개에서 17-ODYA가 신혈류량 자동 조절에 미치는 영향

양훈모,민영기,박중섭 순천향의학연구소 1999 Journal of Soonchunhyang Medical Science Vol.5 No.1

17-ODYA is a potent inhibitor of the formation of 20-hydroxyeicosatetraenoic acid, epoxyeicosatrienoic acids and dihydroeicosatetraenoic acids by renal cortical microsomes. This study characterized the effects of intrarenal infusion of 17-ODYA on renal blood flow (RBF) and autoregulation of RBF. Seven dogs were prepared for the measurements of RBF, mean systemic and renal perfusion pressure. The renal perfusion pressure was able to be adjusted to desired levels by an inflatable supra-aortic occluder. Intrarenal infusion of 17-ODYA produced a diuresis and a natriuresis but no change in RBF. 17-ODYA had no effect on autoregulation of RBF: Both the plateau and steep portrions. of the autoregulatory curves were identical to control. Thesholds for lower limit of autoregulation were 72±4 and 72±5 mmHg during control and 17-ODYA and not significantly different from each other. These results suggest that endogenous cytochrome P-450 metabolites of AA do not influence RBF autoregulatory capacity in dogs.

ER유체를 이용한 댐퍼의 설계 및 성능에 관한 연구

박성태,양헌석 울산대학교 2001 공학연구논문집 Vol.32 No.1

본 논문에서는 ER(electrorheological)유체의 동역학적 특성치를 실험적으로 구하고, 그 특성치와 빙햄 플라스틱 모델을 이용한 ER댐퍼의 해석 방법을 제시하였다. 또한 실린더형상의 전극을 가지는 Poiseuille 유동형 ER댐퍼에 대하여 평판 모델에 의한 근사적 해석과 실린더 모델의 해석결과를 비교하였다. 그리고 실제 Poiseuille유동형 ER댐퍼의 설계 및 제작으로 그 성능에 대한 고찰과 이러한 모델의 타당성을 검증하였다. 동시에 속도뿐만 아니라 전기장의 제어로도 넓은 범위의 감쇠력이 제어 가능함을 알 수 있었다. 따라서 본 연구를 통해 ER댐퍼의 성능에 대한 고찰과 제시된 해석 방법은 ER댐퍼의 초기 설계에 직접 응용될 수 있을 것이다. In this paper, the dynamic properties of ER(electrorheological) fluid and the analysis technique of ER damper using Bingham plastic model were presented. And approximation parallel plate model was introduced for Poiseuille flow ER dampers with cylindrical geometry. The approximation parallel plate model analysis compared with the cylindrical axisymmetric model analysis. To validate this analysis, the Poiseuille flow ER damper was designed, fabricated and tested. Also the result of test with ER damper showed that the damping force of ER damper was varied with the piston velocity and the intensity of electric field. Therefore the proposed analysis technique which is using Bingham plastic model can be used for initial development stage of ER damper. Key Words : Bingham plastic model, ER(electrorheological) fluid, Poiseuille mode

개정판 인지기능 장애 진단 도구 : 반구조화 인지기능 평가 도구

박종한,김희철,이양현,이종훈 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.3

연구목적 : 본 연구는 새로 개정된 CIDI의 신뢰도와 진단적 타당도를 평가하기 위해서 시행되었다. 방 법 : 대상은 어느 대학병원 정신과에서 진료받은 환자들 중에서 치매 환자 64명과 치매 이외의 다른 정신장애 환자들 중에서 60세 이상은 90명이었다. 그들에서 CIDI의 하위 검사 성적들간의 상관 관계, 내적일치도, 총점과 치매를 평가하는 다른 척도 성적들과의 상관 관계, 치매 진단의 민감도와 특이도를 이용한 진단 구분점의 선택, 그 구분점에서의 receiver operating characteristic 곡선을 이용한 area under curve측정 등이 시행되었다. 결 과 : CIDI하위 검사 성적들간의 Pearson 상관 계수는 0.596 내지 0.813이었고, 평균은 0.707이었고, 하위 검사 성적과 해당 하위 검사를 제외한 총점간의 상관 관계는 0.759 내지 0.890이었다. Cronbach α는 0.941이었고 어느 하위 검사를 삭제해도 별 영향을 받지 않았다. 치매 환자들에서 CIDI성적은 다른 척도를 성적과의 상관 관계가 0.5내외이었고, MMSEK총점과 CIDI 총점에서 MMSEK 총점을 뺀 점수와의 상관계수는 0.894이었다. CIDI의 area under curve는 0.933이었고 area under curve의 표준 오차는 0.021(95% CI, 0.891∼0.975)이었다. 치매를 진단을 위한 구분점은 65/66가 가장 적절할 것 같고, 이 구분점에서 민감도와 특이도는 각각 95.3%와 85.6%이었다. 그에 상응하는 MMSEK의 값들은 각각 0.930, 0.020(95% CI, 0.891∼0.969), 22/23, 89.1%와 85.6%이었다. CIDI와 MMSEK의 area under curve의 차이는 통계적으로 유의하지 않았다. CIDI의 총점은 물론 모든 하위 검사는 치매와 비치매 노인들에서 높은 수준의 감별 능력을 보여 주었다. 결 론 : CIDI는 각종 신뢰도와 각종 진단적 타당도가 만족할만한 수준이었다. 그리고 CIDI는 임상적 목적으로 인지기능을 평가할 때, MMSEK는 인지기능 장애의 유무에 대한 선별 검사 목적으로 사용되는 것이 바람직할 것이다. Objectives : This study was conducted in order to evaluate reliability and diagnostic validity of the CIDI. Methods : 64 patients with DSM-IV dementia and 90 elderly patients with other mental disorders entered this study. Inter-subtest Pearson's correlation, corrected subtest-total correlations and Cronbach's α were computed for reliability while correlation of the total scores on CIDI with the performances on other scales relating to dementia, sensitivity and specificity, area under curve using the receiver operating characteristic curve, and group comparison were used for the assessment of validity. Results : The CIDI's inter-subtest correlations ranged from 0.596 to 0.813 with a mean of 0.707. the corrected subtest-total correlations were between 0.759 and 0.890. Cronbach's α of the subtests was 0.941 and the Cronbach's αs were between 0.928 and 0.948 when any subtest was deleted. In the demented subjects, the correlation coefficients of the total CIDI score with the performances on S-SDQ, IADL, BADL, BDRS, and MMSEK were around 0.5 Area under the receiver operating curve of CIDI was 0.933 with the standard error of 0.021(95% CI, 0.891-0.975). At the CIDI cutoff point of 65/66, sensitivity and specificity were 95.3% and 85.6%, respectively. The corresponding values of MMSEK were 0.930, 0.020(95% CI, 0.891-0.969), 22/23, and 89.1% and 85.6%. The demented scored much poorer in all CIDI subtests than the nondemented mental patients, and the differences were highly significant. Conclusions : The CIDI was satisfactory regarding to reliabilities and diagnostic validities. The CIDI and MMSEK are thought to be reciprocal in assessment of cognitive function : CIDI is preferable for clinical assessment of cognitive dysfunction and MMSEK for as a screening test.

방사선 조사를 받은 흰쥐 신경뇌하수체 관문의 미세구조 : 관문의 새로운 개념설정 Establishment of a New Concept on the Barrier

이헌용,안의태,양남길,고정식,박경호 순천향의학연구소 1995 Journal of Soonchunhyang Medical Science Vol.1 No.2

신경뇌하수체는 뇌의 일부지만 뇌실둘레기관(circumventricular organ)으로서 전형적인 혈액-뇌관문이 없으므로 혈관 안팎의 물질이동이 자유로운 신경내분비기관이다. 이 실험에서는 심한 방사선 조사시에 혈액-신경뇌하수체관문의 형태적 대응을-연구함으로써, 관문의 특성을 분석했으며, 관문의 구성을 구체적으로 설정하여 이를 개념화 할 것을 제안하였다. 체중 200-250g의 숫흰쥐를 sodium thiopenthal 로 마취시킨 후 Mitsubishi선형가속기로 방사선 조사를 시켰다. 조사조건은 거리 80cm, 조사구역 30 X 30cm, 조사깊이 1.2cm, 조사속도 분당 200 rads로 하여 실험군에 따라 3,000 rads 또는 6,000 rads가 되도록 했다. 조사후 6시간, 2일, 6일후에 각군의 동물을 도살 하여 신경뇌하수체를 떼어냈다. 떼어낸 조직은 1% glutaradehyde- 1% paraformaldehyde액에 1차 고정하고, 1% osmium tetroxide액에 2차 고정하였고, araldine혼합액에 포매된 조직은 절편을 만들어 uranyl acetate와 lead citrate액으로 염색해서 전자현미경으로 관찰하였다. 주로 모세혈관주위공간에서 관문구조를 중심으로 관찰한 결과 3,000 rads 조사군과 6,000 rads조사군 모두가 비슷하게 심한 변화를 보였고, 변화의 정도가 2일군에서 가장 심했으며 6일군에서는 약간 안정되는 모습이었으나 변화의 양상은 모든 실험군을 통해서 비슷하였다. 가장 두드러진 변화는 모세혈관주위공간에서 나타났는데 공간의 확장, 큰포식세포의 증가와 활성화, 신경뇌하수체세포속에 들어 있던 신경종말들의 이탈, 먼지 같은 물질의 증가 등을 들 수 있었다. 특히 큰 포식세포의 변화는 매우 특징적이어서 넓은 세포질관(판상족, lamellipoda)을 형성하여 모세혈관과 신경뇌하수체세포사이를 차단시킬 뿐아니라 왕성한 포식작용으로 공간내의 축삭종말들을 용해시켰다. 큰포식세포의 세포질안에는 과립형질내세망들이 매우 발달하였고, 세포질 영역이 매우 넓어진 것으로 볼 때, 이들이 기동타격대로서 활발히 움직이는 매우 효율적인 관문의 구성성분으로 생각되었다. 특히 신경뇌하수체는 전형적인 혈액-뇌관문이 없으므로 신경내분비기능의 수행에는 유리하나 면역활성물질이나 독성물질등 뇌에 해로운 물질의 왕래를 차단하여 제거하거나, 과다하게 분비된 신경호르몬을 조절하는 일이 중요하다고 볼 때, 큰포식세포가 이같은 역할에 적합한 구성성분이라는 결론을 얻었다. 이 실험에서는 효율성이 높은 관문으로서 혈액-신경뇌하수체관문이 구성성분을 차례로 기재하고 이를 개념화 할 것을 제안한다. 모세혈관과 축삭종말사이에 형성되어 있는 혈액-신경뇌하수체관문이 구성을 차례로 표기하면 다음과 같다 (그림 9,10). 1. 유창모세혈관 내피 2. 모세혈관 내피의 기저판 3. 혈관주위세포와 기저판("이차방어선", 한정된 범위내에서 움직일 수 있다). 4. 큰포식세포, 필요에 따라 판상족(lamellipoda)을 넓게 뻗는다.("삼차방어선", 모세혈관 주위공간 속을 이동해 다니는 "기동타격대") 5. 신경뇌하수체세포의 기저판 6. 신경뇌하수체세포의 세포질돌기(호르몬분비 조절) To study the morphological characteristics of the blood-neurohypophysis barrier system in a severely altered situation, the heads of rats were exposed to heavy X-irradiation. Rats weighing 200-250 g each were anesthetized with sodium thiopenthal, and placed on the table of Mitsubishi linear accelerator ML-4MV. Only heads of rats were placed within the exposure area of 30 cm X 30 cm. Irradiation was processed at the distance of 80 cm, with the speed of 200 rads/min in the radiation depth of 1.2cm. Total doses were 3,000 rads or 6,000 rads according to the animals of the different experimental groups. Rats were sacrificed on 6 hours, 2 days or 6 days following radiations. Tissue blocks of neurohypophyses were fixed in the 1% glutaraldehyde-1% paraformaldehyde solution, and they were refixed in the 1% osmium tetroxide solution. Ultrathin sections were contrasted with uranyl acetate and lead citrate solutions. Electron micrographs exhibited dramatic changes within the perivascular space of neurohypopysis. The perivascular space was greatly enlarged, and it contained many macrophages, floating axonal endings and plentiful flocculent materials. The enlargement was largest in the 2 day-group animals. Many macrophages are activated, and it showed tremendous cytoplasmic lamellipoda. Wide plate of macrophagic cytoplasm usually engulfed the floating axon terminals, and are located between the cappillary and the pituicytes. The situation gave the impression that macrophages prevent, filter and/or retrieve the excessive materials transported between the capillary and the axonal endings. Since the perivascular microenvironment of neurohypophysis is more vulnerable as compared with those brain areas equipped with blood-brain barrier, the functional barrier system by macrophages in the neurohypophysis should have important role. The concept of blood-neurohypophyseal barrier with the following components is proposed. The components of the barrier system from the blood pool to the hormonal pool are 1. Endothelium of fenestrated capillary 2. BAsal lamina of endothelium 3. Pericyte("second line of defense". mobile in a limited area) 4. Macrophage, extending its wide cytoplasmic plate(the lamelipoda) in need("third line of defense". freely movable in the perivascular space, "active surveillance system") 5. Basal lamina of pituicyte 6. Cytoplasmic processes of pituicyte

2-Deoxy-D-Glucose에 의한 대사성 쇼크가 무우스의 면역 반응에 미치는 영향

김양숙,장선일,박래길,조성경,정헌택 최신의학사 1991 最新醫學 Vol.34 No.2

돼지고기육종의 방사선 유래 휘발성 물질 분석

차용준,박지영,김훈,김소정,정연정,소양순,유영재 창원대학교 생활과학연구소 2000 생활과학연구 Vol.4 No.-

Bacillus cereus KCTC 3674 produced at least two kinds of extracellular proteases. Two major bands of the protease activity with molecular weights of approximately 100 and 38 kDa were obtained after gelatin-SDS-PAGE. The production of extracellular proteases occurred during the logarithmic growth phase and was greatest when cultures reached the stationary growth phase. On the other hand, the growth of B. cereus KCTC 3674 and the production of its extracellular proteases were severely inhibited by the addition of protonophore carbonylcyanide m-chlorophenylhydrazone (CCCP). Thus, it was concluded that the growth of B. cereus KCTC 3674 and the production of its extracellular pretenses were strongly affected by a H+ electrochemical potential.

쇠고기육중의 방사선 유래 휘발성물질의 분석

차용준,박지영,김훈,박승영,조우진,송양순,유영재 창원대학교 생활과학연구소 2000 생활과학연구 Vol.4 No.-

This study was conducted to identify irradiation-derived volatile components, having positive-correlation with irradiation doses, which may play roles as maker materials for detecting post-irradiation in pork meat. The volatile components of irradiated(0, 1, 3, 5 and 10 kGy doses) pork(Belly) meat was analyzed by liquid liquid continuous extraction(LLCE)/GC/MSD methods. By the results of linear regression analysis between irradiation doses and volatile component amounts in fresh pork, 3 compounds including γ-octalactone(r=0.82), 1-octen-3-ol(r=0.90) and butanoic acid(r=0.81) had high correlations with irradiation doses.

5일 보존용 채혈백에 따른 보존일자별 농축혈소판의 생화학 및 성상 분석

이동범,유태종,김태전,김승곤,신종관,양대형,고광임,박성훈 임상병리검사과학회 2002 임상병리검사과학회지 Vol.34 No.2

Biochemical changes and icon of platelets on platelet concentrates preserved for a 5day term in several plastic containers were investigated. The authors evaluated the TOTM-PVC second generation container produced by Green Cross Medical Corp. and Boin medica. Blood obtained from 40 persons was manufactured for platelet concentrates within 4 hours and each divided into 20 units of platelet concentrations. Samples were taken at 0, 3, and 5days and the platelet count, pH, pO_(2), pCO_(2), HCO_(3)-, glucose, LDH, lactate, electrolytes, and mineral element were tested The number and MPV, for platelets icon, were well preserved on two different platelet concentrates during storage. A test for biochemical function appeared to be effective data. pH was maintained above 6.8 on two different platelet concentrates during storage. The results suggest that the TOTM-PVC second generation platelets storage container produced by Green Cross Medical Corp. and Boin medica was able to preserve platelets for at least five days without lesion and was it concluded to have biochemical efficacy on patients with transfusions.

Lactoferrin protects against prion protein-induced cell death in neuronal cells by preventing mitochondrial dysfunction.

Park, Yang-Gyu,Jeong, Jae-Kyo,Lee, Ju-Hee,Lee, You-Jin,Seol, Jae-Won,Kim, Shang-Jin,Hur, Tai-Young,Jung, Young-Hun,Kang, Seog-Jin,Park, Sang-Youel D.A. Spandidos 2013 International journal of molecular medicine Vol.31 No.2

<P>Prion disorder-related neurodegenerative diseases are characterized by the accumulation of prion protein (PrP) scrapie isoform (PrPsc) within the central nervous system. PrPsc induces neuronal cell death by increasing intracellular generation of reactive oxygen species (ROS). Lactoferrin (LF) is an 80 kDa protein, which has antioxidant abilities due to the scavenging of ROS. The effects of LF treatment on PrP (106-126)-mediated neurotoxicity and ROS generation were the focus of this study. LF treatment protected against PrP (106-126)-induced neuronal cell death and decreased ROS generation. The reduced ROS generation prevented PrP (106-126)-induced mitochondrial dysfunction. Moreover, PrP (106-126)-induced protein activation including c-Jun N-terminal kinase and caspase-3 were blocked by LF treatment. These results demonstrated that LF protects neuronal cells against PrP (106-126)-mediated neurotoxicity through the scavenging of ROS and provide evidence that LF treatment prevents neuronal cell death caused by PrP (106-126).</P>

Responses of Early-Season Apple Cultivars, ‘Seokwang’ and ‘Sunhong’, to 1-MCP Treatment and Storage Temperatures

Hun J. Kweon,Youn M. Park,In K. kang,Sang J. Yang,Moo Y. Park,Gug S. Choi,Yang Y. Song,Jai K. Cheong 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-