Streptozotocin-당뇨병 백서에서 당뇨병 이환기간에 따른 대동맥의 이완능 저하 및 Oxygen-Free Radical에 의한 이완능의 손상정도

김정국,김신우,김수동,서예경,하승우,김보완,백운이,권삼,강승완 경북대학교 병원 1997 경북대학교병원의학연구소논문집 Vol.1 No.1

목적: 당뇨병의 이환기간이 혈관의 내피세포 의존성 및 비의존성 이완능의 저하에 미치는 영향과 이환기간에 따라 oxygen-free radical(OFR)에 의한 동맥이완능의 손상정도에 차이가 있는지를 알아보고자 하였다. 방법: Streptozotocin(STZ)으로 당뇨병이 유도된 3 주 및 10주째의 백서군과 연령이 비슷한 정상 대조백서군의 적출 대동맥환을 norepinephrine으로 수축시킨 상태에서 내피세포 의존성 이완제인 acetylcholine(ACh) 및 비의존성 이완제인 nitroglycerine(NTG)에 대한 이완능을 즉정하여 비교 관찬하였다. 또한 xanthine과 xanthine oxidase의 혼합으로 생성된 H₂O₂에 대동맥환을 노출시킨뒤 역시 ACh과 NTG에 대한 이완능의 손상정도를 비교하였다. 결과: 3주및 10주째 당뇨병 백서의 대동맥환은 모두 대조군 백서의 대동맥환에 비해 ACh에 대한 이완능이 유의하게 감소하였으며 10주째 당뇨병 백서군에서의 ACh에대한 이완능의 저하는 3 주째 당뇨병 백서군에 비해 유의하게 심하였다.H₂O₂에 노출시킨 뒤 ACh에대한 이완능은 3주 및 10주 째 당뇨병 백서군에서 모두 대조군에 비해 유의하게 심한 손상을 보였으나 3주와 10주째 당뇨병 백서군 사이의 손상정도의 차이가 통계학적으로 유의하지는 않았다. 한편 3주 및 10주째 당뇨병 백서군 모두 NTH에 대한 대동맥환의 이완능은 대조 백서군과 별다른 차이가 없었으며 또 H₂O₂에 노출시킨 뒤에도 NTG에 대한 이완능의 손상은 관찰되지 않았다. 결론: STZ-당뇨병백서에서 대동맥의 이완능은 당뇨병에 이환된지 3 주째에 이미 ACh에 대한 내피세포 의존성 이완능이 선택적으로 저하되며 당뇨병의 이환기간이 길수록 이완능의 저하가 심하였고, OFR에 의한 동맥 이완능의 손상도 내피세포 의존성 이완능에만 선택적으로 나타나나 이환기간에 따라 OFR에 의한 이완능 손상정도에는 뚜렷한 차이가 없음을 시사하고 있다. Objectives: Diabetes mellitus produces histological evidence of endothelial as well as changes in vascular function in experimental animal. Oxygen free radicals interfere with of destroy endothelium-dependent relaxation in normal and diabetic blood vessels. The present study was investigated whether the duration of diabetic state can affect the severity of impairment in endothelium-dependent and/or independent relaxation and the sensitivity to oxygen free fadical (OFR)-induced damage in diabetic rat vasculature. Methods: The relaxative responses of aortic ring precontracted with norepinephrine in vitro to cumulative concentrations of acetylcholine (ACh) and nitroglycerine (NTG) were conducted in 3- and 10- week streptozotocin (STZ)-diabetic rats and agematched controls. After exposure to H₂O₂generated by mixing xanthine and xanthine oxidase, the degree of impairment in relaxative responses to ACh and NTG were also compared between 3- and 10-week diabetic rats. Results: The relaxative responses of aortic rings produced by cumulative concentrations of ACh were significantly lower in both 3- and 10-week diabetic rats than in the age-matched controls. The impairments of relaxative responses to ACh in 10-week diabetic rats were significantly greater than those in 3-week ones. After H₂O₂ exposure, the impairments of relaxative responses to ACh in 3- and 10-week diabetic aortas was also significantly greater than those in the control in the control ones. However, the severity of H₂O₂-induced damage in 10-week diabetic aortas was not significantly different than that in 3-week ones. In contrast, aortic rings of 3- and 10-week diabetic rats could still be fully relaxed by NTG, even after exposure to H₂O₂. Conclusion: These findings suggest that in SRZ-diabetic rat aortas selective impairment of endothelium-dependent relaxation to Ach can be found early even over a 3-week period after the induction of diabetes and the impairment may be greater in those with the longer duration of the diabetic state and that the impairment of the vascular relaxation by OFR is also selective to endothelium-dependent response but the sensitivity of OFR-induced damage may not be related to the duration of the diabetic state.

Effect of Autoclave and Gamma-Irradiation Treatmentson Allergenicity of Pork Ham

Seo-Jin KIM,Koth-Bong-Woo-Ri KIM,So-Young LEE,Eu-Jin SONG,So-Young YOON,So-Jeong LEE,Chung-Jo LEE,Jin-Gyu PARK,Ju-Woon LEE,Myung-Woo BYUN,Kyu-Earn KIM,Dong-Hyun AHN 한국생물공학회 2009 한국생물공학회 학술대회 Vol.2009 No.4

Pancreatic serous cystic neoplasms accompanying other pancreatic tumors

Kim, So-Woon,Song, In Hye,An, Soyeon,Kim, So Yeon,Kim, Hyoung Jung,Song, Ki-Byung,Hwang, Dae Wook,Lee, Sang Soo,Byun, Jae Ho,Seo, Dong-Wan,Kim, Song Cheol,Yu, Eunsil,Hong, Seung-Mo Elsevier 2017 Human pathology Vol.60 No.-

<P>Serous cystic neoplasms (SCNs) are benign cystic neoplasms that predominantly occur in the tail of the pancreas in elderly women. It is well known that patients with von Hippel - Lindau syndrome can develop SCNs and neuroendocrine tumors in the pancreas. However, our understanding on SCNs accompanying other pancreatic tumors (SCNAOPTs) is limited. We compared the clinicopathological features of 15 surgically resected SCNAOPTs with 259 conventional SCNs. The prevalence of SCNAOPT was 5%. The SCNAOPTs were significantly smaller than conventional solitary SCNs, and they were more commonly observed in the head of the pancreas, whereas conventional solitary SCNs were more frequently noted in the body and tail. However, no differences were found in terms of sex, patient age, or the gross patterns of the SCNs. Accompanying neoplasms included 7 intraductal papillary mucinous neoplasms, 1 colloid carcinoma arising from intraductal papillary mucinous neoplasm, 6 neuroendocrine tumors, and 1 solid pseudo papillary neoplasm. Four neuroendocrine tumors associated with von Hippel Lindau syndrome occurred as multiples, whereas 2 neuroendocrine tumors without von Hippel Lindau syndrome were solitary. In summary, SCNAOPTs comprise 5% of all SCNs and tend to be smaller and located in the head of the pancreas. Common accompanying tumors include intraductal papillary mucinous neoplasms, neuroendocrine tumors, and other neoplasms such as colloid carcinoma and solid pseudopapillary neoplasm. (C) 2016 Elsevier Inc. All rights reserved.</P>

Increase of Resveratrol Content and Gene Expression Level According to Days after Seedling in Transgenic Rice

Woon-Chul Shin,So-Hyeon Baek,Chun-Sun Seo,Ki-Young Kim,Jong-Cheol Ko,Young-Jun Mo,Jeong-Kwon Nam,Bo-Kyeong Kim,Jae-Kwon Ko 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Resveratrol (3,5,4'-trihydroxystilbene) is a naturally occurring antioxidant found in grapes, grape products such as wine, and some other botanical sources, like peanuts. In grapes, resveratrol occurs both as free resveratrol and piceid, which is 3-β-mono-D-glucoside of resveratrol. In recent years, research on resveratrol has discovered several beneficial biological effects of this compound to human health. These include anticancer activity, cardioprotection, antioxidant activity, inhibition of platelet aggregation, and antiinflammatory activity. So, we conducted this study to develop transgenic rice plants an enhanced phytochemicals associated with reduced cancer risk by overexpression of a peanut RS gene and selected 2 lines, 'Iksan 515' and 'Iksan 526', according to agronomic traits of rice plant and resveratrol contents in rice grain. Resveratrol content of Iksan 515 was increased by 2.3 times until 5 days after seedling and decreased at 7 days after seedling. Expression level of RS gene according to seedling days by Real-time PCR increased until 5 days, but decreased by half at 7 days. Now, we are planning to analyze medicinal function of these high resveratrol rices and development of cultivation technology to increase resveratrol content. Therefore, transgenic rices producing high resveratrol can be used to develop new rices with a cancer chemopreventive role in human medicine.

Case Report : Uterine Lipoleiomyoma in Peri or Postmenopausal Women

( So Ra Oh ),( Yeon Jean Cho ),( Myoungseok Han ),( Jong Woon Bae ),( Jung Woo Park ),( Seo Hee Rha ) 대한폐경학회 2015 대한폐경학회지 Vol.21 No.3

Lipoleiomyoma is an uncommon neoplasm of the uterus, composed of smooth muscles intermixed with mature adipocytes. These tumors are considered a benign variant of uterine leiomyomas. Herein, we report six cases of lipoleiomyoma experienced in our institution from January 2005 to March 2015. The patients ranged in age from 45 to 70 years; the etiology may be related to estrogen deficiency occurring after menopausal transition. Except for one lipoleiomyoma in the broad ligament, all others were found in the uterine corpus. The presenting symptoms were nonspecific, and most cases were incidentally diagnosed during surgery for other reasons. We performed preoperative imaging studies, including abdominal and pelvic computed tomography and magnetic resonance imaging. Preoperatively, four patients were diagnosed as having a pelvic mass and one patient was diagnosed as having a right ovarian mature teratoma. In one case, we found a gynecologic malignancy (cervical cancer 1A1). Histologically, there was no gross or microscopic contiguity between the lipoleiomyoma and the malignancy. Lipoleiomyomas seem to have a benign clinical course. In our study, there were no recurrences of or deaths attributed to the lipoleiomyomas during a mean follow-up period of 16.17 ± 23.80 months. (J Menopausal Med 2015;21:165-170)

Menopause is a risk factor for Bartholin gland cysts and abscesses

( So-jung Kweon ),( Jin-sung Yuk ),( Seo Yoo Hong ),( Ahm Kim ),( Dae Woon Kim ) 대한산부인과학회 2019 대한산부인과학회 학술대회 Vol.105 No.-

Objective: To evaluate the effects of menopause on benign Bartholin gland disease and the incidence of benign Bartholin gland disease using Korean national insurance data. Methods: In this case-control study, a total of 3,729 women with Bartholin gland disease were selected, and 14,916 women were selected as the control group by 1:4 propensity score matching for age per 5 years, socioeconomic status (SES), and data year from the Health Insurance Review & Assessment Service National Patient Sample(HIRANPS) 2010-2016 data. We performed logistic regression analysis with Bartholin gland disease as a dependent variable and menopause as an independent variable. Results: The incidence of all Bartholin gland disease was 7.2±0.1 per 10,000 person-years in all age groups and 11.4±0.2 per 10,000 person-years in the 15-50 age group. The incidence of Bartholin gland disease peaked in women in their late 40s and sharply decreased thereafter. In the logistic regression analysis, which was adjusted for age per 5 years, SES, and data year, menopause increased Bartholin gland disease [odds ratio (OR) 2.41, 95% confidence interval (CI) 2.01-2.88]. This pattern was similar to that for Bartholin gland cysts [OR 2.51, 95% CI 1.96-3.22] and Bartholin gland abscesses [OR 1.84, 95% CI 1.49-2.28]. However, menopausal hormone thearpy showed a slight tendency toward reducing Bartholin gland disease, though the trend was not statistically significant [OR 0.51, 95% CI 0.23-1.13]. Conclusion: In conclusion, the incidence of Bartholin gland disease peaks in women in their late 40s and sharply decreases thereafter. However, menopause increases the incidence of Bartholin gland disease. This seem to be a problem of threshold values due to estrogen levels.

형광 처리한 자궁경부암 세포의 광학 특성 분석을 위한 산란 검출 시스템 설계

김소영(So-Yeong Kim),장원진(Won-Jin Jang),서해송(Hea-Song Seo),이지은(Ji-Eun Lee),김상희(Sang-hee Kim),최세운(Se-woon Choe) 한국정보통신학회 2021 한국정보통신학회 여성 ICT 학술대회 논문집 Vol.2021 No.8

유동 세포 분석법은 산란하는 물리적 현상을 이용한 세포에 대한 다양한 광학적 특성을 획득하고 분석하는 장비이다. 하지만 유동 세포 분석법은 고가이며 적절한 공간과 전문가가 필요하다는 단점을 가지고 있다. 본 연구에서는 LED, 포토다이오드, 아두이노 메가 ADK, 3D 프린터를 이용해 저가 및 소형화 유동 세포 분석법을 설계했다. Calcein AM과 같은 형광물질을 이용하여 형광 처리한 자궁경부암 세포를 투과시킨 결과, 빛의 이동 방향을 따라 수집된 광신호에서 형광 검출을 확인할 수 있었다. A flow cytometer using the physical phenomenon of spawning is a device that obtains and analyzes various optical properties of cells. However, flow cytometer has the disadvantage of requiring expensive, appropriate space and experts. In this study, LED, photodiode, ADK and 3D printers were used to design cheap miniaturized flow cytometer. As a result of evaluating the performance of the system by fluorescing cervical cancer cells with Calcein AM, it was possible to detect fluorescence from the light collected in front of the light travel direction.

Analysis of low molecular weight plasma proteins using ultrafiltration and large gel two-dimensional electrophoresis

Jung, Woon-Won,Phark, Sohee,Oh, Sangnam,Khim, Jin-Young,Lee, Juneyoung,Nam, Myung Hee,Seo, Jong Bok,Park, So-Young,Jo, Eunkyung,Choi, Seonyoung,Zheng, Zhi,Lee, Ji Young,Lee, Min,Lee, Eunil,Sul, Dongge WILEY-VCH Verlag 2009 Proteomics Vol.9 No.7

<P>In this study, various solvent systems were applied to obtain a high and consistent recovery rate of low molecular weight plasma proteins (LMPP) from human plasma. A buffer system containing 7 M urea, 2 M thiourea, 25 mM NH<SUB>4</SUB>HCO<SUB>3</SUB> + 20% ACN (pH 8.2) produced the highest recovery rate of LMPP. To validate the recovery of cut off membrane (COM) obtained using the urea buffer system, 27 different 30 kDa COMs were used to prepare the LMPP sample which were then subjected to 1-D SDS-PAGE. Statistical analysis showed that the buffer system with COM produced a consistent the recovery of LMPP. In addition, 2-DE analysis was also conducted to determine the relative intensity of each protein spot. When molecular weight ranges over 30 kDa and under 30 kDa were evaluated, 953 and 587 protein spots were observed in the gels, respectively, resulting in a total of 1540 protein spots being resolved. Identification of the major proteins were then performed using a nano-LC/MS system comprised of an HPLC system and an ESI-quadrupole IT MS equipped with a nano-ESI source.</P>

신장주위 거대혈종을 보였던 신중후성 출혈열 1예

박운형,김영아,정재한,최소래,강태수,서정건,박흥수,하성규 대한신장학회 2000 대한신장학회잡지 Vol.19 No.6

고령장애인의 고용유지에 영향을 미치는 요인에 관한 연구

서지혜 ( Seo Ji-hye ),나운환 ( Na Woon-hwan ),오소윤 ( Oh So-yun ) 대구대학교 특수교육재활과학연구소 2016 특수교육재활과학연구 Vol.55 No.4

본 연구는 고령장애인의 고용유지 영향요인을 규명하여 고령장애인의 특성에 맞는 고용 대안을 제시하는데 목적이 있다. 연구목적을 달성하기 위하여 한국장애인고용공단에서 실시한 2014년 제7차 장애인고용패널조사 원자료(raw data)를 활용하여 분석을 실시하였으며, 연구의 결과는 다음과 같다. 첫째, 고령장애인 취업자 대부분은 남성이고, 고용서비스와 직업훈련을 받은 경험이 없으며, 근로자수가 50명 미만이며 노조가 없는 소규모 사업체에서 종사중인 것으로 나타났다. 둘째, 고령장애인은 평균임금이 높을수록, 신체외부장애를 가진 경우, 고용서비스 이용 경험이 없는 경우에 고용유지기간이 긴 것으로 나타났다. 셋째, 고령장애인의 고용유지에 영향을 미치는 변수는 경제적 요인의 평균임금, 장애 및 건강 요인의 장애유형, 인적자본요인의 고용서비스 경험이다. 넷째, 고령장애인과 비고령장애인 두 집단 모두 고용서비스를 받은 경험이 없는 경우, 좋은 일자리에 종사하는 경우에 고용유지기간이 길었으며, 고령장애인은 인구학적 요인과 거주형태가 자가인 경우, 장애유형이 신체외부장애인 경우에 고용유지기간이 길게 나타났지만, 비고령장애인은 정규근로시간에 가까울수록 고용유지기간이 길게 나타나는 차이가 있다. 이러한 연구결과를 바탕으로 고령장애인의 고용유지를 위한 제언을 제시하였다. The purpose of this study is to suggest the policy alternatives for the characteristics of elderly people with disabilities by investigating analysis the factors that influence the employment maintenance for elderly people with disabilities. To achieve this study purpose, using the data set of seventh-year Panel Survey of Employment for the Disabled. The targets of analysis are wage earners who are over 50 years of age and the others, and followings are the result; First, most elderly people with disabilities are employed male, haven`t experience in vocational training and employment services. They work in small companies less then 50 workers and without a trade union. Second, elderly people with disabilities has a longer period of time maintaining employment when they have higher average wages, have a physical disability and haven`t employment service experience. Third, variables affecting job maintaining for elderly people with disabilities is the average wage of the economic factors, the type of disability of disability and health factors, the experienced employment services of human factors. Fourth, elderly people with disabilities and people with disabilities has a longer period of time maintaining employment when they didn`t experience employment services, working in good jobs(higher wages, full-time, presence of unions). Elderly people with disabilities has a longer period of time maintaining employment when they are male, have a spouse, large number of families, home ownership, the physical disabilities. But non-elderly people with disabilities has a larger period of time maintaining employment when they work 40 hours per week. Thus, based on the analysis results, to present some suggestions and limitations for maintaining employment of the elderly people with disabilities, is as follows: First, the development of a variety of employment services for the elderly people with disabilities, in this process, there is a need for the development of services and policies for women elderly people with disabilities. Second, there is need for the development of a variety of support services and policies for improvement of the qualitative level of employ services and the people with internal disabilities`s job maintaining. Third, people with disabilities won a good job, so that it can be maintained, it is necessary to provide appropriate policies and life-long service. Fourth, This study have a limit to the display in consideration of the employment status of the entire country, because it compared the population of elderly people with disabilities only in the population of the non-elderly persons with disabilities. Therefore, in the subsequent study, there is a need for comparative analysis study of the employment status of the entire country.