황색 포도상구균의 vancomycin 내성 원인 유전자의 동정

전준연,양영기,송민동 조선대학교 부설 유전생물공학연구소 1991 유전생물공학연구지 Vol.1 No.1

임상 분리되는 Gram 양성균인 황색 포도상구균(Staphyhcoccus aureus)에 대한 각종 학생물질의 최소 발육저지농도 측정(Minimal Inhibitory Concentration: MIC test)과 내성 pattern을 조사하였으며, 이들 균주 중 특히 vancomycin에 대하여 높은 내성을 나타내는 균주(Vancomycin resistant Staphylococcus aureus: VRSA)에 대한 vancomycin 내성원인 유전자의 탐색 및 cloning을 실시하여 vancomycm 내성기구의 연구에 필요한 조사를 수행하였다. Vancomycm에 내성인 균주(VRSA)를 고온(43.5℃) 처리하여 계대 배양을 한 다음, replica plating method에 의 해 vancomycin 감수성 균주(Vancomycin-sensitive Staphylococcus aureus: VSSA)를 얻었으며, VRSA 균수와 VSSA 균주로부터 membrane protein을 준비한 다음 SDS-poly-acrylamide gel electrophoresis(SDS-PAGE)로 전기 영동하여 그 단백질 pattern을 비교한 결과, VRSA 균주에서는 분자량(Mr: relative molecular weight)이 약 127Kadl 그리고 30Kdal 정도 되는 부위에서 vancomycin 내성에 관계되는 듯한 특이적인 단백질을 관찰할 수 있었다. 그러나 VSSA 균주에서는 이러한 단백질을 관찰할 수 없었다. 한편 이들 균주로부터 plasmid DNA를 분리한 다음 agarose gel electrophoresis하여 그 pattern을 분석한 결과, VRSA 균주에서는 약50Kb부위에서 plasmid DNA밴드가 관찰되었으나 VSSA 균주에서는 아무런 밴드도 관찰되지 않았다. 이상의 결과로 볼때 vancomycin 내성유전자는 plasmid DNA상에 존재한다고 생각되어진다. This study was carried out to study the characterization of the gene that is responsible for the vancomycin-resistant Staphylococcus aureus (VRSA) In order to obtain vancomycin-sensitive S. aureus(VSSA)from VRSA stain, vancomycin-resistant S. aureus(VRSA) was subcultured twice at the high temp.(43.5℃)and then VSSA was obtained by replicaplating method after MIC test with vancomycin in high temp. treted VRSA strain. Membrane proteins were prepared from VRSA and VSSA strain and protein patterns of those were compared bny 7.5% SDS-polyacrylamide gel electrophoresis. Only in VRSA strain appeared novel protein bands at a position about 120 Kdal, 80 Kdal and 30Kda1, but it did not appear in VSSA strain. The patterns of plasmid DNA in VRSA and VSSA strain were clearly classified. Plasmid DNA band in VSSA strain appeared at a position about 50Kb, but it did not appear any band in VSSA strain. These results suggested that plasmid DNA may be encode the gene that is responsible for vancomycin-resistance.

충남대학교 학술림 산책로 훼손실태 및 관리방안에 관한 연구

이준우,김명준,최윤호,전용준,오도교,김민호,이소연,이경형 충남대학교 농업과학연구소 2004 농업과학연구 Vol.31 No.2

This study concentrates an the trail of the CNU(Chungnam National University) forest. The amount of use and the pattern of damage are considered, as well as the extent of environmental damage. Finally, some managerial strategies are proposed in connection with sustainable maintenance. The trail of the university forest is composed of three sections; one is the neighborhood park and the Institute of Information Technology/Communications, the second is the Institute of Information Technology/Communications and the Dormitory, and the third section is the Dormitory and the Foreign prof's Apt. The total length of the trail is 2.6km. The daily amount of use is 1,269 visitors per day. The amount of using increased three times the amount of that in 1998. This study shows the pattern of damage of the trail in the order of root-exposure, widening, rock-exposure, deepening and the divergence in the frequency of occurrence of all these factors. Environmental damage is also graded thus: grade 3 is 27.4m, grade 4 is 0.8m, grade 5 is 1.5m, and grade 6 is 0.2m. Accordingly, some managerial strategies were proposed in connection with the sustainable trail maintenance.

연속회분식 반응기를 이용한 질산화, 탈질 및 인의 제거에 관한 연구

정연규,정연균,전석준 연세대학교 산업기술연구소 1992 논문집 Vol.24 No.1

In this study nutrients treatment by sequencing batch reactor was performed. In doing so, nitrogen and phosphorus removal efficiencies were evaluated by changing SRT and mixing ratio for reaction each other, Besides nitrification, denitrification and phosphorus removal patterns were investigated through track studies on 1 cycle. As the results when operating mixing ration as 0∼0.8, maximum nitrogen removal efficiency was 87.6% at 0.4. While phosphorus removal efficiencies were more than 85.5% except operation condition of no mixing period. As SRT increased 5∼30 day, nitrogen removal efficiencies increased 70∼80%, which represented slight changes. But phosphorus removal efficiencies were 49.0∼97.3%, which represented sharply decreasing tendency less than 20 day. Also as the results of track studies on 1 cycle, ?? from aeration period was removed at the beginning of mixing period and then ?? was released from sludge in anaerobic mixing period followed by the luxury uptake in aeration period.

독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

Dosimetric evaluation of magnetic resonance imaging-guided adaptive radiation therapy in pancreatic cancer by extent of re-contouring of organs-at-risk

Jun Yeong Song(Jun Yeong Song),Eui Kyu Chie(Eui Kyu Chie),Seong-Hee Kang(Seong-Hee Kang),Yeon-Jun Jeon(Yeon-Jun Jeon),Yoon-Ah Ko(Yoon-Ah Ko),Dong-Yun Kim(Dong-Yun Kim),Hyun-Cheol Kang(Hyun-Cheol Kang) 대한방사선종양학회 2022 Radiation Oncology Journal Vol.40 No.4

Purpose: The safety of online contouring and planning for adaptive radiotherapy is unknown. This study aimed to evaluate the dosimetric difference of the organ-at-risk (OAR) according to the extent of contouring in stereotactic magnetic resonance image-guided adaptive RT (SMART) for pancreatic cancer. Materials and Methods: We reviewed the treatment plan data used for SMART in patients with pancreatic cancer. For the online contouring and planning, OARs within 2 cm from the planning target volume (PTV) in the craniocaudal direction were re-controlled daily at the attending physician's discretion. The entire OARs were re-contoured retrospectively for data analysis. We termed the two contouring methods the Rough OAR and the Full OAR, respectively. The proportion of dose constraint violation and other dosimetric parameters was analyzed. Results: Nineteen patients with 94 fractions of SMART were included in the analysis. The dose constraint was violated in 10.6% and 43.6% of the fractions in Rough OAR and Full OAR methods, respectively (p = 0.075). Patients with a large tumor, a short distance from gross tumor volume (GTV) to OAR, and a tumor in the body or tail were associated with more occult dose constraint violations—large tumor (p = 0.027), short distance from GTV to OAR (p = 0.061), tumor in body or tail (p = 0.054). No dose constraint violation occurred outside 2 cm from the PTV. Conclusion: More occult dose constraint violations can be found by the Full OAR method in patients with pancreatic cancer with some clinical factors in the online re-planning for SMART. Re-contouring all the OARs would be helpful to detect occult dose constraint violations in SMART planning. Since the dosimetric profile of SMART cannot be represented by a single fraction, patient selection for the Full OAR method should be weighted between the clinical usefulness and the time and workforce required.

Glucose detection via Ru-mediated catalytic reaction of glucose dehydrogenase

Jeon, Won-Yong,Choi, Young-Bong,Lee, Bo-Hee,Jo, Ho-Jin,Jeon, Soo-Yeon,Lee, Chang-Jun,Kim, Hyug-Han VBRI Press AB 2018 Advanced materials letters Vol.9 No.3

Clinically amyopathic dermatomyositis (초)

( Yeon Soo Kang ),( Woo Seok Jeon ),( Hyun Jong Lee ),( Hei Seung Kim ),( Hyung Ok Kim ),( Young Min Park ),( Jun Young Lee ) 대한피부과학회 2011 초록집 Vol.49 No.10

Case Report : Complete Atrioventricular Block-Induced Torsade de Pointes Manifested by Epilepsy

( Jun Han Jeon ),( Sung Ho Her ),( Jung Yeon Chin ),( Ki Hoon Park ),( Hee Jeong Yoon ),( Jong Min Lee ),( Seung Won Jin ) 대한내과학회 2011 The Korean Journal of Internal Medicine Vol.26 No.1

Complete atrioventricular (AV) block is frequently regarded as a cause of informed syncopal attacks, even though the escape rhythm is maintained. Torsade de pointes (TdP) may be a significant complication of AV block associated with QT prolongation. Here, we report the case of a 42-year-old female who was referred to our hospital due to recurrent seizure-like attacks while taking anti-convulsant drugs at a psychiatric hospital. TdP with a long QT interval (corrected QT = 0.591 seconds) was observed on an electrocardiogram (ECG) taken in the emergency department. The patient`s drug history revealed olanzapine as the suspicious agent. Even after the medication was stopped, however, the QT interval remained within an abnormal range and multiple episodes of TdP and related seizure-like symptoms were found via ECG monitoring. A permanent pacemaker was thus implanted, and the ventricular rate was set at over 80 beats/min. There was no recurrence of tachyarrhythmia or other symptoms. (Korean J Intern Med 2011;26:99-102)

Study on the Transient Electroluminescence of Phosphorescent OLEDs

Jun Ho Youn,Yeon Il Lee,문희태,Tae Jin Park,Woo Sik Jeon,권장혁,장진 한국물리학회 2009 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.55 No.3

We have studied the turn-off dynamics of phosphorescent organic light-emitting diodes (OLEDs) by investigating the temperature, pulse width, and voltage dependences of the radiative triplet lifetime with the dispersive parameter , calculated from the second luminescence of the transient electro-luminescence. This behavior is caused by the redistribution and recombination of electrons and holes accumulated near the interfaces. The lifetime increases with increasing carrier density and with decreasing temperature. This is due to the decrease in the hopping and the triplet-triplet annihilation rates. The experimental data satisfy the relationship between the dispersive parameter γ and temperature T, γ − 1 / exp(−A/T), with a constant A.

Swine Gut Microbiome and Malodor Compounds in Farms of South Korea

Yeon-Jae CHOI,Mirieom PARK,You-Rim SHIN,Jung-Ae KIM,Jeong-Sub SONG,Hyun-Jun JANG,Jinmo JEON,Dae-Hyuk KIM,Yangseon KIM 한국생물공학회 2020 한국생물공학회 학술대회 Vol.2020 No.10