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Comparison of Proteome Components of Helicobacter pylori Before and After Mouse Passage
Lee, Kyoung-Ja,Kim, Bok-Ran,Cho, Young-A,Song, Yun-Gyu,Song, Jae-Young,Lee, Kon-Ho,Kang, Hyung-Lyun,Baik, Seung-Chul,Cho, Myung-Je,Rhee, Kwang-Ho,Seo, Ji-Hyun,Youn, Hee-Shang,Lee, Woo-Kon 대한미생물학회 2011 Journal of Bacteriology and Virology Vol.41 No.4
The mouse model is alleged to be a useful tool for understanding of pathophysiological roles of Helicobacter pylori in the development of gastric disorders. However, it has been observed that H. pylori strains significantly differed in their fitness in mice and even mouse strains differed in their susceptibilities to a H. pylori strain. Bacterial components of H. pylori which could affect on its fitness in mice have to be elucidated for the establishment of the mouse model for H. pylori infections. In the comparison of colonization ability between two H. pylori Korean isolates, 51 (isolated from a patient with duodenal ulcer) and 52 (isolated from a patient with gastric cancer), 52 could colonize better than 51 on the gastric mucosa of mouse. Proteome components of H. pylori 52, as a good colonizer and H. pylori 51, as a poor one were quantitatively compared each other. Five bacterial proteins including catalase, urease subunit alpha/beta, enolase and ferritin, were up-regulated in 52. In addition, the respective proteome components of the two strains were also compared with their mouse-passaged homologous strains. Seven and five proteins, which included catalase, flagellin A/B in common, were up-regulated in mouse-adapted 51 and 52, respectively. Among the fourteen identified proteins, urease subunit alpha/beta, flagellin A/B, catalase, ferritin, superoxide dismutase and neutrophil-activation protein have been previously known to be necessary to gastric colonization of H. pylori in animal models. The other up-regulated proteins including enolase, elongation factor Tu and fructose-bisphosphate aldolase have been reported to be associated with acid tolerance of H. pylori. These data provide confirmatory evidence for the importance of those proteins in the development of H. pylori-associated gastric disorders.
( Myung Woo Choi ),( Jong Hwa Lee ),( Sung Sik Oh ),( Mi Rim Choi ),( Hyun Ju Yang ),( Moon Sik Park ),( Jae Un Lee ),( Shang Hoon Han ),( Young Jae Lee ),( Yong Keun Cho ),( Ji Woong Kim ),( Ji Woong 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Introduction: There are various tumorous lesions in pancreas, but it is diffi cult to differenciate these lesions because of the anatomical and clinical characteristics. Through endoscopic-ultrasound(EUS)-guided biopsy, the effort for increasing diagnostic rate is still ongoing. However, Sometimes it is diffi cult to get enough tissue through EUS and to get accurate diagnostic imaging of tumorous lesion. In that case, the defi nitive diagnosis is only obtained after surgical intervention. Authors report one case, which was diagnosed as the pancreatic cancer before the operation and had a pancreaticoduodenectomy, but the lesion was proved to be tumor forming chronic pancreatitis on histological examination of a frozen-section. Case: A 65-years-old male patient presented with indigestion for about 1week and he was found to have a tumorous lesion in pancreas by abdominal-ultrasonography at local. He was a chronic drinker who consumed 100g of alcohol everyday. On initial laboratory evaluation, PT-1. 02, AST/ALT-22/12U/L, ALP-342IU/L, T-bilirubin-0. 2mg/ dL, Amylase-74U/L, lipase-160U/L, CA19-9-9. 13U/mL, CEA-6. 1ng/mL. In Contrast-enhanced abdominal-CT, 6. 4x5. 0x4. 0cm mass was found in the pancreas head, which was low density and little more enhanced than normal pancreas and the boundary was relatively clear. EUS-guided Procore 25G-needle biopsy and Cytology was done, but only normal pancreas tissues were found. However there was FDG uptake (SUVmax- 3. 79) in the head of pancreas on PET-CT. So we diagnosed this case as pancreas- cancer in T3N1 and he underwent pancreaticoduodenectomy. After the operation, fi nal histological diagnosis was mass forming chronic pancreatitis. Conclusion: Chronic pancreatitis can present with a fi brotic mass. If there is a risk factor like chronic alcohol intake in medical history taking and CA 19-9 is normal, it could be the chronic pancreatitis which has a possibility of forming mass rather than pancreatic cancer. In conclusion, it needs more effort of imaging examination and tissue confi rmation for accurate diagnosis.
Comparison of Proteome Components of Helicobacter pylori Before and After Mouse Passage
Kyoung-Ja Lee,Bok-Ran Kim,Young-A Cho,Yun-Gyu Song,Jae-Young Song,Kon Ho Lee,Hyung-Lyun Kang,Seung-Chul Baik,Myung-Je Cho,Kwang-Ho Rhee,Ji-Hyun Seo,Hee-Shang Youn,Woo-Kon Lee 대한미생물학회 2011 Journal of Bacteriology and Virology Vol.41 No.4
( Jong Hwa Lee ),( Myung Woo Choi ),( Sung Sik Oh ),( Mi Rim Choi ),( Hyun Ju Yang ),( Moon Sik Park ),( Jae Un Lee ),( Shang Hoon Han ),( Young Jae Lee ),( Yong Keun Cho ),( Ji Woong Cho ),( So Hee Y 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Introduction: Infi ammatory fi broid polyp is a rare benign tumor of the gastrointestinal tract. The most common site of the tumor is the gastric antrum, followed by ileum. It originates from the submucosa. It is composed of fi brous tissue, blood vessels and numerous infi ammatory cells such as eosinophils. In the cases of infi ammatory fi broid polyp in the ileum, most of polyps are solitary lesions and they usually present as intestinal obstruction or intussusception. Case: A 42 year old male presented epigastric pain. He had peptic ulcer 2 years ago. Under endoscopy, he had peptic ulcer with partial gastric outlet obstruction again. But, CT (computerized tomography)scan showed ileocolic intussusception secondary to ileal mass. Laparoscopic hemicolectomy was performed. The ileal mass was an infi ammatory fi broid polyp. Conclusion: It was diffi cult to diagnose this intusscusception secondary to infi ammatory fi broid polyp in the ileum because of partial gastric outlet obstruction. Careful investigation is important to diagnose this disease exactly.
10 년간 경상의대 의학과 학생의 A 형 간염 항체 양성률 변화와 경상의대 소아과 근무 의료인의 항체 양성률
임재영,고경혁,조명제,이시은,조중현,윤희상,백승철,이우곤,이광호,우향옥,조윤경,박찬후,정양숙,이수진 대한소화기학회 1999 대한소화기학회지 Vol.33 No.4
Background/Aims: Korean population has been suggested to be minimally exposed to hepatitis A virus (HAV) for recent 20 years. Therefore, hepatitis A epidemics may occur among medical students and medical personnels who care the asymptomatic patients with hepatitis A. We investigated the changes in anti-HAV IgG positive rate among the medical students for 10 years and the curren anti-HAV IgG positive rate among the medical personnels in Chinju. Methods: Serum anti-HAV IgG was measured using the commercially available HAVAB radioimmunoassay kit. The sera had been collected from 780 sophomore medical students during 1988-1997 and from 95 pediatric medical personnels at the Gyeongsang National University Hospital in December, 1997. Results: The anti-HAV IgG positive rate of the sophomore medical students was 87.5% in 1988, 86.7% in 1989 83.8% in 1990, 75% in 1991, 85% in 1992, 70% in 1993, 56.3% in 1994, 58.8% in 1995, 53.8% in 1996 and 38.8% in 1997. The anti-HAV IgG positive rate among the pediatric medical personnel (mean age ±S.D.; 26.4 ±3.1) was 65.3%. Conclusions: These suggest that the population susceptible to HAV exists among medical students and pediatric medical personnels. Therefore, we should se up the preventive modalities against hepatitis A virus infection among these high risk groups.
Lee, Woo-Kon,Kim, Mi-Hye,Song, Jae-Young,Kim, Sam-Churl,Park, Jeong-Uck,Baik, Seung-Chul,Kang, Hyung-Lyun,Park, Seong-Gyu,Hwang, Hyang-Ran,Bae, Dong-Won,Youn, Hee-Shang,Ko, Gyung-Hyuck,Cho, Myung-Je The Korean Society for Microbiology 2004 Journal of Bacteriology and Virology Vol.34 No.4
Low-abundance cellular proteins normally invisible on the standard two-dimensional SDS-polyacrylamide gel electrophoresis (2-DE SDS-PAGE) map must be enriched appropriately in order to be visualized and identified in cells or tissues. We applied proteins of H. pylori strain 26695 to a immobilized heparin-affinity resin, which has an affinity for nucleic acid-binding proteins, protein biosynthesis factors, and growth factors. The whole cell extract of H. pylori strain 26695 was fractionated by the heparin-agarose chromatography, and was analyzed by 2-DE. The 2-DE SDS-PAGE displayed spots after silver staining, which were identified by matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS). Among the ca. 150 spots that were processed, 79 proteins representing 57 genes were identified. Eleven proteins were determined to be nucleic acid-associated. Eighteen proteins were newly identified in this study, including DNA topoisomerase I. These results may provide guidance for enriching low abundance proteins of H. pylori and contribute to the construction of a master protein map of H. pylori.