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Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China
Wei-Feng Qian,Wen-Chao Yan,Tian-Qi Wang,Kai Zhai,Li-Fang Han,Chao-Chao Lv 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1
The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3’+5’) SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29- 2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.
( Wen Qian Wei ),( Fang Qi Liu ),( Lei Liu ),( Zuo Feng Li ),( Xiao Yan Zhang ),( Fan Jiang ),( Qu Shi ),( Xiao Yan Zhou ),( Wei Qi Sheng ),( San Jun Cai ),( Xuan Li ),( Ye Xu ),( Peng Nan ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.5
Hereditary non-polyposis Colorectal Cancer (HNPCC) is an autosomal dominant inheritance syndrome. HNPCC is the most common hereditary variant of colorectal cancer (CRC), which accounts for 2-5% CRCs, mainly due to hMLH1 and hMSH2 mutations that impair DNA repair functions. Our study aimed to identify the patterns of hMSH2 and hMLH1 mutations in Chinese HNPCC patients. Ninety-eight unrelated families from China meeting Amsterdam or Bethesda criteria were included in our study. Germline mutations in MLH1 and MSH2 genes, located in the exons and the splice-site junctions, were screened in the 98 probands by direct sequencing. Eleven mutations were found in ten patients (11%), with six in MLH1 (54.5%) and five in MSH2 (45.5%) genes. One patient had mutations in both MLH1 and MSH2 genes. Three novel mutations in MLH1 gene (c.157_160delGAGG, c.2157dupT and c.-64G>T) were found for the first time, and one suspected hotspot in MSH2 (c.1168C>T) was revealed. [BMB reports 2011; 44(5): 317-322]
Wei, Guangfei,Yang, Feng,Wei, Fugang,Zhang, Lianjuan,Gao, Ying,Qian, Jun,Chen, Zhongjian,Jia, Zhengwei,Wang, Yong,Su, He,Dong, Linlin,Xu, Jiang,Chen, Shilin The Korean Society of Ginseng 2020 Journal of Ginseng Research Vol.44 No.6
Background: Panax quinquefolius and Panax notoginseng are widely used and well known for their pharmacological effects. As main pharmacological components, saponins have different distribution patterns in the root tissues of Panax plants. Methods: In this study, the representative ginsenosides were detected and quantified by desorption electrospray ionization mass spectrometry and high-performance liquid chromatography analysis to demonstrate saponin distribution in the root tissues of P. quinquefolius and P. notoginseng, and saponin metabolite profiles were analyzed by metabolomes to obtain the biomarkers of different root tissues. Finally, the transcriptome analysis was performed to demonstrate the molecular mechanisms of saponin distribution by gene profiles. Results: There was saponin distribution in the root tissues differed between P. quinquefolius and P. notoginseng. Eight-eight and 24 potential biomarkers were detected by metabolome analysis, and a total of 340 and 122 transcripts involved in saponin synthesis that were positively correlated with the saponin contents (R > 0.6, P < 0.05) in the root tissues of P. quinquefolius and P. notoginseng, respectively. Among them, GDPS1, CYP51, CYP64, and UGT11 were significantly correlated with the contents of Rg1, Re, Rc, Rb2, and Rd in P. quinquefolius. UGT255 was markedly related to the content of R1; CYP74, CYP89, CYP100, CYP103, CYP109, and UGT190 were markedly correlated with the Rd content in P. notoginseng.
MicroRNA-206 Reduces Osteosarcoma Cell Malignancy In Vitro by Targeting the PAX3-MET Axis
Qian-Rong Deng,Fang-Biao Zhan,Xian-Wei Zhang,Shi-Long Feng,Jun Cheng,You Zhang,Bo Li,Li-Zhong Xie 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.2
Purpose: This study was undertaken to explore how miR-206 represses osteosarcoma (OS) development. Materials and Methods: Expression levels of miR-206, PAX3, and MET mRNA were explored in paired OS and adjacent tissuespecimens. A patient-derived OS cell line was established. miR-206 overexpression and knockdown were achieved by lentiviraltransduction. PAX3 and MET overexpression were achieved by plasmid transfection. Treatment with hepatocyte growth factor(HGF) was utilized to activate c-Met receptor. Associations between miR-206 and PAX3 or MET mRNA in OS cells were verifiedby AGO2-RNA immunoprecipitation assay and miRNA pulldown assay. OS cell malignancy was evaluated in vitro by cell proliferation,metastasis, and apoptosis assays. PAX3 and MET gene expression in OS cells was assayed by RT-qPCR and Western blot. Activation of PI3K-AKT and MAPK-ERK in OS cells were assayed by evaluating Akt1 Ser473 phosphorylation and total threoninephosphorylation of Erk1/2, respectively. Results: Expression levels of miR-206 were significantly decreased in OS tissue specimens, compared to adjacent counterparts,and were inversely correlated with expression of PAX3 and MET mRNA. miR-206 directly interacted with PAX3 and MET mRNAin OS cells. miR-206 overexpression significantly reduced PAX3 and MET gene expression in OS cells in vitro, resulting in significantdecreases in Akt1 and Erk1/2 activation, cell proliferation, and metastasis, as well as increases in cell apoptosis, while miR-206 knockdown showed the opposite effects. The effects of miR-206 overexpression on OS cells were reversed by PAX3 or METoverexpression, but only partially attenuated by HGF treatment. Conclusion: miR-206 reduces OS cell malignancy in vitro by targeting PAX3 and MET gene expression.
Soil and ribbed concrete slab interface modeling using large shear box and 3D FEM
Qian, Jian-Gu,Gao, Qian,Xue, Jian-feng,Chen, Hong-Wei,Huang, Mao-Song Techno-Press 2017 Geomechanics & engineering Vol.12 No.2
Cast in situ and grouted concrete helical piles with 150-200 mm diameter half cylindrical ribs have become an economical and effective choice in Shanghai, China for uplift piles in deep soft soils. Though this type of pile has been successful used in practice, the reinforcing mechanism and the contribution of the ribs to the total resistance is not clear, and there is no clear guideline for the design of such piles. To study the inclusion of ribs to the contribution of shear resistance, the shear behaviour between silty sand and concrete slabs with parallel ribs at different spacing and angles were tested in a large direct shear box ($600mm{\times}400mm{\times}200mm$). The front panels of the shear box are detachable to observe the soil deformation after the test. The tests were modelled with three-dimensional finite element method in ABAQUS. It was found that, passive zones can be developed ahead of the ribs to form undulated failure surfaces. The shear resistance and failure mode are affected by the ratio of rib spacing to rib diameter. Based on the shape and continuity of the failure zones at the interface, the failure modes at the interface can be classified as "punching", "local" or "general" shear failure respectively. With the inclusion of the ribs, the pull out resistance can increase up to 17%. The optimum rib spacing to rib diameter ratio was found to be around 7 based on the observed experimental results and the numerical modelling.
Ya-Feng Song,Xiong-Xiong Kong,Wei-Bin Tang,Zhong-Qiang Suo,Huan Zhang,Chen-Yang Li,Qian Jia,Cai-Xia Xue,Yan-Wu Lu,Chao-Pu Yang 한국물리학회 2019 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.74 No.11
We investigate the polarization effect in intersubband transitions in polar and nonpolar GaN-based multiple-quantum well (MQW) structures for terahertz (THz) emissions by using systematic comparisons and design a nonpolar GaN/Al$_{0.2}$Ga$_{0.8}$N two-well-based MQW structure with an emitting photon of 7.27 THz (30.07 meV). Its lower energy separation (92.7 meV) matches the resonant phonon depopulation condition for better population inversion. It shows a lower threshold current density $J\rm_{th}$ at all temperatures (1.548 kA/cm$^2$ at 90 K) and a higher output power of up to 86.1 mW at 5.8 K and 33.6 mW at 100 K. Our results for the polar GaN MQW are very close to the experimental data in the literature. We find that the $J\rm_{th}$ of the nonpolar GaN MQW increases more slowly than that of the polar GaN MQW as temperature increases, indicating the nonpolar GaN MQW may be a worth-trying direction for improving the operation temperature. These results can provide meaningful references for the design and fabrication of nonpolar GaN-based THz MQW or quantum cascade structures.
Loss of MicroRNA-137 Impairs the Homeostasis of Potassium in Neurons via KCC2
Ting-Wei Mi,Xiao-Wen Sun,Zhi-Meng Wang,Ying-Ying Wang,Xuan-Cheng He,Cong Liu,Shuang-Feng Zhang,Hong-Zhen Du,Chang-Mei Liu,Zhao-Qian Teng 한국뇌신경과학회 2020 Experimental Neurobiology Vol.29 No.2
Neuropsychiatric disorders are the leading cause of mental and intellectual disabilities worldwide. Current therapies against neuropsychiatric disorders are very limited, and very little is known about the onset and development of these diseases, and their most effective treatments. MIR137 has been previously identified as a risk gene for the etiology of schizophrenia, bipolar disorder, and autism spectrum disorder. Here we generated a forebrain-specific MIR137 knockout mouse model, and provided evidence that loss of miR-137 resulted in impaired homeostasis of potassium in mouse hippocampal neurons. KCC2, a potassium-chloride co-transporter, was a direct downstream target of miR-137. The KCC2 specific antagonist VU0240551 could balance the current of potassium in miR-137 knockout neurons, and knockdown of KCC2 could ameliorate anxiety-like behavior in MIR137 cKO mice. These data suggest that KCC2 antagonists or knockdown might be beneficial to neuropsychiatric disorders due to the deficiency of miR-137.
OK-432 Suppresses Proliferation and Metastasis by Tumor Associated Macrophages in Bladder Cancer
Tian, Yuan-Feng,Tang, Kun,Guan, Wei,Yang, Tao,Xu, Hua,Zhuang, Qian-Yuan,Ye, Zhang-Qun Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11
OK-432, a Streptococcus-derived anticancer immunotherapeutic agent, has been applied in clinic for many years and achieved great progress in various cancers. In the present study, we investigated its anticancer effect on bladder cancer through tumor associated macrophages (TAMs). MTS assay validated OK-432 could inhibit proliferation in both T24 and EJ bladder cell lines. OK-432 also induced apoptosis of bladder cancer cells in vitro. Consequently, we demonstrated that OK-432 could suppress the bladder cancer cells migration and invasion by altering the EMT-related factors. Furthermore, using SD rat model, we revealed that OK-432 inhibited tumor growth, suppressed PCNA expression and inhibited metastasis in vivo. Taken together, these findings strongly suggest that OK-432 inhibits cell proliferation and metastasis through inducing macrophages to secret cytokines in bladder cancer.
Zhonglin Chen,Qian Zhou,Feng Leng,Bing Dai,Xueyang Zhao,Weichen Zhang,Neng Liu,Wei Guan,Kui Liu 대한환경공학회 2017 Environmental Engineering Research Vol.22 No.3
The joint effect of different concentrations (0.05-0.375 mmol/L) of Cd<SUP>2+</SUP> and various concentrations (50-400 mg/L) of [C3mim][OAc] on the growth and physiology of wheat seedlings were investigated. The toxicity of Cd<SUP>2+</SUP> could be significantly reduced by lower concentrations (50-200 mg/L) of [C3mim][OAc]. With higher concentration (≥ 300 mg/L) of [C3mim][OAc], the reduction became less, and compared to Cd<SUP>2+</SUP> stress only, the toxicity of 400 mg/L [C3mim][OAc] increased. As for different Cd<SUP>2+</SUP> concentrations, the optimal reduction depends on the balance between the exact concentration of Cd<SUP>2+</SUP> and [C3mim][OAc]: With low Cd<SUP>2+</SUP> concentration (0.05-0.250 mmol/L) requiring 50 mg/L [C3mim][OAc], it could reach a dynamic equilibrium, while high Cd<SUP>2+</SUP> concentration (0.375 mmol/L) requiring 100 mg/L [C3mim][OAc]. The growth and physiological indexes of wheat seedlings show a decrease in toxicity compared to the Cd alone treatment, when the dynamic equilibrium is reached. The concentration of Cd<SUP>2+</SUP> in leaf tissues showed that low concentration (50-200 mg/L) of [C3mim][OAc] can reduce the toxicity of Cd<SUP>2+</SUP> (0.05-0.375 mmol/L) by decreasing the level of Cd<SUP>2+</SUP> concentration in the tissues, whereas the higher concentration (≥ 300 mg/L) of [C3mim][OAc] can increase the concentration of Cd<SUP>2+</SUP>.