic association was tested by χ²-test (df=1). Not only for the whole patients group but also for asubgroup of patients with auditory hallucination. This subtype showed stronger linkage with chromosome 8p12 in the prior study of the authors with multiplex families. Results : G allele of SNP8NRG241930 was significantly in excess in the subgroup of patients with auditory hallucination compared to the control group (p=0.03, 0R=1.76). We also found that 3 SNPs haplotype TTC (p=0.04, 0R=0.58) and five markers haplotype TTC53 (p=0.01,0R=0.49) were associated with schziophrenia with a protective effect. Three SNPs haplotype CGT which is a part of the at-risk haplotype of the Icelandic schizophrenia families was found in excess in the patients group but no significant association was observed. Conclusion : NRGI might either play a mle in the predisposition to schizophrenia or be in linkage disequilibrium with a causal locus of this illness.

데이터마이닝의 태스크계획접근을 위한 지능형 사용자 인터페이스 설계

김민용,유동하 경희대학교 사회과학대학 1998 社會 科學 論叢 Vol.16 No.-

데스크탑 마이크로 성형시스템의 가진성형에 따른 동특성 연구

홍동표,고유곤,홍용,김현식,이재완 한국공작기계학회 2009 한국공작기계학회 춘계학술대회논문집 Vol.2009 No.-

In this study, a desktop forming system which enables hydro dynamic bearing forming was developed. It consists of power train system, frequency forming system, manufactured Die-set, and controller of desktop forming system. The desktop micro forming system is focused on a higher load capacity and a shorter forming time than conventional system. Therefore it was designed for power train capability and load condition of Ball Screw. Moreover, a Piezo actuator was installed to make accurate machining possible, however, the Piezo actuator generate noise and vibration. Thus we performed the analysis of dynamic characteristics through the experiment about developed system in order to reduce such problems and discuss the alternative methods.