Methotrexate 치료를 받고 있는 활동성 류마티스 관절염 환자에서 etanercept의 유효성과 안전성에 관한 연구

최병렬,강태영,정청일,이혜순,엄완식,김태환,전재범,유대현,배상철 대한내과학회 2004 대한내과학회지 Vol.66 No.5

목적 : 류마티스 관절염을 앓고 있는 한국인 중에 기존 DMARDs로 치료 실패하였고, 고정용량의 MTX를 복용하고 있는 환자들에 있어서 etanercept의 유효성과 안전성을 평가하고자 하였다. 방법 : 기존 DMARDs로 치료 실패한 활동성 류마티스 관절염 환자 76명을 대상으로 하여 단일군, 공개시험을 하였다. 대상 환자들은 고정용량의 MTX를 복용하면서 etanercept 25 mg을 1주일에 두 번 피하 주사하였으며 12주간 투여하였다. 유효성은 ACR 20, ACR 50,조조강직 시간으로 평가하였고, 약제의 안전성은 이상반응 등으로 평가하였다. 결과 : 대상 환자는 총 76명으로 평균 연령은 45.2세, 남자 5명, 여자 71명이었다. 84.4%인 54명이 12주째에 ACR 20을 만족하였고, 53.1%인 34명이 12주째에 ACR 50을 만족하였다. 조조 강직 시간은 치료 전 203.3분에서 치료 12주째 42.6분을 평균 74.5% 호전되었다. 가장 흔한 이상반응은 주사부위 반응이었다. 이외에도 상기도 감염, 오심, 안면부종 등이 발생하였으나 심각한 부작용은 없었다. 결론 : etanercept는 효과적이고, 안전한 류마티스 관절염 치료 방법이며 특히 MTX치료에도 불구하고 활동성인 류마티스 관절염에 기대되는 치료라고 할 수 있다. Background : This study was performed to investigate the efficacy and safety of etanercept in active rheumatoid arthritis patients with stable dose of methotrexate in Korean. Methods : In a 12 week, single arm, open trial, we assigned 76 patients with active rheumatoid arthritis who had an inadequate response to disease-modifying antirheumatic drugs. Patients received twice-weekly subcutaneous injections of etanercept 25 ㎎ while containing to receive methotrexate at a stable dose of 7.5~25 ㎎ per week. The clinical response was defined as the percent improvement in disease activity according to the criteria of the American Collage of Rheumatology (ACR) at 12 weeks. Results : Etanrecept led to significant improvements in disease activity and was safe and well tolerated. At 12 week, 84.4% of the patients receiving 25 ㎎ of etanercept achieved a 20% ACR response, and 53.1% of those receiving etanercept achieved a 50% ACR response. The most common adverse event was injection-site reaction. Other advanse events were upper respiratory infection, nausea, and facial edema, but there were no serious adverse events associated with etanercept. Conclusion : In active rheumatoid arthritis patients, etanercept was safe, well tolerated, and provided rapid clinical improvements.

제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

A study on the characteristics of Maslach Burnout Inventory-General Survey (MBI-GS) of workers in one electronics company

Young Gon Choi,Byung Jin Choi,Tae Hwi Park,Jun Young Uhm,Dong Bae Lee,Seong Sil Chang,Soo Young Kim 대한직업환경의학회 2019 대한직업환경의학회지 Vol.31 No.-

Background: This study investigated characteristics according to demographic, occupational factors of Maslach Burnout Inventory-General Survey (MBI-GS) and related scales to MBI-GS. Methods: The subjects of the study were 3,331 workers in 3 different workplaces of one electronics company. They filled in demographic factors surveys, occupational factors surveys, MBI-GS, Korean Occupational Stress Scale-Short Form (KOSS-SF), Patient Health Questionnaire-9 (PHQ-9), and World Health Organization Quality Of Life-Abbreviated version (WHOQOL-BREF). The correlations between sub-scales of MBI-GS and KOSS-SF, PHQ-9, WHOQOL-BREF were analyzed respectively. And KOSS-SF, PHQ-9, and WHOQOL-BREF were categorized; mean scores of sub-scales of MBI-GS were compared; and the quartiles of sub-scales of MBI-GS were presented. Results: A comparison of mean scores of MBI-GS according to demographic and occupational factors showed a significant difference according to age, problem drinking behavior, working time, and working duration in exhaustion regardless of sex. In professional efficacy, a significant difference was observed in age, marital status, working type, and working duration. And as a result of correlation analysis, the correlation coefficient between exhaustion and PHQ-9 was the highest regardless of sex. In addition, regardless of sex, exhaustion and cynicism scores tended to increase and professional efficacy score tended to decrease as the work stress level rose. Same tendency is shown in case of the more severe the symptom of depression and the lower quality of life. When the quartile for sub-scales" score of MBI-GS were investigated, the burnout was more pronounced in female than in male. Conclusions: Many demographic and occupational factors affect burnout were identified in one electronics company, and we investigated which sub-scales of MBI-GS were affected. Through this study, burnout characteristics were identified in a few population group of Korea, and the results are expected to be useful for burnout risk group identification, counseling, etc.

영상 분석을 통한 우리 춤동작의 특성 이해

엄태영(Tae-Young Uhm),박한훈(Hanhoon Park),박종일(Jong-Il Park),김운미(Un-Mi Kim) 한국HCI학회 2006 한국HCI학회 학술대회 Vol.2006 No.2

The association between shift work and chronic kidney disease in manual labor workers using data from the Korea National Health and Nutrition Examination Survey (KNHANES 2011–2014)

Jun Young Uhm,Hyoung-Ryoul Kim,Gu Hyeok Kang,Young Gon Choi,Tae Hwi Park,Soo Young Kim,Seong Sil Chang,Won Oh Choo 대한직업환경의학회 2019 대한직업환경의학회지 Vol.30 No.-

Objective: Kidneys are organs having a biological clock, and it is well known that the disruption of the circadian rhythm increases the risk of chronic kidney disease (CKD), including the decline of renal and proteinuria. Because shift work causes circadian disruption, it can directly or indirectly affect the incidence of chronic kidney disease. Therefore, the purpose of this study was to investigate the association between shift work and chronic kidney disease using a Korean representative survey dataset. Methods: This study was comprised of 3504 manual labor workers over 20 years of age from data from the fifth and sixth Korea National Health and Nutrition Examination Survey (2011–2014). The work schedules were classified into two types: day work and shift work. The estimated glomerular filtration rate, which is the ideal marker of renal function, was estimated according to the Chronic Kidney Disease Epidemiology Collaboration creatinine equation, and chronic kidney disease was defined as urinary albumin to a creatinine ratio equal to or high than 30 mg/g and/or estimated glomerular filtration rate lower than 60 mL/min/1.73m2. The cross-tabulation analysis and multivariate logistic regression analysis were performed to confirm the association between shift work and chronic kidney disease stratified by gender. Results: The risk of CKD showed a significant increase (odds ratio = 2.04, 95% confidence interval = 1.22, 3.41) in the female worker group. The same results were obtained after all confounding variables were adjusted (odds ratio = 2. 34, 95% confidence interval = 1.35, 4.07). However, the results of the male worker group were not significant. Conclusions: In this study using nationally representative surveys, we found that the risk of CKD was higher female workers and shift work. Future prospective cohort studies will be needed to clarify the causal relationship between shift work and CKD.

차량 운전석 모듈의 진동해석을 통한 구조 소음 발생원 예측에 관한 연구

엄호영(Ho-Young Uhm),김배영(Bae-Young Kim),신희종(Hee-Jong Shin),박경태(Gyeong-Tae Park),허하영(Ha-Young Heo),서명원(Myung-won Suh) 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.11

Among the various elements affecting a customer's evaluation of automobile quality, buzz, squeak, and rattle (BSR) are considered to be major factors. We propose an analytical method that can be used to reduce BSR in a cockpit module in a vehicle, especially during the early design stage of vehicle development Traditionally BSR in subsystems and components of a vehicle have been identifed and rectifed through extensive hardware testing. With the auto companies and supplier being challenged to cut structural costs, eliminate costly hardware building, and bring products to market fester by reducing development cycles, increasing math analysis of subsystems and components for such perceived quality issues are desirable.

활락탕을 투여한 편타성 손상 증후군 환자 치험 2례

엄봉군,조태영,정영훈,이차로,이재영,안영태,Uhm, Bong-Kun,Cho, Tae-Young,Jung, Young-Hun,Lee, Cha-Ro,Lee, Jae-Young,Ahn, Young-Tae 척추신경추나의학회 2010 척추신경추나의학회지 Vol.5 No.1

활락탕을 처방하여 편타성 손상으로 경부 및 상지에 극심한 통증이 호전된 2례를 통해 편타성 손상의 초기 단계인 경우(1-10 일(日)) 활혈거어지제(活血祛瘀之劑)를 사용하게 되는데 초기 극심한 통증 제어 및 치료에 활락탕이 유효한 효과가 있었음을 확인하였다. 이에 저자는 본원에 편타성 손상증후군으로 입원하여 활락탕을 복용 한 뒤 호전된 2케이스를 보고하고자 한다. Objectives: The Purpose of this study is to investigate the clinical application of Whallak-tang(Huoluo-tang) to two patients with Whiplash injury. Methods: Patients are hospitalized at Dept. of Oriental Rehabilitation, Bu-Chun Jaseng Oriental Medicine Hospital, diagnosed as Whiplash injury and treated mainly with herbal medine; Whallak-tang(Huoluo-tang). This study was measured by NDI(Neck Disability Index) and VAS(Visual Analogue Scale) score. Results: After taking Whallak-tang(Huoluo-tang), the patient's pain was controlled and slept well after treatment. VAS & NDI score were decreased. Conclusions: As seen in this two cases of whiplash injury, Whallak-tang(Huoluo-tang) has a positive effect to control pain with whiplash injury.

Pyrosequencing을 이용한 전통된장 제조과정 중 세균군집구조의 분석

김용상,정도연,황영태,엄태붕,Kim, Yong-Sang,Jeong, Do-Yeon,Hwang, Young-Tae,Uhm, Tai-Boong 한국미생물학회 2011 미생물학회지 Vol.47 No.3

전통 방식으로 된장을 만드는 과정 동안 세균군집의 다양성과 변화를 관찰하기 위하여 16S rRNA 유전자 서열을 기반으로 하는 pyrosequencing을 수행하였다. 전통 된장 제조에 가장 중요한 접종원으로서 볏짚에 존재하는 세균 군집을 문수준에서 확인했을 때, 상대적 군집 비율로 1% 이상의 분포를 보였던 4종류는 Proteobacteria (71%), Actinobacteria (20.6%), Bacteroidetes (4.2%), Firmicutes (1.3%) 문이었다. 그러나 볏짚 세균 군집구조 결과와 달리 메주의 군집구조에서는 99.1%가 Firmicutes 문이었다. 문 수준에서 숙성 전 된장의 군집분포를 보면 Firmicutes 문 비율이 99.85%로 메주와 비슷한 수준이었다. 그러나 종 수준의 군집구조에서는 메주에서 32.54%의 가장 높은 군집빈도를 보였던 Bacillus siamensis는 0.1%로 거의 사라진 반면 B. amyloliquefaciens가 63.64%로 가장 높은 우점종이 되었다. 숙성 후 된장의 세균군집구조를 보면 숙성 전에 비해 Bacillus 비율이 증가되었으며 이들 중 군집의 상대밀도가 가장 높았던 우점종은 B. amyloliquefaciens (67.3%)였고, 5위까지 모두 Bacillus 종들(전체 군집분포의 92.2%)이 차지했다. 또한 메주 내 상위 11 위까지 우점을 이루던 세균 종들 중 10종이 숙성 후 된장에서도 우점종을 형성하여, 메주 미생물들이 숙성 후 된장 발효까지 영향을 준다는 것을 보였다. 이 결과들로부터 전통 장류에서 발효 주 세균은 Bacillus 종이며 이들은 기본적으로 볏짚으로부터 기원되어 메주에서 우점종을 형성한 것으로 추정되었다. 따라서 풍미와 위생성이 동시에 요구되는 전통 장류의 제조를 위해서는 볏짚 표면에 이 기능을 가진 Bacillus 종들의 군집 분포가 필요할 것으로 예상되었다. In order to evaluate the diversity and change of bacterial population during the manufacturing process of traditional soybean paste (doenjang), bacterial communities were analyzed using 16S rRNA gene-based pyrosequencing. In rice straw, the most important inoculum source for fermentation, the bacterial sequences with a relative abundance greater than 1% were assigned to four phyla, Proteobacteria (71%), Actinobacteria (20.6%), Bacteroidetes (4.2%), and Firmicutes (1.3%). Unlike bacterial community composition of rice straw, a different pattern of bacterial population in meju was observed with predominantly high abundance (99.1%) of Firmicutes. Phylum composition in young doenjang was almost same as that of meju. Major genera in young doenjang were Bacillus (81.3%), Clostridium (6.9%) and Enterococcus (6.3%) and the predominant species among bacterial population was B. amyloliquefaciens (63.6%). Abundance of the phylum Firmicutes in mature doenjang was 99.98%, which was even higher value than those in meju and young doenjang. Predominant species in mature doenjang were B. amyloliquefaciens (67.3%), B. atrophaeus (12.7%), B. methylotrophicus (6.5%), B. mojavensis (3.2%), and B. subtilis. (2.5%), which were also identified as major species of the microbial flora in meju. These results suggested that rice straw was a primary source for supplement of Bacillus species in manufacturing the traditional doenjang and that some species of Bacillus strains were mainly involved in the fermentation process of traditional doenjang.

Poster Session : PS 0077 ; Cardiology : Blood Pressure Changes in Patients Who Underwent Pacemaker Implantation

( Young Ah Park ),( Jae Sun Uhm ),( Tae Hoon Kim ),( Han Cheol Lee ),( Jun Beom Park ),( Jin Kyu Park ),( Bo Young Joung ),( Hui Nam Pak ),( Moon Hyoung Lee ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: In patients with sick sinus syndrome (SSS) or atrioventricular block (AVB), hypertension can be uncontrollable due to the compensatory increase of sympathetic tone. The aim of this study was to elucidate changes of blood pressure (BP) before and after pacemaker implantation. Methods: We retrospectively reviewed the medical record of patients who underwent pacemaker implantation from January 2010 through December 2013 in one center, who were diagnosed with hypertension and taking antihypertensive drugs before pacemaker implantation. Results: A total of 58 patients met the eligible criteria, and they showed signifi cant decreases of systolic BP (143.5±17.3mmHg vs 127.1±13.7mmHg, p<0.001) and mean BP (120.0±14.1mmHg vs 69.4±12.1mmHg, p=0.001) after 3 months of pacemaker implantation. The systolic BP of 36 patients (68.4%) and mean BP of 34 patients (59.6%) were decreased after 3 months of implantation compared to the initial systolic BP and mean BP. The tendency of BP decrease were more obvious in patients with borderline to high initial systolic BP. However, the BP in normotensive patients were increased over time (% of patients with decreased BP, 31.8% vs 82.9%, p<0.001 after 3 months). The reduction of BP in patients with borderline and high initial systolic BP persisted after 9 months of follow up compared with baseline BP (Difference of systolic BP, 22.1 ± 3.7mmHg, p<0.001, difference of mean BP, 15.7 ± 3.0mmHg, p<0.001). The fi nding was independent of the cause of pacemaker implantation (SSS vs AVB 54.8% vs 73.1%, p=0.179 at discharge, 58.1% vs 61.5%, p=1.00 after 3 months). The number of antihypertensive drugs before implantation was not signifi cantly different from that after implantation (before implantation vs after implantation 1.6±0.7, 1.6±0.6, p=0.568). Conclusions: Pacemaker implantation can be helpful for blood pressure control in patients with hypertension and SSS or AVB.

Production of Chimeric Chicken by Direct Injection of Chicken Bone Marrow Cell into Blastoderm

Young Tae Heo,Sung Ho Lee,Yoon Hee Jung,Ji Hoon Yang,Sang Jun Uhm,Teoan Kim,Taek Lee 한국동물생명공학회(구 한국동물번식학회) 2007 Reproductive & developmental biology Vol.31 No.2