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( Jian Cao ),( Yuan Qing He ),( Guo Hui Li ),( Ke Ping Chen ),( Jie Kong ),( Feng Hua Wang ),( Jing Shi ),( Qin Yao ) 한국잠사학회 2011 International Journal of Industrial Entomology Vol.22 No.2
Protein kinase C (PKC) is involved in many cellular signaling pathways, it participates in many physiological processes, such as cell cycle, growth, proliferation, differentiation and apoptosis. To investigate the effect of PKC on the silkworm midgut tissue infection of Bombyx mori parvo-like virus (BmPLV), a B. mori atypical protein kinase C (BmaPKC) gene was cloned from larval midgut tissue, expressed in E. coli and purified. Additionally, the BmPLV susceptible silkworm strain and resistant silkworm strain were used to test the effect of the B. mori infection on BmPLV. The result showed that BmaPKC encodes a predicted 586 amino acid protein, which contains a C-terminal kinase domain and an N-terminal regulatory domain. The maximum expression amount of the soluble (His)6-tagged fusion protein was detected after 0.8 mmol/L IPTG was added and cultured at 21˚C. The (His) 6-tagged fusion protein revealed about 73 kDa molecular weight which confirmed by western blot and mass spectrography. Furthermore BmaPKC protein were detected at 0-72 h post-infection in BmPLVinfected larval midgut tissue, western blot showed that as time went on, the expression of BmaPKC increased gradually in susceptible strain, the expression quantity on 72 h is 5 times of 0 h. However, in resistant strain, the expression quantity is slightly lower than susceptible strain. But no significant change in resistant strain was observed as time went on. The available data suggest that BmaPKC may involve in the regulation of BmPLV proliferation.
Yao, Zeng-Yu,Qi, Jian-Hua Institute of Forest Science 2016 Journal of Forest Science Vol.32 No.3
Xanthoceras sorbifolia seed coat (XSSC) is a processing residue of the bioenergy crop. This work aimed to evaluate the applicability of using the steam explosion to modify the residue for dye biosorption from aqueous solutions by using methylene blue as a model cationic dye. Equilibrium, kinetic and thermodynamic parameters for the biosorption of methylene blue on the steam-exploded XSSC (SE-XSSC) were evaluated. The kinetic data followed the pseudo-second-order model, and the rate-limiting step was the chemical adsorption. Intraparticle diffusion was one of the rate-controlling factors. The equilibrium data agreed well with the Langmuir isotherm, and the biosorption was favorable. The steam-explosion pretreatment strongly affected the biosorption in some respects. It reduced the adsorption rate constant and the initial sorption rate of the pseudo-second-order model. It enhanced the adsorption capacity of methylene blue at higher temperatures while reduced the capacity at lower ones. It changed the biosorption from an exothermic process driven by both the enthalpy and the entropy to an endothermic one driven by entropy only. It increased the surface area and decreased the pH point of zero charge of the biomass. Compared with the native XSSC, SE-XSSC is preferable to MB biosorption from warmer dye effluents.
Yao-zhong Ding,Jian-hua Zhou,Li-na Ma,Yan-ni Qi,Gang Wei,Jie Zhang,Yong-guang Zhang 대한수의학회 2014 JOURNAL OF VETERINARY SCIENCE Vol.15 No.3
A reverse transcription loop-mediated isothermalamplification (RT-LAMP) assay was developed to rapidlydetect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples,sensitivity of the FMDV C RT-LAMP was found to be 10times higher than that of conventional reverse transcription-PCR (RT-PCR). No cross-reactivity with A, Asia 1, or OFMDV or swine vesicular disease virus (SVDV) indicatedthat FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.
Effects of MicroRNA-106 on Proliferation of Gastric Cancer Cell through Regulating p21 and E2F5
Yao, Yong-Liang,Wu, Xiao-Yang,Wu, Jian-Hong,Gu, Tao,Chen, Ling,Gu, Jin-Hua,Liu, Yun,Zhang, Qing-Hui Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5
Objective: To investigate the effects of miR-106b on malignant characteristics of gastric cancer cells, and explore possible mechanisms. Methods: Expression of miR-106b, p21 and E2F was determined by real-time PCR. Transfection with miR-106b mimics was conducted, and gastric cancer cells with miR-106b overexpression were obtained. Cells transfected with mimic mutants and those without transfection served as negative and blank controls, respectively. Flow cytometry and transwell assays were adopted to detect the effects of miR-106b overexpression on cell cycle, migration and invasion of gastric cancer cells. Results:. The expression of miR- 106b in gastric cancer cells was significantly higher than that in normal gastric mucosa cells. Furthermore, the expression level of miR-106b rose according to the degree of malignacy among the three GC cell strains (MKN- 45 > SGC-7901 > MKN-28). Overexpression of miR-106b shortened the G0/G1 phase and accelerated cell cycle progression, while reducing p21 and E2F5, without any significant effects on the capacity for migration and invasion of gastric cancer cells. Conclusions: miR-106b may promote cell cycling of gastric cancer cells through regulation of p21 and E2F5 target gene expression.
Low Specific On-resistance SOI LDMOS Device with P<sup>+</sup>P-top Layer in the Drift Region
Yao, Jia-Fei,Guo, Yu-Feng,Xu, Guang-Ming,Hua, Ting-Ting,Lin, Hong,Xiao, Jian The Institute of Electronics and Information Engin 2014 Journal of semiconductor technology and science Vol.14 No.5
In this paper, a novel low specific on-resistance SOI LDMOS Device with P+P-top layer in the drift region is proposed and investigated using a two dimensional device simulator, MEDICI. The structure is characterized by a heavily-doped $P^+$ region which is connected to the P-top layer in the drift region. The $P^+$ region can modulates the surface electric field profile, increases the drift doping concentration and reduces the sensitivity of the breakdown voltage on the geometry parameters. Compared to the conventional D-RESURF device, a 25.8% decrease in specific on-resistance and a 48.2% increase in figure of merit can be obtained in the novel device. Furthermore, the novel $P^+P$-top device also present cost efficiency due to the fact that the $P^+$ region can be fabricated together with the P-type body contact region without any additional mask.
Rapid preparation and characterization of chitosan nanoparticles for oligonucleotide
Mu-hua Cheng,Yao-xiong Huang,Han-jian Zhou,Zhi Liu,Jian-fang Li 한국물리학회 2010 Current Applied Physics Vol.10 No.3
Chitosan is regarded as one of the potential candidates as a gene carrier. However, the poor solubility of chitosan is the major limiting factor in its utilization as a gene carrier. The purpose of this study was to simplify the method of preparing the nanoparticles of chitosan linked with antisense oligonucleotide (asON). The main step was preparing the derivatives of chitosan phosphate (CSP) in order to easily dissolve in aqueous solution. The nanoparticles were formed using a simple mixed method for CSP and asON, and the nanoparticle’s forming condition was optimized so that the nanoparticle’s characterization could be examined. Results showed that it was simple to make the nanoparticles under the optimal condition of 2:1 M proportion of CSP and asON. The size of the nanoparticles was 102.6 ± 12.0 nm, its zeta potential was 1.45 ± 1.75, and the encapsulated ratio of the chitosan crosslinked the asON was 87.6 ± 3.5%. The infrared spectra and electron microscope displayed that chitosan may combine with the asON to form equirotal nanoparticles. In conclusion, it was simple and feasible to form chitosan nanoparticles for asON using the CSP, and the CSP can efficiently encapsulate asON.
Low Specific On-resistance SOI LDMOS Device with P<SUP>+</SUP>P-top Layer in the Drift Region
Jia-Fei Yao,Yu-Feng Guo,Guang-Ming Xu,Ting-Ting Hua,Hong Lin,Jian Xiao 대한전자공학회 2014 Journal of semiconductor technology and science Vol.14 No.5
In this paper, a novel low specific onresistance SOI LDMOS Device with P<SUP>+</SUP>P-top layer in the drift region is proposed and investigated using a two dimensional device simulator, MEDICI. The structure is characterized by a heavily-doped P<SUP>+</SUP> region which is connected to the P-top layer in the drift region. The P<SUP>+</SUP> region can modulates the surface electric field profile, increases the drift doping concentration and reduces the sensitivity of the breakdown voltage on the geometry parameters. Compared to the conventional D-RESURF device, a 25.8% decrease in specific on-resistance and a 48.2% increase in figure of merit can be obtained in the novel device. Furthermore, the novel P<SUP>+</SUP>P-top device also present cost efficiency due to the fact that the P<SUP>+</SUP> region can be fabricated together with the P-type body contact region without any additional mask.
Zeng-Yu Yao,Jian-Hua Qi 강원대학교 산림과학연구소 2016 Journal of Forest Science Vol.32 No.3
Xanthoceras sorbifolia seed coat (XSSC) is a processing residue of the bioenergy crop. This work aimed to evaluate the applicability of using the steam explosion to modify the residue for dye biosorption from aqueous solutions by using methylene blue as a model cationic dye. Equilibrium, kinetic and thermodynamic parameters for the biosorption of methylene blue on the steam-exploded XSSC (SE-XSSC) were evaluated. The kinetic data followed the pseudo-second- order model, and the rate-limiting step was the chemical adsorption. Intraparticle diffusion was one of the rate-controlling factors. The equilibrium data agreed well with the Langmuir isotherm, and the biosorption was favorable. The steam-explosion pretreatment strongly affected the biosorption in some respects. It reduced the adsorption rate constant and the initial sorption rate of the pseudo-second-order model. It enhanced the adsorption capacity of methylene blue at higher temperatures while reduced the capacity at lower ones. It changed the biosorption from an exothermic process driven by both the enthalpy and the entropy to an endothermic one driven by entropy only. It increased the surface area and decreased the pH point of zero charge of the biomass. Compared with the native XSSC, SE-XSSC is preferable to MB biosorption from warmer dye effluents.