Effects of Crop Straw Application on Micro flora and Enzyme Activity in Continuous Cropping Cucumber Soil in Solar Greenhouse

Yan feng Qi,Min Wei,Xiu feng Wang,Bing hai Du 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Genetic variations in the bitter taste receptor gene TAS2R38 are related to cigarette smoking behavior in Han Chinese smokers

Qi Fei-Yan,Zhu Zhou-Hai,Li Meng,Guan Ying,Peng Qi-Yuan,Lu She-Ming,Liu Zhi-Hua,Wang Ming-Feng,Miao Ming-Ming,Chen Zhang-Yu,Li Xue-Mei,Bai Jie,Yao Jian-Hua 한국유전학회 2022 Genes & Genomics Vol.44 No.11

Background: Smoking behavior is influenced by multiple genes, including the bitter taste gene TAS2R38. It has been reported that the correlation between TAS2R38 and smoking behavior has ethnicity-based differences. However, the TAS2R38 status in Chinese smokers is still unclear. Objective: This study aims to investigate the possible relationship between genetic variations in TAS2R38 (A49P, V262A and I296V) and smoking behaviors in the Han Chinese population. Methods: The haplotype analyses were performed and smoking behavior questionnaire was completed by 1271 individuals. Genetic association analyses for smoking behavior were analyzed using chi-square test. Further, for investigating the molecular mechanism of TAS2R38 variants effect on smoking behavior, we conducted TAS2R38-PAV and TAS2R38-AVI expression plasmids and tested the cellular calcium assay by cigarette smoke compounds stimulus in HEK293. Results: Significant associations of genetic variants within TAS2R38 were identified with smoking behavior. We found a higher PAV/PAV frequency than AVI/AVI in moderate and high nicotine dependence (FTND ≥ 4; X2 = 4.611, 1 df, p = 0.032) and strong cigarette smoke flavor intensity preference (X2 = 4.5383, 1 df, p = 0.033) in participants. Furthermore, in the in vitro cellular calcium assay, total particle matter (TPM), N-formylnornicotine and cotinine, existing in cigarette smoke, activated TAS2R38-PAV but not TAS2R38-AVI-transfected cells. Conclusion: Our data highlights that genetic variations in TAS2R38 are related to smoking behavior, especially nicotine dependence and cigarette smoke flavor intensity preference. Our findings may encourage further consideration of the taste process to identify individuals susceptible to nicotine dependence, particularly Han Chinese smokers.

Functional Analysis of the Inhibitor of Apoptosis Genes in Antheraea pernyi Nucleopolyhedrovirus

Feng Yan,Xiaobei Deng,Junpeng Yan,Jiancheng Wang,Lunguang Yao,Songya lv,Yipeng Qi,Hua Xu 한국미생물학회 2010 The journal of microbiology Vol.48 No.2

The inhibitor of apoptosis proteins (IAP) plays an important role in cell apoptosis. We cloned two novel IAP family members, Ap-iap1 and Ap-iap2, from Antheraea pernyi nucleopolyhedrovirus (ApNPV) genome. Ap-IAP1 contains two baculoviral IAP repeat (BIR) domains followed by a RING domain, but Ap-IAP2 has only one BIR domain and RING. The result of transient expression in Spodoptera frugiperda (Sf21) showed that Ap-iap1 blocked cell apoptosis induced by actinomycin D treatment and also rescued the p35 deficient Autographa californica nucleopolyhedrovirus (AcNPV) to replicate in Sf9 cells, while Ap-iap2 does not have this function. Several Ap-IAP1 truncations were constructed to test the activity of BIRs or RING motif to inhibit cell apoptosis. The results indicated that BIRs or RING of Ap-IAP1 had equally function to inhibit cell apoptosis. Therefore deletion of above both of the above domains could not block apoptosis induced by actinomycin D or rescue the replication of AcMNPV△p35. We also screened two phage-display peptides that might interact with Ap-IAP1.

Field measurements of wind pressure on an open roof during Typhoons HaiKui and SuLi

Feng, Ruoqiang,Liu, Fengcheng,Cai, Qi,Yan, Guirong,Leng, Jiabing Techno-Press 2018 Wind and Structures, An International Journal (WAS Vol.26 No.1

Full-scale measurements of wind action on the open roof structure of the WuXi grand theater, which is composed of eight large-span free-form leaf-shaped space trusses with the largest span of 76.79 m, were conducted during the passage of Typhoons HaiKui and SuLi. The wind pressure field data were continuously and simultaneously monitored using a wind pressure monitoring system installed on the roof structure during the typhoons. A detailed analysis of the field data was performed to investigate the characteristics of the fluctuating wind pressure on the open roof, such as the wind pressure spectrum, spatial correlation coefficients, peak wind pressures and non-Gaussian wind pressure characteristics, under typhoon conditions. Three classical methods were used to calculate the peak factors of the wind pressure on the open roof, and the suggested design method and peak factors were given. The non-Gaussianity of the wind pressure was discussed in terms of the third and fourth statistical moments of the measured wind pressure, and the corresponding indication of the non-Gaussianity on the open roof was proposed. The result shows that there were large pulses in the time-histories of the measured wind pressure on Roof A2 in the field. The spatial correlation of the wind pressures on roof A2 between the upper surface and lower surface is very weak. When the skewness is larger than 0.3 and the kurtosis is larger than 3.7, the wind pressure time series on roof A2 can be taken as a non-Gaussian distribution, and the other series can be taken as a Gaussian distribution.

FORMATION OF II–VI SEMICONDUCTOR NANOCRYSTALS WITH TUNABLE VISIBLE EMISSION IN AQUEOUS SOLUTION PROMOTED BY HYDRAZINE

QI-HUI SHEN,YAN LIU,XI YU,XIAO-YANG LIU,MING-QIANG ZOU,JIN-FENG LI,JIAN-GUANG ZHOU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2012 NANO Vol.7 No.6

II?VI Semiconductor nanocrystals (NCs) with tunable visible emission, such as CdS, CdSe and CdTe, were synthesized in aqueous solution using thiols as capping molecules. Hydrazine was found to promote the growth of NCs through a special mechanism. In only a few hours, the synthesis process was completed at room temperature. Under moderate conditions, the capping molecules not only changed the growth rate of NCs simply by varying the concentration, but also altered the spectral properties of NCs. The capping molecules with amino groups were propitious to the growth of CdS NCs, whereas the kinetic growth of CdS NCs was more a®ected by the surface passivation e±ciency of NCs than by steric hindrance in the system. The fastest growth of the CdS NCs was achieved when glutathione was used as a capping molecule, while the emission of CdS and CdSe NCs were shown to remain steady and tunable using the same capping molecule. The growth rate of 3-mercaptopropionic acid-capped CdS and CdSe NCs slowed down signi¯-cantly, while CdTe NCs were obtained with excellent emission properties when capped with the same molecule. Furthermore, our approach will also be useful for the study of the kinetic growth of NCs in aqueous solution.

Knockdown of endogenous SKIP gene enhance insulin-induced glycogen synthesis signaling differentiating C2C12 myoblasts

( Qi Xiong ),( Chang Yan Deng ),( Jin Chai ),( Si Wen Jiang ),( Yuan Zhu Xiong ),( Feng E Li ),( Rong Zheng ) 생화학분자생물학회 2009 BMB Reports Vol.42 No.2

Relationship Between Dry Ports and Regional Economy: Evidence from Yangtze River Economic Belt*

Yan Feng LIU,Chong Bae LEE,Guan Qiu QI,Kum Fai YUEN,Miao SU 한국유통과학회 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.5

With the evolution of containerization and globalization of supply chains, aspects of port functions have made the transition from the sea to the inland region that forms the dry port. To explore the relationship between dry ports and regional economic development, this study uses a gravity model and forecast model to analyze 1,040 observations in 104 cities (22 dry port cities) along the Yangtze River Economic Belt (YREB) from 2008 to 2017. The model includes economic variables, logistics variables, foreign relations variables, and human capital variables. It was found that the dry port is positively correlated with trade volume. Compared with a city without a dry port, the trade volume of a city with a dry port will increase 0.099 times. It can be concluded that a dry port is crucial for the economic development of the YREB. It was also found that per capita GDP as an economic variable, road area and rail number as logistics variables, and foreign relation variables are positively correlated with trade volume, while the human capital variable has no significant effect on trade volume. In addition, governmental policy implications are addressed from the aspects of dry port and industry cluster caused by foreign investment.

Optimal Operation Schedule Strategy of High-power Pulsed Loads in Shipboard Power System

Zhang Yan,Ji Feng,Gao Xueping,Ma Fan,Hu Qi 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.4

With the vigorous development of the scientifi c and technological revolution, new military and engineering loads with high power and pulse characteristics are becoming reality for the shipboard power system (SPS) application. These loads are usually called high-power pulsed loads (HPPLs) because of their high peak power, low average power and short cycle time. The HPPLs can cause voltage drop and even system instability to the SPS, thus bringing severe challenges to their power supplying system. To address these issues, an optimal operation schedule of HPPLs in SPS is studied in this paper. Firstly, the models of HPPLs for SPS power optimization are proposed based on the real back-end load connection way of them. Then the mixed integer programming problem aiming at maximizing the operation effi ciency of the HPPLs within the SPS support capability is constructed. Finally, some typical cases are studied to verify the fl exibility and eff ectiveness of the proposed strategy. The results indicate that the proposed strategy can eff ectively maximize the operational effi ciency of HPPLs in the SPS, and the superiority becomes more obvious as the number of HPPLs increases.

Comparative genomic analysis of Mycoplasma anatis strains

Zhou Qi,Mai Kaijie,Yang Dehong,Liu Junfa,Yan Zhuanqiang,Luo Cuifen,Tan Yangtong,Cao Sheng,Zhou Qingfeng,Chen Li,Chen Feng 한국유전학회 2021 Genes & Genomics Vol.43 No.11

Background The Gram-negative intracellular bacterium Mycoplasma anatis is a pathogen of respiratory infectious diseases in ducks and has caused signifcant economic losses in the poultry industry. Objective This study, as the frst report of the structure and function of the pan-genome of Mycoplasma anatis, may provide a valuable genetic basis for many aspects of future research on the pathogens of waterfowl. Methods We sequenced the whole genomes of 15 Mycoplasma anatis isolated from ducks in China. Draft genome sequencing was carried out and whole-genome sequencing was performed by the sequencers of the PacBio Sequel and an IonTorrent Personal Genome Machine (PGM). Then the common genic elements of protein-coding genes, tRNAs, and rRNAs of Mycoplasma anatis genomes were predicted by using the pipeline Prokka v1.13.7. To investigate homologous protein clusters across Mycoplasma anatis genomes, we adopted Roary v3.13.0 to cluster orthologous genes (OGs) based on the following criteria. Results We obtained one complete genome and 14 genome sketches. Microbial mobile genetic element analysis revealed the distribution of insertion sequences (IS30, IS3, and IS1634), prophage regions, and CRISPR arrays in the genome of Mycoplasma anatis. Comparative genomic analysis decoded the genetic components and functional classifcation of the pan-genome of Mycoplasma anatis that comprised 646 core genes, 231 dispensable genes and among them 110 was strainspecifc. Virulence-related gene profles of Mycoplasma anatis were systematically identifed, and the products of these genes included bacterial ABC transporter systems, iron transport proteins, toxins, and secretion systems. Conclusion A complete virulence-related gene profle of Mycoplasma anatis has been identifed, most of the genes are highly conserved in all strains. Sequencing results are relevant to the molecular mechanisms of drug resistance, adaptive evolution of pathogens, population structure, and vaccine development.

Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China

Wei-Feng Qian,Wen-Chao Yan,Tian-Qi Wang,Kai Zhai,Li-Fang Han,Chao-Chao Lv 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1

The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3’+5’) SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29- 2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.