Effects of dietary supplementation with probiotic CS-A on performance in broiler chickens

Seong Soo Kang1*, Se Eun Kim, Ara Go, Kyung Mi Shim, Chun Sik Bae, Chang Jong Moon, Sung-Ho Kim, Jong-Choon Kim, Jin-Cheol Yoo, Seung Sik Cho 충북대학교 동물의학연구소 2012 Journal of Biomedical and Translational Research Vol.13 No.3

Probiotics, enzymes, organic acids, oligosaccharides, antioxidants, and other functional materials are actively being explored as alternatives to antibiotics. Probiotics include live beneficial microorganisms that colonize the intestinal tract and competitively inhibit attachment and growth of harmful microbes. Probiotics also increase feed efficiency by assisting in nutrient absorption and digestion. The current study was conducted in order to evaluate the effect of a new probiotic, CS-A, as a dietary supplement of a fermented product on growth performance, feed intake, and feed conversion efficiency in broiler chickens, and to evaluate its value as an alternative for antibiotics used as a feed additive. Antibacterial and anti-inflammatory effects of CS-A were investigated in vitro and the in vivo effects of a constant concentration of supplemented CS-A on growth rate and feed efficiency were evaluated. In addition, the safety of CS-A was assessed by examination of common symptoms and mortality. Determination of minimal inhibitory concentration revealed an excellent antibacterial effect of CS-A. Cytotoxicity was low and anti-inflammatory effects were achieved at the effective concentration of CS-A. Supplementation with 0.1% CS-A resulted in a feed efficiency score of 1.84 in broilers, compared to 2.00 in the control group. There were no adverse clinical findings, necropsy findings, hematology, and altered serum biochemistry parameters, and no mortality. Thus, it is concluded that CS-A is safe and effective as a feed additive.

數學의 槪念形成을 爲한 敎授·學習模型의 硏究

姜慶愛,申澤均,金大洙 대구교육대학교 1975 論文集 Vol.11 No.-

This study is attempted to develope a better way of forming the concepts of mathematics in teaching and learning. A lot of basic concepts of mathematics have been mostly instructed in the primary school in such a way as verbal explanation. Therefore, no opportunity has ever been given to pupils for their logical, rational way of thinking. Some teaching-learning ways of concepts formation has been already expressed but no teaching-learning pattern as to what steps must be taken for their instruction has been yet found. This paper aims at developing such a pattern and helping the inservice teachers apply it for the improvement of mathematics education. The concepts to be instructed in the primary school are divided into three-objective, operative and relative. As they are considerd to be taught in different ways, the fellowing patterns are made. The objective concepts are to be instructed in such a way as ① pattern presentation, ② comprehension as a set, ③ concepts building, ④ definition of concepts ⑤ application; the operative concepts as ① motivation, ② comprehension as a set, ③ concepts building, ④ being made into notation, ⑤ training for skill, the relative concepts as ① case presentation, ② comprehension as a set, ③ concepts building, ④ being made into notation, ⑤ application. According to this, <prism> was actually instructed in the sixth grade of primary school for the understanding of objective concepts; <adding> in the first grade for operative concepts; <perpendiculum> in the fourth grade for relative concepts. The results showed easy and correct understanding as well as logical and rational thinking on the part of school boys and girls. The assumption that the process of forming the three patterns may be different was wrong and a general model was mode: model presentation→comprehension as a set→concepts building→being made into notation→application. All that is needed is that the teaching method of each step should be different.

포자배양에 의한 박쥐란 (Platycerium bifurcatum (Cav.) C. Chr.)의 대량증식과 순화재배에 관한 연구

姜聲述,梁會亨,曺敬喆,金光秀 全南大學校 農業科學技術硏究所 1998 農業科學技術硏究 Vol.33 No.-

박쥐란(Platycerium bifurcatum (Cav.) C. Chr.)의 기재 대량 증식과 순화배지 개발을 목적으로 포자발아에 적합 배지, 유식물의 발근율 향상을 위한 생장조정제 종류 및 활성탄소의 농도를 구명하고 훈탄, 수태, 바크와 왕겨, 피트와 퍼라이트 및 버미큐라이트와 퍼라이트 등 몇가지 재식 재료의 종류가 박쥐란 유묘의 생육에 미치는 영향에 관하여 알아 보고자 실험은 실시하였다. 포자의 발아는 agar 보다 gelrite 첨가배지에서 좋았다. 포자체의 성장에는 MS 배지 보다 hyponex, Knudson 배지가 좋았다. 생장조정제 종류의 영향에 있어서는 shoot 발생에는 IAA와 NAA 첨가배지에서 양호했고 rooting은 NAA 첨가배지에서만 발생하였다. 활성탄소의 종도에 따른 shoot 발생과 지상부생육은 0.3%에서 가장 양호했고 뿌리수도 같은 경향으로 적정 농도는 0.3∼0.6%였다. 특히 활성탄소의 농도 그 이상인 경우는 근부 발달이 현저히 억제되었다. 유묘의 순화는 훈탄과 수태단용, 피트 + 퍼라이트에서는 신초수, 지상부 및 지하부 생육이 양호한 반면 바크와 왕겨 혼합용토는 식재재료로 부적당하였다. This study was carried out to establish a mass-propagaion system of Platycerium bifurcatum (Cav.) C. Chr. through in vitro culture of spores. Effects of growth regulator and activated charcoal on the in vitro plant development from spores were examined, and an ideal potting material for acclimatization of the sporelings was developed in this experiments. Gelrite was better than agar as gelling agent of the media for spore germination. The gametophytes grew better on hyponex or Knudson medium than on MS medium. The culture medium with IAA and NAA was better for the plant shooting and rooting than the medium containing NAA only. Multiple shoot formation as will as shoot and root growth was promoted by the addition of activated charcoal aat 0.3% to the culture medium, byt, the plant growth, especially root development was retarded when the concentration of charcoal exceeded 0.6% in the culture medium. Carbonized rice hull, sphagnum moss or peat with perlite in pot mixture resulted in the desirable shoot and root growth.

세 학교의 통합교육 운영 사례

강경숙,권택환,김수연,김은주 국립특수교육원 2000 통합교육 시범학교 운영사례집 Vol.2000 No.-

통합교육은 특수교육의 중요한 철학이자 목표일뿐만 아니라 교육현장에서, 매일의 수업 및 생활장면에서 장애아동이 일반학급의 한 구성원으로 또래와 함께 적극적인 참여자가 되기 위한 지원을 제공하는 노력의 과정 그 자체라고 할 수 있다. 지금까지 우리나라의 실정에 적합한 통합교육의 이론이나 모형 개발, 혹은 구체적인 교수방법에 대해서 적지 않은 연구가 이루어졌으나 학교 현장에서 이루어지고 있는 통합교육의 실제를 통해 성공과 실패 요인들을 분석해내는 연구는 부족하다. 이에 본 연구는 선진 외국의 사례나 이론에 맞추어서 바람직한 통합교육의 모습을 알리고 실행방법을 보급하는 것이 목적이 아니라 우리 나라 상황에서 실제적으로 이루어지고 있는 통합교육 현장의 실제를 깊이 있게 분석하고자 하였다. 일반학교에서 이루어지고 있는 매일의 수업과 생활장면에서 통합교육이 어떻게 이루어지고 있는지 살펴보고, 이를 통해 바람직한 사례를 확산시키고, 제대로 이루어지지 않는 사례에 대해서는 그 이유와 원인을 분석하여 제언하고자 하였다. 본 연구의 대상은 서울의 공립초등학교 2개교와 경기도의 공립초등학교 1개교로 모두 세 학교의 통합교육 사례를 연구하였다. 한 명의 장애아동을 중심으로 통합학급과 특수학급을 오가면서 세 명의 연구자가 수업을 참여관찰하고 교사 및 아동들과 면담을 실시하였다. 참여관찰 기간은 2000년 4월 10일부터 9월 9일까지로 일주일에 1회, 총 12회 이상 방문하였다. 연구대상 학교의 모든 일반교사와 일반아동, 일반아동의 학부모, 장애아동의 학부모를 대상으로 깊이 있는 면담을 실시할 수는 없었으므로 통합교육에 대한 설문조사를 실시하였으며 각종 서류와 문서들을 내용분석하여 자료를 수집하였다. 참여관찰 및 면담, 설문분석과 내용분석을 통해 세 학교에서 얻은 자료를 바탕으로 연구자간 협의를 거쳐 다음의 네 가지 영역으로 연구 결과를 분석 기술하였다: (1) 통합환경에서의 교육과정 운영; (2) 통합환경에서의 사회적 상호작용; (3) 통합교육을 위한 부모 및 가족 지원과 협력관계; (4) 행정적인 지원 및 지역사회의 연계를 비롯한 여건 조성. 본 연구결과를 요약하면 다음과 같다. 첫째, 통합환경에서의 교육과정 운영 면을 살펴보면, 특수학급에서는 특수학급 고유의 교육과정을 운영하고 있었으며, 이 중에는 기능적인 교육과정의 내용이 포함되어 있었다. 특수학급에서의 수업뿐만 아니라 일반학급에서의 수업을 지원하는 것에 대해 특수교사, 일반교사, 부모 모두 관심을 가지고 있었으나 실제로 시행되기에는 어려움이 있었다. 부분적이나마 장애아동이 일반학급에서 받는 수업을 지원하기 위해 크고 작은 시도를 하고 있었는데, 세 학교 모두 특수교사와 일반교사의 협력문제에 대해 어려움을 느끼고 있는 것으로 나타났다. 마지막으로 장애아동이 일반학급에서 받는 수업의 질을 향상하기 위해 요구되는 제도적인 지원들에 대한 논의가 이루어졌다. 둘째, 통합환경에서의 사회적인 상호작용 면을 살펴보면, 통합교육이 비교적 활발히 시행되고 있는 학교를 대상으로 하였으므로 세 학교 모두 장애아동이 일반학급에서 긍정적으로 수용되고 있었으며, 일반교사와 특수교사 모두 장애아동의 사회적인 통합을 위해 다양한 시도를 하고 있었다. 다만 장애아동을 도와주는 친구들은 많이 있으나 동등한 위치의 친구라기보다는 도우미, 혹은 또래 교사로서 도와주는 방법에 더 익숙한 모습을 발견할 수 있었다. 장애아동의 사회적 통합을 촉진하기 위해 이루어지는 교사의 지원과, 또래와의 상호작용을 강화하기 위한 대안들이 제시되었다. 셋째, 부모 및 가족과의 협력관계를 보면, 장애아동의 부모는 통합교육을 간절히 원하고 많은 노력을 기울여 자녀를 통합장면에 배치시키는데 이러한 과정에서 마음의 고충을 많이 겪고 있었다. 장애아동의 부모는 우선적으로 일반아동들과 일반교사들의 장애아동과 통합교육에 대한 인식의 부족이 가장 큰 문제라고 지적하였으며, 일반아동의 부모와 우호적인 관계를 맺기를 원하였다. 장애아동의 부모가 자녀의 통합교육을 위해 적극적으로 활동하는 경우에는 부모들간의 모임을 통해 실패의 경험과 정보를 공유하면서 교사와 협력하여 통합교육의 질을 높이기 위해 노력하는 모습도 볼 수 있었다. 넷째, 행정적 지원 및 전반적인 여건 조성 면에서는 세 학교 중에서 비교적 효율적인 통합교육의 실행에 필요한 행정적인 지원이 제도화되어 있는 한 학교를 중심으로 방법이 설명되었다. 통합교육이 교사 혼자의 의지와 노력에 좌우되는 것이 아니라 행정적으로 지원해줄 수 있는 제도적인 장치의 필요성이 강조되면서 특히 학교장의 역할이 중요한 것으로 제기되었다. 본 연구결과에 의하면 통합교육의 성패에 영향을 미치는 요인에는 여러 가지가 있겠지만 특수 교사와 일반교사의 긴밀한 협력관계가 특히 중요한 것으로 나타났다. 또한 본 연구에서 대상으로 한 세 학교 모두 특수교사나 일반교사가 통합교육의 성공적인 실행을 위해 많은 노력과 다양한 시도를 하고 있기는 하나, 수업 참여를 위한 교수방법이나 또래 교수 혹은 우정을 형성하고 지원하기 위한 전문적인 지식과 프로그램에 대한 자원들이 필요한 것으로 나타났다. 앞으로 본 연구결과를 바탕으로 통합교육의 성공적인 실행을 위한 다양한 지원체계가 갖추어져야 할 것이다. Inclusion is not only the main philosophy and purpose of special education but also the process of supporting full participation of students with disabilities in every aspect of daily and school life. As the number of special classes increased rapidly in late 80s, inclusion became a central topic in research and practice. In late 90s, the discussion was developed into the application of more concrete strategies for successful inclusion. The quality of inclusive education also began to draw attention. However, there has not been enough research about the facilitating factors and impeding factors of inclusive education based on the analyses of the existing practices of inclusive education conducted in real school settings. Therefore, the purpose of this study is to investigate what is really going on in the classes and schools where children with special needs are included. Rather than aiming to disseminate the desirable aspects of inclusive education in developed countries, this study was designed to analyze the current practices of inclusive education in Korea, address the facilitators of inclusion based on exemplary cases, and make suggestions to remove impeding factors. Two public elementary schools in Seoul and one public elementary school in Kyunggi-do are the main sites of the study. Over a period of time between April 10th and September 9th, the three researchers observed a student with disabilities in each site both in his or her self-contained and integrated settings. For the data collection, the researchers visited the sites once a week, at least 12 times for each school. Since it was unrealistic to interview all the general education teachers, the students without disabilities and their parents, and parents of the students with disabilities in the three schools, a questionnaire survey was conducted to investigate their perceptions of inclusive education and the students with disabilities. Also, content analysis was conducted on the documents gathered to examine overall state of managing inclusive education. Findings are organized into four themes that emerged from the analysis of the data from participant observation, interviews, a survey, and document reviews: (a) curriculum management in inclusive settings; (b) social interactions in inclusive settings; (c) collaboration with or supports from parents and families; and (d) administrative support and connection with communities as a foundation of inclusion. First, regarding curriculum management, the study found that special education classes had unique curricula of their own which included functional curriculum. The general education teachers, special education teachers, and parents of students with disabilities in the study expressed their needs for supports both in self-contained and integrated educational settings. The special and general teachers in the study made their own efforts to facilitate inclusion, but they indicated that collaboration between special and general education teachers was not easy. The supports at the system level to promote the quality of education that students with disabilities receive in inclusive settings are also discussed. Second, regarding social interactions, the study found that students with disabilities were accepted positively in inclusive settings. Both special and general education teachers made a variety of efforts to promote social integration of the students with disabilities, though it was partly because the three sites for the study were the schools where inclusive education had been administered quite actively. On the other hand, the study found that many students without disabilities were accustomed to helping the students with disabilities as a helper or a peer-tutor, rather than as a true friend. In this section, implications for teachers are provided regarding how to facilitate peer interactions between students with and without disabilities. Third, regarding the collaborative relationships with parents and families, the study found that the parents of the students with disabilities eagerly wanted their children to be included in the general classes and experienced emotional difficulties in the process of trying to make the inclusion work. The parents indicated that the level of acceptance by general education teachers and students without disabilities had been the biggest issue in inclusive education. The parents also expressed their desire to establish collaborative relationships with parents of students without disabilities. Especially, those parents who actively participated in the process of inclusion supported other parents of children with disabilities by sharing information, concerns, and experiences through parent group meetings. Fourth, in terms of administrative supports and overall foundations for inclusion, findings are described based on the one school where the level of the administrative supports was exemplary. Since successful inclusion cannot be achieved by the willingness and efforts of a single teacher, the roles of principals were emphasized. Though there would be so many factors that are influential in determining the success of inclusive education, the study found that collaboration between special education teachers and general education teachers was the most essential factor. These stakeholders should work in partnership through active communication, information exchange, and mutual supports in order to enhance the quality of education and social interactions that the students with disabilities experience in inclusive settings. Though various efforts have been made to realize successful inclusive education in the three schools, there was a need for (a) more information regarding instructional strategies, peer tutoring methods, and facilitation of friendships, and (b) resources to develop programs in these areas. Based on the findings of the study, more comprehensive systems of support should be established.

점탄성 감쇠기의 최적설계

강경수 동명정보대학교 2002 건축도시연구소 저널 Vol.1 No.-

The purpose of this study is to present the design method of visco-elastic dampers. The dynamics of visco-elastic dampers and support braces connected in series is modeled by state equation. A constraint on maximum story drifts which are computed using RMS's of story drifts and peak factors is added to the optimization problem. The number of variables is reduced by including the constraint associated with the dynamic behavior of the structure in the procedure to compute the gradient of the inequality equation about constraint on the maximum story drifts. In the design example, it is confirmed that the design of dampers considering support brace stiffness is necessary when sufficient brace stiffness can not be supplied. It is also found that unnecessary brace stiffness can be removed by adding brace stiffnessess to optimal design variables and that the increase of damper volumes to compensate for the variation of maximum story drift is pretty small.

전치의 후방견인에 따른 입술과 주위 연조직의 수직적 변화

강창수,김경호,최광철 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.2

입술의 돌출을 해소하고자 하는 환자들은 자신의 안모 개선을 안모의 측면보다는 거울로 볼 수 있는 정면을 통하여 느끼는 것이 보편적이다. 안모의 정면에서 평가가 이루어질 때에는 입술이 후방으로 들어간 정도와 함께 입술과 그 주위 조직의 수직적 변화에 의해서도 큰 영향을 받을 수 있으므로 교정치료 진단이나 치료 후 평가를 위한 측모두부방사선 사진 분석 시 전통적으로 중요시되고 있는 입술의 시상적 이동뿐만 아니라 흔히 간과하기 쉬운 수직적인 변화에 관해서도 평가가 이루어져야 한다. 따라서 본 연구에서는 입술 돌출을 주소로 내원한, 성장이 완료된 성인 여성 61명을 대상으로 측모두부 방사선 사진을 촬영하여 전치의 후방견인시 나타나는 입술과 주위 연조직의 수직적 변화를 계단식 다중 회귀 분석법을 이용하여 조사한 결과 다음과 같은 결론을 얻었다. 1.상, 하순의 philtrum길이 (SnLs, LiB') 는 전치의 후방견인 후 증가하였고, 상, 하순의 vermilion height (LsStms, StmiLi) 와 vermilion length (LsLi) 는 감소하였다. 2.상, 하순의 길이 (SnStms, StmiB')와 연조직의 하안면 고경 (SnMe')은 치료 후 유의성 있는 변화를 보이지 않았다. 3.상순의 philtrum길이 증가에 상악 전치의 정출 △UIV 이 가장 큰 영향을 주었으며 하순의 philtnlm길이 증가에는 치료 전 overjet이 가장 큰 영향을 주었다. 4.상, 하순의 vermilion height감소에 상순의 두께 감소가 가장 큰 영향을 주었다. Patients who want to reduce their lip protrusion usually estimate the severity of the lip protrusion on the frontal aspect. Most orthodontists have a perplexed experience of a reduced thin line of vermilion border on the frontal aspect as incisors are retracted, even though the lip protrusion is thought to be reduced favorably on the sagittal aspect. Some patients also look older after orthodontic treatment because of severe lip thinning. This unaesthetic reduction of vermilion border urges us to study the vertical lip change during orthodontic procedure. The purpose of this study was to evaluate the vertical lip and perioral soft tissue changes in respect to incisor retraction in an effort to analyze which factors might be responsible for their vertical changes, using the multiple regression analysis. The results were as follows. 1.Upper and lower lip philtrum length(SnLs, LiB') were increased after retraction of anterior teeth, where as upper and lower vermilion height(LsStms, StmiLi), and vermilion length(LsLi) were decreased. 2.Upper and lower lip length(SnStms, StmiB'), and soft tissue lower anterior facial height(SnMe') did not show any significant difference after treatment. 3.The increase of the upper lip philtrum length was mainly influenced by the extrusion of upper anterior teeth(△UIV), and the increase of the lower lip philtrum length was mainly influenced by the initial overjet before treatment. 4.The decrease of the upper and lower lip vermilion height was mainly influenced by the decrease of upper lip thickness.

축소모드해석방법을 이용한 복수동조질량감쇠기의 진동제어 효과에 관한연구

강경수 동명정보대학교 2000 東明情報大學校論文集 Vol.3 No.-

In this study the vibration control effects of tall building with MTMD has been analyzed using a reduced modal analysis method. The tall buildings have many fundamental modes, but the dynamic behavior of Tall buildings is governed by a few modes. And the buildings have many different sections are governed by not the first fundamental mode but the other modes. The reduced modal analysis method is that the modes has almost governed the dynamic behavior of the structure are considered in dynamic analysis of the structure with many degrees of freedom. This method is more reliable and simply analysis of tall building with MTMD than other methods. Two analytical models is shown the vibration reduction effects of tall building with MTMD by the reduced modal analysis method.

자폐아동에서 FMR-1 유전자에 대한 분자유전학적 연구

강경미,곽동일,이민수 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.6

연구목적: 자폐장애가 유전성이 있다고 제시하는 많은 쌍생아 및 가족연구와 유전자 연구에도 불구하고 이 질환의 유전방식과 관련 유전자는 현재까지 알려져 있지 않고, 이 장애는 임상적으로 이질적 장애이며 유전방식과 관련 유전자는 현재까지 알려져 있지 않고, 이 장애는 임상적 이질적 장애이며 유전적으로도 다인자 질환일 가능성이 높은 것으로 생각된다. 본 연구에서는 자폐장애와 FMR-1 유전자간의 연관성을 조사하고자 자폐장애 아동 총 66명을 대상으로 분자유전학적 검사를 시행하였다. 방 법: 연구대상군은 DSM-Ⅳ 진단기준으로 자폐장애가 해당하고 K-CARS 점수가 30점 이상인 아동들을 대상으로 하였다. Genomic DNA를 전혈에서 추출한후, 탐침 Xho/PstⅠ과 두 개의 제한효소(EcoR Ⅰ, Eag Ⅰ)를 사용하여 썼던 블롯팅을 시행하였다. 결 과: 연구대상 총 66명중 1명의 남아가 메칠화된 모자이크의 양상을 보이었고, 3명의 남아와 2명의 여아는 불완전 변이 양상을 보이었으나 완전 변이의 양상은 없었다. 결 론: 본 연구에서는 FMR-1 유전자가 자폐장애의 유전적 요인과 관련이 있다는 이전의 보고를 뒷받침할만한 명백한 결과를 얻지 못하였다. Objectives: To elucidate an association of the fragile X syndrome with autism, Southern blot analysis was performed in 66 autistic children aged from 2 years to 11 years. Methods: Subjects were 66 autistic children with of autistic disorder diagnosed by DSM-Ⅳ criteria and Childhood Autism Rating Scale-Korean version. Genomic DNA was extracted from peripheral blood and DNA was used to detect a FMR(Fragile Mental Retardation)-1 gene. Xho/PstI probes and two restriction enzymes(EcoRI, EagI)were used for Southern blot analysis. Results: There were one boy with a methylated mosaic pattern and 3 boys and 2 girls with an unmethylated premutation band. But there was no full mutation pattern. Conclusion: Although the possibility of the relationship between autistic disorder and FMR-1 gene has been suggested, the results from this study do not provide any definite association of FMR-1 gene with autism in autistic children.

차량용 와이퍼 시스템 프레임의 형상 최적설계

강경수,이종호,우호길 충남대학교 공업교육연구소 2002 論文集 Vol.25 No.1

In this study, a shape optimization for a frame of a vehicle windshield wiper system is presented. This study investigated how to optimize a shape and minimize mass(volume) and the number of parts with sufficient strength of a frame instead of a conventional structure. The dffectiveness of this analysis procedure is expected to reduce development lead time &cost actually.

떫은감 Carotenoid의 색소 안정성

강미정,윤경영,성종환,이광희,김광수 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.4

Studies on extraction and color stability of carotenoids from astringent persimmon(Diospyros kaki) were performed to provide the basic information for the utilization of persimmon pigment as a new source of natural food colorant. The major carotenoids in astringent persimmon were beta-cryptoxanthin, lycopene and beta-carotene. Beta-cryptoxanthin was the first major pigment and lycopene the next. Total carotenoid content in persimmon calculated as beta-carotene equivalent was 107.4 ㎍/g. The data indicated that the astringent persimmon was a good source of carotenoid pigment. The physical and chemical stabilities of carotenoid from astringent persimmon were investigated at various conditions of temperature, pH and antioxidant. The effects of oxygen and light on the stability of carotenoid pigment has been investigated. The factors that cause the discoloration were visible light, temperature above 60, pH below 5 and oxygen. Especially, the carotenoid pigment was very sensitive to light and oxygen. Carotenoid stability was much improved with increasing nitrogen concentration in the atmosphere. Persimmon pigments have been found to be most stable at 5~10. And tocopherol was the most effective inhibitor of the pigment discoloration.