Evaluating the effects of honey bee (Apis mellifera L.) venom on the expression of insulin sensitivity and inflammation-related genes in co-culture of adipocytes and macrophages

Kim Hee‐Yeon,Jo Min Jeong,Nam So Yung,Kim Kwang Min,Choi Moon Bo,Lee Yong‐Ho 한국곤충학회 2020 Entomological Research Vol.50 No.5

Obese adipose tissue is characterized by adipocyte hypertrophy and a massive macrophage infiltration. The interaction between macrophages with mature adipocytes releases pro-inflammatory cytokines. This chronic inflammatory state can contribute to obesity-related complications, such as insulin resistance, type 2 diabetes and cardiovascular disease. Therefore, we can attempt to prevent and treat obesity-related diseases by inhibiting macrophage infiltration and blocking their interaction with adipocytes. Honey bee (Apis mellifera) venom (BV) has been reported to have anti-inflammatory effects. Although BV is used to treat chronic inflammatory diseases, few studies have addressed its use in obesity-associated inflammation. This study examines the inhibitory effects of BV on lipid accumulation in differentiating preadipocytes, inflammation, and insulin resistance in macrophages and adipocyte-macrophage co-culture system. We treated 3 T3-L1 preadipocytes with BV during differentiation. We later measured lipid accumulation and gene expression of master adipogenic transcription factors. After RAW264.7 and 3 T3-L1 cells were pretreated with BV, RAW264.7 cells were activated with LPS or co-cultured with pretreated 3 T3-L1 cells. Gene expression of pro-inflammatory cytokines and insulin sensitizing genes was measured in these cells. BV inhibited lipid accumulation and C/EBPα and PPARγ gene expression during intermediate and late 3 T3-L1 cell differentiation. BV also suppressed gene expression of pro-inflammatory cytokines (COX-2, iNOS, MCP-1, TNF-α, IL-1β and IL-6) in LPS-stimulated macrophages, and in co-culture of 3 T3-L1 adipocytes and RAW264.7 macrophages. However, adiponectin and GLUT-4 expression were both significantly increased by BV in co-culture. These findings demonstrate that BV attenuates adipocyte hypertrophy and improves obesity-related inflammation and insulin resistance in obese adipose tissue.

Lactobacillus pentosus 발효에 의한 담쟁이덩굴 줄기 추출물의 항산화 및 세포보호 효과

박소현 ( So Hyun Park ),성준섭 ( Joon Seob Seong ),이건수 ( Keon Soo Lee ),박영민 ( Young Min Park ),현송화 ( Song Hua Xuan ),차미연 ( Mi Yeon Cha ),강희철 ( Hee Cheol Kang ),박수남 ( Soo Nam Park ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.3

본 연구에서는 담쟁이덩굴 줄기 70% 에탄올 추출물과 발효균주 Lactobacillus pentosus를 이용하여 발효시킨 담쟁이덩굴 줄기 발효추출물에 대하여 항산화 및 세포보호 효과를 측정하였다. 1,1-Diphenyl-2-picrylhydrazyl (DPPH)를 이용한 자유라디칼 소거 활성(FSC₅₀)은 담쟁이덩굴 줄기 추출물 및 발효추출물이 각각 42.3 및 34.5 μ g/mL로 발효 후의 라디칼 소거활성이 약 18.4% 더 높게 나타났다. Lumiol-의존성 화학발광법을 이용한 Fe³⁺-EDTA/H₂O₂계에서의 총 항산화능(OSC₅₀) 평가에서도 담쟁이덩굴 줄기 추출물과 발효추출물은 각각 2.6 및 2.5 μ g/mL로 발효 후가 약 4.2% 정도 더 높은 총 항산화능을 나타냈다. ¹O₂로 유도된 적혈구 세포손상에 있어서 추출물 및 발효추출물의 세포 보호 효과(τ₅₀)는 50 μ g/mL에서 각각 126.4 및 173.0 min을 나타내어 발효 후 세포 보호 효과가 약 34.0% 더 높게 나타났다. 발효추출물은 지용성 항산화제로 알려진 (+)-α-tocopherol (43.4 min)보다도 3.9배 높은 세포 보호 활성을 보여주었다. 사람 섬유아세포인 Hs68을 대상으로 elastase 저해 활성을 조사하였다. Elastase 저해 활성(IC₅₀)은 담쟁이덩굴 줄기 추출물과 발효추출물에서 각각 873.6 및 687.8 μ g/mL로 발효 후에 elastase 저해 활성이 약 21.3% 더 높은 것으로 나타났다. 이상의 결과들은 담쟁이덩굴 줄기 발효추출물이 항산화 작용과 더불어 주름개선 효과를 가지는 천연 화장품 소재로써 응용 가능성이 있음을 시사한다. In this study, the antioxidant activities, cellular protective effects, and inhibitory effects on elastase of non-fermented and fermented extracts of Parthenocissus tricuspidata (P. tricuspidata) stem using Lactobacillus pentosus were investigated. The free radical scavenging activities (FSC₅₀) of non-fermented and fermented extracts were 42.3 and 34.5 μg/mL, respectively, in which the activity after fermentation was approximately 18.4% higher. Reactive oxygen species (ROS) scavenging activities (OSC₅₀) in Fe³⁺-EDTA/H₂O₂ system of non-fermented and fermented extracts were 2.6 and 2.5 μg/mL, respectively. The activity after fermentation was approximately 4.2% higher. In the ¹O₂-induced cellular damage of erythrocytes, the cellular protective effects (τ₅₀) of non-fermented and fermented extracts were 126.4 and 173.0 min at 50 μg/mL, respectively. The activity after fermentation was approximately 34.0% higher. The effect of fermented extract was 3.9 times higher than (+)-α-tocopherol (τ₅₀ = 43.4 min), known as a lipophilic antioxidant at 50 μg/mL. The inhibitory effect of elastase was investigated to predict the anti-wrinkle efficacy using Hs68 human fibroblasts cells. The elastase inhibitory activities (IC₅₀) of non-fermented and fermented extracts were 873.6 and 687.8 μg/mL, respectively, and the activity after fermentation was approximately 21.3% higher. These results indicated that fermented extract of P. tricuspidata stem has potentials as natural cosmetic ingredients with antioxidant and anti-wrinkle effect.

다중지능이론을 적용한 디자인 교육활동이 유아의 지능에 미치는 영향

박소연(Park So Yeon),송민정(Song Min Jung) 한국디지털디자인협의회 2012 디지털디자인학연구 Vol.12 No.4

현대사회는 탁월한 성취, 창의성 등을 두루 갖춘 창조적인 인재상을 추구한다. 이에, 창의적 인재양성을 위한 창의적 교수학습법이 화두가 되고 있다. 창의적 교수학습법의 일환으로 창의성, 자율성, 개별화 교육, 자기주도적 학습 등이 핵심 키워드로 거론되고 있으며, 이는 획일주의 교육에서 벗어나 다차원적 관점에서 바라보는 다양한 교육방법의 필요성과 중요성이 있음을 시사하고 있다. 이에 본 연구에서는 개인차 고려, 통합교과과정 적용, 학습자중심 교육을 지향하는 다중지능이론을 적용한 교수학습안을 설계하고, 학습자에게 적용 후, 학습자에게 미치는 영향에 대해 알아보고자 한다. 다중지능이론은 Howard Gardner박사가 정립한 것으로, 인간의 지능은 총 8가지 언어지능, 논리수학지능, 시각공간지능, 자연탐구지능, 개인내적지능, 대인관계지능, 음악지능, 운동지능 등으로 측정?평가할 수 있다고 한다. 그는 다중지능이론을 통해 획일화된 교육이 아닌 개인차를 고려한 학습자중심 교육의 접근법이 이루어져야 한다고 주장한다. 특히 유아의 다중지능 특성을 유아기 초기부터 발견하고 이에 적합한 개별교육이 수행된다면 유아의 흥미와 자아를 존중하는 전인교육이 수행되는 것이며 이러한 교육을 받은 유아들은 미래 사회에서 자신만의 쟁력을 갖춘 성인으로 성장할 수 있다고 지적하고 있다.1)유아교육과정의 원리요소 미루어 보아 놀이 개념의 디자인 중심 학습활동이 유아의 흥미 및 동기유발, 통합적 교과과정의 원리 측면에서 효율적인 교육적 도구가 될 수 있다. 이에 본 연구는 다중지능이론에 근거한 유아 디자인프로그램 및 교수학습안을 만들어 프로그램 적용 전?후의 결과를 비교함으로써 첫째, 유아의 디자인 교육의 필요성과 중요성에 대해 알아보고, 둘째, 다중지능이론을 적용한 디자인프로그램이 유아의 각 지능에 미치는 영향에 대해 살펴보고자 한다. In the modern society, a variety of sciences and technologies are fused to create new knowledge and value. Because of it, a creative teaching-learning method is continuously mentioned for cultivation of creative talent. Recently, creativeness, approach centered on learners and self-directed learning all of which are being emphasized in educational circles strongly indicate that each individual needs to aggressively introduce their own multiple intelligence profiles for teaching-learning process. It is the very Multiple Intelligence Theory developed by Howard Gardner that has suggested a new direction for education. He not only defined the human intelligence as standardized and single intelligence from the cognitive viewpoint but also categorized it into the total 8 fields intelligences. Howard Gardener points out that if the nature of children’s multiple intelligence can be found from early childhood and the individual education appropriate to it is to be carried out the so-called whole-rounded education respecting their interest and egos will be accomplished and those children receiving the education will grow to adults with their own competitiveness in the future. Through design education among children’s learning domains, children can find out the designs which are everywhere in our daily lives, explore the possibility of various expressions and have both convergent and divergent thoughts. Accordingly, the design education is an excellent teaching medium to make children expand the width of thought and cultivate the logic thought. Thus, for this study, a program for children’s design activities by applying Multiple Intelligence Theory will be made to figure out what influence it will have on children’s multiple intelligences.

물리․화학적 처리에 의한 요구르트 오염균의 생육 억제효과

선우찬(Chan Sunwoo),이소영(So-Young Lee),윤소영(So-Young Yoon),정지연(Ji-Yeon Jung),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),곽지희(Ji-Hee Kwak),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),정슬아(Seul-A Jung),김현지(Hyun-Jee 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.12

물리?화학적 처리에 의한 요구르트 오염균의 생육억제 효과를 알아보기 위해, 요구르트의 주요 오염균을 분리?동정하고, 열, pH, 전해수, 오존가스, microwave 처리 및 감마선을 조사하여 오염균주에 대한 사멸효과를 알아보았다. 오염된 요구르트로부터 분리한 효모의 지방산 조성 분석과 API(Analytic Profile Index) kit 분석을 실시한 결과, Hanseniaspora uvarum으로 동정되었으며 잠정적으로 Hanseniaspora uvarum Y1으로 명명하였다. H. uvarum Y1의 열 및 pH 처리에 의한 생육억제 효과를 측정한 결과, 70℃ 및 80℃에서 15분 가열처리로 균이 사멸되었으며, pH 처리 시 pH 2, 3 및 9에서 생육이 다소 억제되었으며, pH 1 및 10에서 완전히 억제되었다. 전해수 처리의 경우, clear zone이 5 mm 이상으로 H. uvarum Y1이 전해수에 높은 감수성을 가지는 것으로 나타났다. 오존가스 처리에 의한 H. uvarum Y1의 사멸효과를 측정한 결과, 102 CFU의 균은 10분, 103 CFU의 균은 20분 처리 시 모두 사멸한 것으로 나타났으며, microwave 처리의 경우, 106 CFU 가량의 균이 1분 처리 시 모두 사멸되었다. 방사선 조사의 경우, 균수를 90% 이상 감소시키는데 필요한 조사선량이 20 kGy 이상으로 H. uvarum Y1은 감마선에 저항성이 있는 균임을 알 수 있었다. 이상의 결과를 종합해볼 때 열, pH, 전해수, 오존가스, microwave 처리를 통해 요구르트 오염균주인 H. uvarum Y1의 생육을 효과적으로 억제할 수 있을 것으로 생각된다. This study was conducted to investigate the cause of microbiological contamination in yogurt and evaluate the effect of physicochemical treatment on the growth inhibition of Hanseniaspora uvarum isolated from yogurt. The yeast strain Hanseniaspora uvarum Y1 was subjected to heat and pH treatments. H. uvarum Y1 was killed at 70oC and 80oC after 15 min and survived in a wide pH range from pH 2 to 9. However, it did not survive under pH 1 and over pH 10. In a disk diffusion susceptibility test on H. uvarum Y1, a clear zone (5 mm) of growth inhibition was observed upon treatment with electrolyzed water. The effect of ozone gas on the growth of H. uvarum Y1 was evaluated by viable cell count. Initial cell numbers of 102 and 103 CFU/mL of H. uvarum Y1 were completely killed by treatment for 10 and 30 min, respectively. H. uvarum Y1 was also sterilized by microwave treatment for 1 min. When treated with gamma-irradiation, the rate of killing of H. uvarum Y1 was proportional to the irradiation dose. and complete killing occurred at a dose of 50 kGy.

물리ㆍ화학적 처리에 의한 멸균 초콜릿 우유 오염균의 생육억제 효과

최문경(Moon-Kyoung Choi),윤소영(So-Young Yoon),이소영(So-Young Lee),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),정지연(Ji-Yeon Jung),곽지희(Ji-Hee Kwak),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),이주운(Ju-W 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.8

멸균 초콜릿 우유로부터 분리한 내열성 균주에 대해 열, pH, 전해수, 오존처리, microwave 및 감마선 처리를 하여 균주의 사멸효과에 대해 알아보았다. 균주의 지방산 분석과 API kit를 통하여 균주를 동정한 결과, Bacillus lentus로 동정되었으며, 잠정적으로 Bacillus lentus M1으로 명명하였다. B. lentus M1에 110℃, 15분간 열처리하였을 경우 생육이 억제되었으며, pH 처리 시 pH 5 이하, 10 이상에서 생육이 억제된 것으로 나타났다. B. lentus M1에 대한 전해수의 항균활성을 paper disc법으로 측정한 결과, 높은 생육억제를 보였으며, 오존 처리의 경우 초기 균수가 10² CFU가량의 균을 10분 동안, 10³ CFU가량의 균을 30분 동안 처리 시 균의 생육이 억제되는 것으로 나타났다. Microwave를 1분간 처리 시 B. lentus M1이 모두 사멸한 것으로 나타났다. 감마선 조사의 경우, 1 kGy 조사 시 생균수가 1.61×10³ CFU로 초기 균수에 비해 4 log cycle 가량 균수가 감소하였으며 7 kGy에서 완전히 사멸하였다. 이상의 결과를 통해 열, pH, 전해수, 오존 처리 및 방사선 처리 방법이 멸균 초콜릿 우유의 생존 오염균인 B. lentus M1을 효과적으로 사멸시킬 수 있을 것으로 사료된다. This study was conducted to investigate the cause of microbiological contaminants in aseptic chocolate milk and evaluate the effect of a physicochemical treatment on the growth inhibition of isolated bacterial strains. The bacterium isolated from aseptic chocolate milk was identified as Bacillus lentus and was named B. lentus M1. In the heat and pH treatment, the growth of B. lentus was inhibited at 110℃ for >15 min and at pH’s <5 and >10. An electrolyzed water treatment against B. lentus M1, revealed 5 ㎜ growth past the inhibition zone. The effect of ozone gas on B. lentus M1 growth was evaluated using viable cell counts. When the initial number of B. lentus M1 was 10² and 10³ CFU, the bacteria were completely suppressed by ozone gas treatment for 10 and 30 min, respectively. In a microwave treatment, B. lentus M1 was sterilized following microwave treatment for 1 min. As the result of γ-irradiation against B. lentus M1, numbers decreased as the γ-irradiation dosage increased. These results show the growth inhibition effects against contaminants in aseptic chocolate milk using physicochemical treatments.

CELP 보코더에서 델타 피치 검색 방법 개선에 대한 연구

민소연,배명진 한국음성과학회 2004 음성과학 Vol.11 No.3

In this paper, we propose the computational complexity reduction methods of delta pitch search that is used in G.723.1 vocoder. In order to decrease the computational complexity in delta pitch search the characteristic of proposed algorithms is as the following. First, scheme to reduce the computational complexity in delta pitch search uses NAMDF. Developed the second scheme is the skipping technique of lags in pitch searching by using the threshold value. By doing so, we can reduce the computational amount of pitch searching more than 64% with negligible quality degradation.

임부의 연령에 따른 보완대체요법에 관한 인식 태도 경험 비교 연구

김연진,김지연,노경민,신혜리,유예지,이지민,장희조,정아인,최소정,강숙정,이향연,민혜영 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: This study aims to test if the age of pregnant women affects the perception, experience, and attitude towards complementary alternative therapy (CAT), by investigating the perception, experience, and attitude of pregnant women under the age of 35, and above 35 (Advanced Maternal Age). The study will 1) find the general characteristics of pregnant women under and over the age of 35 2) compare the perception, behavior, and experience of pregnant women under and over the age of 35, and 3) find the comparison of the perception, behavior, and experience according to the general characteristics of pregnant women under and over the age of 35. Method: The subjects of this study are composed of 44 women under the age of 35 and 44 women above the age of 35, totaling 88 pregnant women visiting midwiferies, obstetrics and gynecology, and baby fairs in Seoul and Gyeong-Gi area. Data for this study was collected from November 28, 2015 to January 6, 2016 for 40 days. The collected data analyzed mean, standard deviation, t test, ANOVA, and chi-square by using SPSS 23.0. Result Results show that there is a significant difference in the perception, attitude, and experience of the two-sample groups in the use of CAT. The question of “what is most necessary when applying CAT in a hospital clinical environment” for the perception criteria came out with the results of t=9.980, p<.05. For the attitude, results showed that the answers of pregnant women under the age of 35 displayed a more positive attitude towards CAT. (t=2.421, p<.05) Lastly, in the experience section, pregnant women under the age of 35 showed more CAT use than the other sample group. Conclusion Results have shown that there is a difference in the perception, behavior, and experience between the two sample groups. Therefore further research on the efficacy and the adverse effects of CAT needs to be done.

워크플로우(WorkFlow) 모델링에 관한 연구

김소연,성국경,이승민 공주영상정보대학 1998 논문집 Vol.5 No.-

본 연구에서는 기존 워크플로우 관리 모형이 구문적이고, 순서적인 표현 방법의 문제점을 해결하고, 비지니스 업무의 특성상 병행적, 분산적, 실시간적, 비동기적으로 발생되는 활동과 시간 인원, 도구, 비용 등 자원을 효과적으로 모형화하는 방법을 제시하기 위한 도구로써 패트리넬을 제시한다. 본 연구 결과를 통해 비지니스 업무의 특성인 프로세스의 불확실성(uncertainty) 활동간의 병행성(parallelism)과 비동기성(asynchronization), 환경의 변화성(evolution) 활동의 추상성(abstraction) 그리고 프로세스간의 협동성(coorperation) 및 분산성(distribution)들을 시각적인 모형으로 가시화(visualization)시키고, 공식화(formalism), 자동화(automation), 정량화(qualification), 수행가능(executability)할 수 있는 분산환경을 위한 워크플로우 관리 모형을 효과적으로 모형화하는 기초 연구로서 본 연구가 활용될 수 있다. 본 연구 결과를 이용해 GUI 환경의 범용 패트리넬 분석기를 개발하여 분석하는 일을 향후 연구 과제로 남겨두었다.

마쇄처리 고추의 냉동저장중 이화학적 성분의 변화

설민숙,황성연,이현자,박소희,김종군 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.2

To investigate physico-chemical changes of mashed red repper and red pepper powder during frozen storage, we analyzed the quality of samples after mashing, mashing and blanching, mashing and addition of vitamin C, mashing and addition of NaCl. The most important quality factor of red pepper is red color. α value of red pepper powder of Pochungchun was 31.51. These results showed that those processing methods were effective to preserve red color. Capsanthiin contents of mashed red pepper treated with vitamii C were decreased 1.8% after 180 days of frozen storage. Thii decrease was the Least, showing that addition of vitamin C helped retaining capsanthiin contents. Capsaicin and dihydrocapsaicin contents in Pochungchun powder were decreased 11.9 and 18.3% during frozen storage respectively. These results showed that capsaicin was more stable than dhycrocapsaicin during frozen storage. Generally capsaicin and dhycrocapsaicinin the Pochungchun red pepper frozen-stored after mashing were less decreased than red pepper powder, and addition of vitamin C to the mashed red pepper was the most effective to retain capsaicin and dihydrocapsaicin than others. The content of vitamin C in the red pepper frozen-stored after mashing and blanching was 12.0 mg/100g, compared with 44.0mg/100g in the sample stored after only mashing. This suggested that blanching process destroyed vitamin C in the mashed red pepper. Addition of salt in the mashed red pepper showed the same inclination. But, addition of vitamin C to the mashed red pepper was decreased 32.9%, compared with 69.0% in the mashed red repper during frozen storage. Theser results indicated that addition of vitamin C could retain vitamin C in the mashed red repper.

관절염으로 발현한 재발성 다발성 연골염 1례

엄민식,박원우,서승철,이진호,조주연,최혜영,이찬희,임소덕 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Relapsing polychondritis is an uncommon multisystemic disease which is characterized by recurrent episodes of inflammation and progressive destruction of cartilaginous tissue. The cause of relapsing polychondritis is unclear and suspected to be autoimmune pathophysiology. It involves cartilage of nose, vertebra, trachea, peripheral joint as well as structures of eye, heart, blood vessels or inner ear. Auricular chondritis is present in almost all patients, and multiple arthritis, nasal chondritis, saddle nose, eye involvement, laryngotracheal involvement and skin manifestations are also present. We report a case of relapsing polychondritis presented a arthritis with review of literatures.