Toluene, Xylene, Trichloroethylene 투여가 흰쥐의 시상하부와 뇌하수체의 GnRH, GnRH Receptor, Pit-1 유전자 발현에 미치는 영향

기노석,이종태,강대성,김대환,이채관,이헌,전진호,김정호,이채언 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2

The workplace exposure of chemicals has steadily increased, therefore the concern for subsequent effect on reproductive outcome has been an important issue in occupational medicine. In previous studies, higher rates of spontaneous abortion, reduced fertility and menstrual disorder among women, and an impairement of sperm quantity and quality among men have been associated with a wide variety of chemical agents. This study was conducted to evaluate the effects of toluene, xylene and trichloroethylene(TCE) injection on the mRNA levels of GnRH, GnRH receptor and Pit-1 genes in male rats hypothalamus and pituitary and the effects on the plasma levels of FSH, LH, prolactin and testosterone. Sprague-Dawley male rats were divided into five groups of five each according to concentration of toluene, xylene and TCE. The rats were injected subcutaneously to 0, 50, 100, 200, 400 mg/kg body weight/day of toluene, xylene and TCE, respectively for 6 days. Rat brains were excised and hypothalamus and pituitary were separated. Reverse transcription-polymerase chain reaction (RT-PCR) and RNase protection assay(RPA) were used to evaluate the GnRH, GnRH receptor and Pit-1 mRNA levels. Plasma concentrations of FSH, LH, prolactin and testosterone were assayed by radioimmunoassay(RIA). The results were as follows; 1. GnRH, GnRH receptor and Pit-1 mRNA levels in toluene and xylene injected groups, and GnRH receptor mRNA levels in TCE injected group were lowered dose-dependently. Especially, GnRH receptor and Pit-1 mRNA levels in 200mg/kg of toluene injected group, and GnRH, GnRH receptor and Pit-1 mRNA levels in 400mg/kg of toluene injected group were significantly lower than control group (p<0.05). GnRH receptor and Pit-1 mRNA levels in 400mg/kg of xylene injected group, and GnRH receptor mRNA levels in 400mg/kg of TCE injected group were significantly lower than control group(p<0.05). 2. The plasma levels of prolactin and testosterone in 400mg/kg of toluene injected group, and LH in 100, 200 and 400mg/kg of xylene injected group, and testosterone in 400 mg/kg of TCE injected group were significantly lower than control group(p<0.05). In conclusion, we speculated that toluene and xylene affected reproductive system secondarily through hypothalamus-pituitary axis, and TCE affected directly through steroidogenesis. And we recommended that further study for assessment of the reproductive toxicity of mixed organic solvent exposures should be conducted.

저장 온도 및 상대습도가 비타민 정제 중 지용성 비타민 함량의 변화에 미치는 영향

김동섭,이재황,김세곤,이동언,박석준,이진희,이강표,최성원,백무열 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

청국장 발효에 적합한 미생물의 분리 및 특성

이상원,손미예,조용운,김홍출,갈상완,김철호 진주산업대학교 1999 산업과학기술연구소보 Vol.- No.6

장류발효 식품으로부터 protease 및 amylase 활성이 높은 균주를 중심으로 348 균주를 1차 분리하고, 그 분리균 중 2% skim milk가 함유된 LB평판배지(SM배지)화 2%corn starch가 함유된 LB평판배지(CM배지)에서 halo zone이 크고, 두 효소의 활성이 동시에 높은 SW-251균주를 최종 선별하였다. SW-251균주의 생육 온도 범위는 10∼60℃ 이었으며, 최적 생육온도는 35∼40℃ 이었다. 그리고 최적생육 pH는 6.5 부근이었으나 pH6.0 이하나 pH8.0 이상에서는 생육이 저하하였다. SW-251균주는 호기성의 간균 (0.8∼1.3x1.8∼2.5㎛)으로 운동성이 있으며, 그람양성 반응을 나타냈다. 10% 이하의 NaCl에 내성을 갖고있으며, starch 및 casein을 빠르게 분해하는 세균으로 Bacillus속의 특성을 갖고 있었다. Among 348 strains isolated from fermented soy foods, SW-251 strain has a potent protease and amylase activities on SM medium(LB agar plate supplemented with 2% skim milk), and CM medium(LB agar plate supplemented with 2% corm starch0, respectively. The optimal temperature and growth temperature range for cell growth were 35∼40℃ and 10∼60℃, respectively. The optimal pH was 6.5 and pH range of its growth was 6.0 and 8.0. The isolate was an aerobic bacterium (rod type, 0.8∼1.3 x 1.8∼2.5㎛), then there was its mobility, Gram staining and hydrolysis of starch and casein were positive reaction. Salt tolerance was below 10% NaCl. The strain was identified as a Bacillus sp. SW-251 with respect to morphological, cultural and physiological characteristics.

재조합 균주 Bacillus subtilis가 생산하는 Strept-omyces albus KSM-35 Amylase의 정제 및 특성

이재우, 위성언, 윤옥현 김천대학교 1997 김천대학교 논문집 Vol.18 No.-

Amylase encoded by the samy gene of Streptomyces albus KSM-35 was produced from Bacillus subtilis LKS88 carrying a recombinant plasmid PASA240. The amylase was purified 14.6-folds to homogeneity by salting out with ammonium sulfate, and by some chromatographic procedures using DEAE-Toyopearl, Sephadex G-75, and hydroxyapatite. The molecular weight of purified enzyme was estimated to be 50.000 by SDS-polyacrylamide gel electrophoresis analysis. The optimum temperature and pH of the amylase was 500 and 6.0, repectively. The amylase activity was stimulated by the addition of Na+ ion, whereas it was inhibited by 10 mM Al3+, Cu2+ ions, and 1 mM EDTA. Enzyme inhibitors, such as the ß-mercaptoethanol, concanavalin A, hydoroxylamine-HCl, and acetic anhydride, did not affect the amylase activity. Maltotetraose was detected as the major product during the soluble starch hydrolysis, and small amounts of maltopentaose. maltotriose, and maltose were also found. The general properties like pH, temperature dependences, and starch hydrolysis patterns of Bacillus-expressed amylase maintained the original properties of the s albus KSM-35 amylase.

一部 綜合病院의 診療時間外 應急室利用患者 實態調査

李常鏞,沈雲澤 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.1

We analyzed the 476 patients who came to the emergency rooms of 5 general hospitals in Tae-jeon city in the all hours except the the regular duty hours for a week during the period from July lst, 1986 to July 31st, 1986, on the basis of general characteristics on the injury, the time of occurrence, causes of incidence, degree of injury and the department where they were treated. We came to the following results: 1. The sex rate is male, 54.0% feamale, 46. 6%. In terms of age rate, the group(20-29) showed the greatest percentage, 24.6% and the group(50-59) showed the lowest percentage. According to the occupation, the housewife group showed the greatest rate(19. 1%), and the student group occupied the 2nd standing (18.3%) and the preschool children group occupied the 3rd standing(15. 0%). 2. The incidence hour of emergency patients who came to the emergency room in the hours except the time of regular diagnosis and treatment showed the following standing to the classification of the day of the week. Incidence rate is the highest between 18:00-24:00 and 24:00-04:00 in saturday and 21:00-24:00 in sunday. 3. The time required for the patients to go into the hospitals showed that it is less than 1 hour 37. 6%, 1 to 2 hour 21, 9%. About 60% of emengency patients are shown to have entered the emergency room within 2 hours. 4. In terms of the order of the causes of incidence, it showed that disease was 57. 6%, other accidents 16. 2%, traffic accidents 10. 5%, injury 7. 6%, drug intoxication 5. 2%, pregnancy & delivery 2.9%. 5. For the severity of injury, the moderate case was 26.9%, the mild case was 26.7%, milder case was 22.3%, the serious case was 19. 1% and the most serious case was 5. 0%. 6. For the Medical Field of the Patient, it also showed that : Inetrnal medicine 36.8%, General surgery 18. 3%, Neurosurgery 11. 3%, Orthopedics surgery 10. 7%, Pediatrics 6.5%, Obstetrics & gynecology 4. 6%, Dentistry 3. 8%, Opthalmology 2. 3%, Otorhinolaryngology 1. 9%, Dermatology 1.5%.

양파의 채종 기술 개선에 관한 연구 : 4. 화학물질 처리에 의한 환경단축 4. Reduction of Flower Stalks Height by Chemical

李愚升,趙殷衡,藤本幸平 慶北大學校 1989 論文集 Vol.48 No.-

Ethrel 750ppm was sprayed on the leaves of onion at one week interval from just after flower bud differentiation to just after bolting to reduce the flower stalk height. Six concentration levels of ethrel (0, 750, 1,000, 1,500, 2,000, and 5,000ppm) were sprayed on the leaves on April 11. In ethrel 750ppm, bolting percentage was the least in April 18 application at May 3 count followed by April 25 application. Reduction of flower stalk height was maximum at April 18 application in 750ppm followed by April 25, March 24, April 11, May 2, April 4, May 9, March 28 in order. Germination speed and germination percentage were the least in ethrel 750ppm applied on April 18 as compared to control. Bolting was delayed, height of flower stalks was reduced, and germination speed and percentage were decreased with increased concentration of ethrel in the range of over 1,000ppm. Foliar application of ethrel 750ppm or lower just after bolting would be beneficial in reduction of flower stalk height in practice.

대전·충남지역 와병노인의 실태에 관한 연구

이동배,심운택,이태용,오장균,정성훈 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

This study was conducted to investigate the prevalence rate and the relationship between causative diseases and self-help abilities in bed ridden patients of the elderly. The study group was composed of 1.568 peoples aged 65 or more, living in Taejeon city and Chungnam provincial area. The results obtained were as follows : 1. The prevalence rate of bed ridden patients were 5.7% in male, and 4.3% in female. The rates revealed increasing basis with the ages. 2. The prevalence rate was higher in city area with 6.1% than inland and seaside area with 4.3%, 3.7% respectively. 3. The mean age of bed ridden patients were 75.9 years in male. 78. 0 years in female. The rates were variable with residential area. As the cause of bed ridden patients the cerebrovascular disease was higher in urban than rural area. 4. The mean age of causative diseases was the highest in contusion and fracture with 82.8 years. The period of bed ridding was the highest in muscular disease and arthropathy with 60.2 monthes. 5. The average score of activity of daily living was the highest in muscular disease and arthropathy and followed by respiratory disease, other disease, senility, cerebrovascular disease, contusion and fracture with decreasing basis. But most bed ridden patients did the activity of daily living similar to those of normal persons. 6. The causative diseases were diagnosed mainly in general hospital, hospital. and herb medicine. but muscular disease and senility were in self-diagnosed. 7. The helper of bed ridden patients were mainly wives or daughters in law.

Rhizopus japonicus와 Zymomonas mobilis의 혼합 고정화 배양계를 이용한 생전분으로부터 비살균처리에 의한 에탄올 발효

이상원,조용운 진주산업대학교 1998 論文集 Vol.37 No.-

Rbizopus japonicus와 Zymomonas mobilis로 구성된 혼합고정화 배양계(R-Z 24계)의 특성을 이용하여 biomass로부터 유용물질 생산을 행할 때 투입되는 에너지를 절약할 목적으로 비가열살균 배양을 검토하였다. R-Z 24계 만으로의 비가열살균 배양은 배지에 함유된 세균 때문에 어려웠다. Sorbic acid, benzoic acid, dehydroacetic acid, P-hydroxybenzoic acid와 Vantocil IB등의 여러 가지 살균제 중에서 0.1% Vantocil IB가 잡균의 생육을 억제하는데 가장 적합하였다. Vantocil IB는 곰팡이보다 세균에 대하여, 또 고정화 배양보다 액체배양에서 더 강한 효과를 나타내었다. 0.1% Vantocil IB를 첨가한 비가열살균 배양에서 2, 5 및 10%의 생전분 기질로부터 각각 6.5g/ℓ(Yp/s=0.38), 14.5g/ℓ(Yp/s=0.36) 및 28g/ℓ(Yp/s=0.33)의 에탄올이 생산되어 살균제를 첨가하지 않은 대조구와 동등한 수율을 얻었다. In order to reduce energy input in direct ethanol production from raw starch without sterilization, (co-immobilized mixed Rbizopus japonicus and Zymomonas mobilis culture system(R-Z 24) which changed to anaeoric from aerobic condition after 24hrs of fermentation was investigated.) R-Z 24 system can not be used for ethanol production because of growth of microbial contaminants from medium under nonstrile condition. 0.1% Vantocil IB was the most effective food additive to suppress the growth of contaminants among sorbic acid, benzoic acid, dehydroacetic acid and p-hydroxybenzoic acid. Microbial growth inhibition of vantocil IB was more effective in a bacteria than in a mold, and also in free culture system than immobilized culture system. By adding 0.1% Vantocil IB to the fermentation medium without sterilization, ethanol production was 6.2g/ℓ (Yp/s=0.35), 14.5g/ℓ (Yp/s=0.36) and 28g/ℓ(Yp/s=0.31) from 2, 5, 10% untreated raw starch respectively. These productivities were similar to those of control culture system without Vantocil IB.

전력 송·배전 시스템 상에서 발생하는 이벤트에 대한 데이터 마이닝 가상 트랜잭션과 연관 규칙의 적용

이준섭,이창언,윤상일,김응모 成均館大學校 科學技術硏究所 2002 論文集 Vol.53 No.1

There has been much research on data mining techniques for applying more advanced applications. In this paper, we introduce a approach to convert time series data into virtual transaction data for analysis power electronic transmission and distribution system. However, most of these techniques has focused on transaction data rather than time series data. We present a new way which is discovered and repaired faults of Electric Power system using Data Mining techniques.

패각분말의 소성가공 특성에 관한 연구

이상은,오성규,김종오 경상대학교 환경보전연구소 2000 環境保全硏究所報 Vol.8 No.-

The objective of this study was to investigate the proper calcination conditions of powdered oyster shell. The physical and chemical characteristics such as particle size distribution. calcium contents, pH, and CEC(Cation Exchinge Capacity) were also examined. The following results were obtained. 1. As the powdered oyster shell was ignited in high temperature of 650℃ to 950℃, the calcination reactions was effectively progressed. The amount of weight loss during calcination would be matched with the generation of carbon dioxide gas. 2. In the calcination of powdered oyster shell. the amount of weight loss increased according to the increase of calcination temperature and retention time. It was founded that the proper temperature and retention time of calcination was 850℃ and 40 minute.