배추의 형질전환용 선발항생제로서 Paromomycin의 이용

조미애,민성란,고석민,유장렬,이준행,최필선,Cho, Mi-Ae,Min, Sung-Ran,Ko, Suck-Min,Liu, Jang-Ryol,Lee, Jun-Haeng,Choi, Pil-Son 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.4

정상' 배추와 '서울' 배추의 배축절편을 선발마커로서 hygromycin 저항성유전자를 갖고 있는 pCAMBIA1301와 paromomycin저항성유전자를 갖고 있는 pPTN290으로 각각 형질 전환된 LBA4404 또는 EHA101균주와 공동배양한 후 선발배지에서 배양하면서 형질전환체를 선발하였다. 형질전환빈도는 사용된 항생제와 품종에 따라서 현저하게 차이가 있었으며, 특히 paromomycin은 hygromycin보다 효과적이었고 정상 배추는 서울배추보다 양호하였다. 가장 높은 형질전환빈도는 (0.70%) 100mg/L paromomycin이 첨가된 선발배지에서 정상배추의배축을 배양할 경우 얻어졌다. GUS양성반응으로 확인 한 결과 정상배추에서 9개체와 서울배추에서 3개체를 각각 얻었으며, 온실에서 생장한 후 $T_1$종자를 수확하였다. $T_1$ 종자를 다시 발아시켜 유식물체를 얻은 후 GUS양성반응을 확인함으로서 외래유전자가 안정적으로 발현하고 있음을 확인하였다. Hypocotyl explants of Chinese cabbage (us. 'Jeong Sang' and 'Seoul') produced adventitious shoots on Murashige and Skoog (MS) basal medium supplemented with 4mg/L $AgNO_3$, 5 mg/L acetosyringone, 4 mg/L 6-benzyladenine and 3mg/L alpha-naphthaleneacetic acid (SI) after cocoultivation with strains of Agrobacterium tumefaciens (LBA4404) harboring the pCAMBIA1301 and the $_PPTN290$ containing hygromycin-resistance gene and paromomycin-resistance gene as a selectable marker genes, respectively. There was a significant difference in the frequency of transgenic plants depending on antibiotics and cultivars used. Paromomycin was better than hygromycin, and cultivar 'Jeong-sang' was higher than 'c.v. Seoul' in the frequency of transgenic plants. In particular, the highest frequency (0.70%) of transgenic plants was obtained from selection medium (SI) containing 100mg/L paromomycin in c.v., 'Jeong-sang' GUS positive response were obtained 9 plants and 3 plants from the cultivars, 'Jeong-sang' and 'Seoul', respectively. They were grown to maturity in a greenhouse and normally produced $T_1$ seeds. GUS histochemical assay for progeny $(T_1)$ revealed that the transgenes were expressed in the plant genome.

<SUP>18</SUP>F-FDG PET-CT에서 진단된 갑상선 우연종의 악성종양 발견율 및 특징

이강영,고건<SUP>1<.SUP>,김선국<SUP>1<.SUP>,고진철<SUP>1<.SUP>,김행수,최상용,박신희,박용휘<SUP>2<.SUP>,Kang Young Rhee,Gun Koh,<SUP>1<.SUP>,Sun Kuk Kim,<SUP>1<.SUP>,Jin Chul Koh,<SUP>1<.SUP>,Haeng Soo Kim,Sang Yong Choi,Shin 대한갑상선-내분비외과학회 2008 The Koreran journal of Endocrine Surgery Vol.8 No.1

<B>Purpose: </B>PET-CT is often used to differentiate benign or malignant thyroid incidentalomas. In this retrospective study, we evaluated whether the <SUP>18</SUP>F-FDG uptake pattern and PET-CT findings improved accuracy over the standardized uptake value (SUV). <B>Methods:</B> <SUP>18</SUP>F-FDG PET-CT was performed on 2,178 subjects from August, 2004, to October, 2007, in Sung-ae Hospital. PET-CT was performed on 806 patients (37%) with suspected or known nonthyroidal cancer and 1,372 healthy subjects (63%) without a previous history of cancer. We investigated the clinical characteristics of patients, history, standardized uptake value (SUV), ultrasonography, and hormone levels in blood. Thyroidal cancer was confirmed by ultrasonography-guided fine needle aspiration and pathology after thyroid operation. <B>Results:</B> The prevalence of focal thyroid lesions on PET-CT was 8.8% (191/2178). Thyroid cancer confirmation was 7.9% (15/191). The maximum SUV of malignant thyroid lesions were significantly higher than that of benign lesions (7.00±3.08 vs. 4.49±1.84, P<0.001). <B>Conclusion:</B> PET-CT image interpretation that includes 18F-FDG uptake and SUV is better than PET-CT alone for differentiating benign and malignant lesions. Thyroid cancer risk increases as SUVmax levels increase. <B>(Ko</B><B></B><B>rean J Endocrine Surg 2008;8:38-42)</B>

The Role of Adjuvant Postoperative External Beam Radiotherapy for Locoregional Control in Recurrent Advanced Papillary Thyroid Carcinoma : Preliminary Report in a Single Institute

Sung Ho Park(박성호),Guk Haeng Lee(이국행),Byeong Cheol Lee(이병철),Myung Chul Lee(이명철)Ik Joon Choi(최익준) 대한두경부종양학회 2015 대한두경부 종양학회지 Vol.31 No.2

목적 : 국소 재발한 진행된 갑상선 유두암의 치료로 수술 후 외부방사선의 역할을 알아보고자 하였다. 대상 및 방법 : 원자력병원에서 2000년 1월부터 2012년 2월까지 사이버나이프 혹은 외부방사선 치료를 받은 수술과 방사선요오드 치료 후 국소 재발한 진행된 갑상선 유두암 환자 13 명을 대상으로 하였다. 경과관찰 기간 동안 국소 및 부위 재발이 발생환 환자는 6 명이었다. 몇몇의 위험인자를 찾기 위해서 Kaplan-Meier method와 log-rank test를 이용하여 성별, 종양단계, 방사선 치료 전 수술 횟수, 방사선요오드치료 횟수, 수술 후 잔존 암의 여부, 원격전이에 대해 단변량 분석하였다. 결과 : 방사선 치료 후 평균 추적관찰기관은 53.8개월(범위, 36 ~ 108개월)이고 그중 남자는 4 명이었다. 1년, 2년의 무진행 생존률은 각각 76.9와 53.8%였다. 단병량 분석은 성별과 잔존 암여부(p = 0.0475 and p = 0.0475, 각각)에 따라 무진행 생존률이 진행한 그룹과 진행하지 않은 그룹 사이에 유의한 차이가 있다는 것을 보여줬다. 결론 : 외부 방사선 치료 전에 잔존 암이 없었던 환자의 100%에서 국소 및 부위 재발이 일어나지 않았다. 외부방사선 치료는 수술과 방사선요오드 치료에 반응하지 않는 환자 중 수술 후 잔존 암이 없는 경우에 효과 적인 치료 방법이 될 수 있다.

Stability of yttria-stabilized zirconia during pyroprocessing tests

Choi, Eun-Young,Lee, Jeong,Lee, Sung-Jai,Kim, Sung-Wook,Jeon, Sang-Chae,Cho, Soo Haeng,Oh, Seung Chul,Jeon, Min Ku,Lee, Sang Kwon,Kang, Hyun Woo,Hur, Jin-Mok Elsevier 2016 Journal of Nuclear Materials Vol.475 No.-

<P><B>Abstract</B></P> <P>In this study, the feasibility of yttria-stabilized zirconia (YSZ) was investigated for use as a ceramic material, which can be commonly used for both electrolytic reduction and electrorefining. First, the stability of YSZ in salts for electrolytic reduction and electrorefining was examined. Then, its stability was demonstrated by a series of pyroprocessing tests, such as electrolytic reduction, LiCl distillation, electrorefining, and LiClKCl distillation, using a single stainless steel wire mesh basket containing fuel and YSZ. A single basket was used by its transportation from one test to subsequent tests without the requirements for unloading.</P>

Headache without Cerebrospinal Fluid Leakage

Haeng-Seon Shim,Sung-Il Bae,Seung-Won Choi,Chang-Jin Oh,Myoung-Keun Shin 대한임상노인의학회 2013 대한임상노인의학회지 Vol.14 No.2

요부 경막외 차단후 두통이 발생 할 수 있다. 두통의 원인으로는 저혈압, 경막 천자후 두통, 혈관 주입, 지주막하 출혈과 기뇌증 등이 있다. 본 증례는 63세 된 남자 환자에게 공기를 이용한 저항소실법으로 경막외 차단을 시행하는 도중에 신경학적 증상없이 극심한 두통을 호소한 경우를 보고하고자 한다. 뇌척수액 누출이 없었기 때문에 경막 차단후 두통은 배제하였다. 뇌단층 전산화촬영을 시행하여 기뇌증으로 진단 되어졌다. 적절한 치료후 환자는 회복되었고 입원 4일 만에 퇴원하였다. Headache can occur after a lumbar epidural block. There are several reasons for the development of headache such as hypotension, postdural puncture, vascular injection, subarachnoid hemorrhage and pneumocephalus. We report a case of a 63-year-old male patient who developed a severe headache without neurologic symptoms after a lumbar epidural block with the loss of resistance technique (LORT) using air. We excluded the possibility of postdural puncture headache because there was no cerebrospinal fluid (CSF) leakage. Brain CT was performed and it revealed pneumocephalus. After conservative treatment, the patient recovered and was discharged on the fourth day of hospitalization.

Effect of the<i>crp</i>mutation on the utilization of transferrin-bound iron by<i>Vibrio vulnificus</i>

Choi, Mi-Hwa,Sun, Hui-Yu,Park, Ra-Young,Kim, Choon-Mee,Bai, Young-Hoon,Kim, Young-Ran,Rhee, Joon-Haeng,Shin, Sung-Heui Published by Elsevier/North Holland on behalf of t 2006 FEMS microbiology letters Vol.257 No.2

<P>Cyclic AMP-cAMP receptor protein (CRP) complex plays an essential role in the global regulation of Vibrio vulnificus virulence. We found that growth retardation of V. vulnificus caused by mutation of the crp gene encoding CRP was exacerbated under iron-limited conditions. Accordingly, we investigated the effect of crp mutation on the expression of the vulnibactin-mediated iron-uptake system and the ability of V. vulnificus to utilize transferrin-bound iron, and thus to grow in cirrhotic ascites, a human ex vivo system. The production of vulnibactin was suppressed, and the transcription of the vis and vuuA genes, which encode an enzyme required for vulnibactin synthesis and vulnibactin receptor protein, was also suppressed in the crp mutant. Moreover, the crp mutant could not utilize transferrin-bound iron, and its growth was severely suppressed both on transferrin-bound iron and in cirrhotic ascites. All the defects in the crp mutant were recovered by the in trans complementation of the wild-type crp gene. Putative CRP-binding sequences were found in the regulatory regions of the fur, vis and vuuA genes. These results indicate that crp mutation attenuates the ability to grow on transferrin-bound iron and in a human body fluid by down-regulating the vulnibactin-mediated iron-uptake system.</P>

Amyloid β‐induced elevation of O‐GlcNAcylated c‐Fos promotes neuronal cell death

Choi, Heesun,Kim, Chaeyoung,Song, Hyundong,Cha, Moon‐,Yong,Cho, Hyun Jin,Son, Sung Min,Kim, Haeng Jun,Mook‐,Jung, Inhee BLACKWELL PUBLISHING 2019 Aging Cell Vol.18 No.1

<P><B>Abstract</B></P><P>Alzheimer's disease (AD) is an age‐related neurodegenerative disease characterized by progressive memory loss resulting from cumulative neuronal cell death. O‐linked β‐N‐acetyl glucosamine (O‐GlcNAc) modification of the proteins reflecting glucose metabolism is altered in the brains of patients with AD. However, the link between altered O‐GlcNAc modification and neuronal cell death in AD is poorly understood. Here, we examined the regulation of O‐GlcNAcylation of c‐Fos and the effects of O‐GlcNAcylated c‐Fos on neuronal cell death during AD pathogenesis. We found that amyloid beta (Aβ)‐induced O‐GlcNAcylation on serine‐56 and 57 of c‐Fos was resulted from decreased interaction between c‐Fos and O‐GlcNAcase and promoted neuronal cell death. O‐GlcNAcylated c‐Fos increased its stability and potentiated the transcriptional activity through higher interaction with c‐Jun, resulting in induction of Bim expression leading to neuronal cell death. Taken together, Aβ‐induced O‐GlcNAcylation of c‐Fos plays an important role in neuronal cell death during the pathogenesis of AD.</P>

Desiccation Sensitivity of Tea Seed Embryos and Cryopreservation

Haeng-Hoon Kim,Ju-Won Yoon,Ho-Cheol Ko,Yu-Mi Choi,Hae-Sung Hwang,Yang-Hee Cho,Jae-Gyun Gwag 한국종자연구회 2005 종자과학과 산업 Vol.1 No.2

Genomic Sequence Variability of the Prion Gene (PRNP) in Korean Cattle

Choi, Sang-Haeng,Chae, Sung-Hwa,Choi, Han-Ho,Kim, Jeong-Seon,Kang, Bo-Ra,Yeo, Jung-Sou,Choi, Inho,Lee, Yong-Seok,Choy, Yun-Ho,Park, Hong-Seog Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

In this study, we have investigated sequence variants in the PRNP gene of 20 individuals belonging to the Korean cattle, and have analyzed and compared genetic features between varieties of other cattle breeds. Of the 73 sequence variants identified in Korean cattle, 27 were identified for the first time in this study, whereas 46 of these polymorphisms had previously been isolated. We discovered a 2.6 kb SNP hot spot region localized on the putative promoter region of the PRNP gene. Furthermore, the copy numbers of the octapeptide repeat (24 bp indel) which is detected on the coding sequence (CDS) of the PRNP exhibited a completely homozygous 6/6 genotype which is dominant in other cattle breeds. We also characterized a new 19 bp/10 bp allele located on the putative promoter region of the PRNP gene, which represented 0.71 in allele frequency. To the best of our knowledge, this report is the first to address polymorphisms of the PRNP gene structure in Korean cattle in which BSE has yet to be discovered. Therefore, our findings may prove useful with regard to our current understanding of allelic diversity in bovine species, and may also provide new insights into the genetic factors associated with susceptibility or resistance to BSE.

Optimization of the concentration of autologous serum for generation of leukemic dendritic cells from acute myeloid leukemic cells for clinical immunotherapy

Choi, Bo-Hwa,Kang, Hyun-Kyu,Park, Jung-Sun,Kim, Sang-Ki,Pham, Than-Nhan Nguyen,Zhu, Xiao-Wei,Cho, Duck,Nam, Jong-Hee,Chung, Ik-Joo,Kim, Young-Jin,Rhee, Joon-Haeng,Kim, Hyeoung-Joon,Lee, Je-Jung Wiley-Liss 2006 JOURNAL OF CLINICAL APHERESIS Vol.21 No.4

<P>Clinical application of immunotherapy for acute myeloid leukemia (AML) requires the efficient induction of dendritic cells (DCs) from AML blast cells using in vitro culture. We examined the effect of autologous serum on the properties of leukemic DCs derived from leukemic cells of AML patients by culture in AIM-V medium with GM-CSF, IL-4, TNF-α, and 0, 2, 5, or 10% human autologous serum. The expressions of CD80, CD83, CD86, and HLA-DR were upregulated under all culture conditions; however, 10% autologous serum induced the highest expression levels of several molecules. The capacity of leukemic DCs to stimulate allogeneic T cells increased with increasing serum concentration. Stimulation of autologous CD3+ T cells with leukemic DCs grown in the presence of various concentrations of autologous serum resulted in induction of more IFN-γ-secreting cells than was the case for unprimed CD3+ T cells. Leukemic DCs cultured with 10% autologous serum induced the highest numbers of IFN-γ-secreting cells and CD8+CD56+ T cells from autologous T cells. These results suggest that culture of AML blast cells in the presence of autologous serum could be used to generate leukemic DCs for immunotherapy against AML. The highest serum concentration appeared optimal for generating the most potent leukemic DCs. J. Clin. Apheresis. © 2006 Wiley-Liss, Inc.</P>